This document discusses the role of transcription factors (TFs) in regulating gene expression. It provides definitions for general TFs, which are required for basal transcription, and regulatory TFs, which influence the rate of transcription of nearby genes. It describes how TFs contain different domains that allow them to bind DNA and recruit other proteins to regulate transcription. Several examples are given of specific TFs, such as TEOSINTE BRANCHED1, that played important roles in plant domestication by regulating growth. The document also reviews literature on various TFs that confer abiotic stress tolerance in plants and regulate processes like flavonoid biosynthesis.
Signal transduction Calcium Signaling vibhakhanna1
A wide range of Ca2+ signaling pathways deliver the spatial and temporal Ca2+ signals necessary to control the specific functions of different cell types, via various effector proteins and protein kinases
Transcription factors are modular in structure means constructed with standardized units or dimension allowing flexibility and variety in use. It contain 3 domains as ;
1. DNA binding domain – This domain binds to responsive elements present in target DNA. It is conserved region of structure.
2. Trans-activation domain – This domain contain binding site for another proteins ( repressor , activator) . This domains do not generate identifiable electron density in the crystallography analysis , which indicates that thay do not form discreate structures and acts as induced fit model
3. Signal sensing domain - Sense external signals and transmits these to rest of transcription complex
Regulation of gene expression in eukaryotesSuchittaU
This document summarizes gene regulation in eukaryotes. It discusses how gene expression is regulated at multiple levels, including transcription, RNA processing, and intracellular/intercellular signaling. Key points include: (1) Gene expression is controlled by transcription factors binding to promoter and enhancer regions; (2) Eukaryotic gene expression involves RNA splicing and alternative splicing of exons; and (3) The Britten-Davidson model proposes that sensor genes control integrator genes which regulate sets of producer genes in response to signals.
The biosynthesis of the main auxin in plants (indole-3-acetic acid [IAA]) has been elucidated recently and is thought to involve the sequential conversion of Trp to indole-3-pyruvic acid to IAA. However, the pathway leading to a less well studied auxin, phenylacetic acid (PAA), remains unclear. Here, we present evidence from metabolism experiments that PAA is synthesized from the amino acid Phe, via phenylpyruvate. In pea (Pisum sativum), the reverse reaction, phenylpyruvate to Phe, is also demonstrated. However, despite similarities between the pathways leading to IAA and PAA, evidence from mutants in pea and maize (Zea mays) indicate that IAA biosynthetic enzymes are not the main enzymes for PAA biosynthesis. Instead, we identified a putative aromatic aminotransferase (PsArAT) from pea that may function in the PAA synthesis pathway.
Gibberellins are tetracyclic diterpenoid plant hormones that were first discovered in Japan when investigating a fungus that caused abnormal growth in rice plants. There are currently 136 identified gibberellins derived from plants, fungi, and bacteria. Gibberellins promote stem elongation, seed germination, and flower induction. They are synthesized in the leaves and transported to other parts of the plant where they stimulate growth and developmental processes.
Transcription factors and their role in plant disease resistanceSachin Bhor
Transcription factors play an important role in regulating plant gene expression and disease resistance. GhWRKY15 is a transcription factor that has been shown to enhance resistance to viruses and fungi in tobacco plants. Overexpression of GhWRKY15 in tobacco increased the expression of pathogenesis-related genes and activated antioxidant enzymes, reducing reactive oxygen species accumulation. GhWRKY15 was also found to affect plant growth and development, with transgenic tobacco plants exhibiting altered stem morphology and accelerated flowering. The presentation discussed the structure, mechanisms and important families of transcription factors in plants, using GhWRKY15 as a case study to illustrate how transcription factors regulate stress responses.
This document discusses breeding for resistance to abiotic stresses like drought, salt, and cold in fruit crops. It provides information on the characteristics, effects, and mechanisms of different abiotic stresses. It also outlines strategies for breeding resistance, including selecting from cultivated varieties, landraces, and wild relatives. The key mechanisms of resistance include avoidance, tolerance, and acclimation. Traits like early maturity, reduced transpiration, and accumulating osmolytes can provide drought and salt resistance.
Signal transduction Calcium Signaling vibhakhanna1
A wide range of Ca2+ signaling pathways deliver the spatial and temporal Ca2+ signals necessary to control the specific functions of different cell types, via various effector proteins and protein kinases
Transcription factors are modular in structure means constructed with standardized units or dimension allowing flexibility and variety in use. It contain 3 domains as ;
1. DNA binding domain – This domain binds to responsive elements present in target DNA. It is conserved region of structure.
2. Trans-activation domain – This domain contain binding site for another proteins ( repressor , activator) . This domains do not generate identifiable electron density in the crystallography analysis , which indicates that thay do not form discreate structures and acts as induced fit model
3. Signal sensing domain - Sense external signals and transmits these to rest of transcription complex
Regulation of gene expression in eukaryotesSuchittaU
This document summarizes gene regulation in eukaryotes. It discusses how gene expression is regulated at multiple levels, including transcription, RNA processing, and intracellular/intercellular signaling. Key points include: (1) Gene expression is controlled by transcription factors binding to promoter and enhancer regions; (2) Eukaryotic gene expression involves RNA splicing and alternative splicing of exons; and (3) The Britten-Davidson model proposes that sensor genes control integrator genes which regulate sets of producer genes in response to signals.
The biosynthesis of the main auxin in plants (indole-3-acetic acid [IAA]) has been elucidated recently and is thought to involve the sequential conversion of Trp to indole-3-pyruvic acid to IAA. However, the pathway leading to a less well studied auxin, phenylacetic acid (PAA), remains unclear. Here, we present evidence from metabolism experiments that PAA is synthesized from the amino acid Phe, via phenylpyruvate. In pea (Pisum sativum), the reverse reaction, phenylpyruvate to Phe, is also demonstrated. However, despite similarities between the pathways leading to IAA and PAA, evidence from mutants in pea and maize (Zea mays) indicate that IAA biosynthetic enzymes are not the main enzymes for PAA biosynthesis. Instead, we identified a putative aromatic aminotransferase (PsArAT) from pea that may function in the PAA synthesis pathway.
