Molluscs form valuable fisheries in various parts of the coast of India providing shell fish as food and as source of lime, pearls and decorative shells, as constituents of medicinal preparations etc. In the present study, the eco-friendly Turbo brunneus and its vital role in future pharmaceutical industry through GC-MS analysis was carried out. Almost eighteen compounds are obtained through GC-MS which might be responsible for antimicrobial, pharmaceutical, insect repellent, anti-inflammatory, anticancer, antiasthmatic, diuretic and antiarthritic activities.
Design, synthesis, characterization and biological evaluation of 3- (4-(7-chl...iosrjce
Novel thiazolidinone derivatives TQ-VI(1-10) were designed, synthesized and screened for
antimicrobial activity. Synthesis of 3-(4-(7-chloro-2-(4-chlorophenyl) 4-oxo-quinazolin-3(4H)-yl) phenyl) -
2-arylthiazolidin-4-one TQ-VI(1-10) have been achieved from the starting material 2-amino-4-chlorobenzoic
acid TQ-I on cyclization with p-chlorobenzoyl chloride TQ-II to yield 7-chloro-2-(4-chlorophenyl)-4H-3,1-
benzoxazin-4-one,TQ-III, which on treatment with p-phenylindiamine gave 3-(4-aminophenyl)-7-chloro-2-(4-
chlorophenyl)quinazolin-4(3H)-one, TQ-IV in good yield. Then, TQ-IV on reaction with substituted aromatic
aldehydes converted to TQ-V(1-10), which on cyclization with thioglycolic acid gave TQ-VI (1-10). All the
synthesized compounds have been characterized on the basis of IR,
1
H-NMR and mass spectral data. The
compounds containing 4-OH, 4-OCH3 and 3,4,5-(OCH3)3 showed good activity. The title compounds were
screened for qualitative (zone of inhibition) and quantitative antimicrobial activity (MIC) by agar cup plate
method and serial dilution technique, respectively. Among the synthesized compounds in the series, the
compound TQVI4 and TQVI5 were found to exhibit significant antifungal activity at lower concentration of
31.25 µg/ml, against A.niger. The compound TQVI5 and TQVI4 showed zone of inhibition of 17mm and 15mm
against A.niger and C.albicans respectively which is comparable to that of standard drug. The rest of the
analogues in the series displayed weak to moderate antimicrobial activity when compared to the standard
positive controls Ampicillin and Amphotericin B.
Phytochemical Studies on Linum Usitatissimum Seeds and the Nanoformulation of...Editor IJCATR
The phytochemical investigation of Linum usitatissimum, family Linaceae, resulted in the separation and
identification of a lignane, 8–5' neolignan 1 (dehydrodiconiferyl alcohol-4-β-D-glucoside), in addition to
identification of many compounds by the GC/MS technique. The antimicrobial activities of hexane, methylene
chloride and butanol fractions were comparable to ampicillin. The activities against E. coli were 29.2%, 37.5%,
and 66.7%, respectively; against S. aureus were 45.4%, 36.4% and 63.6%, respectively, and against C. albicans
were 26.9%, 46.1% and 73.1%, respectively. Antioxidant activity was assessed by ABTS method. It decreased
in the following order: ascorbic acid > butanol fraction > methylene chloride fraction > hexane fraction. The
cytotoxicity against HePG2 was found to be “moderate” for butanol and methylene chloride fractions, and
“weak” for the hexane fraction. The cytotoxicity against MCF-7 was found to be “strong” for butanol fraction,
and “weak” for both hexane and methylene chloride fractions. The lignane-rich subfraction, Lu 3d was
incorporated into pluronic nano-micelles using nanoprecipitation technique through a modified procedure.
The physicochemical characteristics of the developed Lu 3d-loaded nano-micelles such as particle size,
potential and morphology were determined using DLS and HR-TEM. The average diameters of the prepared
plain and Lu 3d-loaded pluronic nano-micelles were found to be 207 ± 12 and 225 ± 18 nm, respectively. The
encapsulation of Lu 3d into nano-sized particles has enhanced their aqueous dissolution and consequently
improved their bioavailability. Nanoformulation of Lu 3d led also to a stable colloidal dispersion with a strong
green color, indicating its homogenous distribution in the aqueous medium at a significantly higher
concentration than that obtained using other solvents.
