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VIVA-Tech International Journal for Research and Innovation
ISSN(Online): 2581-7280
Volume 1, Issue 4 (2021)
VIVA Institute of Technology
9th
National Conference on Role of Engineers in Nation Building – 2021 (NCRENB-2021)
www.viva-technology.org/New/IJRI
D-13
Extracting Features from the fundus image using Canny edge
detection method for Pre-Detection of Diabetic Retinopathy
Nishant Dandekar1
, Jayesh Kulkarni2
, Riddhi Raut3
, Karishma Raut4
1
(Electronics and Telecommunication Engineering, VIVA Institute of Technology, Virar, India)
2
(Electronics and Telecommunication Engineering, VIVA Institute of Technology, Virar, India)
3
(Electronics and Telecommunication Engineering, VIVA Institute of Technology, Virar, India)
4
(Electronics and Telecommunication Engineering, VIVA Institute of Technology, Virar, India)
Abstract: Diabetic Retinopathy (DR) is an ailment of the eye caused by diabetes. People suffering from diabetes
can procure the disease. DR is caused when the high blood sugar level damages the blood vessels of the eye. Also
due to high blood sugar level abnormal blood vessels can grow in the retina. This can make the patient lose its
vision. Unfortunately, the symptoms of DR cannot be detected easily. The disease can grow increasingly if left
untreated. Hence it becomes all the more important to detect DR. In this paper we have made use of Image
processing Technique like canny edge detection to extract the features necessary to detect DR and find the severity
of the disease. The features extracted from the image can be used to detect DR by various other methods like SVM,
Logistic Regression, etc.
Keywords – Diabetic Retinopathy, Feature Extraction, Canny Edge Detection, Image Processing Technique
I. INTRODUCTION
Person with diabetes can develop an eye disease called diabetic retinopathy. This is when high blood sugar
causes damage to the blood vessels in the retina. These arteries can become inflamed or leaky, blocking the flow
of blood. Sometimes new abnormal blood vessels grow in the retina. All of these changes can steal patient’s vision.
The symptoms of DR appear gradually over time making it difficult to identify if a particular patient is suffering
from DR or not. Hence it becomes important to identify if a patient who’s having diabetes has DR.
By looking at various papers and journals while researching for Diabetic retinopathy it was seen that the
detection of Diabetic Retinopathy in early stages is difficult as the symptoms are very difficult to detect and test.
Many journals and papers have been written and published on the detection of Diabetic Retinopathy which have
used many different techniques and processes to detect DR. The prediction that the image is having Diabetic
Retinopathy is done by implementing various image processing and machine learning techniques on the extracted
features from the image [1][2].
In this paper, we have used Canny edge detection method to extract important features from the input fundus
image. By using canny edge detection, features like the gaussian image, weak edges, strong edges, magnitude of
the input image is obtained which can be used for detection of Diabetic Retinopathy. This extracted feature can
be used with other Image processing technique like k-means clustering, etc. and Machine learning Techniques
like Conventional neural network, Support Vector Machine (SVM), etc to predict if the input image is having
Diabetic Retinopathy and finding its severity.
VIVA-Tech International Journal for Research and Innovation
ISSN(Online): 2581-7280
Volume 1, Issue 4 (2021)
VIVA Institute of Technology
9th
National Conference on Role of Engineers in Nation Building – 2021 (NCRENB-2021)
www.viva-technology.org/New/IJRI
D-14
II. LITERATURE SURVEY
The collection of different machine learning classifying algorithms on features extracted from output of
different retinal image processing algorithms like diameter of optic disk, lesion specific (microaneurysms,
exudates), image level (pre-screening, AM/FM, quality assessment) were used for prediction of DR in the first
paper. Decision making was performed using SVM, alternating decision tree, Naive Bayes, adaBoost and Random
Forest [2].
An automated method for image-based classification of diabetic retinopathy consisting of 3 stages was
used in Image-Based Classification of Diabetic Retinopathy using Machine Learning. In the first stage image
features were enhanced using 2 image processing technique. On second stage dimensionality of the images and
finds features using the statistical method of principal component analysis was reduced. In the third stage various
machine learning algorithms like the naive Bayes, neural networks, k-nearest neighbors and SVM were used for
classification of images [3].