Gibberellins are tetracyclic diterpenoid plant hormones that were first discovered in Japan when investigating a fungus that caused abnormal growth in rice plants. There are currently 136 identified gibberellins derived from plants, fungi, and bacteria. Gibberellins promote stem elongation, seed germination, and flower induction. They are synthesized in the leaves and transported to other parts of the plant where they stimulate growth and developmental processes.
Transcription factors and their role in plant disease resistanceSachin Bhor
Transcription factors play an important role in regulating plant gene expression and disease resistance. GhWRKY15 is a transcription factor that has been shown to enhance resistance to viruses and fungi in tobacco plants. Overexpression of GhWRKY15 in tobacco increased the expression of pathogenesis-related genes and activated antioxidant enzymes, reducing reactive oxygen species accumulation. GhWRKY15 was also found to affect plant growth and development, with transgenic tobacco plants exhibiting altered stem morphology and accelerated flowering. The presentation discussed the structure, mechanisms and important families of transcription factors in plants, using GhWRKY15 as a case study to illustrate how transcription factors regulate stress responses.
This document discusses breeding for resistance to abiotic stresses like drought, salt, and cold in fruit crops. It provides information on the characteristics, effects, and mechanisms of different abiotic stresses. It also outlines strategies for breeding resistance, including selecting from cultivated varieties, landraces, and wild relatives. The key mechanisms of resistance include avoidance, tolerance, and acclimation. Traits like early maturity, reduced transpiration, and accumulating osmolytes can provide drought and salt resistance.
Gene expression in eukaryotes is controlled at multiple levels, including chromatin structure, transcription, RNA processing, and translation. Chromatin structure determines if genes are transcriptionally active or inactive. Transcription is regulated by the interaction of promoters, transcription factors, and enhancers. RNA processing controls splicing and transport of mRNA. Finally, translation and post-translational modifications further regulate gene expression. Overall, eukaryotic gene expression is tightly controlled through complex mechanisms at the chromatin, transcription, RNA, translation, and protein levels.
Brassinosteroids are a class of plant steroid hormones that were first discovered in rapeseed pollen in the 1960s. They influence many developmental processes similar to auxins. The most common brassinosteroid is brassinolide, which was first isolated from rapeseed in 1979. Brassinosteroids regulate processes like cell elongation, flowering, vascular development, photomorphogenesis, and stress tolerance. They are perceived by membrane receptors and signal through a phosphorylation cascade to regulate gene expression.
This document summarizes information about the plant growth hormone auxin. It defines auxin as chemical substances that promote stem or root growth. The first known auxin is indole-3-acetic acid. There are two types of auxin: natural auxins produced by plants like IAA, and synthetic auxins created in labs. The document then describes the three step biosynthesis process of IAA from the amino acid tryptophan. Finally, it lists several physiological effects of auxins, including cell elongation, promotion of cell division, root growth, apical dominance, prevention of abscission, formation of seedless fruits, and regulation of plant growth movements.
the presentation encompasses auxin synthesis, conjugation, degradation, polar and lateral transport and signalling and how all of these together have a bearing on programming and design of the whole plant
1) Eukaryotic gene expression is regulated at multiple levels including transcription, chromatin structure, post-transcriptional processing, and translation.
2) Regulation allows for adaptation and tissue-specific gene expression during development. Key differences from prokaryotes include the lack of operons and more complex regulation in eukaryotes.
3) Gene expression can be regulated short-term through transcriptional control, as seen in yeast galactose-utilizing genes, or long-term for development through mechanisms like chromatin remodeling.
Drought tolerance in plants involves three main mechanisms: morphological, physiological, and genetic/molecular. Morphological mechanisms include drought escape and avoidance strategies like early reproduction or reduced water loss through waxy leaves. Physiological mechanisms regulate water use and loss, like stomatal closure and osmotic adjustment. Genetic and molecular mechanisms change gene expression, upregulating genes that produce proteins protecting cells from stress and regulating hormone signaling and transcription factors that control stress response pathways. Together these overlapping mechanisms help plants adapt and survive periods of low water availability.
RNAi – Mechanism and Its Application In Crop Improvementkundan Jadhao
This document summarizes an RNAi presentation on crop improvement using RNA interference. The 3-sentence summary is:
RNA interference (RNAi) is a process of post-transcriptional gene silencing mediated by small RNA molecules. The presentation described the RNAi pathway and various applications of RNAi technology in crop improvement, including increasing nutrient levels, developing virus and pest resistance, and reducing anti-nutritional compounds. Several case studies were provided that demonstrated how RNAi has been used to successfully modify traits in different crops like maize, cotton, coffee, and banana.
This document provides an overview of flower development. It begins with an introduction to the structure and anatomy of flowers. It then discusses the signals and environmental factors that trigger flowering, including photoperiodism. The document outlines the genetic control of flowering and describes several models of floral organ development, including the ABC hypothesis model. It provides details on the genes responsible for development in each floral whorl based on the ABC model and examples of mutations affecting these genes.
Brassinosteroids are a class of plant hormones that promote growth and development. Over 70 types of brassinosteroids have been isolated from various plant species, including angiosperms, gymnosperms, pteridophytes, bryophytes and algae. They are found in plant tissues and organs such as shoots, seeds, pollen and grains. Brassinosteroids have been shown to increase crop yields, enhance resistance to stresses, and improve plant growth. They are being commercialized for use in agriculture to boost productivity of important crops.
Cellular signal transduction pathways under abiotic stressSenthil Natesan
Abiotic stresses, especially cold, salinity and drought, are the primary causes of crop loss worldwide. Plant adaptation to environmental stresses is dependent upon the activation of cascades of molecular networks involved in stress perception, signal transduction, and the expression of specific stress-related genes and metabolites. Plants have stress-specific adaptive responses as well as responses which protect the plants from more than one environmental stress. There are multiple stress perception and signaling pathways, some of which are specific, but others may cross-talk at various steps (Knight & knight ,2001).Many cold induced pathways are activated to protect plants from deleterious effects of cold stress, but till date, most studied pathway is ICE-CBF-COR signaling pathway (Miura and Furumoto,2013 ) . The Salt-Overly-Sensitive (SOS) pathway, identified through isolation and study of the sos1, sos2, and sos3 mutants, is essential for maintaining favorable ion ratios in the cytoplasm and for tolerance of salt stress (shi .et al ,2002). Both ABA-dependent and -independent signaling pathways appear to be involved in osmotic stress tolerance (Nakashima and shinozaki, 2013) .ROS play a dual role in the response of plants to abiotic stresses functioning as toxic by-products of stress metabolism, as well as important signal transduction molecules and the ROS signaling networks can control growth, development, and stress response ( Mahajan,s and Tuteja, 2005) .
