18. Implication of the results
• Better patient acceptance
• No Costly Clinical setup required
• No requirement of Dedicated Manpower and training for sample collection
Pap smear, LBC, VIA, Cervical Washings for HPV
Following needs to sorted out first
• Low urinary excretion
• Sample timing - Morning sample Vs First Stream
• Need for preservatives - Yes/No
• Standardization DNA extraction Procedure
• Standardization of DNA Amplification Procedure
19. Take Home Message and Way forward
Hr-HPV detection in Urine for cervical cancer screening can be a
reasonable alternative to Cervical washing
• Presently recommended for research settings only
• Monitoring of results for mass vaccination can be done by Hr-HPV
detection in urine
• Unmarried or Women unwilling for Vaginal examination may be
offered Urinary Hr-HPV detection for cervical cancer detection
20. Team Members AIIMS Bhopal
Dr Shashank Purwar
Dr Julie Hansa John
Dr Shipra Gupta
Dr Priyal Gupta
Dr Ajay Halder
21.
22.
23.
24. Objectives
1) To assess the percentage concordance (kappa coefficient) between urinary hrHPV (16,18) and histological finding of
cancerous and precancerous lesions of cervix.
1) To find out the likelihood ratio of urinary hrHPV (16,18) positivity among normal and abnormal histopathology
samples.
1) To find out the likelihood ratio of urinary hrHPV (16,18) negativity among normal and abnormal histopathology
samples.
1) To find out the predictive value (positive predictive value and negative predictive value) of urinary hrHPV DNA
(16,18) in the detection of pre-invasive lesions of cervix (abnormal histopathology on cervical biopsy)
28. To study the efficacy of urinary detection of hrHPV
DNA (HPV 16,18) in predicting cervical cancer
(squamous cell carcinoma andadenocarcinoma)
and pre-invasive lesions (CIN-2,3) among women
attending colposcopy clinic in AIIMS Bhopal.”
• ICMR funded research undertaken at
• All India Institute of Medical Sciences Bhopal
