seminar ppt.

1. DNA Origami as a Drug
Delivery System
By : Eman Mohamed Abdelrazik
ID: 50371
Under the supervision of :
Dr. Ahmed Ibrahim Abd elmaksoud
(BTSM 402)

2. Outlines

3. Is this the only structure of
DNA?!!

5. Richard Feynman, Nobel Prize in Physics 1965 .
What I can not create, I do not
understand !!

6. DNA Origami
 The nanoscale folding of DNA to create
non-arbitrary two- and three-dimensional
shapes at the nanoscale.

7. Building
DNA
Origami

8. 1. DNA Scaffold
- M13mp8 vector
- ssDNA

9. 2.DNA Staples
 Staple= Short Oligonecleotides
Each staple go to unique position in the
origami.

10. 2.DNA Staples
 Sequence Design by V-Helix Algorithm.
 Software: CanDo
http://cando-dna-origami.org/

11. Bridges of königsberg

12. 3. DNA Tiles
(Wei et al.,2012)

13. Holiday Junction
Holiday Junction = Crossing Over
 Multiple immobile Holliday junctions provide
nanostructure with a high degree of
structural rigidity.

14.  The DNA double crossover (DX)
has two Holliday junctions which
robust connections among several
DNA helices.
ScaffoldStaples

15. 4. DNA Lattice
Rectangular tile Y-shaped tile X-shaped tile Star shaped tile
(Roh et al., 2011)

16. (Zhang et al., 2008)

17. (Kumar et al., 2016)

18. (Beanson et al., 2015)

19. DNA Origami Applications

20. DNA Nano-Robots
Clamshell Naorobots used as Drug Delivery
System (Douglas et al., 2012).

21. Targeting Mechanism
Aptamer-
based lock.
(Charoenphol and Bermudez, 2014)

22. Cell Selectivity
DNA Nanorobots Cancer cellNon-Cancer cell
DNA loaded by Doxorubcin(Doxo) for
treatment of cancer cells.

23. Cytotoxicity of DNA Origami
(Chang et al., 2011)

24. (Jiang et al., 2012)

25. Non-lipid
encapsulation
50 nm
Vs.
lipid
encapsulation
 Half-life of 370 ± 38 min
comparable to similarly
formulated PEGylated
liposomes.

26. Biotechnology applications
of DNA origami
(Linko et al., 2015)

27. Conclusions

29. Biotechnology
Nanotechnology

30. Cell Selectivity
Nuclease resistance
Non-toxic & Non- immunogenic

31. High Cost
Stability

34. Thank You