This document summarizes information about Arabidopsis thaliana, a small flowering plant that is widely used as a model organism. It describes A. thaliana's physical characteristics and life cycle, genome, and use in understanding flower development. Specifically, it outlines the ABC model of flower development in which three classes of genes (A, B, and C) interact to specify the four types of floral organs - sepals, petals, stamens, and carpels. Mutations in these genes result in homeotic transformations where one organ develops in place of another.
This document discusses Arabidopsis thaliana and its use in molecular biology research. Some key points:
- A. thaliana is well-suited for genetic research due to its small size, short life cycle, and large seed production. It was the first plant genome sequenced.
- Its genome of about 135 Mbp is among the smallest for higher plants. It contains 5 chromosomes useful for genetic mapping and sequencing.
- The document discusses forward and reverse genetics techniques used to study gene function in A. thaliana such as mutagenesis, screening mutants, positional cloning, and RNA interference. It provides examples of how these approaches have furthered understanding of plant genes and processes.
Major plant hormones include auxins, cytokinins, gibberellins, ethylene, and abscisic acid. Auxins promote cell elongation and root formation, inhibit lateral bud growth, and allow differential growth responses through areas of faster cell elongation. Cytokinins promote cell division and lateral bud growth. Gibberellins promote stem elongation and seed germination. Ethylene inhibits cell expansion and accelerates senescence and fruit ripening. Abscisic acid promotes stomatal closure and inhibits seed germination.
RNAi is a powerful, conserved biological process through which the small, double-stranded RNAs specifically silence the expression of homologous genes, largely through degradation of their cognate mRNA.
Programmed cell death in plants by shivanand b. koppadShivanand Koppad
This document discusses programmed cell death (PCD) in plants. It provides definitions of PCD and necrosis, and describes the differences between the two processes. PCD, also called apoptosis, is an actively controlled and genetically regulated process while necrosis is unregulated cell death in response to external stressors. The document outlines the history of studying PCD/apoptosis and discusses PCD pathways, regulators like caspases, and importance in plant development and response to the environment. It also provides a case study on PCD in tomato fruit in response to heat stress.
This document discusses plant biotechnology and how plants respond to biotic and abiotic stress. It defines homeostasis, stress, and the different types of stresses plants face including biotic (weeds, pathogens, insects) and abiotic (water, temperature, salt, air pollution). It describes how plants can respond to stress through resistance, avoidance, tolerance, or senescence/death. It provides examples of how plants respond to different abiotic stresses like drought, high temperatures, and salt. It also discusses the strategies pathogens use and the physical and induced defenses plants deploy against biotic stresses.
This document discusses regulation of gene expression in eukaryotes. It describes six main levels of control: transcription, RNA processing, mRNA transport, mRNA translation, mRNA degradation, and protein degradation. Key differences between prokaryotic and eukaryotic gene expression are explained, such as eukaryotes possessing nuclei and more complex regulation. Examples of short-term regulation including the GAL gene pathway in yeast and hormone response are provided.
The document discusses signal transduction, which is the process by which extracellular signals are converted into intracellular responses. There are six main steps: 1) synthesis and release of signaling molecules, 2) transport to target cell, 3) detection by receptor, 4) change in cell function triggered by receptor-signal complex, 5) removal of signal, and 6) termination of response. Signal transduction involves cell surface receptors and intracellular receptors that bind ligands and mediate specific cellular responses. Major types of signaling include endocrine, paracrine, and autocrine signaling.
Genetic engineering can be used to induce male sterility in plants by expressing genes that disrupt pollen development. Researchers have successfully transformed tobacco and oilseed rape plants with the barnase gene, which encodes an RNAse enzyme that destroys tapetal cells, preventing pollen formation. Restoration of fertility was achieved by co-expressing the barstar gene, which inhibits barnase. Similarly, expressing the argE gene in rice under a pollen-specific promoter induces male sterility when activated by an inducer, allowing hybrid seed production. Genetic engineering offers possibilities for more efficient hybrid seed systems in crops where traditional methods have not generated usable male sterility.
The document summarizes gene expression in eukaryotes. It discusses that gene expression involves transcription of DNA into mRNA which is then translated into proteins. It describes the key components of transcription including promoters, transcription factors, RNA polymerase, and the formation of the preinitiation complex. It also discusses mechanisms of regulating transcription, such as chromatin remodeling, transcription factors, enhancers, and transcriptional activators.
Gene expression in eukaryotes is controlled at multiple levels, including chromatin structure, transcription, RNA processing, and translation. Chromatin structure determines if genes are transcriptionally active or inactive. Transcription is regulated by the interaction of promoters, transcription factors, and enhancers. RNA processing controls splicing and transport of mRNA. Finally, translation and post-translational modifications further regulate gene expression. Overall, eukaryotic gene expression is tightly controlled through complex mechanisms at the chromatin, transcription, RNA, translation, and protein levels.
Brassinosteroids are a class of plant steroid hormones that were first discovered in rapeseed pollen in the 1960s. They influence many developmental processes similar to auxins. The most common brassinosteroid is brassinolide, which was first isolated from rapeseed in 1979. Brassinosteroids regulate processes like cell elongation, flowering, vascular development, photomorphogenesis, and stress tolerance. They are perceived by membrane receptors and signal through a phosphorylation cascade to regulate gene expression.
This document summarizes information about the plant growth hormone auxin. It defines auxin as chemical substances that promote stem or root growth. The first known auxin is indole-3-acetic acid. There are two types of auxin: natural auxins produced by plants like IAA, and synthetic auxins created in labs. The document then describes the three step biosynthesis process of IAA from the amino acid tryptophan. Finally, it lists several physiological effects of auxins, including cell elongation, promotion of cell division, root growth, apical dominance, prevention of abscission, formation of seedless fruits, and regulation of plant growth movements.
the presentation encompasses auxin synthesis, conjugation, degradation, polar and lateral transport and signalling and how all of these together have a bearing on programming and design of the whole plant
1) Eukaryotic gene expression is regulated at multiple levels including transcription, chromatin structure, post-transcriptional processing, and translation.
