Introduction Principle Instrumentation Application Advantages and disadvantages Conclusion References Fluorescence spectroscopy also known as fluorometry or spectrofluorometry) is a type of electromagnetic spectroscopy that analyzes fluorescence  from a sample. It involves using a beam of light, usually ultraviolet light, that excites the electrons in molecules of certain compounds and causes them to emit light . Devices that measure fluorescence are called fluorometers.

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5/12/2020
By
KAUSHAL KUMAR SAHU
Assistant Professor (Ad Hoc)
Department of Biotechnology
Govt. Digvijay Autonomous P. G. College
Raj-Nandgaon ( C. G. )

2. Introduction
Principle
Instrumentation
Application
Advantages and disadvantages
Conclusion
References
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3.  Fluorescence spectroscopy also known
as fluorometry or spectrofluorometry)
 is a type of electromagnetic spectroscopy that
analyzes fluorescence from a sample.
 It involves using a beam of light,
usually ultraviolet light, that excites the
electrons in molecules of certain compounds
and causes them to emit light .
 Devices that measure fluorescence are
called fluorometers.
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4.  As light of a specific wavelength is passed
through a sample solution, it may be
absorbed by the molecules in that solution.
When it does, the light intensity drops, and
this decrease in light intensity is measured.
 It involves using a beam of light,
usually ultraviolet light, that excites the
electrons in molecules of certain compounds
and causes them to emit light;
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5.  In fluorescence spectrometry both an
excitation spectrum (the light that is
absorbed by the sample) and/or an
emission spectrum (the light emitted by the
sample) can be measured. The
concentration of the analyte is directly
proportional with the intensity of the
emission.
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6. All fluorescence instruments contain three
basic items: a source of light, a sample
holder and a detector.
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8. Very sensitivity
Can be used for quantitation of
fluorescent species
Easy and quick to perform analysis
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9. Fluorescence is a highly sensitive
technique and false positives are likely to
occur unless precautions are taken to
record a genuine signal of the analyte.
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10.  Fluorescence spectroscopy is used in, among others,
biochemical, medical, and chemical research fields for
analyzing organic compounds. There has also been a
report of its use in differentiating malignant skin tumors
from benign.
 Atomic Fluorescence Spectroscopy (AFS) techniques are
useful in other kinds of analysis/measurement of a
compound present in air or water, or other media, such
as CVAFS which is used for heavy metals detection, such
as mercury.
 Fluorescence can also be used to redirect photons,
see fluorescent solar collector.
 Additionally, Fluorescence spectroscopy can be adapted
to the microscopic level using microfluorimetry
 In analytical chemistry, fluorescence detectors are used
with HPLC.
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11. Traditional fluorescence systems were
often inflexible, unwieldy and limited to lab
settings. Today, modular spectroscopy
makes possible dozens of different
fluorescence applications by giving users
the flexibility to combine optical bench
components and accessories in various
configurations
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12. https://www.news-medical.net/life-
sciences/Fluorescence-Spectroscopy.aspx
https://oceanoptics.com/fluorescence-
spectroscopy/
http://www.chromedia.org/chromedia?waxt
rapp=mkqjtbEsHiemBpdmBlIEcCArB&sub
Nav=cczbdbEsHiemBpdmBlIEcCArBP
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