Phytochemical analysis, protein content & antimicrobial activities of sel...eSAT Journals
Abstract Two seed samples of Glycine max Linn. (S1, S2) were purchased from two retail stores of local market. Non-sprouted and sprouted seed powder were extracted separately with methanol (100%, 50%) by cold maceration to obtain methanolic and hydroalcoholic extract of Glycine max Sample 1 was designated as MES1 and HES1 and sample 2 as MES2 and HES2 respectively. Phytochemical analysis indicated the presence of various phytoconstituents viz. phytosterols, flavonoids, phenolic compounds, tannins, carbohydrates, proteins, amino acids, fixed oils and fats etc. Thin layer chromatography study on extracts revealed the presence of a number of compounds. The protein content of these samples were studied. The protein content of samples MES1, HES1, MES2 and HES2 with respect to BSA was found to be 90.6 2μg/ml, 82μg/ml, 94.5μg/ml and 79.1μg/ml respectively. The highest among these were found to be in MES2. Sprouting enhanced the protein content of the two samples. The samples have shown antimicrobial activity at selected concentration and microbial strains (26mm) for gram negative bacteria (27mm) for gram positive bacteria. Keywords: Glycine max Linn, phytochemical constituents, TLC, antimicrobial activity, protein, methanolic extract, hydroalcoholic extract.
IJRET : International Journal of Research in Engineering and Technology is an international peer reviewed, online journal published by eSAT Publishing House for the enhancement of research in various disciplines of Engineering and Technology. The aim and scope of the journal is to provide an academic medium and an important reference for the advancement and dissemination of research results that support high-level learning, teaching and research in the fields of Engineering and Technology. We bring together Scientists, Academician, Field Engineers, Scholars and Students of related fields of Engineering and Technology
Design, synthesis, characterization and biological evaluation of 3- (4-(7-chl...iosrjce
Novel thiazolidinone derivatives TQ-VI(1-10) were designed, synthesized and screened for
antimicrobial activity. Synthesis of 3-(4-(7-chloro-2-(4-chlorophenyl) 4-oxo-quinazolin-3(4H)-yl) phenyl) -
2-arylthiazolidin-4-one TQ-VI(1-10) have been achieved from the starting material 2-amino-4-chlorobenzoic
acid TQ-I on cyclization with p-chlorobenzoyl chloride TQ-II to yield 7-chloro-2-(4-chlorophenyl)-4H-3,1-
benzoxazin-4-one,TQ-III, which on treatment with p-phenylindiamine gave 3-(4-aminophenyl)-7-chloro-2-(4-
chlorophenyl)quinazolin-4(3H)-one, TQ-IV in good yield. Then, TQ-IV on reaction with substituted aromatic
aldehydes converted to TQ-V(1-10), which on cyclization with thioglycolic acid gave TQ-VI (1-10). All the
synthesized compounds have been characterized on the basis of IR,
1
H-NMR and mass spectral data. The
compounds containing 4-OH, 4-OCH3 and 3,4,5-(OCH3)3 showed good activity. The title compounds were
screened for qualitative (zone of inhibition) and quantitative antimicrobial activity (MIC) by agar cup plate
method and serial dilution technique, respectively. Among the synthesized compounds in the series, the
compound TQVI4 and TQVI5 were found to exhibit significant antifungal activity at lower concentration of
31.25 µg/ml, against A.niger. The compound TQVI5 and TQVI4 showed zone of inhibition of 17mm and 15mm
against A.niger and C.albicans respectively which is comparable to that of standard drug. The rest of the
analogues in the series displayed weak to moderate antimicrobial activity when compared to the standard
positive controls Ampicillin and Amphotericin B.
