This document discusses water treatment for hemodialysis units. It notes that water quality significantly impacts patient outcomes. The summary is as follows:
1. Proper water treatment is essential for hemodialysis patients who are exposed to large volumes of water each week through dialysis.
2. The water treatment system uses various processes like carbon filtration, softening, reverse osmosis, and deionization to remove contaminants.
3. Strict policies, documentation, and staff education are needed to ensure the water treatment system operates safely and provides water that meets quality standards.
The water to be used for the preparation of haemodialysis fluids needs treatment to achieve the appropriate quality. The water treatment is provided by a water pre-treatment system which may include various components such as sediment filters, water softeners, carbon tanks, micro-filters, ultraviolet disinfection units, reverse osmosis units, ultrafilters and storage tanks. The components of the system will be determined by the quality of feed water and the ability of the overall system to produce and maintain appropriate water quality.
During hemodialysis, a hemodialyzer, or artificial kidney, is used to filter fluids and wastes from a dialysis patient's blood. Reuse of a hemodialyzer means that the same hemodialyzer (filter) is used more than once for the same patient. When dialyzers are reused, they are cleaned and disinfected after each treatment.
The water to be used for the preparation of haemodialysis fluids needs treatment to achieve the appropriate quality. The water treatment is provided by a water pre-treatment system which may include various components such as sediment filters, water softeners, carbon tanks, micro-filters, ultraviolet disinfection units, reverse osmosis units, ultrafilters and storage tanks. The components of the system will be determined by the quality of feed water and the ability of the overall system to produce and maintain appropriate water quality.
During hemodialysis, a hemodialyzer, or artificial kidney, is used to filter fluids and wastes from a dialysis patient's blood. Reuse of a hemodialyzer means that the same hemodialyzer (filter) is used more than once for the same patient. When dialyzers are reused, they are cleaned and disinfected after each treatment.
he water to be used for the preparation of haemodialysis fluids needs treatment to achieve the appropriate quality. The water treatment is provided by a water pre-treatment system which may include various components such as sediment filters, water softeners, carbon tanks, micro-filters, ultraviolet disinfection units, reverse osmosis units, ultrafilters and storage tanks. The components of the system will be determined by the quality of feed water and the ability of the overall system to produce and maintain appropriate water quality.
Muscle cramps during hemodialysis by aniqa attaaniqaatta1
title: muscle cramps
this lecture is about muscle cramps which is an cute complication occurs during dialysis, Causes, prevention and management of muscle cramps is given in the lecture
title: headache during dialysis
this lecture is about the headache an acute complication during dialysis. it will cover all the aspects of headache like its prevention management and causes.
he water to be used for the preparation of haemodialysis fluids needs treatment to achieve the appropriate quality. The water treatment is provided by a water pre-treatment system which may include various components such as sediment filters, water softeners, carbon tanks, micro-filters, ultraviolet disinfection units, reverse osmosis units, ultrafilters and storage tanks. The components of the system will be determined by the quality of feed water and the ability of the overall system to produce and maintain appropriate water quality.
Muscle cramps during hemodialysis by aniqa attaaniqaatta1
title: muscle cramps
this lecture is about muscle cramps which is an cute complication occurs during dialysis, Causes, prevention and management of muscle cramps is given in the lecture
title: headache during dialysis
this lecture is about the headache an acute complication during dialysis. it will cover all the aspects of headache like its prevention management and causes.
Presentation given at seminar "Biological nutrient removal, operation management, and troubleshooting at wastewater treatment plant" in Pietari 13.12.2012
Water Services:Introduction
Water treatment
Demineralization
Cold-water services
Hot-water services
Pipe sizing
Materials for water services
water supply sanitation
Drain systems
Prix Galien International 2024 Forum ProgramLevi Shapiro
June 20, 2024, Prix Galien International and Jerusalem Ethics Forum in ROME. Detailed agenda including panels:
- ADVANCES IN CARDIOLOGY: A NEW PARADIGM IS COMING
- WOMEN’S HEALTH: FERTILITY PRESERVATION
- WHAT’S NEW IN THE TREATMENT OF INFECTIOUS,
ONCOLOGICAL AND INFLAMMATORY SKIN DISEASES?