In research article under title “A Brief Review of the Detection of Diabetic Retinopathy in Human Eyes
Using Pre-Processing & Segmentation Techniques”, it is briefly presented on the detection of DR in the eyes of
people using a variety of pre-screening and classification techniques. There are many ways to separate the blood
vessels present in the retina and if the retinal nerve fibers are separated, one can see if the eyes are affected by DR
or not. The detection depends on the area of the RNFL network. If the total area of the nerve fibre is less, then it
is affected with DR & if the area is more, then the eyes are not affected with the DR and hence it is normal [4].
III. CANNY EDGEDETECTION
The Canny edge detector is an edge detection operator that uses a multi-stage algorithm to find a large range of
edges in images. It was discovered by John F. Canny in 1986. Canny also give a computational theory of edge
detection to prove why the technique works. The Canny edge detection algorithm is consisting of 5 steps:
 Noise reduction
 Gradient calculation
 Non-maximum suppression
 Double threshold
 Edge Tracking by Hysteresis.
After applying these steps, the subsequent result is achieved:
Fig 1. Result obtained after Image Processing
VIVA-Tech International Journal for Research and Innovation
ISSN(Online): 2581-7280
Volume 1, Issue 4 (2021)
VIVA Institute of Technology
9th
National Conference on Role of Engineers in Nation Building – 2021 (NCRENB-2021)
www.viva-technology.org/New/IJRI
D-15
1) Noise Reduction
Since edge detection is susceptible to noise in the image, first step is to remove the eliminate in the image
with a 5x5 Gaussian filter.
2) Finding Intensity Gradient of the Image
The smooth image is filtered through a Sobel kernel in both horizontal and vertical direction to obtained
first derivative in horizontal direction (Gx) and vertical direction (Gy). From these two images, we can find edge
gradient and direction for each pixel as follows:
Edge Gradient(G) : G2x+G2y−−−−−−−√Angle(θ)=tan−1(GyGx)
Gradient direction is always perpendicular to edges. It is rounded to one of four angles representing vertical,
horizontal and two diagonal directions.
3) Non-maximum Suppression
After getting gradient magnitude and direction, a full scan of image is done to remove any unwanted
pixels which may not constitute the edge. For this, at every pixel, pixel is checked if it is a local maximum in its
neighbourhood in the direction of gradient. Check the Fig 2. below:
Fig 2. Edge Detection Non-Maximum Suppression [13]
Point A is on the edge (in vertical direction). Gradient direction is normal to the edge. Point B and C are
in gradient directions. So, point A is checked with point B and C to see if it forms a local maximum. If so, it is
considered for next stage, otherwise, it is suppressed (put to zero). In short, the result you get is a binary image
with "thin edges".
4) Hysteresis Thresholding
This level decides which are all edges are really edges and which are not. For this, we need two threshold
values, minimum Value and maximum Value. Any edges with intensity gradient more than maximum Value are
sure to be edges and those below minimum Value are sure to be non-edges, so discarded. Those who stand between
these two thresholds are categorized edges or non-edges based on their connectivity. If they are connected to "sure-
edge" pixels, they are considered to be part of edges. Otherwise, they are also discarded. See the Fig 3 below:
Fig 3. Hysteresis Thresholding [14]
VIVA-Tech International Journal for Research and Innovation
ISSN(Online): 2581-7280
Volume 1, Issue 4 (2021)
VIVA Institute of Technology
9th
National Conference on Role of Engineers in Nation Building – 2021 (NCRENB-2021)
www.viva-technology.org/New/IJRI
D-16
IV. METHODOLOGY
Design Methodology is important for proper planning and execution of the method. In Design Methodology,
a flowchart of the method is given which gives a short, simple explanation of the paper.
Fig 4. Flowchart of Canny Edge Detection used to extract features.
