En-Te-Hwu (Academia Sinica, Taiwan) - DIY Atomic Force Microscope & Lifelong learning

1. EDWIN 2015/09/18 DIY ATOMIC FORCE MICROSCOPE(AFM) & LIFELONG LEARNING

2. Outline • Different microscopes for different scales • Hands-on learning: DIY AFM • Microscope for children • Lifelong learning: LEGO2NANO summer school • Air pollution (PM 2.5) particles detection • Online science participation: Crowdcrafting

4. Exploring the micro world by an optical microscope Seeing is believing?

5. Diffraction limit of light 100~20k USD We can not “see” the nanoworld

6. Scanning Electron Microscope (SEM) http://www.jeolusa.com/PRODUCTS/ScanningElectronMi croscopes(SEM)/SEM-FIB/JIB- 4600FMultiBeam/tabid/543/Default.aspx 500k~2M USD Image only conductive samples Electron gun Detector Magnetic lens Electron

7. “touching” the nanofeatures …. Image both conductive and non-conductive samples in air, liquid and vacuum environments 50k~500k USD https://en.wikipedia.org/wiki/Atomic_force_microscopy Atomic force microscope (AFM)

8. Exploring the Nano world By a very sharp tip….

9. Year 2013, Francois was looking for an affordable AFM that can be easily built

10. 10 Nano optics replaced by DVD OPU Head Nano scanner replaced by piezo buzzers Metal parts replaced by structures made of PCBs Optical table replaced by handheld anti-vibration table 4 techniques make the AFM affordable Inspired by Sebastian Butefisch

13. 13 The DIY AFM kit http://www.stromlinet-nano.com/ http://www.stromlinet-nano.com/

14. Primary / junior high school children assembling / using the DIY AFM Tearing the parts AFM base Buzzer scanner AFM head Within 2 hours Measuring nanostructures Lego2Nano Lego2Nano Lego2Nano

15. 15 Move the AFM from Clean Room to Class Room

17. Microscope for children in the past 100 years: 1,000x Onion cells Area: 200 x 200 mm Optical microscope for children

18. Microscope for children Now: Magnification 100,000,000 x DNA origami on Mica substrate Area: 5 x 5 mm, AC mode AFM for children DNA origami Sample prepared by Bryan Wei @Tsinghua Universityhttp://www.stromlinet-nano.com/

19. AFM topographic image Area: 5x5 mm Optical image Area: 600x600 mm Measuring surface of an eraser

20. AFM topographic image Area: 15x15 mm Optical image Area: 600x600 mm Measuring fresh leaf surface

21. AFM topographic image Area: 8x8 mm Optical image Area:900x900 mm Measuring data tracks on a DVD disk

22. 22 AFM Measurement 3D printer Nano topography data 3D “Solid data” New idea! Science project demonstration AFM data & 3D printing

23. DIY AFM Laser microscope mode Imaging astrocytes 1000X OM phase contract image with Oil lens (Contrast: 0.143) Laser microscope mode (Contrast: 0.224) 10 mm 10 mm Wesley W. HSIAO,Hsien-Shun LIAO,Hsing-Hung LIN, Yueh-Lun LEE, Chia-Kwung FAN, Chien-Wei LIAO, Po-Yen LIN, En-Te HWU, and Chia-Seng CHANG , Analytical Sciences, 29(9), 885 (2013)

25. LEGO2NANO Summer school 14~28th August 2015

27. Reinvent the DIY AFM

29. Building system design (MIT Media lab)

31. http://aqicn.org/city/beijing/ Unhealthy…..

32. Air pollution (PM 2.5) particles found on the DVD data tracks in Beijing Diameter ~2,300 nm (PM2.5)

34. Crowdcrafting and maybe EpiCollect BY Daniel Lopez Martinez @ LEGO2NANO 2015 SUMMER SCHOOL

35. Thank you !!

36. http://www.stromlinet-nano.com/

En-Te-Hwu (Academia Sinica, Taiwan) - DIY Atomic Force Microscope & Lifelong learning

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