This document discusses the need for establishing varnasrama dharma in society according to scriptural teachings. It notes that varnasrama dharma properly balances the material and spiritual aspects of life by dividing society according to natural qualities and stages of life. Establishing varnasrama would help create an ideal society where people can more easily advance spiritually by performing their prescribed duties. While the basic divisions of varnasrama always exist, in the current age they are not properly understood or practiced. Reintroducing varnasrama according to scriptural norms would help harmonize society and facilitate spiritual progress.
Author : HH RP Bhakti Raghava Swami
E-Mail : Bhakti.Raghava.Swami@pamho.net
Date Produced : 2015
Editor: Sriman Prafullakumar Seelam
Serial No: 1 of 54
Author : HH RP Bhakti Raghava Swami
E-Mail : Bhakti.Raghava.Swami@pamho.net
Date Produced : 2015
Editor: Sriman Prafullakumar Seelam
Serial No: 1 of 54
BHAGVAD GITA CHAPTER 6 FLOWCHARTS
Chapter six of the Gita,
Abhyasa Yoga,
alternately entitled in Sanskrit
“Dhyan Yoga” (“The Yoga of Meditation”)
UNITIVE CONTEMPLATION,
or “ Atma Sanyam Yoga”
(“The Yoga of Complete Concentration on the Self”),
the "yoga of controlling the Âmâ
Learn the basics of Bhakti Yoga and begin your practice with confidence and understanding. Learn about Krsna, Radha, mantra meditation and more from brief presentation on Bhakti Yoga.
Bhagvad gita chapter 1 , Arjuna-Vishada yoga (The yoga of the dejection of Ar...Medicherla Kumar
Revised and updated in 2021. The flowcharts and overview black and white for printing.
• Chapter 1: Arjuna-Vishada yoga (The yoga of the dejection of Arjuna) - 47 verses. Arjuna's depression is presented as a system of yoga. When Arjuna sees his near and dear ones on the opposing army side of the Kurus, he loses morale and decides not to fight (1:20-27). Arjuna presents Krishna his arguments for refusing to fight (1:28-47). Basically, he fears the sinful reactions of killing.
Revised-2021
Dr.Medicherla Shyam Sunder Kumar.
samc108@gmail.com
• Chapter 16: Daivasura-Sampad-Vibhaga Yoga (The yoga of discrimination between the divine and the demonical properties) - 24 verses.
Krishna indicates the divine and the demonic traits present in human nature. After mentioning twenty-six godly qualities, Krishna explains the demoniac nature which degrades the soul through arrogant, ignorant, and conceited pursuits of sense gratification and power (16:1-18).
He counsels that, in order to attain the supreme destination, one must give up lust, anger and greed (16:21-22).
Assisted by buddhi (intellect) and supported by scriptural confirmation, one should discern right and wrong and act appropriately (16:24).
Srimad Bhagavatam
Srimad Bhagavata Purana_The ripened fruit of all Vedas
In the great Naimasharaya forest, in the assembly of the exalted rishis headed by Rishi Śaunaka, the perfect one – Śrī Sūta Gosvāmi recited to them – the Bhāgavat Purāna as sung by Śrī Sukadeva Goswami to Maharaj Parikshit on the banks of Ma Ganga near Hastinapur, amidst the greatest confluence of enlightened sages from all over the universe. Śrī Sukadeva, a self-realized perfect soul – an Avadhuta, narrated the greatest of purana when only 16 years old, but more learned than all the realized rishis present. Śrī Sukadeva studied the Bhāgavatam through upanishad from his father, compiler of Śrīmad-Bhāgavatam – Bhagvān Veda Vyāsadeva (Bādarayana), Lord’s avataar.
Sukadev Goswami narrates Bhagavatam at the end of Dwaparayuga
idaṁ bhāgavataṁ nāma
purāṇaṁ brahma-sammitam
adhītavān dvāparādau
pitur dvaipāyanād aham
“At the end of the Dvāpara-yuga, I studied this great supplement of Vedic literature named Śrīmad-Bhāgavatam, which is equal to all the Vedas, from my father, Śrīla Dvaipāyana Vyāsadeva.”
~ Śrī Sukadeva Goswami, Śrīmad-Bhāgavatam (2.1.8)
Suta Goswami narrates Bhagavatam at Naimasharanya headed by Sunaka Rishi
idaṁ bhāgavataṁ nāma
purāṇaṁ brahma-sammitam
uttama-śloka-caritaṁ
cakāra bhagavān ṛṣiḥ
niḥśreyasāya lokasya
dhanyaṁ svasty-ayanaṁ mahat
This (idaṁ) scripture (purāṇaṁ) named (nāma) Śrīmad-Bhāgavatam (bhāgavataṁ ) is the literary incarnation (brahma-sammitam) of God (uttama-śloka-caritaṁ), and it is compiled (cakāra) by Śrīla Vyāsadeva (ṛṣiḥ), the incarnation of God (bhagavān). It is meant for the ultimate good (niḥśreyasāya) of all people (lokasya), and it is all-successful (dhanyaṁ), all-blissful (svasty-ayanaṁ) and all-perfect (mahat).
