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DNA Fingerprinting for Varietal Identification
- 1. DNA Fingerprinting for Varietal Identification December 30, 2020 Dr. Rajendra Prasad Central Agricultural University, Pusa Ahmed Aquib B.Tech. Biotechnology, VII-Sem Under the Guidance of Dr. Rajeev Kumar and Dr. K.L. Bhutiya
- 2. Headlines
- 3. Varietal Identification ● Protecting Plant Breeder’s Right ○ Unique Genetic Fingerprints (DUS Test) ○ Characterization of Essentially Derived Varieties (EDV) ● Guaranteeing the Authenticity of Crop being Purchased ○ To Farmers and Consumers Smith, J. S. C., and O. S. Smith. "Fingerprinting crop varieties." Advances in agronomy. Vol. 47. Academic Press, 1992. 85-140.
- 4. DNA Profiles / Fingerprints ● Traditionally, varietal identification relied heavily on morphological differences. ● DNA fingerprinting, is regarded as a more objective and, ostensibly, less error-prone method of identifying plant varieties ○ high degree of non-tissue specific polymorphism, ○ minimal influence of environment and, ○ simple inheritance pattern. https://cas.cgiar.org/spia/publications/dna-fingerprinting-crop-varietal-identification-fit-purpose- protocols-their-costs
- 5. Molecular Markers 90s AFLP, RAPD and Adaptations SSR (Simple Sequence Repeats) Highly Polymorphic, Reproducible, Multiallelic, Co-dominant … SNP (Single Nucleotide Repeats) Today https://www.seedtest.org/upload/cms/user/AM2015-June15-0845-1b-Vieriria.pdf
- 6. SSR Markers AGTCTAGCTnnnnnnnnTATATATATATATAnnnnnnnGCTAGCTAGA SSR Markers Primer 1 Primer 2Microsatellite Region Insertion Range With High Preservability (SSR Primer) Simple Repeat Base Sequence Range (SSR) Insertion Range With High Preservability (SSR Primer) Variety A ⚪⚪⚪⚪⚪⚪⚪ ▉ ▉ ▉ ▉ (4 Times) . . . . . . . . . . . . ⚪⚪⚪⚪⚪⚪⚪ Variety B ⚪⚪⚪⚪⚪⚪⚪ ▉ ▉ ▉ ▉ ▉ ▉ ▉ ▉ ▉ (9 Times) ⚪⚪⚪⚪⚪⚪⚪ Variety C ⚪⚪⚪⚪⚪⚪⚪ ▉ ▉ ▉ ▉ ▉ ▉ (6 Times). . . . . . . ⚪⚪⚪⚪⚪⚪⚪
- 7. Overview of Steps For Plant Varietal Identification with SSR Markers
- 8. Rationale for Choosing a Combination of Primers ❏ N = no. of varieties ❏ pi = frequency of the pattern being present in a population ❏ k = no. of primers Tessier, Cécile, et al. "Optimization of the choice of molecular markers for varietal identification in Vitis vinifera L." Theoretical and Applied Genetics 98.1 (1999): 171-177.
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