Zebrafish are widely used as a model organism in biomedical research. They are useful for studying the toxic effects of chemicals in water and as a model for atherosclerosis and other conditions. Researchers were able to reproduce early stages of atherosclerosis by feeding zebrafish a high-cholesterol diet. This suggests zebrafish are suitable for studying inflammatory processes in early atherosclerosis development. The document also discusses using zebrafish to study angiogenesis, the effects of nicotine, and evaluating nanoparticle biodistribution and efficacy.
Why Using Zebrafish for BioMedical Research and Drug Discovery?Jens-Ole Bock
Zebrafish is a vertebrate whose genome is completely sequenced; who shares a similar physiology to humans, including many equivalent organs; who offers with a growing battery of genetic tools, which combined with organism’s transparency, permits the tracing of cells and easy genetic manipulation (CRISPR).
These characteristics help ensure that information acquired through zebrafish is more accurate and informative than that obtained by in vitro assays, and thus easier to extrapolate to human biology.
Powerpoint accompanying research paper on the Teratogenic effects of Fipronil (GABAergic antagonist) on the neurological development of zebrafish (as model of vertebrates).
Why Using Zebrafish for BioMedical Research and Drug Discovery?Jens-Ole Bock
Zebrafish is a vertebrate whose genome is completely sequenced; who shares a similar physiology to humans, including many equivalent organs; who offers with a growing battery of genetic tools, which combined with organism’s transparency, permits the tracing of cells and easy genetic manipulation (CRISPR).
These characteristics help ensure that information acquired through zebrafish is more accurate and informative than that obtained by in vitro assays, and thus easier to extrapolate to human biology.
Powerpoint accompanying research paper on the Teratogenic effects of Fipronil (GABAergic antagonist) on the neurological development of zebrafish (as model of vertebrates).
Rapid In Vivo Assessment of Bioactivity in Zebrafish: High Content Data for P...OSU_Superfund
Dr. Robert Tanguay's presentation on April 30, 2014 with the 21st Century Toxicology Seminar Series of the California Dept. of Pesticide Regulation. https://www.facebook.com/media/set/?set=a.766268766739722.1073741858.440748475958421&type=3&uploaded=5
For more information about the research of Robert Tanguay, visit the Superfund Research Program: http://superfund.oregonstate.edu and the Environmental Health Science Center: http://ehsc.oregonstate.edu
Researcheson.zebra.fish,their origin,their role in certain experiments and thus used as model.it is used in heart disease, and their cardiovascular disease
Developing Zebrafish Models of Complex Phenotypes Relevant to Human Brain Dis...Jonathan Cachat
Dissertation Defense Presentation
Jan 2013 - Tulane University, Neuroscience Graduate Program
Jonathan M. Cachat
Committee:
Allan V. Kalueff, Ph.D.
Jill Daniel, Ph.D.
David Corey, Ph.D
Benjamin Hall, Ph.D.
The use of genetic engineering technology in animals has been associated with ethical issues, some of which relate to animal welfare. Discuss examples of genetically engineered animals and evaluate the ethical concerns of genetic engineering.
This pdf file tries to answer the question as to why model organisms are used is research. By definition, Model organisms are a group of species of organisms that have been studied extensively, largely because they are easy to maintain under controlled laboratory conditions. The reason for them being studied is usually because they possess a number of experimental advantages.
