Nanoencapsulation of Moringa Olifera Leaves Extract Using a Gelatin Based Matrix via Electrospinning Method
1. Abstract of Applied Sciences and Engineering, 2016, Vol.7
DOI: 10.18488/journal.1001/2016.7/1001.7
7th
International Scientific Conference on Applied
Sciences and Engineering
27-28 February, 2016
Flora Grand Hotel, Dubai
Conference Website: www.scihost.org
7
Paper ID: 16/16/ 7
th
ISCASE
Nanoencapsulation of Moringa Olifera Leaves Extract Using
a Gelatin Based Matrix via Electrospinning Method
Norziah M. Hani1
--- Amir Ehsan Tokarmani2
--- Pablo Juliano3
1
School of Industrial Technology, Universiti Sains Malaysia, Malaysia
2
School of Chemical Sciences, Universiti Sains Malaysia, Malaysia
3
CSIRO, 671 Sneydes Rd, Werribee, VIC 3030, Australia
Abstract
The interest in utilizing phytochemicals from Moringa Olifera (MO) leaves due to its
pharmacological properties has recently increased. However, these properties could
not be harnessed unless extracted and encapsulated. This study aims to evaluate the
extraction of polyphenolic bioactive compounds from MO leaves using aqueous
ethanol (80%). Secondly, nanoencapsulation of extract from MO leaves within fish
gelatin matrix via electrospinning technique was performed to produce nanofibers.
Total phenolics, flavonoids, radical scavenging (IC50 value) and metal scavenging
(FRAP) properties obtained were 67.0±2.5 mg gallic acid equivalents (GAE)/g,
respectively. The electrospinning process was conducted at a flow rate of 0.5 ml/hr,
emitter to collector distance of 10 cm and at 20 kV. The solution consisted of gelatin
(20-45%), acetic acid (30%) and MO extract (1-5%). Optimal encapsulation process
was achieved with 40% gelatin solution containing 3% MO extract was used for fibre
generation. Ultrafine fibres with encapsulated MO extract were successfully produced
with diameters ranging from 20 to 100 nm and 55 to 300 nm with and without the core
material, respectively, as observed through scanning electron microscopy (SEM). The
core material could be visually observed through transmission electron microscopy
(TEM) which coincided with Fourier transform infrared (FTIR) spectrum indicating no
chemical interaction between core and wall material was observed. Atomic force
microscopy (AFM) results indicated that encapsulation process did not have a
significant impact on the gelatin matrix structural attributes. The antioxidative
properties of MO extract illustrated high efficiency of ethanol extraction. This study
demonstrated the potential of nanoencapsulation of plant bioactive extracts through
electrospinning process.
Keywords: Moringa Olifera, Gelatin, Electrospinning Method