2) Regulation allows for adaptation and tissue-specific gene expression during development. Key differences from prokaryotes include the lack of operons and more complex regulation in eukaryotes.
3) Gene expression can be regulated short-term through transcriptional control, as seen in yeast galactose-utilizing genes, or long-term for development through mechanisms like chromatin remodeling.
Drought tolerance in plants involves three main mechanisms: morphological, physiological, and genetic/molecular. Morphological mechanisms include drought escape and avoidance strategies like early reproduction or reduced water loss through waxy leaves. Physiological mechanisms regulate water use and loss, like stomatal closure and osmotic adjustment. Genetic and molecular mechanisms change gene expression, upregulating genes that produce proteins protecting cells from stress and regulating hormone signaling and transcription factors that control stress response pathways. Together these overlapping mechanisms help plants adapt and survive periods of low water availability.
RNAi – Mechanism and Its Application In Crop Improvementkundan Jadhao
This document summarizes an RNAi presentation on crop improvement using RNA interference. The 3-sentence summary is:
RNA interference (RNAi) is a process of post-transcriptional gene silencing mediated by small RNA molecules. The presentation described the RNAi pathway and various applications of RNAi technology in crop improvement, including increasing nutrient levels, developing virus and pest resistance, and reducing anti-nutritional compounds. Several case studies were provided that demonstrated how RNAi has been used to successfully modify traits in different crops like maize, cotton, coffee, and banana.
This document provides an overview of flower development. It begins with an introduction to the structure and anatomy of flowers. It then discusses the signals and environmental factors that trigger flowering, including photoperiodism. The document outlines the genetic control of flowering and describes several models of floral organ development, including the ABC hypothesis model. It provides details on the genes responsible for development in each floral whorl based on the ABC model and examples of mutations affecting these genes.
Brassinosteroids are a class of plant hormones that promote growth and development. Over 70 types of brassinosteroids have been isolated from various plant species, including angiosperms, gymnosperms, pteridophytes, bryophytes and algae. They are found in plant tissues and organs such as shoots, seeds, pollen and grains. Brassinosteroids have been shown to increase crop yields, enhance resistance to stresses, and improve plant growth. They are being commercialized for use in agriculture to boost productivity of important crops.
Cellular signal transduction pathways under abiotic stressSenthil Natesan
Abiotic stresses, especially cold, salinity and drought, are the primary causes of crop loss worldwide. Plant adaptation to environmental stresses is dependent upon the activation of cascades of molecular networks involved in stress perception, signal transduction, and the expression of specific stress-related genes and metabolites. Plants have stress-specific adaptive responses as well as responses which protect the plants from more than one environmental stress. There are multiple stress perception and signaling pathways, some of which are specific, but others may cross-talk at various steps (Knight & knight ,2001).Many cold induced pathways are activated to protect plants from deleterious effects of cold stress, but till date, most studied pathway is ICE-CBF-COR signaling pathway (Miura and Furumoto,2013 ) . The Salt-Overly-Sensitive (SOS) pathway, identified through isolation and study of the sos1, sos2, and sos3 mutants, is essential for maintaining favorable ion ratios in the cytoplasm and for tolerance of salt stress (shi .et al ,2002). Both ABA-dependent and -independent signaling pathways appear to be involved in osmotic stress tolerance (Nakashima and shinozaki, 2013) .ROS play a dual role in the response of plants to abiotic stresses functioning as toxic by-products of stress metabolism, as well as important signal transduction molecules and the ROS signaling networks can control growth, development, and stress response ( Mahajan,s and Tuteja, 2005) .
This document summarizes information about Arabidopsis thaliana, a small flowering plant that is widely used as a model organism. It describes A. thaliana's physical characteristics and life cycle, genome, and use in understanding flower development. Specifically, it outlines the ABC model of flower development in which three classes of genes (A, B, and C) interact to specify the four types of floral organs - sepals, petals, stamens, and carpels. Mutations in these genes result in homeotic transformations where one organ develops in place of another.
This document discusses Arabidopsis thaliana and its use in molecular biology research. Some key points:
- A. thaliana is well-suited for genetic research due to its small size, short life cycle, and large seed production. It was the first plant genome sequenced.
- Its genome of about 135 Mbp is among the smallest for higher plants. It contains 5 chromosomes useful for genetic mapping and sequencing.
- The document discusses forward and reverse genetics techniques used to study gene function in A. thaliana such as mutagenesis, screening mutants, positional cloning, and RNA interference. It provides examples of how these approaches have furthered understanding of plant genes and processes.
Major plant hormones include auxins, cytokinins, gibberellins, ethylene, and abscisic acid. Auxins promote cell elongation and root formation, inhibit lateral bud growth, and allow differential growth responses through areas of faster cell elongation. Cytokinins promote cell division and lateral bud growth. Gibberellins promote stem elongation and seed germination. Ethylene inhibits cell expansion and accelerates senescence and fruit ripening. Abscisic acid promotes stomatal closure and inhibits seed germination.
RNAi is a powerful, conserved biological process through which the small, double-stranded RNAs specifically silence the expression of homologous genes, largely through degradation of their cognate mRNA.
Programmed cell death in plants by shivanand b. koppadShivanand Koppad
This document discusses programmed cell death (PCD) in plants. It provides definitions of PCD and necrosis, and describes the differences between the two processes. PCD, also called apoptosis, is an actively controlled and genetically regulated process while necrosis is unregulated cell death in response to external stressors. The document outlines the history of studying PCD/apoptosis and discusses PCD pathways, regulators like caspases, and importance in plant development and response to the environment. It also provides a case study on PCD in tomato fruit in response to heat stress.
This document discusses plant biotechnology and how plants respond to biotic and abiotic stress. It defines homeostasis, stress, and the different types of stresses plants face including biotic (weeds, pathogens, insects) and abiotic (water, temperature, salt, air pollution). It describes how plants can respond to stress through resistance, avoidance, tolerance, or senescence/death. It provides examples of how plants respond to different abiotic stresses like drought, high temperatures, and salt. It also discusses the strategies pathogens use and the physical and induced defenses plants deploy against biotic stresses.
