This study evaluated the frequency and distribution of HIV/AIDS cases in Vlora, Albania from September 2012 to 2013. A total of 28 HIV/AIDS cases were reported from 1994 to 2013 in Vlora, with the highest frequency (7 cases) occurring in 2012. In 2012, 6 of the 7 cases were male and 5 were infected through heterosexual transmission. From 2012-2013, 10 total cases were reported with the majority (71%) transmitting through heterosexual contact and 29% transmitting vertically. The study concluded HIV infection rates have increased in the Balkan region and surveillance and prevention programs need strengthening, especially for at-risk groups.