~ Sūta Gosvāmi in Śrīmad-Bhāgavatam (1.3.40)
As God is all light, all bliss and all perfection, so also is Śrīmad-Bhāgavatam. One can derive from its study all benefits that are possible to be derived from the personal presence of the Lord. It carries with it all the transcendental blessings of Lord Śrī Kṛṣṇa that we can expect from His personal contact. Śrīmad-Bhāgavatam should be worshiped as respectfully as we worship the Lord.
Spotless, Unlimited, Lord Krsna’s Representative!
Lord Śrī Caitanya Mahāprabhu has declared that Śrīmad-Bhāgavatam is the spotless (amalam) representation of all Vedic knowledge and history. What can be said more about this maha-purana? It is directly the Lord Himself – Krishna, Uttama-śloka in His absolute literary incarnation.
Mahaprabhu while giving confidential instructions to Sanatana Goswami says – “Just try to understand the real nature of Srimad-Bhagavatam. It is the sound representation of the Supreme Lord Krishna; therefore Srimad-Bhagavatam is not different from Krishna. Krishna is unlimited,
Bhagvad gita chapter 17 , (Revised 2021) The flowcharts and overview Medicherla Kumar
Revised and updated in 2021.
Bhagvad gita chapter 17 , The flowcharts and overview black and white for printing.
Chapter 17: Sraddhatraya-Vibhaga Yoga (The yoga of the classification of the threefold faith) - 28 verses. Krishna mentions three divisions of beliefs, thoughts, deeds and even eating habits corresponding to the three gunas (17:4-10).
• ,
• Faith in Gita ,
Shraddha or Faith
The flowcharts and overview
BHAKTI YOGA By
Dr. Pankaj Shukla
(AYURVEDACHARYA)
ॐ सह नाववतु।
सह नौ भुनक्तु।
सह वीर्यंकरवावहै।
तेजस्वि नावधीतमस्तुमा विद्विषावहै।
ॐ शान्तिः शान्तिः शान्तिः
What is Bhakti ?
Types of Bhakti
How to Cultivate Bhakti
Bhavas in Bhakti
Nava-Vidha-Bhakti
Fruits of Bhakti
BHAGVAD GITA CHAPTER 6 FLOWCHARTS
Chapter six of the Gita,
Abhyasa Yoga,
alternately entitled in Sanskrit
“Dhyan Yoga” (“The Yoga of Meditation”)
UNITIVE CONTEMPLATION,
or “ Atma Sanyam Yoga”
(“The Yoga of Complete Concentration on the Self”),
the "yoga of controlling the Âmâ
Learn the basics of Bhakti Yoga and begin your practice with confidence and understanding. Learn about Krsna, Radha, mantra meditation and more from brief presentation on Bhakti Yoga.
Bhagvad gita chapter 1 , Arjuna-Vishada yoga (The yoga of the dejection of Ar...Medicherla Kumar
Revised and updated in 2021. The flowcharts and overview black and white for printing.
• Chapter 1: Arjuna-Vishada yoga (The yoga of the dejection of Arjuna) - 47 verses. Arjuna's depression is presented as a system of yoga. When Arjuna sees his near and dear ones on the opposing army side of the Kurus, he loses morale and decides not to fight (1:20-27). Arjuna presents Krishna his arguments for refusing to fight (1:28-47). Basically, he fears the sinful reactions of killing.
Revised-2021
Dr.Medicherla Shyam Sunder Kumar.
samc108@gmail.com
• Chapter 16: Daivasura-Sampad-Vibhaga Yoga (The yoga of discrimination between the divine and the demonical properties) - 24 verses.
Krishna indicates the divine and the demonic traits present in human nature. After mentioning twenty-six godly qualities, Krishna explains the demoniac nature which degrades the soul through arrogant, ignorant, and conceited pursuits of sense gratification and power (16:1-18).
He counsels that, in order to attain the supreme destination, one must give up lust, anger and greed (16:21-22).
Assisted by buddhi (intellect) and supported by scriptural confirmation, one should discern right and wrong and act appropriately (16:24).
Srimad Bhagavatam
Srimad Bhagavata Purana_The ripened fruit of all Vedas
In the great Naimasharaya forest, in the assembly of the exalted rishis headed by Rishi Śaunaka, the perfect one – Śrī Sūta Gosvāmi recited to them – the Bhāgavat Purāna as sung by Śrī Sukadeva Goswami to Maharaj Parikshit on the banks of Ma Ganga near Hastinapur, amidst the greatest confluence of enlightened sages from all over the universe. Śrī Sukadeva, a self-realized perfect soul – an Avadhuta, narrated the greatest of purana when only 16 years old, but more learned than all the realized rishis present. Śrī Sukadeva studied the Bhāgavatam through upanishad from his father, compiler of Śrīmad-Bhāgavatam – Bhagvān Veda Vyāsadeva (Bādarayana), Lord’s avataar.
Sukadev Goswami narrates Bhagavatam at the end of Dwaparayuga
idaṁ bhāgavataṁ nāma
purāṇaṁ brahma-sammitam
adhītavān dvāparādau
pitur dvaipāyanād aham
“At the end of the Dvāpara-yuga, I studied this great supplement of Vedic literature named Śrīmad-Bhāgavatam, which is equal to all the Vedas, from my father, Śrīla Dvaipāyana Vyāsadeva.”