Spermatotoxic impact of bonny light crude oil (BLCO) ingestion on adult male ...lukeman Joseph Ade shittu
Increasing concern has been expressed about the possible declining trend in the sperm quality and sperm count of man as a result of exposure to environmental estrogenic agents in the past few years now. There is a general paucity of knowledge of BLCO ingestion on the reproductive effect. Hence, we aim to evaluate the impact of sub-lethal dose of BLCO ingestion on semen parameters of adult male mice. Initial acute toxicity study was carried out to determine the lethal dose of BLCO, which was calculated to be 37.4 mg/Kg body wt. A sub-lethal dose of 20 mg/Kg bwt /day of BLCO were then given to 8 male mice in the experimental group. While, the control group of 7 animals received equal volume of 0.9% normal saline via oral garvage for 2 weeks. Data were analysed using SPSS 12 statistical software with P < 0.05 considered statistically significant. There was a significant (P < 0.05) weight gain in the treated group with a significant (P < 0.05) reduction in sperm motility in the treated compared with control. The sperm density of treated and control were 14.5 x 106 /ml and 20.5 x 106 /ml respectively. However, there were also no significant difference in the relative testicular weight and sperm density of treated from that of the control respectively. Thus, it was concluded that BLCO ingestion is spermatotoxic in the adult male Swiss mice
Rapid In Vivo Assessment of Bioactivity in Zebrafish: High Content Data for P...OSU_Superfund
Dr. Robert Tanguay's presentation on April 30, 2014 with the 21st Century Toxicology Seminar Series of the California Dept. of Pesticide Regulation. https://www.facebook.com/media/set/?set=a.766268766739722.1073741858.440748475958421&type=3&uploaded=5
For more information about the research of Robert Tanguay, visit the Superfund Research Program: http://superfund.oregonstate.edu and the Environmental Health Science Center: http://ehsc.oregonstate.edu
Researcheson.zebra.fish,their origin,their role in certain experiments and thus used as model.it is used in heart disease, and their cardiovascular disease
Developing Zebrafish Models of Complex Phenotypes Relevant to Human Brain Dis...Jonathan Cachat
Dissertation Defense Presentation
Jan 2013 - Tulane University, Neuroscience Graduate Program
Jonathan M. Cachat
Committee:
Allan V. Kalueff, Ph.D.
Jill Daniel, Ph.D.
David Corey, Ph.D
Benjamin Hall, Ph.D.
The use of genetic engineering technology in animals has been associated with ethical issues, some of which relate to animal welfare. Discuss examples of genetically engineered animals and evaluate the ethical concerns of genetic engineering.
This pdf file tries to answer the question as to why model organisms are used is research. By definition, Model organisms are a group of species of organisms that have been studied extensively, largely because they are easy to maintain under controlled laboratory conditions. The reason for them being studied is usually because they possess a number of experimental advantages.
Spermatotoxic impact of bonny light crude oil (BLCO) ingestion on adult male ...lukeman Joseph Ade shittu
Increasing concern has been expressed about the possible declining trend in the sperm quality and sperm count of man as a result of exposure to environmental estrogenic agents in the past few years now. There is a general paucity of knowledge of BLCO ingestion on the reproductive effect. Hence, we aim to evaluate the impact of sub-lethal dose of BLCO ingestion on semen parameters of adult male mice. Initial acute toxicity study was carried out to determine the lethal dose of BLCO, which was calculated to be 37.4 mg/Kg body wt. A sub-lethal dose of 20 mg/Kg bwt /day of BLCO were then given to 8 male mice in the experimental group. While, the control group of 7 animals received equal volume of 0.9% normal saline via oral garvage for 2 weeks. Data were analysed using SPSS 12 statistical software with P < 0.05 considered statistically significant. There was a significant (P < 0.05) weight gain in the treated group with a significant (P < 0.05) reduction in sperm motility in the treated compared with control. The sperm density of treated and control were 14.5 x 106 /ml and 20.5 x 106 /ml respectively. However, there were also no significant difference in the relative testicular weight and sperm density of treated from that of the control respectively. Thus, it was concluded that BLCO ingestion is spermatotoxic in the adult male Swiss mice
PROJECT DESCRIPTION Dose protein level affect the metabolic rate.docxbriancrawford30935
PROJECT DESCRIPTION
Dose protein level affect the metabolic rate of zebra fish?
STATEMENT OF THE PROBLEM
dietary ingredients, nutrients as well as anti-nutritional factors are important factors that affect its growth and development. However, there is lack of proper nutritional control due to absence of standardized reference diet (Boyle 5354). Moreover, many epidemiological studies indicate that several prenatal and peri-natal dies are important in the growth and development of Zebra fish (Danio rerio) (Siccardi et al 23). In order to provide standardized dietary framework, there is need for the provision of specific dietary and nutritional standard to improve the growth and development of Zebra fish (Danio rerio). Thus, the need for investigating the growth and metabolic rate of Zebra fish (Danio rerio) when fed with different commercial diets.
BACKGROUND
How protein can affect the metabolic rate?
Does the oxygen level in the water indicate the metabolic rate?
