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APPLICATION OF FUNGAL PIGMENTS
IN FOOD INDUSTRY
PRESENTED BY:
St. George College of Management and Science
Mousami Jaria
MSc Microbiology
Semester 2
INTRODUCTION
• Among the many microorganisms fungi play
an important role in pigment production that
can be used safely .
• Fungi produce many primary and secondary
metabolites, organic acids, pigments and
other food additives.
• These products of fungi possess many
therapeutic applications like immune
modulators, anticancer, antioxidant etc.
FUNGI GROWN IN LABS FOR PIGMENT
EXTRACTION
• Natural colorants posses antimicrobial activity
and lesser chance of being allergic and are
ecofriendly.
• Fungal pigments are secondary metabolites
that are sometimes produced due to scarcity
in nutrional value.
• Some fungi like Aspergillus, Fusarium,
Penicillium and Trichoderma produce various
pigments as there intermediate metabolites .
• Fungal pigments are classified as carotenoids
and polyketides.
Various fungal pigments and their source
STRUCTURE
APPLICATIONS
• Natural or synthetic colors are very important in
attractive appearance of industrial food making
more attractive.
• Some fungal pigments are used for specific
purposes such as carotenoids protect food and
acts as sunscreen.
• Monascus pigment production:
o Monascus red pigments generally produced as MFR
(monascus fermented rice) improve the organoleptic
characteristics of the food products.
o This pigment contain monocolins which
induces the LDL cholesterol and increases
HDL cholesterol.The common names of this
fungal products are red yeast rice, angkak,
benikoji( japan), red mould (USA).
o Monascus fungi organisms produce angkak
can convert starchy substrates into several
metabolites such as alcohols, fatty acids,
flavour compounds, pigments and vitamins.
Thus its implementation as a coloring
agent in food provides a specific flavor in
product.
• Riboflavin vitamin B2 :
o It is the yellow food colorant and its use is
permitted in many countries.
o Because of its specific affinity it is used mainly for
cereal based products
o Applications of riboflavin is limited due to its bitter
taste and naturally slight odour.
DIFFERENT LEVEL OF RIBOFLAVIN PRODUCTION
• Lycopene production:
o It is red open chain unsaturated carotenoid acyclic
isomer of beta carotene, and longer than any
other carotenoid.
o Lycopene also called psi-carotene is very sensitive
to heat and is insoluble in water.
GENETICALLY MODIFIED FUNGUS:
o Fungus Fusarium sporotrichioide was used to
manufacture colorant and antioxidant lycopene
o Cheap corn material was used as substrate
o Cultures in lab flasks produced 0.5 mg
(lycopene)/g of dry mass within 6 days .
Fusarium sporotrichioide
• Beta-carotene production:
o Beta-carotene is a yellowish carotenoid pigment
also known as pro-vitami. It acts as antioxidant
and has potential positive properties against
certain diseases.
MICROBES USED FOR BETA-CAROTENE PRODUCTION:
o Blakesleatrispora
o Mucor circinelloids
o Phycomyces blakesleeanus
Mucor circinelloides
Phycomyces
blakesleeanus
• Blakesleotrispora: Some strains of this mould
produce high level of beta-carotene. B. trispora
strains are of two types (+) mating type and (-)
mating type. Strains obtained by the specific ratio
mating of above two maating types produce
beta-carotene. Today B. trispora fungal beta-
carotene is produced by two industries, Russia
and Ukraine and second in Leone, Spain.
• Mucorcircinelloides: Wild strains of M.
circinelloides when exposed to the light impulses
blue light, gets activated due to significant change
in structural genes of beta-carotene producing
high level.
• Phyomyces blakesleeanus: Phycomyces is
mainly used for the production of various
chemicals like beta-carotene. They have
enhanced cartenogenic potential when grown
on solid substrates or in liquid media
CONCLUSION
• The kingdom fungi are a diverse group of
prokaryotes. It produces extracellular
metabolites which carry enormous
biotechnological applications.
• The fungal pigment is one of those bio active
compounds. Almost all groups of fungi produce
pigments of different colors and characteristics .
The most important fungal pigments include
carotenoids, flavins, quinones, phenazines etc.
These pigments carry antibacterial, antifungal,
anti cancerous and antioxidant potential.
• The health hazards and environmental
concerns of synthetic pigments compel
researchers to work and find substitute of
such synthetic pigments.