~ Śrī Sukadeva Goswami, Śrīmad-Bhāgavatam (2.1.8)
Suta Goswami narrates Bhagavatam at Naimasharanya headed by Sunaka Rishi
idaṁ bhāgavataṁ nāma
purāṇaṁ brahma-sammitam
uttama-śloka-caritaṁ
cakāra bhagavān ṛṣiḥ
niḥśreyasāya lokasya
dhanyaṁ svasty-ayanaṁ mahat
This (idaṁ) scripture (purāṇaṁ) named (nāma) Śrīmad-Bhāgavatam (bhāgavataṁ ) is the literary incarnation (brahma-sammitam) of God (uttama-śloka-caritaṁ), and it is compiled (cakāra) by Śrīla Vyāsadeva (ṛṣiḥ), the incarnation of God (bhagavān). It is meant for the ultimate good (niḥśreyasāya) of all people (lokasya), and it is all-successful (dhanyaṁ), all-blissful (svasty-ayanaṁ) and all-perfect (mahat).
~ Sūta Gosvāmi in Śrīmad-Bhāgavatam (1.3.40)
As God is all light, all bliss and all perfection, so also is Śrīmad-Bhāgavatam. One can derive from its study all benefits that are possible to be derived from the personal presence of the Lord. It carries with it all the transcendental blessings of Lord Śrī Kṛṣṇa that we can expect from His personal contact. Śrīmad-Bhāgavatam should be worshiped as respectfully as we worship the Lord.
Spotless, Unlimited, Lord Krsna’s Representative!
Lord Śrī Caitanya Mahāprabhu has declared that Śrīmad-Bhāgavatam is the spotless (amalam) representation of all Vedic knowledge and history. What can be said more about this maha-purana? It is directly the Lord Himself – Krishna, Uttama-śloka in His absolute literary incarnation.
Mahaprabhu while giving confidential instructions to Sanatana Goswami says – “Just try to understand the real nature of Srimad-Bhagavatam. It is the sound representation of the Supreme Lord Krishna; therefore Srimad-Bhagavatam is not different from Krishna. Krishna is unlimited,
Bhagvad gita chapter 17 , (Revised 2021) The flowcharts and overview Medicherla Kumar
Revised and updated in 2021.
Bhagvad gita chapter 17 , The flowcharts and overview black and white for printing.
Chapter 17: Sraddhatraya-Vibhaga Yoga (The yoga of the classification of the threefold faith) - 28 verses. Krishna mentions three divisions of beliefs, thoughts, deeds and even eating habits corresponding to the three gunas (17:4-10).
• ,
• Faith in Gita ,
Shraddha or Faith
The flowcharts and overview
BHAKTI YOGA By
Dr. Pankaj Shukla
(AYURVEDACHARYA)
ॐ सह नाववतु।
सह नौ भुनक्तु।
सह वीर्यंकरवावहै।
तेजस्वि नावधीतमस्तुमा विद्विषावहै।
ॐ शान्तिः शान्तिः शान्तिः
What is Bhakti ?
Types of Bhakti
How to Cultivate Bhakti
Bhavas in Bhakti
Nava-Vidha-Bhakti
Fruits of Bhakti
Author : HH RP Bhakti Raghava Swami
E-Mail : Bhakti.Raghava.Swami@pamho.net
Date Produced : September 29, 2010
Editor : Srimati Vrinadavan Lila d.d.
Serial No : 8 of 54
Author : HH RP Bhakti Raghava Swami
E-Mail : Bhakti.Raghava.Swami@pamho.net
Date Produced : February 2009
Editor : Srimati Vrindavana Lila d.d.
Serial No. : 36 of 54
Author : Sriman Krishna Balarama Das
Email : kbd@sscclab.com
Date Produced : February 12, 2015
Editor : Sriman Subhananda Hari Das
Serial no : 38 of 54
Report at Rural Development Conference sponsored by
National Institute for Rural Development – India
Author : Sriman Hari Kirtan das
Email : hkd75@yahoo.com
Date Produced : November, 2016
Editor : HH RP Bhakti Raghava Swami
Serial No. : 48 of 54
Author : Vrsabha das
Email : vrsabha@gmail.com
Date : January 3, 2010
Text editing : Lyn Welker (Labangalatika d.d.)