An input fundus image is sent into the system to extract features. Features are extracted after applying image
processing techniques on it such as gaussian blurring (Noise filtering), Bilateral filtering, contouring. Image
processing techniques like canny edge detector and histogram equalization have been applied to the input images
for detecting their edges, taking their threshold values, increasing the intensity of pixels. This extracted feature
can be used for Detection of Diabetic Retinopathy using various other Image processing and Machine learning
techniques.
Two datasets from two database company are used for the paper. Using two datasets helps to ensure that
the system gets trained for different images from two different databases. Python, was the preferred choice for
the paper. Various application like Anaconda, PyCharm, IntelliJ can be used for Python programming.
1) DIARETdb
The database consists of 130 colour fundus images of which most contain at least mild non-proliferative
signs (Microaneurysms) of the diabetic retinopathy, and some are considered as normal which do notcontain
any signs of the diabetic retinopathy according to all experts who participated in the evaluation.
2) Messidor
The 1200 eye fundus colour numerical images of the posterior pole of the Messidor database were acquired
by 3 ophthalmologic departments using a colour video 3CCD with a 45-degree field of view. Images were
captured using 8 bits per colour plane. The database also provides a excel sheet containing the results of the
image.
V. RESULTS
The image processing techniques are implemented on the input fundus image which are used from two datasets
namely diaretDB, and messidor. Images from datasets are taken as input for image processing technique. The
RGB image is converted into grey scale image before applying canny edge detection algorithm on it. The greyscale
image is shown in Fig 5. A canny edge detection algorithm gives output image containing edges. The results of
images after canny edge detection are shown in Fig 6. and Fig 7. These images are useful in detecting the swelling
veins and red dots inside eye.
VIVA-Tech International Journal for Research and Innovation
ISSN(Online): 2581-7280
Volume 1, Issue 4 (2021)
VIVA Institute of Technology
9th
National Conference on Role of Engineers in Nation Building – 2021 (NCRENB-2021)
www.viva-technology.org/New/IJRI
D-17
Fig 5 Grey scale of an eye image
Fig 6. Canny Edge Detection on Dataset 1 image.
Fig 7. Canny Edge Detection on Dataset 2 image.
VI. CONCLUSION
In this paper, the design and application of a Canny edge detector for detecting small blood vessels from
retinal image is discussed. By changing the threshold value and the sigma value, more clear and accurate features
can be extracted.
After using Canny edge detection technique, features like weak edges, strong edges, magnitude, gaussian
image are extracted from the input image. This features thereby can be used as the base for the detection of
Diabetic Retinopathy from fundus images.
VIVA-Tech International Journal for Research and Innovation
ISSN(Online): 2581-7280
Volume 1, Issue 4 (2021)
VIVA Institute of Technology
9th
National Conference on Role of Engineers in Nation Building – 2021 (NCRENB-2021)
www.viva-technology.org/New/IJRI
D-18
VII. REFERENCES
[1] Subhashini, R., Nithin, T. N. R., & Koushik, U. M. S. Diabetic Retinopathy Detection using Image Processing (GUI).
[2] Bhatia, K., Arora, S., & Tomar, R. (2016, October). Diagnosis of diabetic retinopathy using machine learning classification algorithm. In
2016 2nd International Conference on Next Generation Computing Technologies (NGCT) (pp. 347-351). IEEE.
[3] Conde, P. P., De la Calleja, J., Benitez, A., & Medina, M. A. (2012, November). Image-based classification of diabetic retinopathy using
machine learning. In 2012 12th International Conference on Intelligent Systems Design and Applications (ISDA) (pp. 826-830). IEEE.
[4] Kumaran, Y., & Patil, C. M. (2018). A Brief Review of the Detection of Diabetic Retinopathy in Human Eyes Using Pre-Processing &
Segmentation Techniques. International Journal of Recent Technology and Engineering, 7(4), 310-320.
[5] Chang, Samuel H., et al. "Small retinal vessel extraction using modified Canny edge detection." 2008 International Conference on Audio,
Language and Image Processing. IEEE, 2008.