• To overcome hazards of such issues, extracting
fungal pigments and its use could be best
solution, as it takes less effort and cost.
THANK YOU

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Application of fungal pigments in food industry

  • 1. APPLICATION OF FUNGAL PIGMENTS IN FOOD INDUSTRY PRESENTED BY: St. George College of Management and Science Mousami Jaria MSc Microbiology Semester 2
  • 2. INTRODUCTION • Among the many microorganisms fungi play an important role in pigment production that can be used safely . • Fungi produce many primary and secondary metabolites, organic acids, pigments and other food additives. • These products of fungi possess many therapeutic applications like immune modulators, anticancer, antioxidant etc.
  • 3. FUNGI GROWN IN LABS FOR PIGMENT EXTRACTION
  • 4. • Natural colorants posses antimicrobial activity and lesser chance of being allergic and are ecofriendly. • Fungal pigments are secondary metabolites that are sometimes produced due to scarcity in nutrional value. • Some fungi like Aspergillus, Fusarium, Penicillium and Trichoderma produce various pigments as there intermediate metabolites . • Fungal pigments are classified as carotenoids and polyketides.
  • 5. Various fungal pigments and their source
  • 7. APPLICATIONS • Natural or synthetic colors are very important in attractive appearance of industrial food making more attractive. • Some fungal pigments are used for specific purposes such as carotenoids protect food and acts as sunscreen. • Monascus pigment production: o Monascus red pigments generally produced as MFR (monascus fermented rice) improve the organoleptic characteristics of the food products.
  • 8. o This pigment contain monocolins which induces the LDL cholesterol and increases HDL cholesterol.The common names of this fungal products are red yeast rice, angkak, benikoji( japan), red mould (USA). o Monascus fungi organisms produce angkak can convert starchy substrates into several metabolites such as alcohols, fatty acids, flavour compounds, pigments and vitamins. Thus its implementation as a coloring agent in food provides a specific flavor in product.
  • 9.
  • 10. • Riboflavin vitamin B2 : o It is the yellow food colorant and its use is permitted in many countries. o Because of its specific affinity it is used mainly for cereal based products o Applications of riboflavin is limited due to its bitter taste and naturally slight odour.
  • 11. DIFFERENT LEVEL OF RIBOFLAVIN PRODUCTION
  • 12. • Lycopene production: o It is red open chain unsaturated carotenoid acyclic isomer of beta carotene, and longer than any other carotenoid. o Lycopene also called psi-carotene is very sensitive to heat and is insoluble in water. GENETICALLY MODIFIED FUNGUS: o Fungus Fusarium sporotrichioide was used to manufacture colorant and antioxidant lycopene o Cheap corn material was used as substrate o Cultures in lab flasks produced 0.5 mg (lycopene)/g of dry mass within 6 days .
  • 14. • Beta-carotene production: o Beta-carotene is a yellowish carotenoid pigment also known as pro-vitami. It acts as antioxidant and has potential positive properties against certain diseases. MICROBES USED FOR BETA-CAROTENE PRODUCTION: o Blakesleatrispora o Mucor circinelloids o Phycomyces blakesleeanus
  • 16. • Blakesleotrispora: Some strains of this mould produce high level of beta-carotene. B. trispora strains are of two types (+) mating type and (-) mating type. Strains obtained by the specific ratio mating of above two maating types produce beta-carotene. Today B. trispora fungal beta- carotene is produced by two industries, Russia and Ukraine and second in Leone, Spain. • Mucorcircinelloides: Wild strains of M. circinelloides when exposed to the light impulses blue light, gets activated due to significant change in structural genes of beta-carotene producing
  • 17. high level. • Phyomyces blakesleeanus: Phycomyces is mainly used for the production of various chemicals like beta-carotene. They have enhanced cartenogenic potential when grown on solid substrates or in liquid media
  • 18. CONCLUSION • The kingdom fungi are a diverse group of prokaryotes. It produces extracellular metabolites which carry enormous biotechnological applications. • The fungal pigment is one of those bio active compounds. Almost all groups of fungi produce pigments of different colors and characteristics . The most important fungal pigments include carotenoids, flavins, quinones, phenazines etc. These pigments carry antibacterial, antifungal, anti cancerous and antioxidant potential.
  • 19. • The health hazards and environmental concerns of synthetic pigments compel researchers to work and find substitute of such synthetic pigments. • To overcome hazards of such issues, extracting fungal pigments and its use could be best solution, as it takes less effort and cost.