raman spectroscopy

2. Identification Of Pharmaceuticals By Raman Spectroscopy

3. Pharmaceutical Drug
A compound manufactured for use
as a medicinal drug
Composition of pharmaceutical
drug
• Active Pharmaceutical Ingredient
(API)
• Excipients

4. Formulation of
drug
Enteral Formulation
Parenteral Formulation
Topical Formulation

5. Why to identify pharmaceuticals?
Confirmation of structure of API
Intrinsic properties of API
Solid state properties of API
Bulk properties
Mislabeling
Defects

6. Types Of Defects in Counterfeit Medicine

7. Why Raman Spectroscopy Is Used?
Non _destructive technique.
Vibrational spectroscopy
No sample preparation
Elastic and inelastic scattering.
Net change in Polarizability

8. Fundamental Structural
Investigation
Raman band Assignments
Structural detail of various
families of compounds such as
narcotic and illicit drugs.
Example: Paracetamol

9. Band assignments in paracetamol
1650–300 cm−1
Intensity and
position
1649 cm−1 →
1639 cm−1
(Amide bond)

10. Quantitative Analysis
Measurement of
the quantities of
the particular
constituents
present in a
substance.
Determine
polymorphic
impurities with
a limit of
quantitation
<2%
Example:
Paracetamol
form I and II.

11. Difference
• 1650cm-1
• 1625cm-1
• 1609cm-1
Similarity
• 1169cm-1

12. Scatter plot

13. Loading
The loading plot graphs the coefficients of each variable for the first
component versus the coefficients for the second component.
Use the loading plot to identify which variables have the largest effect
on each component.
Range from -1 to 1.
 Loadings close to -1 or 1 = Strong influence
Loadings close to 0 =Weak in fluence .

15. The End
Thank
You