Phytochemical Studies on Linum Usitatissimum Seeds and the Nanoformulation of...Editor IJCATR
The phytochemical investigation of Linum usitatissimum, family Linaceae, resulted in the separation and
identification of a lignane, 8–5' neolignan 1 (dehydrodiconiferyl alcohol-4-β-D-glucoside), in addition to
identification of many compounds by the GC/MS technique. The antimicrobial activities of hexane, methylene
chloride and butanol fractions were comparable to ampicillin. The activities against E. coli were 29.2%, 37.5%,
and 66.7%, respectively; against S. aureus were 45.4%, 36.4% and 63.6%, respectively, and against C. albicans
were 26.9%, 46.1% and 73.1%, respectively. Antioxidant activity was assessed by ABTS method. It decreased
in the following order: ascorbic acid > butanol fraction > methylene chloride fraction > hexane fraction. The
cytotoxicity against HePG2 was found to be “moderate” for butanol and methylene chloride fractions, and
“weak” for the hexane fraction. The cytotoxicity against MCF-7 was found to be “strong” for butanol fraction,
and “weak” for both hexane and methylene chloride fractions. The lignane-rich subfraction, Lu 3d was
incorporated into pluronic nano-micelles using nanoprecipitation technique through a modified procedure.
The physicochemical characteristics of the developed Lu 3d-loaded nano-micelles such as particle size,
potential and morphology were determined using DLS and HR-TEM. The average diameters of the prepared
plain and Lu 3d-loaded pluronic nano-micelles were found to be 207 ± 12 and 225 ± 18 nm, respectively. The
encapsulation of Lu 3d into nano-sized particles has enhanced their aqueous dissolution and consequently
improved their bioavailability. Nanoformulation of Lu 3d led also to a stable colloidal dispersion with a strong
green color, indicating its homogenous distribution in the aqueous medium at a significantly higher
concentration than that obtained using other solvents.
Phytochemical analysis, protein content & antimicrobial activities of sel...eSAT Journals
Abstract Two seed samples of Glycine max Linn. (S1, S2) were purchased from two retail stores of local market. Non-sprouted and sprouted seed powder were extracted separately with methanol (100%, 50%) by cold maceration to obtain methanolic and hydroalcoholic extract of Glycine max Sample 1 was designated as MES1 and HES1 and sample 2 as MES2 and HES2 respectively. Phytochemical analysis indicated the presence of various phytoconstituents viz. phytosterols, flavonoids, phenolic compounds, tannins, carbohydrates, proteins, amino acids, fixed oils and fats etc. Thin layer chromatography study on extracts revealed the presence of a number of compounds. The protein content of these samples were studied. The protein content of samples MES1, HES1, MES2 and HES2 with respect to BSA was found to be 90.6 2μg/ml, 82μg/ml, 94.5μg/ml and 79.1μg/ml respectively. The highest among these were found to be in MES2. Sprouting enhanced the protein content of the two samples. The samples have shown antimicrobial activity at selected concentration and microbial strains (26mm) for gram negative bacteria (27mm) for gram positive bacteria. Keywords: Glycine max Linn, phytochemical constituents, TLC, antimicrobial activity, protein, methanolic extract, hydroalcoholic extract.
IJRET : International Journal of Research in Engineering and Technology is an international peer reviewed, online journal published by eSAT Publishing House for the enhancement of research in various disciplines of Engineering and Technology. The aim and scope of the journal is to provide an academic medium and an important reference for the advancement and dissemination of research results that support high-level learning, teaching and research in the fields of Engineering and Technology. We bring together Scientists, Academician, Field Engineers, Scholars and Students of related fields of Engineering and Technology
INVESTIGATION OF IN-VITRO ANTHELMINTIC AND CYTOTOXIC ACTIVITIES OF ARTABOTRYS...Syed Masudur Rahman Dewan
The methanolic extract of bark of Artabotrys hexapetalus were investigated for in-vitro anthelmintic and cytotoxic activities. Evaluation of cytotoxic activity was done using the brine shrimp lethality bio-assay. The crude methanolic extract showed significant cytotoxic potential (LC50 value of 7.688 μg/ml) comparing with that of standard vincristine (0.839 μg/ml). The other study was undertaken to evaluate anthelmintic activity where albendazole was used as reference standard. Methanolic extract of barks (50 mg/ml) caused paralysis of the worms at 68.33 minutes and death at 84.0 minutes while albendazole (positive control) paralyzed and killed the worms at 17 minutes and 48 minutes respectively at the concentration of 10 mg/ml. The study confirms the significant anthelmintic activities of bark extract of Artabotrys hexapetalus and therefore demands the isolation of active principles through bioassay.