Edited by : Sriman Subhananda Hari Das
Serial No. : 17 of 54
Most of the text was taken from Stephen Knapp
www.stephen-knapp.com
Table of Contents Title PageWELCOMETHE VAJRA.docxdeanmtaylor1545
Table of Contents
Title Page
WELCOME
THE VAJRACCHEDIKA PRAJÑAPARAMITA SUTRA
COMMENTARIES
PART ONE - THE DIALECTICS OF
PRAJÑAPARAMITA
Chapter 1 - THE SETTING
Chapter 2 - SUBHUTI’S QUESTION
Chapter 3 - THE FIRST FLASH OF LIGHTNING
Chapter 4 - THE GREATEST GIFT
Chapter 5 - SIGNLESSNESS
PART TWO - THE LANGUAGE OF
NONATTACHMENT
Chapter 6 - A ROSE IS NOT A ROSE
Chapter 7 - ENTERING THE OCEAN OF REALITY
Chapter 8 - NONATTACHMENT
PART THREE - THE ANSWER IS IN
THE QUESTION
Chapter 9 - DWELLING IN PEACE
Chapter 10 - CREATING A FORMLESS PURE
LAND
Chapter 11 - THE SAND IN THE GANGES
Chapter 12 - EVERY LAND IS A HOLY LAND
Chapter 13 - THE DIAMOND THAT CUTS
THROUGH ILLUSION
Chapter 14 - ABIDING IN NON-ABIDING
Chapter 15 - GREAT DETERMINATION
Chapter 16 - THE LAST EPOCH
Chapter 17 - THE ANSWER IS IN THE QUESTION
PART FOUR - MOUNTAINS AND
RIVERS ARE OUR OWN BODY
Chapter 18 - REALITY IS A STEADILY FLOWING
STREAM
Chapter 19 - GREAT HAPPINESS
Chapter 20 - THIRTY-TWO MARKS
Chapter 21 - INSIGHT-LIFE
Chapter 22 - THE SUNFLOWER
Chapter 23 - THE MOON IS JUST THE MOON
Chapter 24 - THE MOST VIRTUOUS ACT
Chapter 25 - ORGANIC LOVE
Chapter 26 - A BASKET FILLED WITH WORDS
Chapter 27 - NOT CUT OFF FROM LIFE
Chapter 28 - VIRTUE AND HAPPINESS
Chapter 29 - NEITHER COMING NOR GOING
Chapter 30 - THE INDESCRIBABLE NATURE OF
ALL THINGS
Chapter 31 - TORTOISE HAIR AND RABBIT
HORNS
Chapter 32 - TEACHING THE DHARMA
CONCLUSION
Copyright Page
WELCOME
WELCOME
BROTHERS AND SISTERS, please read The Diamond
That Cuts through Illusion with a serene mind, a mind
free from views. It’s the basic sutra for the practice of
meditation. Late at night, it’s a pleasure to recite the
Diamond Sutra alone, in complete silence. The sutra is
so deep and wonderful. It has its own language. The
first Western scholars who obtained the text thought it
was talking nonsense. Its language seems mysterious,
but when you look deeply, you can understand.
Don’t rush into the commentaries or you may be
unduly influenced by them. Please read the sutra first.
You may see things that no commentator has seen. You
can read as if you were chanting, using your clear body
and mind to be in touch with the words. Try to
understand the sutra from your own experiences and
your own suffering. It is helpful to ask, “Do these
teachings of the Buddha have anything to do with my
daily life?” Abstract ideas can be beautiful, but if they
have nothing to do with our life, of what use are they?
So please ask, “Do the words have anything to do with
eating a meal, drinking tea, cutting wood, or carrying
water?”
The sutra’s full name is The Diamond That Cuts
through Illusion, Vajracchedika Prajñaparamita in
Sanskrit. Vajracchedika means “the diamond that cuts
through afflictions, ignorance, delusion, or illusion.” In
China and Vietnam, people generally call it the Diamond
Sutra, emphasizing the word “diamond,” but, in fact,
the phrase “cutting through” is the most important.
Prajñaparamita means “per.
Table of Contents Title PageWELCOMETHE VAJRA.docxperryk1
Table of Contents
Title Page
WELCOME
THE VAJRACCHEDIKA PRAJÑAPARAMITA SUTRA
COMMENTARIES
PART ONE - THE DIALECTICS OF
PRAJÑAPARAMITA
Chapter 1 - THE SETTING
Chapter 2 - SUBHUTI’S QUESTION
Chapter 3 - THE FIRST FLASH OF LIGHTNING
Chapter 4 - THE GREATEST GIFT
Chapter 5 - SIGNLESSNESS
PART TWO - THE LANGUAGE OF
NONATTACHMENT
Chapter 6 - A ROSE IS NOT A ROSE
Chapter 7 - ENTERING THE OCEAN OF REALITY
Chapter 8 - NONATTACHMENT
PART THREE - THE ANSWER IS IN
THE QUESTION
Chapter 9 - DWELLING IN PEACE
Chapter 10 - CREATING A FORMLESS PURE
LAND
Chapter 11 - THE SAND IN THE GANGES
Chapter 12 - EVERY LAND IS A HOLY LAND
Chapter 13 - THE DIAMOND THAT CUTS
THROUGH ILLUSION
Chapter 14 - ABIDING IN NON-ABIDING
Chapter 15 - GREAT DETERMINATION
Chapter 16 - THE LAST EPOCH
Chapter 17 - THE ANSWER IS IN THE QUESTION
PART FOUR - MOUNTAINS AND
RIVERS ARE OUR OWN BODY
Chapter 18 - REALITY IS A STEADILY FLOWING
STREAM
Chapter 19 - GREAT HAPPINESS
Chapter 20 - THIRTY-TWO MARKS
Chapter 21 - INSIGHT-LIFE
Chapter 22 - THE SUNFLOWER
Chapter 23 - THE MOON IS JUST THE MOON
Chapter 24 - THE MOST VIRTUOUS ACT
Chapter 25 - ORGANIC LOVE
Chapter 26 - A BASKET FILLED WITH WORDS
Chapter 27 - NOT CUT OFF FROM LIFE
Chapter 28 - VIRTUE AND HAPPINESS
Chapter 29 - NEITHER COMING NOR GOING
Chapter 30 - THE INDESCRIBABLE NATURE OF
ALL THINGS
Chapter 31 - TORTOISE HAIR AND RABBIT
HORNS
Chapter 32 - TEACHING THE DHARMA
CONCLUSION
Copyright Page
WELCOME
WELCOME
BROTHERS AND SISTERS, please read The Diamond
That Cuts through Illusion with a serene mind, a mind
free from views. It’s the basic sutra for the practice of
meditation. Late at night, it’s a pleasure to recite the
Diamond Sutra alone, in complete silence. The sutra is
so deep and wonderful. It has its own language. The
first Western scholars who obtained the text thought it
was talking nonsense. Its language seems mysterious,
but when you look deeply, you can understand.