This document discusses regulation of gene expression in eukaryotes. It describes six main levels of control: transcription, RNA processing, mRNA transport, mRNA translation, mRNA degradation, and protein degradation. Key differences between prokaryotic and eukaryotic gene expression are explained, such as eukaryotes possessing nuclei and more complex regulation. Examples of short-term regulation including the GAL gene pathway in yeast and hormone response are provided.
The document discusses signal transduction, which is the process by which extracellular signals are converted into intracellular responses. There are six main steps: 1) synthesis and release of signaling molecules, 2) transport to target cell, 3) detection by receptor, 4) change in cell function triggered by receptor-signal complex, 5) removal of signal, and 6) termination of response. Signal transduction involves cell surface receptors and intracellular receptors that bind ligands and mediate specific cellular responses. Major types of signaling include endocrine, paracrine, and autocrine signaling.
Genetic engineering can be used to induce male sterility in plants by expressing genes that disrupt pollen development. Researchers have successfully transformed tobacco and oilseed rape plants with the barnase gene, which encodes an RNAse enzyme that destroys tapetal cells, preventing pollen formation. Restoration of fertility was achieved by co-expressing the barstar gene, which inhibits barnase. Similarly, expressing the argE gene in rice under a pollen-specific promoter induces male sterility when activated by an inducer, allowing hybrid seed production. Genetic engineering offers possibilities for more efficient hybrid seed systems in crops where traditional methods have not generated usable male sterility.
The document summarizes gene expression in eukaryotes. It discusses that gene expression involves transcription of DNA into mRNA which is then translated into proteins. It describes the key components of transcription including promoters, transcription factors, RNA polymerase, and the formation of the preinitiation complex. It also discusses mechanisms of regulating transcription, such as chromatin remodeling, transcription factors, enhancers, and transcriptional activators.
This summarizes an essay on the plant Arabidopsis thaliana and its response to environmental stresses like drought and high salt levels. When stressed, the plant hormone ABA is produced, which triggers adaptive responses to help the plant survive. ABA regulates many transcriptional factors, including members of the bZIP family that are expressed during stress. The essay discusses the domains of AREB/ABF transcription factors and how they are phosphorylated and regulated in response to ABA and stresses.
Quantitative PCR (qPCR), also known as real-time PCR, is a laboratory technique used to quantify the amount of a specific DNA sequence in a sample. Some key points about how qPCR is used to determine the amount of DNA:
- qPCR works by amplifying a target DNA sequence over multiple cycles. It monitors the amplification in real-time using fluorescent dyes or probes.
- The fluorescent signal increases as more DNA is amplified. The point at which the fluorescence crosses a defined threshold is called the cycle threshold (Ct).
- Samples with more DNA copies of the target sequence will reach the threshold earlier in the amplification process (lower Ct value). Samples with fewer copies will reach it later (higher Ct value).
The document summarizes genetic and mutational characterization of the relV gene of Vibrio cholerae, which encodes a small alarmone synthetase protein called RelV. Key findings include:
1) Site-directed mutagenesis identified five amino acid residues (K107, D129, R132, L150, E188) in the RelA-SpoT domain of RelV that are essential for its (p)ppGpp synthetase activity.
2) Progressive deletion analysis determined the functional N-terminal boundary of RelV to be amino acid 59 and the C-terminal boundary to be amino acid 248, indicating that flanking sequences of the RelA-SpoT
1. The document describes using TILLING by sequencing in mung bean to identify mutations in genes related to plant architecture, such as flowering time and branching, in order to develop a plant type suited for mechanical harvesting with increased productivity.
2. Key candidate genes studied include GIGANTEA, RAMOSUS, CONSTANS, LEAFY, and TERMINAL FLOWERING 1b, which regulate flowering time and branching.
3. The TILLING by sequencing workflow included EMS mutagenesis, generation of a TILLING population, sequencing of candidate gene fragments, and identification of mutations, some of which were predicted to be damaging by bioinformatics analysis.
“Transcription factor as signaling regulatory tools for improving growth proc...AKSHAYMAGAR17
The document discusses several transcription factors and their roles in plant growth processes. It provides case studies on:
1) Tb1 which regulates lateral branch development in maize by repressing axillary growth.
2) Shi4 and SHAT1 which regulate grain shattering in rice by specifying abscission zone development.
3) GA1 which regulates dwarfing as a gibberellin insensitive dwarf gene in apple rootstocks.
4) MADS-box and KNOX genes which regulate flowering development through involvement in stress responses and developmental plasticity.
5) HAT4 which regulates shade development as a member of the HD-ZIPII family involved in shade-induced growth responses.
This study analyzed genetic variation in the DREB1A and DREB1B genes across 20 rice genotypes with different responses to low temperature stress. Sequencing found a few single nucleotide polymorphisms and indels in the coding and non-coding regions of the genes. However, none of the variations were found to be associated with cold tolerance based on seedling stage phenotypes. Expression analysis also found induction of both genes in tolerant and susceptible genotypes upon cold treatment. Comparison of 400 rice varieties additionally found low nucleotide diversity in DREB1A, DREB1B and a related gene MYB2 compared to other rice genes, suggesting the cold response pathway is highly conserved. Overall, the results indicate natural allelic variations in DREB
2007 overexpression of an r1 r2r3 myb gene, osmyb3r-2,Agrin Life
Overexpression of the rice gene OsMYB3R-2, which encodes an R1R2R3 MYB transcription factor, increases tolerance to freezing, drought, and salt stress in transgenic Arabidopsis plants. OsMYB3R-2 expression is induced by cold, drought, and salt stress. Transgenic Arabidopsis plants overexpressing OsMYB3R-2 showed increased tolerance to these stresses compared to wild-type plants. The overexpression also led to higher expression of several cold-related genes. This suggests that OsMYB3R-2 acts as a master switch that increases stress tolerance.
1. The document describes a study that used Targeting Induced Local Lesions in Genomes (TILLING) by sequencing to identify mutations in candidate genes involved in flowering time and plant architecture in mung bean.
2. The researchers generated a TILLING population by treating mung bean seeds with the mutagen EMS. They then identified 10 mutations across 5 candidate genes (GIGANTEA, RAMOSUS, CONSTANS, LEAFY, and TERMINAL FLOWERING 1b) using Illumina sequencing and bioinformatics.
3. The mutations were analyzed in silico and some were predicted to have damaging effects on protein function. Selected mutants were then validated using Sanger sequencing.