Fatty Acid Pattern and Alkaloids of Echium RauwolfiiEditor IJCATR
The GC/MS analysis of hexane extract revealed the presence of palmitic acid as saturated fatty acid (1.05%), versus oleic acid (2.18%), linoleic acid (1.13%), cis-8,11,14-eicosatrienoic acid (2.12%) as unsaturated fatty acids. On the other hand, CH2Cl2 extract contained palmitic acid methyl ester (3.55%), and methyl isostearate (1.17%) as saturated fatty acids, versus linoleic acid methyl ester (3.57%) and linolenic acid methyl ester (10.01%) as unsaturated fatty acids. The GC/MS analysis of the alkaloid-rich fraction indicated the presence of the pyrazolidine alkaloids petranine (2.97%), 7-angeloyl-9-(2-methylbutyryl) retronecine (4.22%), 7-angeloylretronecine (0.59%) and 9-angeloylretronecine (0.47%).
The butanol extract showed the heights DPPH radical scavenging activity (IC50 = 14.3 µg),. while ethyl acetate extract was very weak in activity (IC50 = 432.3 µg) and no activity with hexane and methylene chloride extract.
The antimicrobial potentials of E. rauwolfii extracts were examined. The inhibition of the fungi species by ethyl acetate extract exert was comparable to Amphotericin B. The inhibition zone of the butanol extract against Streptococcus pneumonia was comparable to Ampicillin, against Pseudomonas aeruginosa was comparable to Gentamicin and Escherichia coli was comparable to Gentamicin.
The cytotoxicity against HePG-2 of ethyl acetate extract and butanol extract were “very strong”, and that of hexane extract and methylene chloride extract were “moderate”, against MCF-7 of ethyl acetate extract and butanol extract were “strong”, that of methylene chloride extract was “moderate”, and that of hexane extract was “weak” and against HCT-116 of butanol extract was “very strong”, of ethyl acetate extract was“strong”, of methylene chloride extract and hexane extract were “moderate”.
RESIDUAL ESTIMATION OF ISOPROTURON, ATRAZINE AND GRAIN PROTECTANTS IN STORED ...ijabjournal
This study was carried out to see the occurrence and level of pesticide residues in stored wheat grain samples. Wheat grains were collected from different godowns located in Faisalabad district. Weedicide residues in wheat grains were analyzed by using thin plate liquid chromatography and validated high
performance liquid chromatography. On the basis of extent of inhibition of the Hill reaction, it was found that the level of atrazine was (0-0.028 ngkg-1) and the level of isopproturon was (0-0.024 ngkg-1) in test samples. The calculated values were below the Maximum Residue Limit (MEL) i.e. 100 ngkg-1 for atrazine and 2200 ngkg-1 for isoproturon. It was found that presence of herbicide residues in stored wheat was negligible. Further, on wheat grain samples placed in jars and treated with deltamethrin and cypermethrin @0.4 and 0.8% concentration. The persistency data was calculated after 1 hour, 7, 15 and 30 days of treatment. The grain protectants were continuously degraded with the passage of time and after the 30 days
the amount of residues of the grain protectants was found to be very small. It was concluded that the use of the grain protectants in the powder form in combination with other botanicals on stored wheat samples is feasible to reduce the insect pest in stored grains.
Chemical Composition And Acridicid Properties Of The Moroccan Tanacetum Annuu...inventy
Research Inventy : International Journal of Engineering and Science is published by the group of young academic and industrial researchers with 12 Issues per year. It is an online as well as print version open access journal that provides rapid publication (monthly) of articles in all areas of the subject such as: civil, mechanical, chemical, electronic and computer engineering as well as production and information technology. The Journal welcomes the submission of manuscripts that meet the general criteria of significance and scientific excellence. Papers will be published by rapid process within 20 days after acceptance and peer review process takes only 7 days. All articles published in Research Inventy will be peer-reviewed.