Don’t rush into the commentaries or you may be
unduly influenced by them. Please read the sutra first.
You may see things that no commentator has seen. You
can read as if you were chanting, using your clear body
and mind to be in touch with the words. Try to
understand the sutra from your own experiences and
your own suffering. It is helpful to ask, “Do these
teachings of the Buddha have anything to do with my
daily life?” Abstract ideas can be beautiful, but if they
have nothing to do with our life, of what use are they?
So please ask, “Do the words have anything to do with
eating a meal, drinking tea, cutting wood, or carrying
water?”
The sutra’s full name is The Diamond That Cuts
through Illusion, Vajracchedika Prajñaparamita in
Sanskrit. Vajracchedika means “the diamond that cuts
through afflictions, ignorance, delusion, or illusion.” In
China and Vietnam, people generally call it the Diamond
Sutra, emphasizing the word “diamond,” but, in fact,
the phrase “cutting through” is the most important.
Prajñaparamita means “per.
Krishna Voice is an English monthly newsletter published by ISKCON Bangalore. One of the objectives of our temple is to increase Krishna Consciousness in the community. Through this newsletter, we want to promulgate the message of the Vedic scriptures like Bhagavad-gita and Srimad-Bhagavatam.
Krishna Voice Magazine is a English publication circulated on a Monthly basis. Download latest Krishna issue voice from https://www.iskconbangalore.org
Krishna Voice is an English monthly newsletter published by ISKCON Bangalore. One of the objectives of our temple is to increase Krishna Consciousness in the community. Through this newsletter, we want to promulgate the message of the Vedic scriptures like Bhagavad-gita and Srimad-Bhagavatam.
Vedic eco village western canada development plan 2017 26 SriSurabhi
We need more people to expand the eco village. New guest facilities will attract workers for infrastructure building & farming.
Eco Village plots to develop this year are Go Shala foundation (for future cows), gardens, kitchen, Patha Shala (Learning Center), Yoga Shala, and Yajna Shala
Author : Vrsabha das
Email : vrsabha@gmail.com
Date : April 2020
Text editing : Lyn Welker (Labangalatika d.d.)
Edited by : Sriman Subhananda Hari Das
Serial No. : 12 of 54
Most of the text was taken from Stephen Knapp
www.stephen-knapp.com
Week # 22 PPT
The presentation explains different ways in which city people can get involved in cow protection at individual, family or community level. It encourages the city folks to use cow based products and avoid using products derived from cow slaughter. It encourages people/devotees to associate with cows on a regular basis. It also touches environmental aspect by discouraging people to use plastic bags and living a simple life in self sufficient community. It offers natural solution to ecological problem through large scale grazing of cows. And finally it urges citizen to request Government to formulate laws for protection of Cows and its progeny. Effort has been made to communicate the message to viewers in brief and attractive way.
Observation of Io’s Resurfacing via Plume Deposition Using Ground-based Adapt...Sérgio Sacani
Since volcanic activity was first discovered on Io from Voyager images in 1979, changes
on Io’s surface have been monitored from both spacecraft and ground-based telescopes.
Here, we present the highest spatial resolution images of Io ever obtained from a groundbased telescope. These images, acquired by the SHARK-VIS instrument on the Large
Binocular Telescope, show evidence of a major resurfacing event on Io’s trailing hemisphere. When compared to the most recent spacecraft images, the SHARK-VIS images
show that a plume deposit from a powerful eruption at Pillan Patera has covered part
of the long-lived Pele plume deposit. Although this type of resurfacing event may be common on Io, few have been detected due to the rarity of spacecraft visits and the previously low spatial resolution available from Earth-based telescopes. The SHARK-VIS instrument ushers in a new era of high resolution imaging of Io’s surface using adaptive
optics at visible wavelengths.