- ARTIFICIAL INTELLIGENCE AND ETHICS
- GENE THERAPY
- BEYOND BORDERS: GLOBAL INITIATIVES FOR DEMOCRATIZING LIFE SCIENCE TECHNOLOGIES AND PROMOTING ACCESS TO HEALTHCARE
- ETHICAL CHALLENGES IN LIFE SCIENCES
- Prix Galien International Awards Ceremony
These simplified slides by Dr. Sidra Arshad present an overview of the non-respiratory functions of the respiratory tract.
Learning objectives:
1. Enlist the non-respiratory functions of the respiratory tract
2. Briefly explain how these functions are carried out
3. Discuss the significance of dead space
4. Differentiate between minute ventilation and alveolar ventilation
5. Describe the cough and sneeze reflexes
Study Resources:
1. Chapter 39, Guyton and Hall Textbook of Medical Physiology, 14th edition
2. Chapter 34, Ganong’s Review of Medical Physiology, 26th edition
3. Chapter 17, Human Physiology by Lauralee Sherwood, 9th edition
4. Non-respiratory functions of the lungs https://academic.oup.com/bjaed/article/13/3/98/278874
Title: Sense of Smell
Presenter: Dr. Faiza, Assistant Professor of Physiology
Qualifications:
MBBS (Best Graduate, AIMC Lahore)
FCPS Physiology
ICMT, CHPE, DHPE (STMU)
MPH (GC University, Faisalabad)
MBA (Virtual University of Pakistan)
Learning Objectives:
Describe the primary categories of smells and the concept of odor blindness.
Explain the structure and location of the olfactory membrane and mucosa, including the types and roles of cells involved in olfaction.
Describe the pathway and mechanisms of olfactory signal transmission from the olfactory receptors to the brain.
Illustrate the biochemical cascade triggered by odorant binding to olfactory receptors, including the role of G-proteins and second messengers in generating an action potential.
Identify different types of olfactory disorders such as anosmia, hyposmia, hyperosmia, and dysosmia, including their potential causes.
Key Topics:
Olfactory Genes:
3% of the human genome accounts for olfactory genes.
400 genes for odorant receptors.
Olfactory Membrane:
Located in the superior part of the nasal cavity.
Medially: Folds downward along the superior septum.
Laterally: Folds over the superior turbinate and upper surface of the middle turbinate.
Total surface area: 5-10 square centimeters.
Olfactory Mucosa:
Olfactory Cells: Bipolar nerve cells derived from the CNS (100 million), with 4-25 olfactory cilia per cell.
Sustentacular Cells: Produce mucus and maintain ionic and molecular environment.
Basal Cells: Replace worn-out olfactory cells with an average lifespan of 1-2 months.
Bowman’s Gland: Secretes mucus.
Stimulation of Olfactory Cells:
Odorant dissolves in mucus and attaches to receptors on olfactory cilia.
Involves a cascade effect through G-proteins and second messengers, leading to depolarization and action potential generation in the olfactory nerve.
Quality of a Good Odorant:
Small (3-20 Carbon atoms), volatile, water-soluble, and lipid-soluble.
Facilitated by odorant-binding proteins in mucus.
Membrane Potential and Action Potential:
Resting membrane potential: -55mV.
Action potential frequency in the olfactory nerve increases with odorant strength.
Adaptation Towards the Sense of Smell:
Rapid adaptation within the first second, with further slow adaptation.
Psychological adaptation greater than receptor adaptation, involving feedback inhibition from the central nervous system.
Primary Sensations of Smell:
Camphoraceous, Musky, Floral, Pepperminty, Ethereal, Pungent, Putrid.
Odor Detection Threshold:
Examples: Hydrogen sulfide (0.0005 ppm), Methyl-mercaptan (0.002 ppm).
Some toxic substances are odorless at lethal concentrations.
Characteristics of Smell:
Odor blindness for single substances due to lack of appropriate receptor protein.
Behavioral and emotional influences of smell.
Transmission of Olfactory Signals:
From olfactory cells to glomeruli in the olfactory bulb, involving lateral inhibition.
Primitive, less old, and new olfactory systems with different path
Explore natural remedies for syphilis treatment in Singapore. Discover alternative therapies, herbal remedies, and lifestyle changes that may complement conventional treatments. Learn about holistic approaches to managing syphilis symptoms and supporting overall health.