COMPARATIVE STUDY OF THE ANTIFUNGAL EFFECT OF OILS AND THEIR UNSAPONIFIABLE F...EDITOR IJCRCPS
The main objective of the study was to assess the in vitro antifungal potency of the unsaponifiable fraction extracted
from coat and bark seeds oils of Citrullus colocynthis L against pathogenic fungal strains namely Aspergillus flavus,
Aspergillus ochraceus, Penicillium expansum and Fusarium oxysporum. In terms of the physico-chemical
characterization, oils under study showed evidence of quality standards relating to vegetable oils. Unsaponifiable
matter yield recorded was approximately 0.93% and 1.03%, for the seed coat oil and bark seed oil respectively.
Antifungal activity carried out by radial growth on solid medium (Potatoes Dextrose Agar acidified) revealed that the
oils and the corresponding unsaponifiable fractions exhibited complete inhibition of fungal growth. Maximal antifungal
index inhibition (IAF=100%) were recorded at 5% and 2.5% dilutions of each fraction tested. The results provided
evidence that the unsaponifiable oils fractions might indeed be potential sources of natural antifungal agents and
deserve further studies to characterize the biological compounds included in these fractions.
Keywords: Citrullus colocynthis seeds, seed coat, bark, oils, unsaponifiable fraction, antifungal activity.
Anti Bacterial and Anti Oxidant Activities of Evolvulus Alsinoides LinnIOSRJPBS
: Plants are very important sources of potential useful raw materials as natural chemotherapeutic agents. Shankhpushpi is botanically termed as Evolvulus alsinoides; the extracts have exhibited antioxidant, anti-ulcer, and immunomodulatory activities. The present work is to investigate the invitro therapeutic potential activities of methanolic extract of whole plant of Evolvulus alsinoides on the antimicrobial activity against five clinical pathogenic bacterial strains viz., Staphylococcus aureus, Bacillus cereus, Escherichia coli, Klebshiella pneumonia and Salmonella typhii, using agar well diffusion assay and Anti oxidant activity using 1-1-Diphenyl- 2-picryl-hydrazyl (DPPH) radical. Extract inhibited IC50: 40.2. Total phenolic content: 28.4 mg/ml & Total flavonoid content: 20.2 μg /ml. Evolvulus alsinoides (MIC): 512.5mg/ml possess 30 to 40 % inhibition, the antibacterial activity of the herbal extracts was more pronounced on the gram- negative bacteria Klebshiella pneumonia than the gram-positive bacteria Staphylococcus aureus. The free radical scavenging activity of methanolic activity of whole plant of Evolvulus alsinoides increase in a concentration dependent manner, and posses statistically significance DPPH free radical scavenging & Anti microbial activities
GC-MS Analysis and Evaluation of In-vitro Antioxidant Potential and Total Phe...Innspub Net
Wild hops (Flemingia strobilifera Linn.) is a shrub belonging to Fabaceae family. The leaves of F. strobilifera are commonly used by the Subanen, the indigenous group in Lapuyan, Zamboanga del Sur, Philippines to treat inflammation. In this study, the hydromethanolic (80%) and aqueous leaf extracts of F. strobilifera were evaluated for their antioxidant activity and total phenolics content. The active semi-volatile components of 80% methanol leaf extract were analyzed using Gas Chromatography-Mass Spectrometry (GC-MS). DPPH radical scavenging activity was used to determine the potential of this plant as anti-oxidant. Total phenolics content was determined using Folin-Ciocalteu reagent and calculated as gallic acid equivalence. GC-MS analysis revealed the presence of eight compounds. Both the 80% methanol and aqueous extracts of F. strobilifera showed significant scavenging activity with low IC50 values of 0.299 mg/mL and <0.25 mg/mL, respectively. There was positive correlation between the scavenging activity percentage and the total phenolics content only in the aqueous extract of F. strobilifera while 80% methanol extract showed negative correlation between inhibition percentage and total phenolics content which can be attributed to the solvent used and method used in quantification of phenolics. Nevertheless, the results suggest that these leaf extracts are potent source of antioxidant compounds and may serve as natural anti-inflammatory agents.