STUDY SPECIES ( Zebra Fish)
Zebra fish (Danio rerio) is a small shoaling fish and it belongs to the minnow family of Cyprinadae and order cypriniformes. Due to its unique characteristics, the Zebra fish (Danio rerio) is mostly used as a vertebrae model organism in scientific research. It has regenerative qualities and has been modified by various researchers to produce several transgenic strains. It has been used in various medical research studies and findings including those dealing with the prevention of cancer, cardio-vascular conditions as well as investigation of the immune systems and other drug discovery systems
Furthermore, due to their omnivores qualities, they are have been used in environmental monitoring activities in order to prevent water pollution by estrogen (Howard 1173). Based on previous literatures and research studies investigating their growth and metabolic rate, it is evident that the Zebra fish (Danio rerio) are imperative in studying the development, genetics as well as the human disease conditions (Williams et al 153).
HYPOTHESES, GOALS, AND OBJECTIVES
HYPOTHESES: Protein will increase the metabolic rate.
GOALS: To test the metabolic rate after using 6 different protein level in commercial diet by measuring the oxygen level
OBJECTIVES: ??
METHODS
I will use 6 commercial diet with different level of protein and the same level of fat and fibers
Group 1
Name
protein
Fat
Fiber
Nutrafin Max Flake Food
44%
5%
2%
Tetra Pond Koi Vibrance
31%
5%
2%
Group 2
Name
Protein
Fat
Fiber
TetraMin Tropical Flakes
38%
6%
6%
HBH Algae Grazers
28%
6%
6%
Name
Protein
Fat
Fiber
New Life Spectrum Premium
48%
5%
4%
Dainichi Veggie Deluxe
28%
5%
4%
I will use 3 tanks each tank have 2 male Zebra Fish. (Find the different between male and female metabolic rate) (Why did I choose male??)
I will measure the oxygen level by using
Salifert Oxygen Test Kit
Quick and accurate test for dissolved oxygen content. Proper oxygen concentration is vital for aquarium inhab.
ABSTRACT- The present study was conducted to investigate the effect of cadmium chloride on Histoarchiteceture of head kidney of fresh water fish Heteropneustes fossilis. The fishes were exposed to 0.5 ppm of cadmium chloride for 21 days. The most remarkable changes in head kidney, due to cadmium chloride were lysed condition of interrenal and chromaffin cells. The traces of cytoplasm had dark brown to black coloured cytoplasm. Most of cells are deformed and necrotic condition. Their size was significant at (P< 0.01 and 0.001) increased after cadmium chloride. All these changes will be recovered by herbal compound i.e. Ashwagandha. The damaged tissues were recovered in already treated group.
Key-words- Ashwagandha, Cadmium chloride, Chromaffin cells, Heteropneustes fossilis, Histopathology, Interrenal cells
Vibriosis is one of the most prevalent fish diseases caused by bacteria belonging the genus Vibrio affecting many marine and fresh water fishes. The disease characterized by septicemia, dermal ulceration, ascitis and haematopiotic necrosis.
DOI:10.21276/ijlssr.2016.2.4.20
ABSTRACT- The present investigation was carried out to the effect of dimethoate on histopathological changes in
kidney of freshwater fish, Garra mullya. Fishes was exposed to sub lethal concentration of dimethoate (0.0238ppm of
96hrs.) for 7, 14, 21 days. Fishes exposed to dimethoate were characterized by loosening of haemopoietic tissue,
uriniferous tubules have lost their original appearance, vacuolated cytoplasm, degeneration in the epithelial cells of renal
tubule, narrowing of the tubular lumen and damaged glomeruli. The lesions in the vital organ might have resulted in
physiological and metabolical dysregulations. In chronic treatment of dimethoate exposure may pose serious threat to fish
health and affect their population. Key-words- Dimethoate, Histopathology, Kidney, Garra mullya
Luis Alberto Velasquez Cumplido
Grado académico: Doctor en Ciencias Biológicas, Mención Ciencias Fisiológicas
Institución: Pontificia Universidad Católica (PUC)
Fecha: 28 de Noviembre de 1997
LINEAS DE INVESTIGACIÓN
Mi foco de investigación se ha centrado en el uso de herramientas de biología molecular, celular y nanociencia para abordar problemas biomédicos básicos y aplicados. Mis proyectos básicos se centran en caracterizar las relaciones huésped hospero a nivel celular y molecular. Mis proyectos aplicados se centran en el uso de la nanopartículas para la liberación de compuestos con actividad biológica.