NVBDCP.pptx Nation vector borne disease control programSapna Thakur
NVBDCP was launched in 2003-2004 . Vector-Borne Disease: Disease that results from an infection transmitted to humans and other animals by blood-feeding arthropods, such as mosquitoes, ticks, and fleas. Examples of vector-borne diseases include Dengue fever, West Nile Virus, Lyme disease, and malaria.
Acute scrotum is a general term referring to an emergency condition affecting the contents or the wall of the scrotum.
There are a number of conditions that present acutely, predominantly with pain and/or swelling
A careful and detailed history and examination, and in some cases, investigations allow differentiation between these diagnoses. A prompt diagnosis is essential as the patient may require urgent surgical intervention
Testicular torsion refers to twisting of the spermatic cord, causing ischaemia of the testicle.
Testicular torsion results from inadequate fixation of the testis to the tunica vaginalis producing ischemia from reduced arterial inflow and venous outflow obstruction.
The prevalence of testicular torsion in adult patients hospitalized with acute scrotal pain is approximately 25 to 50 percent
263778731218 Abortion Clinic /Pills In Harare ,sisternakatoto
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Ethanol (CH3CH2OH), or beverage alcohol, is a two-carbon alcohol
that is rapidly distributed in the body and brain. Ethanol alters many
neurochemical systems and has rewarding and addictive properties. It
is the oldest recreational drug and likely contributes to more morbidity,
mortality, and public health costs than all illicit drugs combined. The
5th edition of the Diagnostic and Statistical Manual of Mental Disorders
(DSM-5) integrates alcohol abuse and alcohol dependence into a single
disorder called alcohol use disorder (AUD), with mild, moderate,
and severe subclassifications (American Psychiatric Association, 2013).
In the DSM-5, all types of substance abuse and dependence have been
combined into a single substance use disorder (SUD) on a continuum
from mild to severe. A diagnosis of AUD requires that at least two of
the 11 DSM-5 behaviors be present within a 12-month period (mild
AUD: 2–3 criteria; moderate AUD: 4–5 criteria; severe AUD: 6–11 criteria).
The four main behavioral effects of AUD are impaired control over
drinking, negative social consequences, risky use, and altered physiological
effects (tolerance, withdrawal). This chapter presents an overview
of the prevalence and harmful consequences of AUD in the U.S.,
the systemic nature of the disease, neurocircuitry and stages of AUD,
comorbidities, fetal alcohol spectrum disorders, genetic risk factors, and
pharmacotherapies for AUD.
Title: Sense of Taste
Presenter: Dr. Faiza, Assistant Professor of Physiology
Qualifications:
MBBS (Best Graduate, AIMC Lahore)
FCPS Physiology
ICMT, CHPE, DHPE (STMU)
MPH (GC University, Faisalabad)
MBA (Virtual University of Pakistan)
Learning Objectives:
Describe the structure and function of taste buds.
Describe the relationship between the taste threshold and taste index of common substances.
Explain the chemical basis and signal transduction of taste perception for each type of primary taste sensation.
Recognize different abnormalities of taste perception and their causes.