[6] Dhar, Rajdeep, Radheshyam Gupta, and K. L. Baishnab. "An analysis of CANNY and LAPLACIAN of GAUSSIAN image filters in
regard to evaluating retinal image." 2014 International Conference on Green Computing Communication and Electrical Engineering
(ICGCCEE). IEEE, 2014.
[7] Canny, J. (1986). A computational approach to edge detection. IEEE Transactions on pattern analysis and machine intelligence, (6), 679-
698.
[8] Aquino, A., Gegúndez-Arias, M. E., & Marín, D. (2010). Detecting the optic disc boundary in digital fundus images using morphological,
edge detection, and feature extraction techniques. IEEE transactions on medical imaging, 29(11), 1860-1869.
[9] K. T. Ilayarajaa and E. Logashanmugam, "RetinalBlood Vessel Segmentation usingMorphologicalandCanny Edge Detection Technique,"
2020 International Conference on System, Computation, Automation and Networking (ICSCAN), Pondicherry, India, 2020, pp. 1-5, doi:
10.1109/ICSCAN49426.2020.9262446.
[10] Mendonca, A. M., & Campilho, A. (2006). Segmentation of retinal blood vessels by combining the detection of centerlines and
morphological reconstruction. IEEE transactions on medical imaging, 25(9), 1200-1213.
[11] Gharaibeh, N., Al-Hazaimeh, O. M., Al-Naami, B., & Nahar, K. M. (2018). An effective image processing method for detection of
diabetic retinopathy diseases from retinal fundus images. International Journal ofSignal and Imaging Systems Engineering, 11(4), 206-216.
[12] Xu, Z., Baojie, X., & Guoxin, W. (2017, October). Canny edge detection based on Open CV. In 2017 13th IEEE international conference
on electronic measurement & instruments (ICEMI) (pp. 53-56). IEEE.
[13] R., Rasika & A., Swati & D., Gautami & B., Mayuri & Vaidya, Archana. (2016). Quality Control of PCB using Image Processing.
International Journal of Computer Applications. 141. 28-32. 10.5120/ijca2016909623.
[14] https://docs.opencv.org/3.4/d7/de1/tutorial_js_canny.html

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  • 1. VIVA-Tech International Journal for Research and Innovation ISSN(Online): 2581-7280 Volume 1, Issue 4 (2021) VIVA Institute of Technology 9th National Conference on Role of Engineers in Nation Building – 2021 (NCRENB-2021) www.viva-technology.org/New/IJRI D-13 Extracting Features from the fundus image using Canny edge detection method for Pre-Detection of Diabetic Retinopathy Nishant Dandekar1 , Jayesh Kulkarni2 , Riddhi Raut3 , Karishma Raut4 1 (Electronics and Telecommunication Engineering, VIVA Institute of Technology, Virar, India) 2 (Electronics and Telecommunication Engineering, VIVA Institute of Technology, Virar, India) 3 (Electronics and Telecommunication Engineering, VIVA Institute of Technology, Virar, India) 4 (Electronics and Telecommunication Engineering, VIVA Institute of Technology, Virar, India) Abstract: Diabetic Retinopathy (DR) is an ailment of the eye caused by diabetes. People suffering from diabetes can procure the disease. DR is caused when the high blood sugar level damages the blood vessels of the eye. Also due to high blood sugar level abnormal blood vessels can grow in the retina. This can make the patient lose its vision. Unfortunately, the symptoms of DR cannot be detected easily. The disease can grow increasingly if left untreated. Hence it becomes all the more important to detect DR. In this paper we have made use of Image processing Technique like canny edge detection to extract the features necessary to detect DR and find the severity of the disease. The features extracted from the image can be used to detect DR by various other methods like SVM, Logistic Regression, etc. Keywords – Diabetic Retinopathy, Feature Extraction, Canny Edge Detection, Image Processing Technique I. INTRODUCTION Person with diabetes can develop an eye disease called diabetic retinopathy. This is when high blood sugar causes damage to the blood vessels in the retina. These arteries can become inflamed or leaky, blocking the flow of blood. Sometimes new abnormal blood vessels grow in the retina. All of these changes can steal patient’s vision. The symptoms of DR appear gradually over time making it difficult to identify if a particular patient is suffering from DR or not. Hence it becomes important to identify if a patient who’s having diabetes has DR. By looking at various papers and journals while researching for Diabetic retinopathy it was seen that the detection of Diabetic Retinopathy in early stages is difficult as the symptoms are very difficult to detect and test. Many journals and papers have been written and published on the detection of Diabetic Retinopathy which have used many different techniques and processes to detect DR. The prediction that the image is having Diabetic Retinopathy is done by implementing various image processing and machine learning techniques on the extracted features from the image [1][2]. In this paper, we have used Canny edge detection method to extract important features from the input fundus image. By using canny edge detection, features like the gaussian image, weak edges, strong edges, magnitude of the input image is obtained which can be used for detection of Diabetic Retinopathy. This extracted feature can be used with other Image processing technique like k-means clustering, etc. and Machine learning Techniques like Conventional neural network, Support Vector Machine (SVM), etc to predict if the input image is having Diabetic Retinopathy and finding its severity.
