1. Pglo:<br />Green Fluorescent Protein is a gene that expresses for a green fluoresce in organisms. This lab started by cultivating the E. coli, on petri dishes so that they could later be put in the cultivation oven. These bacteria were inserted with buffers and genes so that they could later be expressed on dishes and electrophoresis. This was done with micropipettes. Afterwards, the resulting pool was use for the bacterial application. This process uses burners for protection, petri dishes and the circular applicators for the bacteria. Basically bacteria were taken from the tube with these applicators into the petri dishes and then put into the oven. <br />The next Friday we worked with these bacteria using an electrophoresis. We were supposed to transfer these bacteria that had been soaked in a buffer, into the electrophoresis; but we had technical difficulties. The micropipette tips were too big, and the electrophoresis gel was too thin. Basically we could not do much except load the gel with only White protein and not GFP. After this the gels were washed with comaissie blue and left to be tinted. <br />