This document contains 20 multiple choice questions assessing knowledge of general microbiology topics including: 1) Joseph Lister developed antiseptic surgery 2) Anton van Leeuwenhoek observed microscopic organisms called "animalcules" 3) Louis Pasteur postulated the germ theory of disease 4) Robert Koch introduced the use of sterile culture media and helped prove the germ theory of disease.

1. GENERAL MICROBIOLOGY
1.Who developed the system of antiseptic surgery?
(a)Robert Koch (b)Louis Pasteur
(c)Joseph Lister (d)John Needham
2.Anton van Leeuwenhoek made careful observations of microscopic observations called
(a)Spontaneous generation (b)Animal cules
(c)Germ theory of disease (d) Cholera
3.Who introduced the use of sterile culture media?
(a)Edward Jenner (b)Aristotle
(c)Schwann (d)Spallanzani
4.Edward Jenner introduced vaccination against
(a)Small pox (b)Cow pox
(c)Cholera (d)Polio
5.Who postulated Germ theory of disease?
(a)Ferdinand John (b)Louis Pasteur
(c)John Tyndall (d)Robert Koch
6.Three kingdom classification was introduced in
(a)1969 (b)1926
(c)1866 (d)1852
7.Who proposed a Five kingdom classification?
(a)Ernst Haeckel (b)R. H. Whittaker
(c)Francesco Redi (d)Robert Hooke
8.The Kingdom Protozoa includes
(a)Photosynthetic organisms (b)Eukaryotic organisms
(c)Non-chlorophyllous organisms (d)Prokaryotic organisms
9.Carl Woese introduced
(a)3 Domain Concept (b)Five Kingdom Classification
(c)Three kingdom Classification (d)Spontaneous Generation

2. 10.Which lipids present in the membrane of domain Archea?
(a)Glycerol fatty acyldiester (b)Diacyl glycerol diester
(c)Isoprenoid glycerol diester (d)Diglycerol ester
11.Which have limited role in classification but enormous value in identification?
(a)Molecular tests (b)Serological tests
(c)Biochemical tests (d)DNA-homology
12.The same or different organisms is determined by
(a)% A+T (b)16S rRNA Sequencing
(c)% A+U (d)% G+C
13.How many volumes Bergey’s Manual of Systematic Bacteriology contains?
(a)6 (b)4
(c)3 (d)5
14.In Bergey’s system, Volume I represent _________
(a)Gram – negative bacteria (b)Actinomycetes
(c)Archaeobacteria (d)Gram – positive bacteria
15.In Bergey’s system, Volume III represent _________
(a)Gram – negative bacteria (b)Actinomycetes
(c)Archaeobacteria (d)Gram – positive bacteria
16.In Bergey’s system, volume II contains how many sections?
(a)Sections 1-11 (b)Sections 12-17
(c)Sections 18-25 (d)Sections 26-33
17.In Bergey’s system, volume IV contains how many sections?
(a)Sections 1-11 (b)Sections 12-17
(c)Sections 18-25 (d)Sections 26-33
18.The division of Mycoplasmas is
(a)Tenericutes (b)Mollicutes
(c)Nostocales (d)Prochlorales
19.The Fruiting Gliding Bacteria is also known as
(a)Archaeobacteria (b)Myxobacteria
(c)Mycoplasmas (d)Actinoplanetes

3. 20.Which is act as primary decomposers?
(a)Bacteria (b)Archaea (c)Eukarya (d)Protista