This document summarizes ovary and ovule culture techniques. Ovary culture involves growing unpollinated flower ovaries in nutrient medium to produce haploid plants through gynogenesis. Ovule culture involves isolating ovules from ovaries and culturing them separately. The techniques allow studying early embryo development, effects of plant hormones, and fruit physiology. Protocols involve surface sterilizing and culturing ovaries or isolating and culturing ovules on solid or liquid nutrient medium. The cultures are incubated at 25°C and can be grown in light or dark conditions. Ovary and ovule culture have applications in plant breeding and research.

1. BRIJLAL BIYANI SCIENCE
COLLEGE
DEPARMENT OF BIOTECHNOLGY
TOPIC:-Induction of callus ,ovary and
Ovule culture
Guided by:-
N.S.Tawani Mam

2. Contents :-
What is callus?
History of ovary and ovule culture
What is ovary and ovule culture
Protocol of ovary and ovule culture
Uses / Application of ovule and ovary culture
Difference between ovary and ovule culture

3. What is callus?
 callus is a growing mass of
unorganized plant parenchyma cells.
 In living plants, callus cells are those cells that cover
a plant wound.

4. History of ovary and ovule culture
• Larue was probably the first to rise aseptic culture of
angiosperm flower.
• Technique of ovary culture was develop by Nitsch in
1951.
• The first report of gynogenesis was given by San Noem in
1976.(in case of barley)

5. Definition of ovary and ovule culture :-
Ovary culture:-
 Ovary culture is the in-vitro technique which is carried outside on a
suitable nutrient medium in an unpollinated flower to grow new
haploid plants.
The process of ovary culture refers as “Gynogenesis” .
Ovule culture:-
Ovule culture is an elegant experimental system by which ovules are
aseptically isolated from the ovary .
 Aseptically grown on chemically defined nutrient medium under
controlled conditions.

6. Fig . Ovary and ovule

7. Selection of either opened or unopened of flower
Wash it with distilled water
Dip in 5% teepol solution/ or any detergent
Wash it 4-5 times with distilled water
Suface sterilization 5% sodium hypochloride (NaoCl)

8. Wash with distilled water
with the of Scalpel separate the ovary
Incubate the culture for 16 hours at 25⁰C

9. Fig. ovary culture

10. Collect the open flower where the anther
are decised and pollination has taken place.
Remove sepals,petals etc from the ovaries
containing either fertilized or unfertilized ovuleA
Soak it in 6% NaoCl solution
Rinse the ovaries 3-4 times times with sterile
distilled water

11. Ovules are gently prodded with the help of spatula by
breaking the funicles at its junction with placental tissue.
Spatula with ovule is gently lowered into the sterile solid or
liquid medium as the culture vial is slanted about 45⁰.
Incubate the ovule either in dark or light for 16 hrs at 25⁰C

13. Egg Cell, Fruit, Ovary, Ovule, Seed

14. Application of ovary and ovule culture :-
It is useful to study the early development of embryo
development .
To study the effect of Phytohormones on parthenocaroic fruits
development.
To study the fruit development and also different type of fruit
physiology

15. References:-
Plant tissue culture (theory and practice,a revised edition)
Authors:-S.S.Bhojwani and M.K.Razdan
Biotechnology Expanding Horizons
Authors:-B.D.Singh
 https://www.google.com/search?q=callus+in+plants&sxsrf=ACYBGNSKahEvXYV3ecomCiyJdxZxInmyYw:158089
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SUtOyPM%253A%252CmHDzN_v7KTm4hM%252C_&vet=1&usg=AI4_-
kR90bTaOGCs1mIP2WGjEIZRph7qog&sa=X&ved=2ahUKEwj47uL6hLrnAhUb8HMBHcjNDT4Q9QEwAHoECAcQA
w&biw=1366&bih=625#imgrc=reujlu-SUtOyPM
 https://pediaa.com/difference-between-ovary-and-ovule/
 https://www.youtube.com/watch?v=5KGaCYXtdYk
 https://www.youtube.com/watch?v=OrZKSGhOl2s
Presented by:- Rahul Joshi
-THANK YOU