  • 2. VIVA-Tech International Journal for Research and Innovation ISSN(Online): 2581-7280 Volume 1, Issue 4 (2021) VIVA Institute of Technology 9th National Conference on Role of Engineers in Nation Building – 2021 (NCRENB-2021) www.viva-technology.org/New/IJRI D-14 II. LITERATURE SURVEY The collection of different machine learning classifying algorithms on features extracted from output of different retinal image processing algorithms like diameter of optic disk, lesion specific (microaneurysms, exudates), image level (pre-screening, AM/FM, quality assessment) were used for prediction of DR in the first paper. Decision making was performed using SVM, alternating decision tree, Naive Bayes, adaBoost and Random Forest [2]. An automated method for image-based classification of diabetic retinopathy consisting of 3 stages was used in Image-Based Classification of Diabetic Retinopathy using Machine Learning. In the first stage image features were enhanced using 2 image processing technique. On second stage dimensionality of the images and finds features using the statistical method of principal component analysis was reduced. In the third stage various machine learning algorithms like the naive Bayes, neural networks, k-nearest neighbors and SVM were used for classification of images [3]. In research article under title “A Brief Review of the Detection of Diabetic Retinopathy in Human Eyes Using Pre-Processing & Segmentation Techniques”, it is briefly presented on the detection of DR in the eyes of people using a variety of pre-screening and classification techniques. There are many ways to separate the blood vessels present in the retina and if the retinal nerve fibers are separated, one can see if the eyes are affected by DR or not. The detection depends on the area of the RNFL network. If the total area of the nerve fibre is less, then it is affected with DR & if the area is more, then the eyes are not affected with the DR and hence it is normal [4]. III. CANNY EDGEDETECTION The Canny edge detector is an edge detection operator that uses a multi-stage algorithm to find a large range of edges in images. It was discovered by John F. Canny in 1986. Canny also give a computational theory of edge detection to prove why the technique works. The Canny edge detection algorithm is consisting of 5 steps:  Noise reduction  Gradient calculation  Non-maximum suppression  Double threshold  Edge Tracking by Hysteresis. After applying these steps, the subsequent result is achieved: Fig 1. Result obtained after Image Processing
  • 3. VIVA-Tech International Journal for Research and Innovation ISSN(Online): 2581-7280 Volume 1, Issue 4 (2021) VIVA Institute of Technology 9th National Conference on Role of Engineers in Nation Building – 2021 (NCRENB-2021) www.viva-technology.org/New/IJRI D-15 1) Noise Reduction Since edge detection is susceptible to noise in the image, first step is to remove the eliminate in the image with a 5x5 Gaussian filter. 2) Finding Intensity Gradient of the Image The smooth image is filtered through a Sobel kernel in both horizontal and vertical direction to obtained first derivative in horizontal direction (Gx) and vertical direction (Gy). From these two images, we can find edge gradient and direction for each pixel as follows: Edge Gradient(G) : G2x+G2y−−−−−−−√Angle(θ)=tan−1(GyGx) Gradient direction is always perpendicular to edges. It is rounded to one of four angles representing vertical, horizontal and two diagonal directions. 