Biochemical and pharmacological study of biologically active preparation of p...inventionjournals
Our aim was to perform some biochemical and pharmacological studies of bioactive bovine placental preparation via digestion of cow placenta using enzyme contained in swine stomach. Amino acid compositions and contents in biologically active preparation of placenta, obtained by digestion of cow placenta with enzyme contained in swine stomach were measured by HPLC technique and it was found that contents of such amino acids as glycine, proline and lysine were highest and 9 essential amino acids, including valine, histidine, methionine, lysine, threonine, arginine, phenylalanine, leucine and isoleucine were measured. In pharmacological study, acute toxicity (LD50) of the preparation and effect of the preparation on immune response to sheep erythrocyte were investigated in white mice, weighing 18 to 20 g each. The study revealed acute toxicity (LD50) of the preparation was 60 ml per kg. Spleen index of the first and second experimental group animals treated by the preparation during both provoked and unprovoked immune responses increased by 1.2 to 3.09 times as compared to that of negative control animals, while splenocyte count elevated by 1.2 to 2.2 times than negative control animals. Higher contents of essential amino acids of the biologically active preparation of cattle placenta shows its biologically higher nutritive value, as well as pharmacological study reveals the preparation has minimal toxicity and higher effect to stimulate immune responses.
protease activity of extracellular enzyme produced by b. subtilis isolated fr...IJEAB
Background: Proteases produced by enzymatic method are more environments friendly than chemical process, and they have tremendous potential in the leather industry and in other several industries. In this study extracellular protease producing non pathogenic Bacillus subtilis was isolated from soil sample and relationship between sporulation and extracellular protease synthesis in large scale cultivation was studied. The enzyme was further characterized, purified, and tested for potential application. Result: The molecular weight of the protease was found to be ~30 KDa. Enzyme activity was checked on the presence of different metal ions and effectors. The enzyme was slightly modulated by MG++ ion, and significantly by Hg++ ion, while Zn++ ion slightly decrease the proteolytic activity. Sulfahydryl reagents, DTT slightly and β-ME significantly inhibit the enzyme. EDTA showed no effect on the enzyme suggesting that the enzyme might not be metalloprotease. PMSF, a known serine protease inhibitor was seen to totally inhibit the enzyme which indicates that the enzyme is a serine protease. The optimum enzyme activity was observed after 22 hours of incubation of B. subtilis at 37o C. Conclusions: Crude enzyme contains 285 units of enzyme which have direct dehairing activity. The enzyme was also seen to be able to remove blood and curry stain from clothes; making it a very promising candidate to be used in a leather and detergent industry. Apart from protease the bacterium was also seen to have lipase and collagenase activity. So, the bacteria are potentially good candidate for industrial application.
Novel Hybrid Molecules of Isoxazole Chalcone Derivatives: Synthesis and Study...Ratnakaram Venkata Nadh
medicine due to their significant role in the treatment of different health problems.
Methods: We have synthesized new series of isoxazole-chalcone conjugates (14a-m) by the
Claisen-Schmidt condensation of suitable substituted acetophenones with isoxazole aldehydes (12a-d).
In vitro cytotoxic activity of the synthesized compounds was studied against four different selected
human cancer cell lines by using sulforhodamine B (SRB) method.
Results: The adopted scheme resulted in good yields of new series of isoxazole-chalcone
conjugates (14a-m). Potent cytotoxic activity was observed for compounds -14a, 14b, 14e, 14i, 14j
and 14k against prostate DU-145 cancer cell line.
Conclusion: The observed potent cytotoxic activities were due to the presence of 3,4,5-
trimethoxyphenyl group.
Synthesis and Pharmacological Evaluation of Novel Heterocyclic CompoundIJSRD
2-[(4-bromo anilino)]-4-chloro-6-morpholino-1,3,5-triazine[2a] and 4-chloro-N-(4-fluorophenyl)-6-morpholino-1,3,5-triazin-2-amine [2b]were synthesized and studied for their biological activity. These compounds were prepared by the condensation of 4-bromoaniline and 4-fluoroaniline with 4-(4,6-dichloro-1,3,5-triazin-2-yl) morpholine [1] which is prepared by the reaction between 2,4,6-trichloro-1,3,5-triazine and morpholine. All the compounds were characterized by elemental analysis and spectral studies.
