1. Dendritic cells are antigen presenting cells that express pattern recognition receptors including toll-like receptors. 2. Dendritic cells play key roles in immunity through TLR-mediated antigen recognition, activation and migration, and antigen presentation to activate adaptive immunity. 3. TLR signaling induces cytokine secretion and phosphorylation cascades that upregulate co-stimulatory molecules and MHC II, allowing dendritic cells to present antigens to naive T cells and induce both CD4 and CD8 T cell activation and differentiation.

1. In Course Presentation
Yogyakarta, May 2th, 2012
Tenri A.W.
Musketeers Course 2012

2. MAJOR CONCEPT

4. “The BIG IDEA”

5. MAJOR CONCEPT

7. WHAT IS DENDRITIC CELL?
DENDRITIC CELL (DC)
IS AN ANTIGEN PRESENTING CELL (APC)
DC IS EXPRESSING SEVERAL PATTERN-RECOGNITION RECEPTOR
(PRR), INCLUDING TOLL-LIKE RECEPTOR (TLR)

8. MAJOR CONCEPT

10. ROLE DENDRITIC CELL IN IMMUNITY
DC IN TLR-MEDIATED
ANTIGEN
RECOGNITION
DC IN ACTIVATION
AND MIGRATION
DC IN ANTIGEN
PRESENTATION AND
ADAPTIVE
IMMUNITY
ACTIVATION

11. ROLE DENDRITIC CELL IN IMMUNITY
DC IN TLR-MEDIATED
ANTIGEN
RECOGNITION
DC IN ACTIVATION
AND MIGRATION
DC IN ANTIGEN
PRESENTATION AND
ADAPTIVE
IMMUNITY
ACTIVATION

12. TOLL-LIKE RECEPTOR

13. TLR, LIGAND, AND ADJUVANT
(DUIN ET AL., 2006)

14. ROLE DENDRITIC CELL IN IMMUNITY
DC IN TLR-MEDIATED
ANTIGEN
RECOGNITION
DC IN ACTIVATION
AND MIGRATION
DC IN ANTIGEN
PRESENTATION AND
ADAPTIVE
IMMUNITY
ACTIVATION

15. TLR SIGNALING PATHWAY
SIGNALING
MOLECULE
TLR
CYTOKINE
SECRETION SIGNALING PHOSPORILATION
CASCADE
PATHWAY
TRANSCRIPTION
FACTOR
ACTIVATION

16. TLR SIGNALING PATHWAY (CONT)

18. TLR SIGNALING INDUCE
CCR7 EXPRESSION

19. TLR SIGNALING UPREGULATE MHC II
AND CO-STIMULATORY MOLECULE

21. ROLE DENDRITIC CELL IN IMMUNITY
DC IN TLR-MEDIATED
ANTIGEN
RECOGNITION
DC IN ACTIVATION
AND MIGRATION
DC IN ANTIGEN
PRESENTATION AND
ADAPTIVE
IMMUNITY
ACTIVATION

22. DC PRESENT ANTIGEN
TO NAÏVE T CELL

23. DC INDUCE BOTH CD 4 & CD 8
T CELL ACTIVATION

24. DC INDUCE CD 4 T-CELL
DIFFERENTIATION
IN MOST CASE, VIA TLR
ACTIVATION, INDUCE
DEVELOPING T HELPER 1
RESPONSE
(TRINCHIERY, 2003)

25. TH 1 CELL in INTRACELLULAR DEFENSE
TH 1 INDUCE Ig G, NK
CELL, MACROPHAGE AND OTHER CELL
ACTIVATION IN INTRACELLULAR
DEFENSE MECHANISM

26. MAJOR CONCEPT

28. VACCINE IN INFECTIOUS DISEASE

29. MYCOBACTERIUM BCG VACCINE
• BCG VACCINE IS USING TLR 2 AND TLR 4 – MYD88 PATHWAY

30. TLR 2 - TLR 4 – MYD88 PATHWAY

31. VACCINE IN MELANOMA CANCER
• MELANOMA CANCER VACCINE IS DNA VACCINE, USING TLR 9 – MYD88 PATHWAY

32. PRINCIPLE OF TLR SIGNALING

33. THANK YOU TO THESE GUYS

34. MAJOR CONCEPT

36. SUMMARY