When using the enzyme coupled reaction for the glucose assay, it is important that the absorbance value determined @415nm has a direct correlation to the amount of glucose in the solution. Glucose + O2 + H2O ----->Glucose Acid + H2O2 H2O2 + ABTS(reduced) [colourless] ------> ABTS+(oxidized) [blue] After reviewing the glucose oxidase/peroxidase-coupled reaction above discuss the following..... a, How would your unknown glucose result be affected if by some chance the enzyme, catalase, was a cintaminant in your unknown glucose example? Explain ur reasoning. b, How would your unknown glucose result be affected if by some chance Vitamin C, was a cintaminant in your unknown glucose example? Explain ur reasoning. Thank you! Solution a) Catalase is an antioxidant that makes the hydrogen peroxide less toxic by converting it into water and oxygen gas. Absence of free hydrogen peroxide to oxidise the ABTS would not give the blue color. b) Vitamin C also serves as antioxidant by reducing the hydrogen peroxide. Therefore, no blue color of the oxidised ABTS would be produced. Hence, in both cases, presence of glucose would be masked by antioxidant activities of the catalase and vitamin C..