INVESTIGATION OF IN-VITRO ANTHELMINTIC AND CYTOTOXIC ACTIVITIES OF ARTABOTRYS...Syed Masudur Rahman Dewan
The methanolic extract of bark of Artabotrys hexapetalus were investigated for in-vitro anthelmintic and cytotoxic activities. Evaluation of cytotoxic activity was done using the brine shrimp lethality bio-assay. The crude methanolic extract showed significant cytotoxic potential (LC50 value of 7.688 μg/ml) comparing with that of standard vincristine (0.839 μg/ml). The other study was undertaken to evaluate anthelmintic activity where albendazole was used as reference standard. Methanolic extract of barks (50 mg/ml) caused paralysis of the worms at 68.33 minutes and death at 84.0 minutes while albendazole (positive control) paralyzed and killed the worms at 17 minutes and 48 minutes respectively at the concentration of 10 mg/ml. The study confirms the significant anthelmintic activities of bark extract of Artabotrys hexapetalus and therefore demands the isolation of active principles through bioassay.
Fatty Acid Pattern and Alkaloids of Echium RauwolfiiEditor IJCATR
The GC/MS analysis of hexane extract revealed the presence of palmitic acid as saturated fatty acid (1.05%), versus oleic acid (2.18%), linoleic acid (1.13%), cis-8,11,14-eicosatrienoic acid (2.12%) as unsaturated fatty acids. On the other hand, CH2Cl2 extract contained palmitic acid methyl ester (3.55%), and methyl isostearate (1.17%) as saturated fatty acids, versus linoleic acid methyl ester (3.57%) and linolenic acid methyl ester (10.01%) as unsaturated fatty acids. The GC/MS analysis of the alkaloid-rich fraction indicated the presence of the pyrazolidine alkaloids petranine (2.97%), 7-angeloyl-9-(2-methylbutyryl) retronecine (4.22%), 7-angeloylretronecine (0.59%) and 9-angeloylretronecine (0.47%).
The butanol extract showed the heights DPPH radical scavenging activity (IC50 = 14.3 µg),. while ethyl acetate extract was very weak in activity (IC50 = 432.3 µg) and no activity with hexane and methylene chloride extract.
The antimicrobial potentials of E. rauwolfii extracts were examined. The inhibition of the fungi species by ethyl acetate extract exert was comparable to Amphotericin B. The inhibition zone of the butanol extract against Streptococcus pneumonia was comparable to Ampicillin, against Pseudomonas aeruginosa was comparable to Gentamicin and Escherichia coli was comparable to Gentamicin.
The cytotoxicity against HePG-2 of ethyl acetate extract and butanol extract were “very strong”, and that of hexane extract and methylene chloride extract were “moderate”, against MCF-7 of ethyl acetate extract and butanol extract were “strong”, that of methylene chloride extract was “moderate”, and that of hexane extract was “weak” and against HCT-116 of butanol extract was “very strong”, of ethyl acetate extract was“strong”, of methylene chloride extract and hexane extract were “moderate”.
RESIDUAL ESTIMATION OF ISOPROTURON, ATRAZINE AND GRAIN PROTECTANTS IN STORED ...ijabjournal
This study was carried out to see the occurrence and level of pesticide residues in stored wheat grain samples. Wheat grains were collected from different godowns located in Faisalabad district. Weedicide residues in wheat grains were analyzed by using thin plate liquid chromatography and validated high
performance liquid chromatography. On the basis of extent of inhibition of the Hill reaction, it was found that the level of atrazine was (0-0.028 ngkg-1) and the level of isopproturon was (0-0.024 ngkg-1) in test samples. The calculated values were below the Maximum Residue Limit (MEL) i.e. 100 ngkg-1 for atrazine and 2200 ngkg-1 for isoproturon. It was found that presence of herbicide residues in stored wheat was negligible. Further, on wheat grain samples placed in jars and treated with deltamethrin and cypermethrin @0.4 and 0.8% concentration. The persistency data was calculated after 1 hour, 7, 15 and 30 days of treatment. The grain protectants were continuously degraded with the passage of time and after the 30 days
the amount of residues of the grain protectants was found to be very small. It was concluded that the use of the grain protectants in the powder form in combination with other botanicals on stored wheat samples is feasible to reduce the insect pest in stored grains.