Genome–Wide Analysis and Expression Pattern of the AP2/ERF Gene Family in Kiw...Agriculture Journal IJOEAR
—APETALA2/ethylene response factor (AP2/ERF) transcription factors play important roles in the response to abiotic stresses. It is now possible to identify all of the AP2/ERF genes in the kiwifruit genome because the kiwifruit genome project has been completed. 183 AP2/ERF genes were identified and compared with AP2/ERF genes from Arabidopsis in this study. The 183 AP2/ERF kiwifruit genes were classified into four subfamilies: DREB (64), ERF (94), AP2 (19) and RAV (5), as well as one soloist. RNA-sequence and Quantitative RT-PCR (qRT-PCR) analysis results showed that 20 genes were responsive to waterlogging stress, suggesting that AP2/ERF transcription factors play important roles in the response to waterlogging stress in kiwifruit
The document summarizes research on the regulation of gene expression in Mycobacterium tuberculosis through sigma factors and their anti-sigma factor partners. It discusses how σF, a stress response sigma factor in M. tuberculosis, is regulated post-translationally by its anti-sigma factor UsfX. Experiments show that UsfX binds nucleotides like ATP and GTP and has ATPase and GTPase activity. UsfX was found to interact with σF in a 2:1 stoichiometric ratio, forming a complex.
The document summarizes a study that analyzed the expression of the At1g17950 gene in diploid and tetraploid Arabidopsis thaliana plants under salt stress conditions. The study found that the At1g17950 gene, which encodes a MYB transcription factor involved in abscisic acid production and response to salt stress, was expressed more highly in diploid plants than in tetraploid plants. This suggests that tetraploid A. thaliana have greater resistance to salt stress due to lower expression of the At1g17950 gene, while diploid plants are more sensitive to salt stress due to higher expression of this gene.
This document summarizes a study comparing the transcriptomes of drought tolerant and susceptible rice cultivars. 436 genes were differentially expressed between the cultivars. The tolerant cultivar had genes enriched in stress response pathways while the susceptible cultivar had genes involved in cell death. Photosynthesis genes showed evidence of strong positive selection, suggesting their importance in drought adaptation. Validation experiments confirmed gene expression differences and most sequence variations called between the cultivars. The study highlights the role of photosynthesis in rice domestication for drought tolerance.
2010 expression of a truncated form of yeast ribosomal protein l3Agrin Life
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Transcription factors as key regulators of gene expression
1. Transcription factors as key regulators
of gene expression
M.Sc. Master Seminar
Anjani Kumar
(BAC/M/PBG/003/15-16)
Department of Plant Breeding and Genetics,
Bihar Agricultural College,
BAU, Sabour
2. What is a Transcription Factor (TF)?
Any protein other than RNA Polymerase that
is required for transcription
These are of two types:
General transcription factors Required for the binding of the
RNA pol to the core promoter and its progression to the
elongation stage Are necessary for basal transcription
Regulatory transcription factors Serve to regulate the rate of
transcription of nearby genes They influence the ability of
RNA pol to begin transcription of a particular gene. These
factors recognize cis-regulatory elements located near the
core promoter.
3. The RNA-transcribing enzyme, RNA polymerase II (red), requires general transcription factors (TFII) D, A, B, F, E, and H
(blue), which themselves consist of multiple subunits, to recognize the transcription start site via the TATA box or related
sequences in the core promoter. The sum of these factors, known as the pre-initiation complex (PIC), is required for basal
transcription.
Transcription factors (green) bind to specific DNA sequences (red) via their DNA-binding domain (DBD) and modulate the
rate of transcription via their transactivation domain(s) (TAD).
[Source: http://cro.sagepub.com/content/15/5/282/F1.expansion.html]
A simplistic view of regulatory mechanisms of gene
transcription
DNA binding domain – DBD
Binds specific sequence of
base pairs
Transcriptional activation
domain – TAD
Interacts with basal TF
directly with RNA pol II
Protein-protein interaction
domain – PPID
Interaction with other
transcription factors
4. Source: PlantTFDB @ CBI, PKU ,10/25/2016
Major TF family and genes reported in plants….
5. Source: http://voices.nationalgeographic.com/2009/03/23/corn_domesticated_8700_years_ago/
Teosinte branched1
(Tb1) transcription
factor in
domestication of
maize
When Tb1 is expressed, it represses the outgrowth of lateral branches; maize plants carrying loss of function
alleles produce numerous lateral branches (tillers). During the domestication of teosinte to produce maize,
an allele was selected that altered the regulation of Tb1, increasing its expression in primary auxiliary
meristems (Doebley et al., 1997)
Why these TFs are so important....???
6. The semidwarf varieties of wheat are short
due to a mutation in at least one of two
Reduced height-1 loci (Rht-B1 and Rht-D1]
[Karen Century et al., 2008]
Peng et al. (1999) elegantly demonstrated
that Rht-B1 and Rht-D1 were orthologs of
the Arabidopsis GIBBERELLIN INSENSITIVE
(GAI) gene, a member of the GRAS family
of TFs, which function as transcriptional
repressors of growth.
Major yield gains achieved by conventional plant breeders,
ALSO had role of TFs
Source: http://www.newhallmill.org.uk/wht-rht.htm
Fig. The picture shows the wheat variety "Mercia" with a control and
four lines with different Rht genes.
Rht-0, with no reduced-height gene, is used as the control for height
comparison.
Rht-1 and Rht-2 typically produce semi-dwarf plants, two-thirds the
height of the control.
Rht-3 and Rht-12 typically produce dwarf plants, one-third the height of
the control.
7. • Two TFs have been identified as playing a major role in reducing grain
shattering in domesticated rice plants.
• One of these was isolated as a quantitative trait locus (QTL) in a cross
between a shattering-type ‘Indica’ cultivar and a non-shattering type
‘Japonica’ (Konishi et al., 2006).
• This gene, qSH1, encodes a BEL1-type homeodomain protein that is
orthologous to Arabidopsis REPLUMLESS (RPL), which is involved in the
formation of an abscission zone in the Arabidopsis silique.
• The other TF affecting this trait is shattering4 (sh4), allelic to sha1 (Li et al.,
2006; Lin et al., 2007). SH4 is a member of the trihelix family of plant-specific
TFs and was isolated as a major QTL for shattering in a cross between O.
sativa and Oryza rufipogon.
