BIOAUGMENTATION IN AQUACULTURE is the supplementation of naturally occurring external microorganisms (bacteria, fungi and yeasts) in shrimp and fish nurseries, ponds and production tanks. The action of bacteria is based on the process of degradation of compounds and the enzymatic action metabolized by these microorganisms to inorganic compounds such as CO2, NO3, SO4 and water. The causes of absence of a diversity and quantity or microorganisms that have a high degradatiion activity can be several, including and inadequate adaption and low reprodution inside the nurseries, ponds and tanks. However, this diversity is not always present (number of species and number of individuals within the same species), even when the minimum conditions necessary for the degrading action of bacteria on the substrate (pH, temperature, nutrients e oxygen). Specifically for the farms (agroindustries) it is common exhalation of strong unpleasant odor and high presencae of non-degraded solids (mud of the bottom of the pond). Among others, the high concentration of ammonia is due to a low microbial activity, in which the microbiota presente in pond is not sufficient to facilitate the treatability of the same In general, the program can be adapted to each of the needs of the production chain, based on the objectives to be achieved and general conditions of each farm. The addition of “ECOMIX” has been shown to be one of the most efficient alternatives to improve productivity, reduce damage caused by diseases (mortality x FCR), and also to preserv the environment (reducing N and P discharges) Particularly, the eutrophication of aquatic systems is caused by release of nitrates, phosphates and nutrients that support the flowering of a huge cell mass of algae in role system (discharged water).

1. BIOTECHNOLOGY AT THE SERVICE OF LIFE

2. WHO WE ARE:
We are a genuinely Brazilian company and we work to ensure the satisfaction of our
customers and partners, creating ties and developing, through respect for the environment,
environmentally friendly products.
The company relies on the founders' experience that has been accumulated over the last
16 years. The entrepreneurs started in the environmental area in 2001 and, now they are
dedicated in their own business, Ecoclã. The family works, based on this acquired experience,
together with its values.
Since then, they carry out their duties so that the company values ​​the environment,
sustainability and product quality. Ecoclã has a commitment to the environment and to society as
a whole.
Technical and scientific quality is always respected without overcoming commercial
issues.

3. Aquaculture Line
ECOCLÃ AND PARTNERS

4. Linha Aquicultura
Goals:
 Offer to the Farmers Consultancy
and Technical Support in Aquaculture
using products with proven quality
and efficiency.
Services Provided:
 Consulting, Technical Support,
technical-financial and environmental
projects.
Aquaculture Line

5. BIOAUGMENTATION IN AQUACULTURE is the supplementation of naturally
occurring external microorganisms (bacteria, fungi and yeasts) in shrimp and
fish nurseries, ponds and production tanks.
The action of bacteria is based on the process of degradation of compounds and
the enzymatic action metabolized by these microorganisms to inorganic
compounds such as CO2, NO3, SO4 and water.
The causes of absence of a diversity and quantity or microorganisms that have a
high degradatiion activity can be several, including and inadequate adaption
and low reprodution inside the nurseries, ponds and tanks.
However, this diversity is not always present (number of species and number of
individuals within the same species), even when the minimum conditions
necessary for the degrading action of bacteria on the substrate (pH,
temperature, nutrients e oxygen).
Aquaculture Line

6. Specifically for the farms (agroindustries) it is common exhalation of strong
unpleasant odor and high presencae of non-degraded solids (mud of the bottom
of the pond). Among others, the high concentration of ammonia is due to a low
microbial activity, in which the microbiota presente in pond is not sufficient to
facilitate the treatability of the same
In general, the program can be adapted to each of the needs of the production
chain, based on the objectives to be achieved and general conditions of each
farm.
The addition of “ECOMIX” has been shown to be one of the most efficient
alternatives to improve productivity, reduce damage caused by diseases
(mortality x FCR), and also to preserv the environment (reducing N and P
discharges)
Particularly, the eutrophication of aquatic systems is caused by release of
nitrates, phosphates and nutrients that support the flowering of a huge cell
mass of algae in role system (discharged water).
Aquaculture Line

7. In addition to complying with current
environmental legislation, the adequacy of
production processes and environmental
management policies are already requirements
of internal and external market.
ECOMIX - G is composed of free living
organisms, naturally occurring and not
handled by genetic engineering 9no
occupational or environment risks).
The product undergo a rigorous quality control
garanteeing the respective concentration of
microorganisms present in in the formulation
and absence of pathogens.
Aquaculture Line

