1. 天然化合物 PCA對人類非小細胞肺癌生長的影響
長榮大學生物科技學系-101學年度專題實習
實習學生:方睿普
實習單位:高雄醫學大學生物科技學系
指導老師:邱建智老師
Migration assay
介紹 0 hr control 0.03 0.06 0.15 0.3 0.6
對香豆酸(Para-coumaric acid，簡稱PCA)天然
物中以酯型、醚型或配醣體存在。廣泛分佈在繖 -------------------------------------------------------------------------------------------------------------------------
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形科、芸香科、豆科、菊科之植物中；中藥像白 -------------------------------------------------------------------------------------------------------------------------
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芷、茵陳蒿、獨活、秦皮中可發現香豆素酸，在 14 hr
天然物中以苯環對位(para)接hydroxy的結構最常
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見，活性上具有抑制血小板凝集的作用。
研究目的
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(mM)
探討Para-coumaric acid對H1299細胞的影
響，並測試這種藥是否有抑制或是毒殺H1299 35
H1299 cell
細胞的功用。 30
25
Denude zone (%)
研究結果 20
Structures of BPIQ. 15
10
5
0
con 0.03 0.06 0.15 0.3 0.6
PCA dose (mM)
Fig 3. NSCLC H1299 cells were incubated with a series of different
concentrations of PCA for 14 hr.
Morphorgy Invasion assay
control 0.03 0.06
control 0.03 0.06
0.15 0.6 (mM) 0.15 0.3 0.6 (mM)
0.3
Fig 1. NSCLC H1299 cells were incubated with a series of cell number
120
different concentrations of PCA for 24 hr.
viable cells 100
invasion index (%)
80
120
24 hr 60
100 48 hr
40
viable cell number (%)
80
20
60
0
c 0.03 0.06 0.15 0.3 0.6
40 PCA Dose (mM)
Fig 4. NSCLC H1299 cells were incubated in transwell with a
20 series of different concentrations of PCA for 16 hr.
0
Control 0.03 0.06 0.15 0.3 0.6
研究結果
PCA Dose (mM) 在24hr 之前PCA在低濃度(0.03mM、0.06mM)時幾乎無
Fig 2. NSCLC H1299 cells were incubated with a series of different 抑制及毒殺效果，但在高濃度(0.15mM、0.3mM、
concentrations of PCA for 24 and 48 hr. 0.6mM)時則會產生明顯的抑制及毒殺作用。