This document discusses a flavonoid complex blend that was found to strongly inhibit the growth of human breast cancer cells in a study. It also discusses several superfruit polyphenols like pomegranate, acai, and green tea that provide benefits based on ancient wisdom and are backed by modern scientific research on cardiovascular, bone, joint, cognitive and other health effects. The document promotes a proprietary product called Tré that provides these polyphenols at standardized, high potency levels from multiple fruits for maximum synergistic benefits.

1. GNLD
International

2. GNLD Business Plan
JOHN R. MILLER
Vice President,
Science & Technology
SAB Member

3. Ancient Wisdom
Modern Science
Timeless Benefits

4. NEW FRONTIERS IN
HEALTH AND LONGEVITY
THE WORLD LEADER
FLAVONOID COMPLEX
Research abstract as published in the FASEB (Federation of American
Societies for Experimental Biology) Journal - 1998
ANTIPROLIFERATIVE EFFECT OF A FLAVONOID RICH FRUIT AND
VEGETABLE BLEND ON HUMAN BREAST CANCER CELLS. A. Carughi.
Background: The prevention of cancer by administration of food phytochemicals is
currently regarded as a promising avenue for cancer control. Epidemiological studies
have demonstrated the relationship between carcinogenesis and diet. Studies on animal
models have also demonstrated that experimentally induced cancer is preventable with
certain natural compounds in the diet. Many flavonoids have been shown to have potent
anticarcinogenic and antitumor activity. For example, quercetin inhibits carcinogenesis
in a number of models and is able to selectively inhibit a variety of tumor cell growth.
Polymethoxylated citrus flavonoids have been shown to have anti-invasive and anti-
metastatic activities. Purpose: To study the antiproliferative effect of a flavonoid rich
fruit and vegetable blend (Flavonoid Complex Active Blend) on human mammary cancer
cells (MCF7). Method: The polyphenol content of the blend was mg/g (measures
as gallic acid equivalents) and contained mg/g quercetin, mg/g kaempherol,
mg/g epicatechin mg/gm hesperidin, mg/g naringenin and mg/g myricetin.
MCF7 (mammary adenocarcinoma cells) were harvested in RPMI 1640 media and
seeded in 96 well plates. A polar and a non polar extract was prepared using 1:1
dichloromethane-methanol and 70% methanol respectively. The extracts were dissolved
in DMSO, combined and serially diluted in the media. The cells were treated and
incubated and for 3 days. Viable cells were quantified using the neutral Red Dye Uptake.
Doxorubicin was used as the positive control. Results: There was a linear response on
percent inhibition (Figure 1). The concentrations used for regression were 150 to 200
ug/ml (r2 = 0.90). Percent inhibition at these points were 41.5 and 82.1 respectively.
Conclusion: This study demonstrates that a flavonoid rich blend of fruit and vegetable
extracts strongly inhibits growth of human breast cancer cells.

5. NEW FRONTIERS IN
HEALTH AND LONGEVITY
Oxidation

6. NEW FRONTIERS IN
HEALTH AND LONGEVITY
Inflammation Oxidation
Evil Twins of Aging & Disease

7. “SUPER FRUIT” POLYPHENOLS

8. WHAT SCIENCE TELLS US…
Polyphenol
Risk
Risk
Polyphenol
Low Polyphenol Diet High Polyphenol Diet

9. NEW FRONTIERS IN
HEALTH AND LONGEVITY
Inflammation Oxidation
Evil Twins of Aging & Disease

10. NEW FRONTIERS IN
HEALTH AND LONGEVITY
 Nutrition for the Brain
 Protection for the Mind
 Sharpness for Today
 Clarity for Tomorrow

11. COGNITIVE LONGEVITY
THE NEW FRONTIER

12. NEW FRONTIERS IN
HEALTH AND LONGEVITY
Mental Clarity & Cognitive Longevity
Heart and Circulatory Health
Cellular Health & Vitality
Joint Flexibility and Function
Anti-Inflammatory Anti-Oxidant
Anti-Aging

13. Ancient Wisdom
Modern Science
Timeless Benefits

14. BASED IN NATURE,
BACKED BY SCIENCE!

15. NEW FRONTIERS IN
HEALTH AND LONGEVITY

16. POMEGRANATE
 Ancient Wisdom
 Fruit of the Gods
 Birth & Eternal Life
 Modern Science
 Cardiovascular
 Bone & Joint
 Prostate / Breast
 Cognitive

17. AÇAI
 Ancient Wisdom
 Tree of Life
 Strength & Energy
 Healing
 Modern Science
 Antioxidant
 Cell protector

18. GREEN TEA
 Ancient Wisdom
 Medicine of the Orient
 Energy & Alertness
 Clarity & Wisdom
 Modern Science
 Cognitive Longevity
 Cell Protection

19. “INFLOX” BERRY BLEND
Blueberry Black Currant
Modern Science
Elderberry Red Grape  Antioxidant
Anti-inflammatory
 Anti-Aging
Bilberry White Grape
 Cognitive protectors
Cranberry

20. HIGH-POTENCY ANTIOXIDANTS
RESVERATROL
ALPHA-LIPOIC ACID (ALA)

21. AGAVE
 Naturally sweetened
with the nectar of the
Agave cactus

22. THE POWER OF DIVERSITY
 Powerful polyphenol
protection
 Nature’s most potent
sources
 Individual effects
 Synergistic benefits

23. THE BENEFITS OF DENSITY
 Standardized potencies
 Assured benefit
 Every time

24. THE VALUE OF TRÉ

25. THE TRÉ ADVANTAGE
M
•Diversity
Total phenolics 110.3 mg/oz 44.4 mg/oz
Total polyphenols 82.3 mg/oz ?
•Density
Standardized amounts of:
No
Punicalagins Yes
Resveratrol Yes No
Alpha-lipoic acid Yes No

26. WORLD CLASS PACKAGING

27. Ancient Wisdom
Modern Science
Timeless Benefits