3) Non-maximum Suppression After getting gradient magnitude and direction, a full scan of image is done to remove any unwanted pixels which may not constitute the edge. For this, at every pixel, pixel is checked if it is a local maximum in its neighbourhood in the direction of gradient. Check the Fig 2. below: Fig 2. Edge Detection Non-Maximum Suppression [13] Point A is on the edge (in vertical direction). Gradient direction is normal to the edge. Point B and C are in gradient directions. So, point A is checked with point B and C to see if it forms a local maximum. If so, it is considered for next stage, otherwise, it is suppressed (put to zero). In short, the result you get is a binary image with "thin edges". 4) Hysteresis Thresholding This level decides which are all edges are really edges and which are not. For this, we need two threshold values, minimum Value and maximum Value. Any edges with intensity gradient more than maximum Value are sure to be edges and those below minimum Value are sure to be non-edges, so discarded. Those who stand between these two thresholds are categorized edges or non-edges based on their connectivity. If they are connected to "sure- edge" pixels, they are considered to be part of edges. Otherwise, they are also discarded. See the Fig 3 below: Fig 3. Hysteresis Thresholding [14]
  • 4. VIVA-Tech International Journal for Research and Innovation ISSN(Online): 2581-7280 Volume 1, Issue 4 (2021) VIVA Institute of Technology 9th National Conference on Role of Engineers in Nation Building – 2021 (NCRENB-2021) www.viva-technology.org/New/IJRI D-16 IV. METHODOLOGY Design Methodology is important for proper planning and execution of the method. In Design Methodology, a flowchart of the method is given which gives a short, simple explanation of the paper. Fig 4. Flowchart of Canny Edge Detection used to extract features. An input fundus image is sent into the system to extract features. Features are extracted after applying image processing techniques on it such as gaussian blurring (Noise filtering), Bilateral filtering, contouring. Image processing techniques like canny edge detector and histogram equalization have been applied to the input images for detecting their edges, taking their threshold values, increasing the intensity of pixels. This extracted feature can be used for Detection of Diabetic Retinopathy using various other Image processing and Machine learning techniques. Two datasets from two database company are used for the paper. Using two datasets helps to ensure that the system gets trained for different images from two different databases. Python, was the preferred choice for the paper. Various application like Anaconda, PyCharm, IntelliJ can be used for Python programming. 1) DIARETdb The database consists of 130 colour fundus images of which most contain at least mild non-proliferative signs (Microaneurysms) of the diabetic retinopathy, and some are considered as normal which do notcontain any signs of the diabetic retinopathy according to all experts who participated in the evaluation. 2) Messidor The 1200 eye fundus colour numerical images of the posterior pole of the Messidor database were acquired by 3 ophthalmologic departments using a colour video 3CCD with a 45-degree field of view. Images were captured using 8 bits per colour plane. The database also provides a excel sheet containing the results of the image. V. RESULTS The image processing techniques are implemented on the input fundus image which are used from two datasets namely diaretDB, and messidor. Images from datasets are taken as input for image processing technique. The RGB image is converted into grey scale image before applying canny edge detection algorithm on it. The greyscale image is shown in Fig 5. A canny edge detection algorithm gives output image containing edges. The results of images after canny edge detection are shown in Fig 6. and Fig 7. These images are useful in detecting the swelling veins and red dots inside eye.