(May 29th, 2024) Advancements in Intravital Microscopy- Insights for Preclini...Scintica Instrumentation
Intravital microscopy (IVM) is a powerful tool utilized to study cellular behavior over time and space in vivo. Much of our understanding of cell biology has been accomplished using various in vitro and ex vivo methods; however, these studies do not necessarily reflect the natural dynamics of biological processes. Unlike traditional cell culture or fixed tissue imaging, IVM allows for the ultra-fast high-resolution imaging of cellular processes over time and space and were studied in its natural environment. Real-time visualization of biological processes in the context of an intact organism helps maintain physiological relevance and provide insights into the progression of disease, response to treatments or developmental processes.
In this webinar we give an overview of advanced applications of the IVM system in preclinical research. IVIM technology is a provider of all-in-one intravital microscopy systems and solutions optimized for in vivo imaging of live animal models at sub-micron resolution. The system’s unique features and user-friendly software enables researchers to probe fast dynamic biological processes such as immune cell tracking, cell-cell interaction as well as vascularization and tumor metastasis with exceptional detail. This webinar will also give an overview of IVM being utilized in drug development, offering a view into the intricate interaction between drugs/nanoparticles and tissues in vivo and allows for the evaluation of therapeutic intervention in a variety of tissues and organs. This interdisciplinary collaboration continues to drive the advancements of novel therapeutic strategies.
Introduction:
RNA interference (RNAi) or Post-Transcriptional Gene Silencing (PTGS) is an important biological process for modulating eukaryotic gene expression.
It is highly conserved process of posttranscriptional gene silencing by which double stranded RNA (dsRNA) causes sequence-specific degradation of mRNA sequences.
dsRNA-induced gene silencing (RNAi) is reported in a wide range of eukaryotes ranging from worms, insects, mammals and plants.
This process mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, and regulates the expression of protein-coding genes.
What are small ncRNAs?
micro RNA (miRNA)
short interfering RNA (siRNA)
Properties of small non-coding RNA:
Involved in silencing mRNA transcripts.
Called “small” because they are usually only about 21-24 nucleotides long.
Synthesized by first cutting up longer precursor sequences (like the 61nt one that Lee discovered).
Silence an mRNA by base pairing with some sequence on the mRNA.
Discovery of siRNA?
The first small RNA:
In 1993 Rosalind Lee (Victor Ambros lab) was studying a non- coding gene in C. elegans, lin-4, that was involved in silencing of another gene, lin-14, at the appropriate time in the
development of the worm C. elegans.
Two small transcripts of lin-4 (22nt and 61nt) were found to be complementary to a sequence in the 3' UTR of lin-14.
Because lin-4 encoded no protein, she deduced that it must be these transcripts that are causing the silencing by RNA-RNA interactions.
Types of RNAi ( non coding RNA)
MiRNA
Length (23-25 nt)
Trans acting
Binds with target MRNA in mismatch
Translation inhibition
Si RNA
Length 21 nt.
Cis acting
Bind with target Mrna in perfect complementary sequence
Piwi-RNA
Length ; 25 to 36 nt.
Expressed in Germ Cells
Regulates trnasposomes activity
MECHANISM OF RNAI:
First the double-stranded RNA teams up with a protein complex named Dicer, which cuts the long RNA into short pieces.
Then another protein complex called RISC (RNA-induced silencing complex) discards one of the two RNA strands.
The RISC-docked, single-stranded RNA then pairs with the homologous mRNA and destroys it.
THE RISC COMPLEX:
RISC is large(>500kD) RNA multi- protein Binding complex which triggers MRNA degradation in response to MRNA
Unwinding of double stranded Si RNA by ATP independent Helicase
Active component of RISC is Ago proteins( ENDONUCLEASE) which cleave target MRNA.
DICER: endonuclease (RNase Family III)
Argonaute: Central Component of the RNA-Induced Silencing Complex (RISC)
One strand of the dsRNA produced by Dicer is retained in the RISC complex in association with Argonaute
ARGONAUTE PROTEIN :
1.PAZ(PIWI/Argonaute/ Zwille)- Recognition of target MRNA
2.PIWI (p-element induced wimpy Testis)- breaks Phosphodiester bond of mRNA.)RNAse H activity.
MiRNA:
The Double-stranded RNAs are naturally produced in eukaryotic cells during development, and they have a key role in regulating gene expression .
This presentation explores a brief idea about the structural and functional attributes of nucleotides, the structure and function of genetic materials along with the impact of UV rays and pH upon them.
Nutraceutical market, scope and growth: Herbal drug technologyLokesh Patil
As consumer awareness of health and wellness rises, the nutraceutical market—which includes goods like functional meals, drinks, and dietary supplements that provide health advantages beyond basic nutrition—is growing significantly. As healthcare expenses rise, the population ages, and people want natural and preventative health solutions more and more, this industry is increasing quickly. Further driving market expansion are product formulation innovations and the use of cutting-edge technology for customized nutrition. With its worldwide reach, the nutraceutical industry is expected to keep growing and provide significant chances for research and investment in a number of categories, including vitamins, minerals, probiotics, and herbal supplements.
Slide 1: Title Slide
Extrachromosomal Inheritance
Slide 2: Introduction to Extrachromosomal Inheritance
Definition: Extrachromosomal inheritance refers to the transmission of genetic material that is not found within the nucleus.