En los últimos 10 años he publicado 33 manuscritos en revistas con comité editorial, he participado en la presentación de 5 patentes y he presentado numerosos resúmenes en congresos nacionales e internacionales. Soy revisor de numerosas revistas nacionales e internacionales y mi laboratorio ha recibido numerosas distinciones a la excelencia científica.
Respecto a mis proyectos, soy director de Biomedicina del proyecto basal en Nanociencia y Nanotecnología CEDENNA. Me adjudique un proyecto a centros científicos de excelencia en la Wellcome trust, Inglaterra. Tengo un FONDECYT regular y un PBCT y soy subdirector de un proyecto INNOVA.
En mis proyectos colaboro con Robert Lange, del MIT, USA, con Andrew Sharkey de la Universidad de Cambridge y el Dr. Myron Cristodoulides de la Universidad de Southampton. En mi laboratorio he dirigido 14 tesis de pregrado, 4 de postgrado y un post-doctorado.
Non invasive high resolution in vivo imaging of alpha-naphthylisothiocyanate
(ANIT) induced hepatobiliary toxicity in STII medaka
Ron Hardman∗, Seth Kullman, Bonny Yuen, David E. Hinton
Duke University, Nicholas School of the Environment and Earth Sciences, Durham, NC 27708, United States
International Journal of Engineering and Science Invention (IJESI)inventionjournals
International Journal of Engineering and Science Invention (IJESI) is an international journal intended for professionals and researchers in all fields of computer science and electronics. IJESI publishes research articles and reviews within the whole field Engineering Science and Technology, new teaching methods, assessment, validation and the impact of new technologies and it will continue to provide information on the latest trends and developments in this ever-expanding subject. The publications of papers are selected through double peer reviewed to ensure originality, relevance, and readability. The articles published in our journal can be accessed online.
Developmental Anomalies and Oxidative Stress Responses in Zebrafish (Danio Re...Premier Publishers
The discharge of human pharmaceuticals via wastewater treatment plants represents a major threat to non-target aquatic organisms since they are continually exposed throughout their lifespan. The individual effects of the anaesthetic, lidocaine; the cytostatics, ifosfamide and cyclophosphamide; and the antimicrobials, sulfamethoxazole, amoxicillin and erythromycin on 24 hpf (hours post fertilization) zebrafish (Danio rerio) following a 96-h exposure was investigated by evaluating embryonic development, catalase (CAT) enzyme activity, and the gene expressions of CAT, cytosolic superoxide dismutase (SOD1), and mitochondrial superoxide dismutase (SOD2). Lidocaine, cyclophosphamide and sulfamethoxazole induced neurotoxicity (scoliosis, tail malformation) and cardiotoxicity (pericardial edema, bradycardia) in the zebrafish which correlate with their adverse effects in mammals. These observations were linked to oxidative stress as indicated by the significant alteration of CAT activity and amounts of transcripts of SOD1, SOD2, and CAT. The CAT activity and gene expressions of the antioxidants were significantly upregulated at sublethal and levels of ifosfamide, lidocaine, cyclophosphamide, and erythromycin non-toxic to embryonic development in this study which suggest that these antioxidants may play a role in the prevention of teratogenicity in the larvae. The multi-level effect approach adopted in this study provides a better understanding of the mechanisms of toxicity employed by lidocaine, cyclophosphamide and sulfamethoxazole towards zebrafish. This study shows the importance of evaluating stress biomarkers and emphasizes the need for chronic studies in assessing pharmaceutical effects on aquatic organisms.
Francesca Gottschalk - How can education support child empowerment.pptxEduSkills OECD
Francesca Gottschalk from the OECD’s Centre for Educational Research and Innovation presents at the Ask an Expert Webinar: How can education support child empowerment?
Read| The latest issue of The Challenger is here! We are thrilled to announce that our school paper has qualified for the NATIONAL SCHOOLS PRESS CONFERENCE (NSPC) 2024. Thank you for your unwavering support and trust. Dive into the stories that made us stand out!
Palestine last event orientationfvgnh .pptxRaedMohamed3
An EFL lesson about the current events in Palestine. It is intended to be for intermediate students who wish to increase their listening skills through a short lesson in power point.