  • 5. VIVA-Tech International Journal for Research and Innovation ISSN(Online): 2581-7280 Volume 1, Issue 4 (2021) VIVA Institute of Technology 9th National Conference on Role of Engineers in Nation Building – 2021 (NCRENB-2021) www.viva-technology.org/New/IJRI D-17 Fig 5 Grey scale of an eye image Fig 6. Canny Edge Detection on Dataset 1 image. Fig 7. Canny Edge Detection on Dataset 2 image. VI. CONCLUSION In this paper, the design and application of a Canny edge detector for detecting small blood vessels from retinal image is discussed. By changing the threshold value and the sigma value, more clear and accurate features can be extracted. After using Canny edge detection technique, features like weak edges, strong edges, magnitude, gaussian image are extracted from the input image. This features thereby can be used as the base for the detection of Diabetic Retinopathy from fundus images.
  • 6. VIVA-Tech International Journal for Research and Innovation ISSN(Online): 2581-7280 Volume 1, Issue 4 (2021) VIVA Institute of Technology 9th National Conference on Role of Engineers in Nation Building – 2021 (NCRENB-2021) www.viva-technology.org/New/IJRI D-18 VII. REFERENCES [1] Subhashini, R., Nithin, T. N. R., & Koushik, U. M. S. Diabetic Retinopathy Detection using Image Processing (GUI). [2] Bhatia, K., Arora, S., & Tomar, R. (2016, October). Diagnosis of diabetic retinopathy using machine learning classification algorithm. In 2016 2nd International Conference on Next Generation Computing Technologies (NGCT) (pp. 347-351). IEEE. [3] Conde, P. P., De la Calleja, J., Benitez, A., & Medina, M. A. (2012, November). Image-based classification of diabetic retinopathy using machine learning. In 2012 12th International Conference on Intelligent Systems Design and Applications (ISDA) (pp. 826-830). IEEE. [4] Kumaran, Y., & Patil, C. M. (2018). A Brief Review of the Detection of Diabetic Retinopathy in Human Eyes Using Pre-Processing & Segmentation Techniques. International Journal of Recent Technology and Engineering, 7(4), 310-320. [5] Chang, Samuel H., et al. "Small retinal vessel extraction using modified Canny edge detection." 2008 International Conference on Audio, Language and Image Processing. IEEE, 2008. [6] Dhar, Rajdeep, Radheshyam Gupta, and K. L. Baishnab. "An analysis of CANNY and LAPLACIAN of GAUSSIAN image filters in regard to evaluating retinal image." 2014 International Conference on Green Computing Communication and Electrical Engineering (ICGCCEE). IEEE, 2014. [7] Canny, J. (1986). A computational approach to edge detection. IEEE Transactions on pattern analysis and machine intelligence, (6), 679- 698. [8] Aquino, A., Gegúndez-Arias, M. E., & Marín, D. (2010). Detecting the optic disc boundary in digital fundus images using morphological, edge detection, and feature extraction techniques. IEEE transactions on medical imaging, 29(11), 1860-1869. [9] K. T. Ilayarajaa and E. Logashanmugam, "RetinalBlood Vessel Segmentation usingMorphologicalandCanny Edge Detection Technique," 2020 International Conference on System, Computation, Automation and Networking (ICSCAN), Pondicherry, India, 2020, pp. 1-5, doi: 10.1109/ICSCAN49426.2020.9262446. [10] Mendonca, A. M., & Campilho, A. (2006). Segmentation of retinal blood vessels by combining the detection of centerlines and morphological reconstruction. IEEE transactions on medical imaging, 25(9), 1200-1213. [11] Gharaibeh, N., Al-Hazaimeh, O. M., Al-Naami, B., & Nahar, K. M. (2018). An effective image processing method for detection of diabetic retinopathy diseases from retinal fundus images. International Journal ofSignal and Imaging Systems Engineering, 11(4), 206-216. [12] Xu, Z., Baojie, X., & Guoxin, W. (2017, October). Canny edge detection based on Open CV. In 2017 13th IEEE international conference on electronic measurement & instruments (ICEMI) (pp. 53-56). IEEE. [13] R., Rasika & A., Swati & D., Gautami & B., Mayuri & Vaidya, Archana. (2016). Quality Control of PCB using Image Processing. International Journal of Computer Applications. 141. 28-32. 10.5120/ijca2016909623. [14] https://docs.opencv.org/3.4/d7/de1/tutorial_js_canny.html