Role of TF in Rice shattering
8. Review of literature suggests that TFs plays different regulatory role in
plants (stress tolerance, defense, metabolite biosynthesis etc.
T.F spp. Function Reference
AtMYB096 Arabidopsis thaliana Drought tolerance (ABA and
JA–mediated)
Seo et al. 2009
OsMYB55 Oryza sativa Heat stress tolerance El-kereamy et al.
2012
ScMYBAS1 Saccharum officinarum Drought and salt tolerance Prabu and Theertha
2011, Prabu and
Prasad 2012
AtMYB011/AtMYB
012/ AtMYB111
Arabidopsis thaliana Phenylpropanoid pathway/
Flavonol biosynthesis
Stracke et al. 2007
AtMYB44 Arabidopsis thaliana Plant defense response
against aphid
Liu et al. 2010
AtMYB15 Arabidopsis thaliana cold stress tolerance Agarwal et al. 2006
GmMYB Glycine max salt, drought and/or cold
stress
Liao et al. 2008
9. T.F spp. Function Reference
WRKY57
OsWRKY11
OsWRKY13
A . thaliana
Oryza sativa
Oryza sativa
drought tolerance
drought and heat tolerance
bacterial blight Xanthomonas oryzae pv
oryzae and the fungal blast
Magnaportha grisea
Lindemose,Søren
et al.2013
Qiu et al., 2007,
2008
NTL6, PR1, PR2, PR5
Arabidopsis thaliana
Positiveregulatorofpathogenresistance
against P. syringae
Seo et al., 2010
DREB Oryza sativa drought & salt Dubouzet et al.
2003
CBF Brassica napus, Triticum
aestivum Lycopersicon
Esculentum
tolerance to freezing and
drought/low-temperature exposure
Jaglo et al., 2001).
WD40 Zea mays (maize) Regulation of anthocyanin pathway in
seed aleurone and scutellum
Carey et al. (2004)
WD40 Vitis vinifera (grape) Contributes to the accumulation of
anthocyanins
Matus et al. (2010)
OsNAC6, Oryza sativa Slightly increased tolerance to rice blast
disease
Nakashima et al.,
2007
bZIP A . thaliana Drought,salt,cold Choi et al.2000
AP2/EREBP Arabidopsis and rice drought tolerance Karaba et al., 2007
Continue….
10. How these TFs works in plants….
(Front. Plant Sci., 09 February 2016 | http://dx.doi.org/10.3389/fpls.2016.00067)
11. (Int. J. Mol. Sci. 2013, 14(4), 7515-7541; doi:10.3390/ijms14047515)
12. Regulation of Flavonoid biosynthesis by TFs
(Shutian Li et al. 2014)
Figure . The biosynthetic pathway for flavonols, anthocyanins, and PAs in Arabidopsis. This pathway starts with the general phenylpropanoid metabolism
and subsequent steps are catalyzed by a series of structural enzymes leading to the biosynthesis of 3 final end products, including flavonols,
anthocyanins, and PAs. The early biosynthetic genes (EBGs) are activated by 3 functionally redundant R2R3-MYB proteins (MYB11, MYB12, and
MYB111), whereas the expression of the late biosynthetic genes (LBGs) requires the transcriptional activation activity of the R2R3-MYB/bHLH/WD40
(MBW) complex. Enzymes are denoted in uppercase and corresponding genetic loci are indicated in italic lowercase letters. PAL, phenylalanine
ammonia-lyase; C4H, cinnamate 4-hydroxylase; 4CL, 4-coumarate:CoA ligase; CHS, chalcone synthase; CHI, chalcone isomerase; F3H, flavanone 3-
hydroxylase; F3′H, flavanone 3′-hydroxylase; DFR, dihydroflavonol 4-reductase; LDOX, leucoanthocyanidin dioxygenase; ANR, anthocyanidin reductase;
tt, transparent testa; ban, banyuls.
TFs
TFs
13. Fig. Phenotype of strawberry plants transformed with 35S:FvMYB10,FvMYB10 RNAi, and wild-type controls. Growth
and pigmentation of strawberry plants transformed with 35S:FvMYB10, FvMYB10 RNAi, and wild-type controls (A). Detailed phenotype of
strawberry leaves,flowers and fruit of 35S:FvMYB10,FvMYB10 RNAi, and wild-typecontrols.Linesof35S:FvMYB10 had pigmented leaves,
petioles, stigmas and petals, and mature fruit had darkred/purple skin and red flesh. The mature fruit of FvMYB10 RNAi lines had white
skin and white flesh, and the only pigmented tissue was the petioles (B).
(Kui Lin-Wang et al.2014 )
MYB
FOR ENHANCED
PIGMENTATION
Application
of TFs
14. Fig. Freezing tolerance of cbf2 mutant plants. Three-week-old WT and cbf2 plants grown under long-day
photoperiods at 20°C were exposed to different freezing temperatures for 6 h. Freezing tolerance was
estimated as the percentage of plants surviving each specific temperature after 7 days of recovery under
unstressed conditions. (A) Tolerance of nonacclimated plants. (B) Representative nonacclimated WT
and cbf2 plants 7 days after being exposed to -6°C for 6 h. (C) Tolerance of cold-acclimated (7 days at
4°C) plants. (D) Representative cold-acclimated WT and cbf2 plants 7 days after being exposed to -10°C
for 6 h. In A and C, data are expressed as means of three independent experiments with 50 plants each.
Bars indicate SE Fernando Novillo et al.2004,
CBF for freezing
tolerance
15. Figure 4. Stress-tolerance assays of AaDREB1 overexpressing transgenic Arabidopsis. Ten-day-old
seedlings of AaDREB1 transgenic lines (T17, T122, T196) and the empty vector control plants (WT) were
treated with either 100 mM NaCl for 12 days, 10% PEG for 10 days, or exposed to 4 °C for 20 h, then
grown under normal growth conditions for 3 days. Scale bar represents 1.5 cm
(Zong et al.2016)
DREB for
salt,
drought and
cold
16. Overexpression of AtDREB1 Improved Salt Tolerance of Transgenic Rice
Plants
Fig. Salt tolerance analyses of wild type plants and‑ ‑ AtDREB1 transgenic rice. (A) The phenotype of
two week old‑ ‑ AtDREB1 transgenic lines and wild type rice plants grown in the glasshouse under normal‑
growth conditions before being transferred to 150 mM NaCl for 16 days.