8. Biorremediation:
• Bioaugmentation: introduces specific combinations of microorganisms into a contaminated
environment or into a bioreactor to initiate the bioremediation process.
• Bioestimulation: provides nutrients to populations of autochthonous microorganisms,
increasing their population, promoting growth and increase metabolic activity in the
degradation of contaminants.
Requirements:
 They Microrganisms with metabolic capacity to degrade the contaminant;
 The contaminant must be passable and accessible to the microbial enzymatic processing;
 Satisfactory environmental conditions for the activity of the bioremediator agent.
ECOMIX G x Antimicrobials:
 Treatment response slower, but longer lasting;
 They don’t exert selective pressure leading to pathogen resistance.
 Microbiological composition:
 B. subtilis e B. licheniformis e B. Amyloliquefaciens
Concept and Applications

9. Fundamental Questions
• Conditions for bacterial growth and multiplication
– An Environment containing food (organic load).
– Dissolved oxygen (for aerobic strains) in sufficient
quantities.
– pH between 5 and 9 on the scale.
– Moderate temperature.
Concept and Applications

10. Bacterial growth curve
Lag
Logarithmic
Stationary
Decline
Time
Growth
Pathogenic bacteria
Growth curve of
Bacillus spp

11. • Better distribution of aquatic nutrients;
• Reduction of ammonia and algae proliferation;
• Organic load reduction (BOD);
• O.D’s stabilization in water column na água;
• Elimination of pathogenic vectors (disease);
• Complete digestion of organic sludge
(excrement and leftovers).
Aquaculture Line

12. Application procedures
 When applied  Every 10 days since the beginning of stock, depending on the ammonia
rates observed during the cycle.
 Amount to be applied  Use 46 pound (100 gr) for every 1.000 m², diluted in at least 10
liters of water .
 How to Apply
• Calculate the amount of water to be used and the amount of water to dilute it.
• Mix with water and stir until the GEL is completely dissolved.
• Spread the solution along the whole nursery (areas and ditches with greater accumulation of
organic matter).

13. Aquaculture Line

14. Nursery Application

15. Algae bloom Inoculation procedure

16. Stabilized Environment

17. Saturated Organic Sludge

18. Organics already digested

31. Evaluation of results

32. Linha Aquicultura
• Feed, Growth and FCR

33. Comparative Results – Water quality and production costs.
CONTROL PONDS
Water Quality Analysis:
• pH – Mean variation 6.2 e 8.2
• Maximum monitored toxic ammonia – 0.028 mg/L.
• Transparency (Secchi disk) – 40 cm
• Oxygen – Mean change from 2 mg/L to 8 mg/L.
• Nitrate – 5 mg/L
• Nitrite – 0 mg/L
• Alkalinity – 12 mg/L
• Water Flow – Replacement of infiltration, evaporation
plus 3.600 liter / hour of renovation per tank.
Production data:
• Tilapia’s productivity – 1,3 kg/m².
• Tambacu’s productivity – 1,0 kg/m²
• Average survival – 90% (Tilápia) 85% (Tambacu)
• Feed conversion ratio (Tilápia) – 1,2
• Feed conversion ratio (Tambacu) – 1,6
• Production cost (Tilápia) – $ 2,08 /Kg
• Production cost (Tambacu) – $ 2,25 /Kg
PONDS USING ECOMIX - G
Water Quality Analysis:
• pH – Mean variation 7.2 e 7.6
• Maximum monitored toxic ammonia – 0.014 mg/L.
• Transparency (Secchi disk) – 30 cm
• Oxygen – Mean change from 4,5 mg/L a 6 mg/L.
• Nitrate – 25 mg/L
• Nitrite – 0 mg/L
• Alkalinity – 40 mg/L
• Water Flow – ONLY REPLACEMENT OF
INFILTRATION AND EVAPORATION.
Production data:
• Tilapia’s productivity– 1,52 kg/m².
• Tambacu’s productivity – 1,2 kg/m²
• Average survival – 94% (Tilápia) 90% (Tambacu)
• Feed conversion ratio (Tilápia) – 1,1
• Feed conversion ratio (Tambacu) – 1,5
• Production cost (Tilápia) - $ 1,88 / Kg
• Production cost (Tambacu) – $ 2,03 / Kg

34. Ecoclã Biotecnologia Ltda
comercial@ecocla.com.br
Av.T 2, n°2580, Qd. 5, Lt. 4, Setor Bueno – Goiânia-GO - CEP: 74.215-005
Fone: 55 (62) 3954-5520.