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Similar to Hyperthermia In NS0 6 A1 Cells Results And Conclusion
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Hyperthermia In NS0 6 A1 Cells Results And Conclusion
- 1. Effect of Hyperthermia on NS0 Cells. Robert Fitzpatrick Patrick Conneran
Editor's Notes
- fever range elevation in temperature (i.e. 1-2oC) is thought to positively regulate cell growth (Hasday and Singh, 2000). The positive effect is thought to be due to the influence of temperature on proteins and pathways other than the HSPs, for example, Ras and Rac1 and the proteins PI3K and Akt/PKB. Ras GTPases are membrane bound proteins that play a crucial role in diverse cell physiology (Park et al, 2005). Mild heat stress has been shown to activate Ras and Rac1, both of which regulate processes including cell cycle, transformation, and crucially, thermo-sensitivity.
- The technique used here is the two antibody ‘sandwich’ ELISA . This ELISA is fast and accurate, and if a purified antigen standard is available, the assay can determine the absolute amount of antigen in an unknown sample. The sandwich ELISA requires two antibodies that bind to epitopes that do not overlap on the antigen . This can be accomplished with either two monoclonal antibodies that recognize discrete sites or one batch of affinity-purified polyclonal antibodies – in this case the monoclonal antibody is used, as this is the product of interest.
- Note boteh
- First Table Discounts Replicate 3, second includes it