Key Topics:
Significance of Taste Sensation:
Differentiation between pleasant and harmful food
Influence on behavior
Selection of food based on metabolic needs
Receptors of Taste:
Taste buds on the tongue
Influence of sense of smell, texture of food, and pain stimulation (e.g., by pepper)
Primary and Secondary Taste Sensations:
Primary taste sensations: Sweet, Sour, Salty, Bitter, Umami
Chemical basis and signal transduction mechanisms for each taste
Taste Threshold and Index:
Taste threshold values for Sweet (sucrose), Salty (NaCl), Sour (HCl), and Bitter (Quinine)
Taste index relationship: Inversely proportional to taste threshold
Taste Blindness:
Inability to taste certain substances, particularly thiourea compounds
Example: Phenylthiocarbamide
Structure and Function of Taste Buds:
Composition: Epithelial cells, Sustentacular/Supporting cells, Taste cells, Basal cells
Features: Taste pores, Taste hairs/microvilli, and Taste nerve fibers
Location of Taste Buds:
Found in papillae of the tongue (Fungiform, Circumvallate, Foliate)
Also present on the palate, tonsillar pillars, epiglottis, and proximal esophagus
Mechanism of Taste Stimulation:
Interaction of taste substances with receptors on microvilli
Signal transduction pathways for Umami, Sweet, Bitter, Sour, and Salty tastes
Taste Sensitivity and Adaptation:
Decrease in sensitivity with age
Rapid adaptation of taste sensation
Role of Saliva in Taste:
Dissolution of tastants to reach receptors
Washing away the stimulus
Taste Preferences and Aversions:
Mechanisms behind taste preference and aversion
Influence of receptors and neural pathways
Impact of Sensory Nerve Damage:
Degeneration of taste buds if the sensory nerve fiber is cut
Abnormalities of Taste Detection:
Conditions: Ageusia, Hypogeusia, Dysgeusia (parageusia)
Causes: Nerve damage, neurological disorders, infections, poor oral hygiene, adverse drug effects, deficiencies, aging, tobacco use, altered neurotransmitter levels
Neurotransmitters and Taste Threshold:
Effects of serotonin (5-HT) and norepinephrine (NE) on taste sensitivity
Supertasters:
25% of the population with heightened sensitivity to taste, especially bitterness
Increased number of fungiform papillae
Pulmonary Thromboembolism - etilogy, types, medical- Surgical and nursing man...VarunMahajani
Disruption of blood supply to lung alveoli due to blockage of one or more pulmonary blood vessels is called as Pulmonary thromboembolism. In this presentation we will discuss its causes, types and its management in depth.
These lecture slides, by Dr Sidra Arshad, offer a quick overview of physiological basis of a normal electrocardiogram.
Learning objectives:
1. Define an electrocardiogram (ECG) and electrocardiography
2. Describe how dipoles generated by the heart produce the waveforms of the ECG
3. Describe the components of a normal electrocardiogram of a typical bipolar leads (limb II)
4. Differentiate between intervals and segments
5. Enlist some common indications for obtaining an ECG
Study Resources:
1. Chapter 11, Guyton and Hall Textbook of Medical Physiology, 14th edition
2. Chapter 9, Human Physiology - From Cells to Systems, Lauralee Sherwood, 9th edition
3. Chapter 29, Ganong’s Review of Medical Physiology, 26th edition
4. Electrocardiogram, StatPearls - https://www.ncbi.nlm.nih.gov/books/NBK549803/
5. ECG in Medical Practice by ABM Abdullah, 4th edition
6. ECG Basics, http://www.nataliescasebook.com/tag/e-c-g-basics
Lung Cancer: Artificial Intelligence, Synergetics, Complex System Analysis, S...Oleg Kshivets
RESULTS: Overall life span (LS) was 2252.1±1742.5 days and cumulative 5-year survival (5YS) reached 73.2%, 10 years – 64.8%, 20 years – 42.5%. 513 LCP lived more than 5 years (LS=3124.6±1525.6 days), 148 LCP – more than 10 years (LS=5054.4±1504.1 days).199 LCP died because of LC (LS=562.7±374.5 days). 5YS of LCP after bi/lobectomies was significantly superior in comparison with LCP after pneumonectomies (78.1% vs.63.7%, P=0.00001 by log-rank test). AT significantly improved 5YS (66.3% vs. 34.8%) (P=0.00000 by log-rank test) only for LCP with N1-2. Cox modeling displayed that 5YS of LCP significantly depended on: phase transition (PT) early-invasive LC in terms of synergetics, PT N0—N12, cell ratio factors (ratio between cancer cells- CC and blood cells subpopulations), G1-3, histology, glucose, AT, blood cell circuit, prothrombin index, heparin tolerance, recalcification time (P=0.000-0.038). Neural networks, genetic algorithm selection and bootstrap simulation revealed relationships between 5YS and PT early-invasive LC (rank=1), PT N0—N12 (rank=2), thrombocytes/CC (3), erythrocytes/CC (4), eosinophils/CC (5), healthy cells/CC (6), lymphocytes/CC (7), segmented neutrophils/CC (8), stick neutrophils/CC (9), monocytes/CC (10); leucocytes/CC (11). Correct prediction of 5YS was 100% by neural networks computing (area under ROC curve=1.0; error=0.0).