The French Revolution, which began in 1789, was a period of radical social and political upheaval in France. It marked the decline of absolute monarchies, the rise of secular and democratic republics, and the eventual rise of Napoleon Bonaparte. This revolutionary period is crucial in understanding the transition from feudalism to modernity in Europe.
For more information, visit-www.vavaclasses.com
June 3, 2024 Anti-Semitism Letter Sent to MIT President Kornbluth and MIT Cor...Levi Shapiro
Letter from the Congress of the United States regarding Anti-Semitism sent June 3rd to MIT President Sally Kornbluth, MIT Corp Chair, Mark Gorenberg
Dear Dr. Kornbluth and Mr. Gorenberg,
The US House of Representatives is deeply concerned by ongoing and pervasive acts of antisemitic
harassment and intimidation at the Massachusetts Institute of Technology (MIT). Failing to act decisively to ensure a safe learning environment for all students would be a grave dereliction of your responsibilities as President of MIT and Chair of the MIT Corporation.
This Congress will not stand idly by and allow an environment hostile to Jewish students to persist. The House believes that your institution is in violation of Title VI of the Civil Rights Act, and the inability or
unwillingness to rectify this violation through action requires accountability.
Postsecondary education is a unique opportunity for students to learn and have their ideas and beliefs challenged. However, universities receiving hundreds of millions of federal funds annually have denied
students that opportunity and have been hijacked to become venues for the promotion of terrorism, antisemitic harassment and intimidation, unlawful encampments, and in some cases, assaults and riots.
The House of Representatives will not countenance the use of federal funds to indoctrinate students into hateful, antisemitic, anti-American supporters of terrorism. Investigations into campus antisemitism by the Committee on Education and the Workforce and the Committee on Ways and Means have been expanded into a Congress-wide probe across all relevant jurisdictions to address this national crisis. The undersigned Committees will conduct oversight into the use of federal funds at MIT and its learning environment under authorities granted to each Committee.
• The Committee on Education and the Workforce has been investigating your institution since December 7, 2023. The Committee has broad jurisdiction over postsecondary education, including its compliance with Title VI of the Civil Rights Act, campus safety concerns over disruptions to the learning environment, and the awarding of federal student aid under the Higher Education Act.
• The Committee on Oversight and Accountability is investigating the sources of funding and other support flowing to groups espousing pro-Hamas propaganda and engaged in antisemitic harassment and intimidation of students. The Committee on Oversight and Accountability is the principal oversight committee of the US House of Representatives and has broad authority to investigate “any matter” at “any time” under House Rule X.
• The Committee on Ways and Means has been investigating several universities since November 15, 2023, when the Committee held a hearing entitled From Ivory Towers to Dark Corners: Investigating the Nexus Between Antisemitism, Tax-Exempt Universities, and Terror Financing. The Committee followed the hearing with letters to those institutions on January 10, 202
How to Make a Field invisible in Odoo 17Celine George
It is possible to hide or invisible some fields in odoo. Commonly using “invisible” attribute in the field definition to invisible the fields. This slide will show how to make a field invisible in odoo 17.
A Strategic Approach: GenAI in EducationPeter Windle
Artificial Intelligence (AI) technologies such as Generative AI, Image Generators and Large Language Models have had a dramatic impact on teaching, learning and assessment over the past 18 months. The most immediate threat AI posed was to Academic Integrity with Higher Education Institutes (HEIs) focusing their efforts on combating the use of GenAI in assessment. Guidelines were developed for staff and students, policies put in place too. Innovative educators have forged paths in the use of Generative AI for teaching, learning and assessments leading to pockets of transformation springing up across HEIs, often with little or no top-down guidance, support or direction.
This Gasta posits a strategic approach to integrating AI into HEIs to prepare staff, students and the curriculum for an evolving world and workplace. We will highlight the advantages of working with these technologies beyond the realm of teaching, learning and assessment by considering prompt engineering skills, industry impact, curriculum changes, and the need for staff upskilling. In contrast, not engaging strategically with Generative AI poses risks, including falling behind peers, missed opportunities and failing to ensure our graduates remain employable. The rapid evolution of AI technologies necessitates a proactive and strategic approach if we are to remain relevant.
2. Danio rerio constituye un modelo
actualmente indiscutible en biomedicina.
Útil para estudiar efectos tóxicos de
sustancias químicas presentes en el agua,
como modelo para aterosclerosis y otras .
