Why does Staphylococcus probably cause more contamination than Pro Peon Barnum even though most people have higher numbers of the Lauer? Are most agar plates incubated aerobically or anaerobically? Prepare a flowchart that would help you identify your skin biota isolates. Solution The Staphylococcus and Propionibacterium are part of normal microflora of the human skin. Staphylococcus colonizes at various part of the skin as skin comensal, but it can act as opportunistic pathogen when it breaches skin surface and enters the bloodstream. Both organisms are related and can be isolated from the infected part of the skin. Propionibacterium causes mild infections related with skin, but post infectious stage becomes complicated due to the contamination with Staphylococcus. The Propionibacterium is an anaerobic aerotolerent which colonizes mainly on pilosebaceous follicles of the skin. The cellular damages and metabolic byproducts due to the rapid growth of Propionibacterium in the follicle induces the inflammatory reactions in the skin. Later this inflammation makes the affected tissues or cells more susceptible for the colonization of Staphylococcus. It can leads to more complicated chronic stages of infections. The Propionibacterium can rolerate exposure to oxygen for several hours and is capable to survive invitro under anaerobic conditions up to 8 months. Due to the oxygen tolerant charecteristic, Propionibacterium is not reliably detected by aerobic culture because of slow growth. To increase the detection in cultures, it is necessary to make a prolonged aerobic and anaerobic incubation to 14 days in thioglycolate medium and routinely subcultured to agar plates..

1. Why does Staphylococcus probably cause more contamination than Pro Peon Barnum even
though most people have higher numbers of the Lauer? Are most agar plates incubated
aerobically or anaerobically? Prepare a flowchart that would help you identify your skin biota
isolates.
Solution
The Staphylococcus and Propionibacterium are part of normal microflora of the human skin.
Staphylococcus colonizes at various part of the skin as skin comensal, but it can act as
opportunistic pathogen when it breaches skin surface and enters the bloodstream. Both
organisms are related and can be isolated from the infected part of the skin. Propionibacterium
causes mild infections related with skin, but post infectious stage becomes complicated due to
the contamination with Staphylococcus. The Propionibacterium is an anaerobic aerotolerent
which colonizes mainly on pilosebaceous follicles of the skin. The cellular damages and
metabolic byproducts due to the rapid growth of Propionibacterium in the follicle induces the
inflammatory reactions in the skin. Later this inflammation makes the affected tissues or cells
more susceptible for the colonization of Staphylococcus. It can leads to more complicated
chronic stages of infections.
The Propionibacterium can rolerate exposure to oxygen for several hours and is capable to
survive invitro under anaerobic conditions up to 8 months. Due to the oxygen tolerant
charecteristic, Propionibacterium is not reliably detected by aerobic culture because of slow
growth. To increase the detection in cultures, it is necessary to make a prolonged aerobic and
anaerobic incubation to 14 days in thioglycolate medium and routinely subcultured to agar
plates.