CONCLUSIONS: 5YS of LCP after radical procedures significantly depended on: 1) PT early-invasive cancer; 2) PT N0--N12; 3) cell ratio factors; 4) blood cell circuit; 5) biochemical factors; 6) hemostasis system; 7) AT; 8) LC characteristics; 9) LC cell dynamics; 10) surgery type: lobectomy/pneumonectomy; 11) anthropometric data. Optimal diagnosis and treatment strategies for LC are: 1) screening and early detection of LC; 2) availability of experienced thoracic surgeons because of complexity of radical procedures; 3) aggressive en block surgery and adequate lymph node dissection for completeness; 4) precise prediction; 5) adjuvant chemoimmunoradiotherapy for LCP with unfavorable prognosis.
1. Water treatment for HD Unit
DR Samir Sally, MD
Consultant Internal Medicine & Nephrology,
MUNC, Mansoura University, Egypt
2. Introduction
• Survival of HD patients is steadily improving
• Water treatment for preparation of dialysate is
probably the most neglected area of RRT with
dialysis
• Quality of water contributes very significantly in
acute and long term morbidity and prognosis
3. Introduction
• Dialysis staff should have a fundamental understanding of
water pre-treatment for haemodialysis
• Written policies, practices and procedures shall be in place
for the safe operation of dialysis water pre-treatment systems
• All servicing, maintenance, interventions and changes to the
water pre-treatment plant, as a minimum, shall be recorded
in an on-site log book
4. Introduction
The worst way to hurt patients is through the
water system, so it is expected that you know
what is going on.
5. Exposure to Water and Contaminants
Average Population
• Drinks approximately 14
L/week (2L/day)
• Able to excrete toxic
substances in urine
• Contaminants
selectively absorbed in
GI tract, indirectly
exposed to blood
Hemodialysis Patient
• Exposed to
approximately 360 Lw
• Kidneys unable to
excrete toxic substances
• Contaminants directly
exposed to blood via
dialyzer membrane
7. Water supply
There are 2 sources of municipal water:
• Surface water is generally more contaminated
with organisms and microbes, industrial
wastes, fertilizers, and sewage.
• Ground water is generally lower in organic
materials but contains higher inorganic ions
such as iron, ca, mg and sulfate
8. Toxic effects of water contaminants in HD
Contaminant Possible effects
Aluminum Dialysis encephalopathy, renal bone disease
Calcium, Magnesium Hard water syndrome, hypertension,
hypotension
Chloramine Hemolysis, anemia, methameglobinemia
Copper Nausea, headache, liver damage, fatal hemolysis
Fluoride Osteomalacia, osteoporosis
Sodium Hypertension, pulmonary edema, confusion,
headache, seizures, coma
Microbial Pyrexia reactions, chills, fever, shock
Nitrate Methmeglobinemia, hypotension, nausea
High iron Hemosiderosis
Sulfate Nausea, vomiting, metabolic acidosis
Zinc Anemia, vomiting, fever
Aromatic hydrocarbons Potential chemical carcinogens
9. Progressive Dialysis Encephalopathy from Dialysate
Aluminium
Arch Intern Med V138, 1978
• 8 cases of fatal dialysis encephalopathy
observed in 22 months (38% of all patients)
• Coincided with addition of Al SO4 and Na
aluminate to city water resulting in dialysis
fluid Al concentration of 200-1000 ug/l
• The outbreak ended after installation of
deionizer that reduced dialysis fluid Al to < 1
ug/l
10. • “Philadelphia Incident” of 1987. Initially, a nurse in the facility noticed an
unusually large number of hematocrit values that were lower than normal.
• Forty-four patients out of 107 required transfusions, and 10 were sent to
the emergency department for additional treatment.
• Upon further investigation, The staff person monitoring the system
recorded the chloramine levels accurately as they climbed to toxic levels
(AAMI maximum level is 0.1 mg/L), but the staff member was not aware
that this was a dangerous situation and did not report it to a supervisor.
• Further, no written policy was in place regarding the testing of total
chlorine levelsThis incident illustrates the need for staff education
11. Arnow PM et al. An outbreak of fatal fluoride intoxication in a
long-term hemodialysis unit. Ann Intern Med 1994;121:339-
344.
• On 16 July 1993, 12 patients treated at a long-term HD unit in
Chicago became ill during or soon after HD.
• The patients experienced symptoms of severe pruritus,
headache, nausea, and chest or back pain.
• Three patients arrested and died due to ventricular fibrillation
after completion of dialysis that day.
