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Vibrio parahaemolyticus
LOKESHWARI.B
1ST M.SC MICROBIOLOGY,
SACRED HEART COLLEGE (AUTONOMOUS)
TIRUPATTUR.
GENERAL CHARACTERISTICS
 Gram’s classification - Gram-negative Bacteria
 Shape - Curve rod-shaped Bacilli
 Spores - Non-spore forming Bacteria
 Motility – Motile (Swimming Motility)
 Size – 0.5 – 1 µm in diameter
 Family – Vibrionaceae
 Capsule – Present (Occasionally)
GENERAL CHARACTERISTICS
 Endospore – Absent
 Respiration – Facultative anaerobic
 Optimum temperature - 30 to 35°C
 Optimum pH - 6.8 to 10.2
 Habitat – Ubiquitous marine bacterium
 Mainly found in the free – living sea water and marine
environments.
 Organisms are Halophilic (Salt- loving)
 Discovered – Tsunesaburo Fujino in 1950
SCIENTIFIC CLASSIFICATION
Domain – Bacteria
Family – Vibrionaceae
Order – Vibrionales
Phylum – Pseudomonodota
Scientific name – Vibrio
parahaemolyticus
PATHOGENICITY
DISEASE
TRANSMISSION
Consuming raw
and under-cooled
fish / shell fish.
Adheres to living
surfaces (Aquatic
animals)
Open wounds.
INCUBATIONPERIOD
4-30 hours (usually12-24
hours).
GENERATION PERIOD
10-11 Minutes.
VIRULENCE FACTORS
STRUCTURALCOMPONENTS;
Capsules
Phosphatases
Lipopolysaccharides
Cholera toxin
Streptolysin
Phospholipase
Coagulase
TOXINS : THERMO STABLE DIRECT HEMOLYSIN (TDH)
TDH -RELATED HEMOLYSIN (TRH).
TOXINS
(TDH) (TRH).
ENZYMES
Gelatinase
(GEL)
Lipase
(LIP)
Amylase
(AML)
Dn-ase.
SOURCE OF
INFECTION
Shell fish
Sea water
MODE OF
TRANSMISSION
Ingestion of
contaminated
water (sea
foods)
Vibrio parahaemolyticus
Vibrio – quiver
Para – side of
Haema – blood
Lyticus – dissolving
blood
PATHOGENESIS
Infection usually occurs through the fecal-oral route and
adheres to the host cell
Thermostable direct hemolysin (tdh) causes lysis of
human erythrocyte cells
Vibrio parahaemolyticus also forms a toxin for its
enzymatic activity
Tdh is responsible for causing toxicity of cells
As the osmotic pressure increases in the cell its morphology
and self regulation will expand and eventually die.
DETECTION METHODS
Molecular
techniques
Biochemical
tests
PCR – Based
assays
DNA – Based
methods
LAMP
RAPD –
PCR
SIGNS AND SYMPTOMS
1. Watery diarrhea
1. Watery diarrhea
2. Vomiting
3. Nausea
4. Abdominal cramps
5. Fever
6. Chills.
7. Mucus or blood in stool
8. Death
CLINICAL DISEASES
Gastro-
enteritis
Wound
infection
Bacteremia
CLINICAL DISEASES
1. Gastro-enteritis
• Digestive problem
• Lasts for 2 days
• Treated with antibiotics
3.Woundinfection
• Bacteria enter and
multiplies inside
wounds
2. Bacteremia
• Present in the
blood
EPIDEMIOLOGY
1.
2.
3.
4.
5.
6.
LABAROTARY DIAGONOSIS
MICROSCOPIC EXAMINATION
• Gram staining – Pink coloured Gram negative Bacilli.
• Motility Test – Motile
LABAROTARY DIAGONOSIS
COLONY MORPHOLOGY
ON CULTURE MEDIUM
Blood agar– Beta hemolysis
Mac conkey agar – Pink colonies
SELECTIVE MEDIA
TCBS agar – Blue green colonies
BIO CHEMICAL TESTS
Catalase test- Positive (Bubbles formation)
Oxidase test – Positive ( Blue)
Urease test- Positive (Pink)
Indole test – Positive (Pinkish red)
Methyl red test – Positive (Red)
Voges proskauer test – Negative (Pinkish red)
Nitrate reduction test – Positive (Pinkish red)
H2s production – Negative ( Black)
Triple sugar iron test – Positive ( Yellow butt and red slant)
PREVENTION AND CONTROL
Maintains
personal
hygiene
Avoiding
cross-
contaminatio
n between
raw and
cooked
shellfish
Do not get
exposed to
saltwater or
brackish
water
Avoid using
seawater for
cooking.
Avoid
consumption
of raw or
undercooked
seafood
TREATMENT
Disease is
self-
limiting.
Oral-
Rehydration
Drinking
plenty of
fluid
Bed rest Antibiotics
THANK YOU

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Vibrio parahaemolyticus (Gram negative bacteria)