3. “Many researchers and clinicians agree that the
treatment of atherosclerosis must begin at the earliest
possible stage – the fatty streak,” said Miller.
“By feeding HCD to zebrafish, we were able to reproduce
many of the processes involved in early
atherogenesis. Our results suggest that this new model
is suitable for studying inflammatory processes that
occur in the early development of the disease, by looking
at the function of vascular cells and lipid deposits in a
live animal.”
5. Cuidado y Mantenimiento de Zebrafish (Danio
rerio) en el Laboratorio.
Avdesh, A., Chen, M., Martin-Iverson, M. T., Mondal, A., Ong, D., Rainey-Smith, S., … Martins, R. N. (2012).
Regular Care and Maintenance of a Zebrafish (Danio rerio) Laboratory: An Introduction. Journal of
Visualized Experiments : JoVE, (69), 4196. doi:10.3791/4196
http://www.jove.com/video/4196/regular-care-maintenance-zebrafish-danio-rerio-laboratory-an
Hembras
(B y C)
Macho
(A)
6. PARÁMETRO RANGO ÓPTIMO
Alcalinidad 50-150 mg/L CaCO3
pH 6.8-7.5 (6.0-8.5 tolerable)
Temperatura 26 - 28.5 °C
Dureza 50-100 mg/L CaCO3
NH4 (Amonio no iónico) <0.02 mg/L
Nitratos (NO3-) <50 mg/L
Nitritos (NO2-) <0.1 mg/L
Oxígeno disuelto >6.0 mg/L
Salinidad 0.5-1 g/L
Conductividad 300 -1,500 μS
Condiciones del agua.
8. Manejo de Zebrafish (Danio rerio) en el Laboratorio.
Hembra adulta de 4 meses de edad y 49 mm de longitud.
9. Embrión de 24 horas.
-Helenius, I. T., & Yeh, J.-R. J. (2012). Small zebrafish in a big chemical pond.Journal of Cellular
Biochemistry, 113(7), 2208–2216. doi:10.1002/jcb.24120
Desarrollo rápido y visualización sencilla de tejidos y
órganos durante el desarrollo de los embriones.
Células musculares y
estructura de los
miótomos.
Glóbulos rojos y
vasos.
Cardiomiocitos
(verde)
Estructura del
cerebro.
10. Simmons, A. E., Karimi, I., Talwar, M., & Simmons, T. W. (2012). Effects of Nitrite on Development of
Embryos and Early Larval Stages of the Zebrafish (Danio rerio). Zebrafish, 9(4), 200–206.
doi:10.1089/zeb.2012.0746
A: Sin nitritos (0 mg/L)
Efectos de la exposición a nitritos en larvas de
zebrafish.
Vista dorsal de la
larva de 5 días
(120 horas post
fertilización .
B: Expuesta a nitritos
(1500 mg/L durante 24
horas = hpf).
11. Simmons et al., (2012).
A: en 300 mM de etanol
durante 24 horas luego
de la fertilización.
Vista antero-
lateral de la
larva de 5 días.
Teratogénesis
causada por
etanol y nitritos.
B : 300 mg/L de
nitritos durante 96
horas.
12. Helenius, I. T., & Yeh, J.-R. J. (2012). Small zebrafish in a big chemical
pond. Journal of Cellular Biochemistry, 113(7), 2208–2216.
doi:10.1002/jcb.24120
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3349782/
13. Pancreas, Liver, Gut screens
using in situ hybridization.
Fishman, M. C. (1999). Zebrafish genetics: The enigma of arrival. Proceedings of the National Academy of
Sciences of the United States of America, 96(19), 10554–10556.
Genética y zebrafish: visualizacion de los órganos embrionarios “in vivo” .
.
Thymus and neural tube
after green fluorescent
protein transgenesis
(GFP= green fluorescent
protein).
Heart and
Notochord, when
enhanced by
fluorescence
Blood vessel after
injection of fluorescent
dextran to fill the
vascular tree.
Kidney:
Immunohistochemistry.
14. Respuesta angiogénica observada por
microscopía confocal.
Blood vessels of
transgenic fli1a:EGFP zebrafish embryos can be
easily observed under the confocal microscope
(A), and SIVs are marked by white arrows.
Angiogenic responses (B and C) are triggered
by tumor cell xenografts and targeted
antiangiogenic therapy of RGD-SWCNT(Rh)-
thalidomide (D, E and F) in
transgenic fli1a:EGFP zebrafish embryos.
