International Conference on Biotechnology | 13 - 14 November 2012 | Bogor, Indonesia.

2. Background
Materials & Methods
Result & Discussion
Concluding Remarks
Recommendation

8. Background
Materials & Methods
Result & Discussion
Concluding Remarks
Recommendation

12. APPRAISE (A) SYNTHESISE (S) TRANSFERABLE (T)FIND (F)

13. APPRAISE (A) SYNTHESISE (S) TRANSFERABLE (T)FIND (F)
Diagnostic Detection Dengue
Infection
UrineO
R
A
N
D
A
N
D
PubMed=18 ProQuest=11 Ovid=16 Cochrane=17
Total=62
Total=21
Eliminate
same article
Total=4
Abstract screening
Inclusion Criteria:
 dengue infection
 urine sample
 diagnostic method
Abstract screening
Exclusion Criteria:
 immunoassay
 animal model
 sample < 30
Total=1
Fulltext Article
Hirayama et al. (2012)
Critical Appraisal
 relevance
 validity

14. Background
Materials & Methods
Result & Discussion
Concluding Remarks
Recommendation

15. APPRAISE (A) SYNTHESISE (S) TRANSFERABLE (T)FIND (F)

16. APPRAISE (A) SYNTHESISE (S) TRANSFERABLE (T)FIND (F)

17. APPRAISE (A) SYNTHESISE (S) TRANSFERABLE (T)FIND (F)

18. APPRAISE (A) SYNTHESISE (S) TRANSFERABLE (T)FIND (F)

19. Background
Materials & Methods
Result & Discussion
Concluding Remarks
Recommendation

20. APPRAISE (A) SYNTHESISE (S) TRANSFERABLE (T)FIND (F)

21. Background
Materials & Methods
Result & Discussion
Concluding Remarks
Recommendation

23. • Simple
• Rapid (high efficiency)
• Specific (high specificity)
• Cost effective

24. Molecular
Method
Done in isothermal
(65-67o)
Using 6-8 Primers
(Highly Sensitive & Specific)
Notomi et al. (2000)
Notomi et al. (2000)

25. Molecular
Method
Done in isothermal
(65-67o)
Using 6-8 Primers
(Highly Sensitive & Specific)
Notomi et al. (2000)
Notomi et al. (2000)

26. Molecular
Method
Done in isothermal
(65-67o)
Using 6-8 Primers
(Highly Sensitive & Specific)
Notomi et al. (2000)
Notomi et al. (2000)

31. Simple
Procedure
Using waterbath
Rapid test
(less than 1 hour)