Key Components: Involves genes located in mitochondria, chloroplasts, and plasmids.
Slide 3: Mitochondrial Inheritance
Mitochondria: Organelles responsible for energy production.
Mitochondrial DNA (mtDNA): Circular DNA molecule found in mitochondria.
Inheritance Pattern: Maternally inherited, meaning it is passed from mothers to all their offspring.
Diseases: Examples include Leber’s hereditary optic neuropathy (LHON) and mitochondrial myopathy.
Slide 4: Chloroplast Inheritance
Chloroplasts: Organelles responsible for photosynthesis in plants.
Chloroplast DNA (cpDNA): Circular DNA molecule found in chloroplasts.
Inheritance Pattern: Often maternally inherited in most plants, but can vary in some species.
Examples: Variegation in plants, where leaf color patterns are determined by chloroplast DNA.
Slide 5: Plasmid Inheritance
Plasmids: Small, circular DNA molecules found in bacteria and some eukaryotes.
Features: Can carry antibiotic resistance genes and can be transferred between cells through processes like conjugation.
Significance: Important in biotechnology for gene cloning and genetic engineering.
Slide 6: Mechanisms of Extrachromosomal Inheritance
Non-Mendelian Patterns: Do not follow Mendel’s laws of inheritance.
Cytoplasmic Segregation: During cell division, organelles like mitochondria and chloroplasts are randomly distributed to daughter cells.
Heteroplasmy: Presence of more than one type of organellar genome within a cell, leading to variation in expression.
Slide 7: Examples of Extrachromosomal Inheritance
Four O’clock Plant (Mirabilis jalapa): Shows variegated leaves due to different cpDNA in leaf cells.
Petite Mutants in Yeast: Result from mutations in mitochondrial DNA affecting respiration.
Slide 8: Importance of Extrachromosomal Inheritance
Evolution: Provides insight into the evolution of eukaryotic cells.
Medicine: Understanding mitochondrial inheritance helps in diagnosing and treating mitochondrial diseases.
Agriculture: Chloroplast inheritance can be used in plant breeding and genetic modification.
Slide 9: Recent Research and Advances
Gene Editing: Techniques like CRISPR-Cas9 are being used to edit mitochondrial and chloroplast DNA.
Therapies: Development of mitochondrial replacement therapy (MRT) for preventing mitochondrial diseases.
Slide 10: Conclusion
Summary: Extrachromosomal inheritance involves the transmission of genetic material outside the nucleus and plays a crucial role in genetics, medicine, and biotechnology.
Future Directions: Continued research and technological advancements hold promise for new treatments and applications.
Slide 11: Questions and Discussion
Invite Audience: Open the floor for any questions or further discussion on the topic.
Earliest Galaxies in the JADES Origins Field: Luminosity Function and Cosmic ...Sérgio Sacani
We characterize the earliest galaxy population in the JADES Origins Field (JOF), the deepest
imaging field observed with JWST. We make use of the ancillary Hubble optical images (5 filters
spanning 0.4−0.9µm) and novel JWST images with 14 filters spanning 0.8−5µm, including 7 mediumband filters, and reaching total exposure times of up to 46 hours per filter. We combine all our data
at > 2.3µm to construct an ultradeep image, reaching as deep as ≈ 31.4 AB mag in the stack and
30.3-31.0 AB mag (5σ, r = 0.1” circular aperture) in individual filters. We measure photometric
redshifts and use robust selection criteria to identify a sample of eight galaxy candidates at redshifts
z = 11.5 − 15. These objects show compact half-light radii of R1/2 ∼ 50 − 200pc, stellar masses of
M⋆ ∼ 107−108M⊙, and star-formation rates of SFR ∼ 0.1−1 M⊙ yr−1
. Our search finds no candidates
at 15 < z < 20, placing upper limits at these redshifts. We develop a forward modeling approach to
infer the properties of the evolving luminosity function without binning in redshift or luminosity that
marginalizes over the photometric redshift uncertainty of our candidate galaxies and incorporates the
impact of non-detections. We find a z = 12 luminosity function in good agreement with prior results,
and that the luminosity function normalization and UV luminosity density decline by a factor of ∼ 2.5
from z = 12 to z = 14. We discuss the possible implications of our results in the context of theoretical
models for evolution of the dark matter halo mass function.
The Science of Daiva Varnasrama - Need of Varnasrama Dharma
1.
2. The Science of Daiva Varnasrama –
Need of Varnasrama Dharma
Author : HH RP Bhakti Raghava Swami (ACBSP)
E-mail : Bhakti.Raghava.Swami@pamho.net
Date Produced : 2011
Serial No : 14 of 54
3. Hare Krishna.
The following presentation was prepared based on
Part II of the book
‘ The Science of Daiva Varnasrama ’
Author: RP Bhakti Raghava Swami (ACBSP).
5. Hare Krishna.
In the previous presentations we have discussed about understanding
Varnasrama Dharma.
We discussed about Dharma, Cow protection, Agriculture, Sadhana
Bhakti, Sambandha-Abhideya-Prajoyana, Chaitanya Mahaprabhu on
Varnasrama and Srila Prabhupada’s vision etc.
