very usefull it

1. V.Kanthasamy
LS14106
Bharathidasan university
Trichy -24 .

2. Introduction
Every chemical compound absorbs,
transmits, or reflects light
(electromagnetic radiation) over a
certain range of wavelength.
Spectrophotometry is widely used for
quantitative analysis in various areas.

3. SPECTROPHOTOMETRY
Spectrophotometry is a method to measure
how much a chemical substance absorbs
light by measuring the intensity of
light as a beam of light passes through
sample solution.
This measurement can also be used to measure
the amount of a known chemical substance.

4. Spectrophotometry is one of the most
useful methods of quantitative analysis
in various fields such as chemistry,
physics, biochemistry, material and
chemical engineering and clinical
applications.

5. DESIGN:

6. The light source shines
The light from the source is directed to the
entrance slit of the monochromator
The monochromator separates the
wavelengths of light
A fraction of the light is transmitted or
reflected from the sample
Focused onto the photodetector sequentially

7. Different Types of
Spectrophotometers:
1.Single Beam:
 In this type, all the light passes through the
sample .
To measure the intensity of the incident light
the sample must be removed so that all the
light can pass through.
This type of spectrometer is usually less
expensive and less complicated.

8. 2.Double Beam:
In this type, before it reaches the sample, the
light source is split into two separate beams.
 From these one passes through the sample
and second one is used for reference.
 This gives an advantage because the
reference reading and sample reading can
take place at the same time.

9. 3.Visible Light (400-700 nm):
Visible spectrophotometers can use
incandescent, halogen, or a combination of
these sources and these spectrophotometers
vary in accuracy.
Plastic and glass cuvettes can be used for
visible light spectroscopy.

10. Applications of a Spectrophotometer:
 It is directly used to measure light intensity at different
wavelengths.
 It is used to determine the unknown concentration of
solution.
 Estimating dissolved organic carbon concentration.
 Specific Ultraviolet Absorption for metric of aromaticity.

11. Reference:
Research methodology
for biological science --
vks