Int. J. Mol. Sci. 2016, 17(4), 611; doi:10.3390/ijms17040611
(hp://dx.doi.org/10.3390/ijms17040611
Jun Mei Zong et al.2016‑
17. Improved drought resistance of SNAC1-overexpressing transgenic rice at reproductive stage. (a)
Overexpression contruct (Upper) and RNA gel blot analysis of SNAC1 in transgenic plants and
the WT (Lower). (b) Southern blot analysis of transgenic plants using hygromycin resistance
gene as a probe. (c) Appearance of one positive (S19) and one negative (S18) transgenic
families in the field with severe drought stress. (d) Cosegregation of SNAC1-overexpressing
(RNA gel blot analysis) with the improved drought tolerance in the T1 family of S19. SS(%), seed-
setting rate
Hu et al. PNAS 2006;103:12987-12992
Improved drought resistance of SNAC1-overexpressing transgenic rice at reproductive stage.
NAC for drought
resistance
18. Prospect of Application of TF in Crop Improvement
1. IN CROP DOMESTICATION AND BREEDING
Because of their nature as master switches for major regulatory networks and their prior
role in the domestication of many crop species, TFs are predicted to be among the
best and safest candidate loci for engineering these traits.
2. ENHANCING NUTRIENT USE EFFICIENCY
An example of the successful engineering of enhanced nitrogen uptake using a TF was
reported by Yanagisawa et al. (2004), the overexpression of maize Dof1 gene, which was
known to be involved in organic acid metabolism, to create transgenic Arabidopsis plants
that showed increases in free amino acid content and total nitrogen uptake, as well as
improved growth under low nitrogen conditions.
3. IMPROVEMENT IN YIELD POTENTIAL
There are a number of approaches that might be taken to boost intrinsic yield,
including increasing photosynthetic capacity, modifying plant architecture,
controlling disease and pest, and enhancing the plant’s rate of growth
19. •Though, TFs are a useful candidate for qualitative and quantitative
trait improvement in plants.
•TF technologies often require optimization, either to reduce
unwanted side effects such as growth retardation or to enhance the
desired trait to the level at which it is of commercial value.
•Optimization require a tissue-specific, developmental, or inducible
promoters rather than the usual constitutive promoters, to limit
expression of the transgene to the appropriate tissues or
environmental condition
•Lengthy and costly process of developing a new commercial
genetically MODIFIED CROP
•Securing approvals from regulatory authorities
Challenges while using TFs in agricultural product
development
20. Conclusion
• This is well proven that TFs acts as master switches for
major regulatory networks.
• They regulate the co-ordinated expression of several genes
in a multi-genic pathways, thereby a single TF may be
sufficient for engineering the target traits.
• Taken together, various roles played by TFs in plants, these
could be potential source of candidate genes for modifying
complex traits in crops.
• TF-based strategies appear to be very promising and could
play a major role in development of genetically engineered
crops.
21. References
Konishi S, Izawa T, Lin SY, Ebana K, Fukuta Y, Sasaki T, Yano M (2006). An SNP caused loss of see
shattering during rice domestication. Science 312: 1392–1396
Doebley J, Stec A, Hubbard L (1997) The evolution of apical dominance in
maize. Nature 386: 485–488
Peng J, Richards DE, Hartley NM, Murphy GP, Devos KM, Flintham JE, Beales J, Fish LJ,
Worland AJ, Pelica F, et al (1999) ‘Green revolution’ genes encode mutant gibberellin response
modulators. Nature 400: 256–261
Lin Z, Griffith ME, Li X, Zhu Z, Tan L, Fu Y, Zhang W, Wang X, Xie D, Sun C (2007) Origin of
seed shattering in rice (Oryza sativa L.). Planta 226: 11–20
Li C, Zhou A, Sang T (2006) Rice domestication by reducing shattering. Science 311: 1936–
1939
Shutian Li (2013) Transcriptional control of flavonoid biosynthesis. 10.4161/psb.27522
KuiLin-Wang1, TonyK.McGhie2, MindyWang1, YuhuiLiu3, BenjaminWarren1, RoyStorey4,
RichardV.Espley1 and AndrewC.Allan1,5 (2014) Engineering the anthocyanin regulatory
complex of strawberry (Fragaria vesca) Plant science doi: 10.3389/fpls.2014.00651
22. KuiLin-Wang1, TonyK.McGhie2, MindyWang1, YuhuiLiu3, BenjaminWarren1, RoyStorey4,
RichardV.Espley1 and AndrewC.Allan1,5 (2014) Engineering the anthocyanin regulatory
complex of strawberry (Fragaria vesca) Plant science doi: 10.3389/fpls.2014.00651
Fernando Novillo, Jose´ M. Alonso, Joseph R. Ecker, and Julio Salinas (2003) CBF2DREB1C is
a negative regulator of CBF1DREB1B and CBF3DREB1A expression and plays a central role in
stress tolerance in Arabidopsis. Pnas doi10.1073pnas.0303029101
Honghong Hu, Mingqiu Dai, Jialing Yao, Benze Xiao, Xianghua Li , Qifa Zhang, and Lizhong
Xiong (2006) Overexpressing a NAM, ATAF, and CUC (NAC) transcription factor enhances
drought resistance and salt tolerance in rice. pnas.0604882103
Jun Mei Zong , Xiao Wei Li , Yuan Hang Zhou, Fa Wei Wang, Nan Wang, Yuan Yuan Dong,‑ ‑ ‑ ‑ ‑
Yan Xi Yuan, Huan Chen, Xiu Ming Liu, Na Yao and Hai Yan Li (2016) The‑ ‑ ‑ AaDREB1
Transcription Factor from the Cold Tolerant Plant‑ Adonis amurensis Enhances Abiotic Stress
Tolerance in Transgenic Plant. Int. J. Mol. Sci. 17(4), 611; doi:10.3390/ijms17040611
Yanagisawa S, Akiyama A, Kisaka H, Uchimiya H, Miwa T (2004) Metabolic engineering with
Dof1 transcription factor in plants: improved nitrogen assimilation and growth under low
nitrogen conditions. Proc Natl Acad Sci USA 101: 7833–7838