Engraftment of human HT1080 fibrosarcoma
cells, which secrete vascular endothelial growth
factors, triggers ectopic angiogenesis of SIVs
(B and C). Note morphological features of
angiogenic response with engraftment of human
HT1080 fibrosarcoma cells. When coinjected
with RGD-SWCNT(Rh)-thalidomide (E), ectopic
growth of angiogenesis of the SIV is obviously
inhibited (D and F) in treated zebrafish embryos.
White arrows (E and F) indicate presence of
RGD-SWCNT(Rh)-thalidomide after injection.
(F) is the merge of (D) and (E). Scale bar: 200
μm.
15. Zebrafish & Angiogénesis
Cheng, J., Gu, Y.-J., Wang, Y., Cheng, S. H., & Wong, W.-T. (2011). Nanotherapeutics in angiogenesis: synthesis and in
vivo assessment of drug efficacy and biocompatibility in zebrafish embryos. International Journal of Nanomedicine, 6,
2007–2021. doi:10.2147/IJN.S20145
16. Stoletov, K., Fang, L., Choi, S.-H., Hartvigsen, K., Hansen, L. F., Hall, C., …
Miller, Y. I. (2009). Vascular lipid accumulation, lipoprotein oxidation and
macrophage lipid uptake in hypercholesterolemic zebrafish. Circulation
Research, 104(8), 952–960.
“Here we report that feeding adult zebrafish (Danio rerio)
a high-cholesterol diet (HCD) resulted in
hypercholesterolemia, remarkable lipoprotein oxidation
and fatty streak formation in the arteries”.
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2834250
17. Vascular lipid accumulation, lipoprotein oxidation and macrophage lipid uptake in
hypercholesterolemic zebrafish.
E = antibody
against L-
plastin
(macrophages)
counterstained
with DAPI
(nuclei)
A = fatty
streaks in the
dorsal aorta.
Melanocytes
(mln)
accumulate
around blood
vessels.
DA = Dorsal aorta. CV= Caudal vein; ISA = inter-segmental artery bifurcation. mln, melanocytes.
HCD-fed
van Gieson
staining
control
5 μm
20 μm
D and E = DA
stained with
LipidTOX Red
(neutral lipid;
merged
fluorescent
and bright
field images)
18. Zebrafish y Nicotina
Klee, E. W., Ebbert, J. O., Schneider, H., Hurt, R. D., & Ekker, S. C. (2011).
Zebrafish for the Study of the Biological Effects of Nicotine. Nicotine & Tobacco
Research, 13(5), 301–312. doi:10.1093/ntr/ntr010
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3145391/
Kedikian X, Faillace MP, Bernabeu R (2013) Behavioral and Molecular Analysis of
Nicotine-Conditioned Place Preference in Zebrafish. PLoS ONE 8(7): e69453.
doi: 10.1371/journal.pone.0069453
19. Nanotherapeutics in
angiogenesis: synthesis and in
vivo assessment of drug
efficacy and biocompatibility in
zebrafish embryos (Jinping
Cheng, et al., 2011).
Eficacia de la biodistribución,
validación en zebrafish.
In vivo biodistribution of Rh-SWCNT-RGD (rhodamine - Single-Walled Carbon Nanotubes -
Arginylglycylaspartic acid (RGD) in developing zebrafish embryos at different developmental stages.
Zebrafish embryos were loaded with 2 nL of Rh-SWCNT-RGD (2.4 ng of SWCNT and 0.3 ng of RGD) into
embryonic cells at the one-cell stage through microinjection.
Editor's Notes
A 1-day old zebrafish embryo (center) has already developed multiple organ systems, which can be easily observed due to transparency of the embryos and using fluorescent transgenic lines to highlight specific compartments. The effects of small molecules on the morphology or function of multiple tissues can thus be readily detected. Panels: kdrl-GFP and gata1-DsRed show individual DsRed+ red blood cells within GFP+ vessels (top left); mylz2-DsRed shows muscle cells and myotome structure (top middle); membrane-targeted GFP and an mCherry enhancer trap strain show structure of the brain (top right); embryonic heart expressing cmlc2-GFP in cardiomyocytes (bottom left); bright-field image of the embryonic brain ventricle (bottom right).