In this presentation we will quickly have a short discussion on the
Need of Varnasrama Dharma …
…. Recap
6. Chanting will go on... But at the same time the varnasrama-dharma
must be established to make the way easy… Chaitanya
Mahaprabhu recommended, sthane sthitah. Therefore
varnasrama-dharma is required. Simply show-bottle will not do.
So the varnasrama-dharma should be introduced all over the
world.
[Room conversation in Mayapur, February 14, 1977]
7. What is the importance of scientific
social structure?
• The whole Vedic culture, in this way, is extremely practical. It
takes into consideration the reality of the material dimension as
well as the spiritual dimension of life. In varnasrama,
the varëas are dealing with the material dimension in terms of
how to deal with the material energy, how to engage ourselves,
and what kind of occupation to take-up, etc. The äçramas are
especially concentrating on the spiritual dimension of life. So in
this way, there is a proper balance, a perfect harmony. Other
systems just fall short of the goal. That is why Srila Prabhupäda
makes this very strong statement that– “no other political,
economical system, etc, can replace this system of varëa and
äçrama” [Room Conversation, October 18, 1977, Vrndavana].
8. “First is Food”, “First is Character” – Why
such statements by Srila Prabhupäda?
• On a number of occasions, Srila Prabhupäda did mention “First is Food” and
also “First is Character”. Quite significant statements! What is the meaning of
“First is Food” in the context of varnasrama? In the Vedic culture, everyone
was growing their own food and in this way people would live locally and be
closely connected with both nature and one another. This way to live is simply
something very practical. Unless we have food, we cannot perform any
activity. So, “First is Food”.
• In the Bhagavad-gétä in the context of yajïa. One should
perform yajïa. Yajïa means one should take up one’s natural prescribed
duties. And one of those activities is in connection with following the natural
system that Kåñëa has given whereby we are working in harmony with nature
and whereby we are producing our own food, by properly using the land. And
even to perform any fire sacrifice, the brähmaëas need grains, they
need ghee. If no one is cultivating, if no one is keeping cows, where will the
grains and the ghee come from?
9. What is the need to divide the
society?
• “The natural divisions are described in the Bhagavad-gétä and
Lord Krsna explains that He has created this system of varëa
and āśrama, so of course that's another reason why we should
introduce varnasrama. It is a natural system that has been
created by the Supreme Personality of Godhead, Krsna Himself,
to help us properly function even in the material world. What is
important to understand is that it not only helps us to function
better materially, but also helps us to progress spiritually when
we adopt the system of varëa and āśrama. There is a statement
given by one of our foremost äcäryas, Srila Bhaktivinoda
Thakura. He explains that the best way one can advance in
spiritual life, as a Vaiñëava, as a devotee, is by remaining and
working within the system of varëa and āśrama. So, when it is
so clearly explained and defined by our çästras, by our äcäryas,
who are we to change this?”
10. What is the actual purpose of
Varnasrama ?
• The whole purpose of varnasrama is to form an ideal society
where everyone can more easily advance in spiritual life. We
find this described in the Srimad- Bhagavatam, Canto 7,
Chapters 11 through 15, dealing with “Ideal Society, Ideal Family
Life and Perfect Society”.
• So it is simple and very logical to understand. There is a material
system given by the Supreme Personality of Godhead. That
system is meant to facilitate individuals to live in harmony within
society based on real values in life. Self-realization in the Vedic
culture is the primary goal and objective of life.
• In other words, varnasrama harmonizes both the spiritual and
material dimensions of life very nicely. So when we introduce the
system of varnasrama it is authorized, it is scientific, it is
practical, it is natural, it is the norm.
11. Does varnasrama exist today? If so,
what is the need to re-establish it?
• When we understand the basic principle behind varnasrama
dharma, then in one sense varnasrama dharma always exists.
There is always a class of people who are intellectual. There is
always a class of people administrating, organizing, etc. The
four varëas – brähmana, kshatriya, vaisya and südra always
exist. But they are covered and not properly understood and
those activities are not performed according to the norms
established within the Vedic literatures. So there is a need …in
our modern society we have what is called…Srila Prabhupäda
refers to as varna sankara. Varnaa sankara is a special
characteristic of Kali Yuga where everything becomes very
‘mixed’. One cannot understand or differentiate things or people;
most people also have a very hard time understanding what they
should be doing, what is their actual duty and occupation in life.
Most people just don’t know, even amongst our own devotees.
12. Hare Krishna Hare Krishna
Krishna Krishna Hare Hare
Hare Rama Hare Rama
Rama Rama Hare Hare
13. ISKCON Daiva Varnasrama Ministry
Promoting Rural Development in
India
www.iskconvarnasrama.com
OM Sri Surabhi Campaign
www.srisurabhi.org
14.
15. ABOUT THE AUTHOR
Name HH RP Bhakti Raghava Swami
Age and Gender 71, Male
Country/Province of Origin Canada, Ontario
Educational Degree and Year B.A, 1968, M.Ed., 2004
Educational Institution State University of Yogyakarta
Professional Work/Experience Social Worker/Counselor
Affiliation with ISKCON Since 1974
Present Service in ISKCON Minister, IDVM-India