• Subsequent investigations found that fluoride was released
from the deionization system after the ion exchange resin
inside was exhausted.
• The investigator concluded that the incident was caused by
errors in maintenance of the deionization system
12. • In February 1996, Caruaru,Brazil, 116 (89%) of 131
patients experienced visual disturbances, nausea,
vomiting, and muscle weakness, following routine HD
treatment. Subsequently, 100 patients developed acute
liver failure. As of December 1996, 52 deaths occurred.
• Two groups of hepatotoxic cyanotoxins were idnetified:
microcystins, specifically microcystin-YR, -LR and -AR.
• The outbreak occurred in one of two HD units using same
water source
13. • The senior clinician in charge of the renal unit
(or designated deputy) has responsibility for
the overall clinical governance in respect of
water treatment facilities
14. • Water companies should also advise the renal
unit if there are plans to alter the range of
chemicals added to the water supply to ensure
compliance with the drinking water directive.
15. Bio med
1-Disinfection of water treatment unit and portable RO
2-Daily TDS, conductivity and PH
3-Checking all machines maintenance and contact with the
manufacturing companies
4-Awarness of all AAMI standards
5-Check free chlorine or chloramine test
16. Water treatment system
1.Feed Water Components: (Back-flow preventer, Temperature
blending valve, booster pump and raw water tank, ±acid feed
pump )
2.Pre-treatment section: (filters, activated carbon and softner).
3.Treatment section: (reverse osmosis).
4.Post-treatment section: (microbial and UV filters or/and
deionization)
5.the distribution system: piping, valves, pumps, ±storage tanks
17.
18. Back flow preventer: inhibits flow
back of treated water into
municipal water
Temperature blending valve:
brings water to a standard
temperature of 25 oC for
proper function of RO system
Booster pump: maintains adequate
flow and pressure of water
so the system operates
successfully.
19. Acid feed pump
• By increasing the pH of the city water supply using
lime softening agents or Ca CO3 prevent leaching of
lead, copper from the piping system
• For proper function of RO and carbon tanks,
incoming water pH should be between 5-8.5
• A pH higher than 8.5 with chloramines present will
cause the carbon to be less adsorptive and the RO
membrane performance to degrade, resulting in
poor water quality.
21. Multimedia depth filter
• Large particulates of >10
microns such as dirt, are
removed by a multimedia
depth filter.
• Floculants can clog the carbon
and softener tanks, destroy the
RO pump, and foul the RO
membrane
• Contain multiple layers of
various sized rocks that trap the
large particles as the water
filtered downward
22. Carbon Tank
• Removes chlorine and
chloramine
• These are high level
oxidative chemicals.
They are added to
municipal water
systems to kill bacteria,
but they also cause
hemolysis
23.
24. Carbon filter
•As the input water flows down through the granular activated
carbon (GAC), solutes diffuse from the water into the pores of the
carbon and become attached to the structure.
•A 10-minute exposure time of the water through the carbon
tanks to reduce chlorine to at least 0.5 mg/L and the
chloramineto be adsorbed to at least 0.1 mg/L.
A wide variety of naturally occurring and synthetic organic
compounds, such as herbicides, pesticides, and industrial
solvents, will be adsorbed as well
25. Water Softener
• Water containing Ca and Mg
form deposits on RO
membrane
• Softeners work on ion
exchange basis. The resin
beads within the tank have a
high affinity for the cations Ca
and Mg (divalents) present in
the source water and release
2 sodium ions (monovalent)
for one Ca or Mg captured
26. Hardness of water
• Calcium carbonate Classification
• 0–60 mg/L Soft
• 61–120 mg/L Moderately hard
• 121–180 mg/L Hard
• Greater than 180 mg/L Very hard
27. Water Softener
• The softener needs regenerating on a routine
basis with concentrated NaCl solution (brine)
before the resin capacity is used up
• The resin is backwashed to loosen the media
and clean any particulates from the tank. After
the backwashing step, the brine solution is
drawn into the tank
28.
29. Reverse Osmosis system
• RO overcomes natural osmosis by forcing feed
water under pressure thru a semi-permeable
membrane leaving contaminants behind (ions,
organics)
30. Reverse osmosis
•Reverse osmosis (RO) uses high pressure to force water across a
semipermeable membrane, thereby rejecting
90 to 99 percent of ionic compounds and
>95 percent of nonionic contaminants,
also it is an effective barrier against microbiological contaminants,
including bacteria, viruses, and endotoxin.
31.
32. Ion exchange deionizers (DI)
DI use a two-stage process to remove virtually all ionic material
remaining in pretreated water.
Two types of synthetic resins are used; one to remove positively
charged ions (cations) and another to remove negatively charged
ions (anions).
•Although deionizers produce water of very high purity with
respect to ionic contaminants, they do not remove
microbiological contaminants.
•Therefore, it is necessary to incorporate bacterial control
equipment after the application of a deionizer.
33. Ultraviolet filter
The mechanism of microorganism destruction is currently
believed to be that ultraviolet causes molecular rearrangements
in DNA and RNA, which in turn blocks replication.
•As the UV kills the bacteria, it may increase the level of
endotoxinas a result of the destruction of the gram-negative
bacteria (endotoxin producing) cell wall
35. Distribution system
• RO distribution systems: direct feed and
indirect feed
• Direct feed: directly delivers the product water
from the RO unit to the loop for distribution
• Indirect feed: involves a storage tank that
accumulates the product water and delivers to
the distribution loop
• Unused portions are recirculated back into the
storage tank
36. Microbiological aspects of fluid system
design
• use of a recirculation-type system,
• avoidance of dead ends and dead space areas,
• use of materials compatible with the planned methods of
disinfection
• avoidance of storage tanks. If a storage tank is necessary it
should be cleaned and disinfected.
• The disinfection program could prevent the formation of
biofilm, which can become difficult to eradicate.
37. Ultrapure dialysis solution
• Decreases CRP and IL-6
• Improves response to EPO
• Promotes better nutrition
• Reduces plasma levels of ß-2-microglobulin
• Slows loss of residual renal function
• Lowers cardiovascular morbidity
• Bacteria level below 0.1 cfu/ml and
endotoxin level below 0.03 EU/ml
Susantitaphong P et al. Effect of ultrapure dialysate on markers of inflammation, oxidative stress,
nutrition and anemia parameters: a meta-analysis. NDT (2013) 28: 438-446
38. Water treatment unit
TDS, and conductivity daily
Microbiological culture and endotoxin assay.
Monthly
Chemical assay every 6 months
39. Water treatment unit
• There is no limit for RO product TDS/conductivity.
• Values that are acceptable in one location may not be
acceptable in another location.
• TDS in some areas of 50 ppm is acceptable and other areas
where 10 ppm is NOT acceptable. It all depends on your raw
water.
• A slight change in the amount of Fluoride injected into the
water can cause the RO product water to go less than a 1 ppm
TDS increase.
40. Bacteriological Monitoring:
• The maximum level of bacteria in water used
to prepare dialysis fluid must not exceed the
AAMI standard of 100 CFU. The AAMI action
level is 50 CFU
• An action level is defined as a point when
measures must be taken to correct the
potential source to remain in compliance with
AAMI standards
41. Endotoxin standard
• The maximum level of endotoxin in water
used to prepare dialysis fluid must not exceed
the AAMI standard of 0.25 Endotoxin Units/
ml (EU/ml)
• The action level of endotoxin is 0.125 EU/ml
45. Contaminant Maximum Concentration
mg/L
Test Methodology
Selenium 0.09 Atomic Absorption (gaseous, or
electrothermal)
Silver 0.005 Atomic Absorption (electrothermal)
Aluminum 0.01 Atomic Absorption (electrothermal)
Chloramines 0.10 DPD Ferrous Titrimetric Method
Total chlorine 0.50 DPD Ferrous Titrimetric Method
Copper 0.10 Atomic Absorption (direct aspiration)
Fluoride 0.20 Ion Selective Electrode Method
Nitrate (as N) 2.00 Cadmium Reduction Method
Sulfate 100.00 Turbidimetric Method
Thallium 0.002 Atomic Absorption (platform)
Zinc 0.10 Atomic Absorption (direct aspiration)
Association for the Advancement of Medical Instrumentation. Dialysate for hemodialysis
(ANSI/AAMI RD52:2004). Arlington (VA). American National Standard. 2004
46. Water treatment unit
A programme of improvement should begin
immediately if routine monitoring
demonstrates that concentrations of chemical
contaminants exceed the maximum allowable
limits of AAMI. (1B)
47. Disinfection
General
• Disinfection schedules should be designed to prevent
bacterial proliferation, rather than being designed to
eliminate bacteria once they have proliferated to an
unacceptable level (i.e. above the action level).
• A proper disinfection strategy is to be preventive and
this should be applied from the start of operation.
48. Disinfection
• Disinfection of the distribution piping systems.
• 1- Chemical disinfection
• 2- Hot water disinfection When used to control bacterial
proliferation (minimum distribution loop temp 60 oC).
• Heat disinfection will not remove established biofilms, but is
convenient, requires little rinse time and can thus be used
more often to prevent biofilm formation. An occasional
chemical disinfection might still be necessary.
49. Testing of samples
• Testing of water samples shall be carried out by
trained and accredited persons or accredited
laboratories.
• The dialysis unit shall maintain records of persons
who have been trained and accredited and full
details of accredited laboratories.
• The records shall be maintained within the dialysis
unit.
50. Sample collection
• Water sample sites
• Samples are to be taken at outlets of the water distribution system.
• Prior to sampling, the inside of the outlet can be disinfected, especially if no hemodialysis
machine is attached. The reason for such disinfection is that, over time, residual water in an
outlet will support microbial growth. The disinfection can be made by flushing the inside of
the outlet with 70 % ethanol or iso-propanol. A sterile cotton swab wetted with alcohol can
also be used. Exposure time is to be >15 s.
• It is sufficient to let out enough water to rinse off the alcohol (200 ml to 500 ml) prior to
sampling.
• Alternatively, hoses can be disconnected from the tap and the taps opened and allowed to
flush for 2 min to 3 min before aseptically collecting a sample.
• Sample for cultivation and endotoxin analysis: Sample volume 5 ml to 1,000 ml or volume as
specified by the laboratory..
51. Sample collection
• Dialysis fluid samples
• Dialysis fluid samples should be collected from a sampling port prior to the
dialyzer. The sample port should be designed to minimize the likelihood of
contaminating the sample and should be capable of being effectively disinfected.
• Many dialysis machines are equipped with dialysis fluid sample ports that can be
accessed using a syringe. These sample ports may be disinfected with 70 %
ethanol or iso-propanol and allowed to air dry.
• A sterile syringe (20 ml or larger) should then be used to aspirate dialysis fluid out
of and into the port before filling the syringe. The filled syringe should then be
discarded and a fresh sample of dialysis fluid collected using a new sterile syringe.
• Sample for cultivation and endotoxin analysis: Sample volume 5 ml to 1,000 ml or
a volume as specified by the laboratory. Containers for samples to be cultured
should be sterile and containers for samples to be tested for endotoxins should be
sterile and endotoxin-free.
52. Storage of samples
• Heterotrophic plate count
• Storage of samples
• Microbial analysis of water and dialysis fluid samples should be conducted
as soon as possible after collection to avoid unpredictable changes in the
microbial population. If samples cannot be analyzed within 4 h of
collection, follow the laboratory's instructions for shipping. Samples
intended for colony counts should not be frozen.
• Storage of samples for endotoxin analysis may be different from what is
given above, provided the complete procedure follows the
manufacturer's instructions for use of the LAL assay.
53. Conclusion
• Water treatment is a generally neglected area of dialysis
therapy
• Quality of water contributes very significantly in acute and
long term morbidity and prognosis
• Due to increased survival of dialysis patients, increased use of
bicarbonate dialysate and high flux membranes, water
treatment has become essential
• It is worthwhile achieving the goal of sterile, pyrogen free and
chemically pure water for dialysis
54. Conclusion
• Written policies, practices and procedures shall be in place
• AAMI standards are the accepted minimum standards for
water pre-treatment for haemodialysis.
Each unit should have standard operating procedures in place
for sampling, monitoring and recording of feed and product
water quality
• All servicing, maintenance, interventions and changes to the
water pre-treatment plant shall be recorded.
.
55. Conclusion
• Medical directors who learn, know, and embrace the
requirements for providing high-quality dialysis
water will be most successful in this task.
• Medical, nursing and technical staff working in
dialysis units share responsibility for the safe
operation of the water pre-treatment plant and shall
participate together in regular multidisciplinary
committee meetings to review the safe operation of
the water pre-treatment plant and RO water plant.