Chemical Composition And Acridicid Properties Of The Moroccan Tanacetum Annuu...inventy
Research Inventy : International Journal of Engineering and Science is published by the group of young academic and industrial researchers with 12 Issues per year. It is an online as well as print version open access journal that provides rapid publication (monthly) of articles in all areas of the subject such as: civil, mechanical, chemical, electronic and computer engineering as well as production and information technology. The Journal welcomes the submission of manuscripts that meet the general criteria of significance and scientific excellence. Papers will be published by rapid process within 20 days after acceptance and peer review process takes only 7 days. All articles published in Research Inventy will be peer-reviewed.
COMPARATIVE STUDY OF THE ANTIFUNGAL EFFECT OF OILS AND THEIR UNSAPONIFIABLE F...EDITOR IJCRCPS
The main objective of the study was to assess the in vitro antifungal potency of the unsaponifiable fraction extracted
from coat and bark seeds oils of Citrullus colocynthis L against pathogenic fungal strains namely Aspergillus flavus,
Aspergillus ochraceus, Penicillium expansum and Fusarium oxysporum. In terms of the physico-chemical
characterization, oils under study showed evidence of quality standards relating to vegetable oils. Unsaponifiable
matter yield recorded was approximately 0.93% and 1.03%, for the seed coat oil and bark seed oil respectively.
Antifungal activity carried out by radial growth on solid medium (Potatoes Dextrose Agar acidified) revealed that the
oils and the corresponding unsaponifiable fractions exhibited complete inhibition of fungal growth. Maximal antifungal
index inhibition (IAF=100%) were recorded at 5% and 2.5% dilutions of each fraction tested. The results provided
evidence that the unsaponifiable oils fractions might indeed be potential sources of natural antifungal agents and
deserve further studies to characterize the biological compounds included in these fractions.
Keywords: Citrullus colocynthis seeds, seed coat, bark, oils, unsaponifiable fraction, antifungal activity.
Anti Bacterial and Anti Oxidant Activities of Evolvulus Alsinoides LinnIOSRJPBS
: Plants are very important sources of potential useful raw materials as natural chemotherapeutic agents. Shankhpushpi is botanically termed as Evolvulus alsinoides; the extracts have exhibited antioxidant, anti-ulcer, and immunomodulatory activities. The present work is to investigate the invitro therapeutic potential activities of methanolic extract of whole plant of Evolvulus alsinoides on the antimicrobial activity against five clinical pathogenic bacterial strains viz., Staphylococcus aureus, Bacillus cereus, Escherichia coli, Klebshiella pneumonia and Salmonella typhii, using agar well diffusion assay and Anti oxidant activity using 1-1-Diphenyl- 2-picryl-hydrazyl (DPPH) radical. Extract inhibited IC50: 40.2. Total phenolic content: 28.4 mg/ml & Total flavonoid content: 20.2 μg /ml. Evolvulus alsinoides (MIC): 512.5mg/ml possess 30 to 40 % inhibition, the antibacterial activity of the herbal extracts was more pronounced on the gram- negative bacteria Klebshiella pneumonia than the gram-positive bacteria Staphylococcus aureus. The free radical scavenging activity of methanolic activity of whole plant of Evolvulus alsinoides increase in a concentration dependent manner, and posses statistically significance DPPH free radical scavenging & Anti microbial activities
GC-MS Analysis and Evaluation of In-vitro Antioxidant Potential and Total Phe...Innspub Net
Wild hops (Flemingia strobilifera Linn.) is a shrub belonging to Fabaceae family. The leaves of F. strobilifera are commonly used by the Subanen, the indigenous group in Lapuyan, Zamboanga del Sur, Philippines to treat inflammation. In this study, the hydromethanolic (80%) and aqueous leaf extracts of F. strobilifera were evaluated for their antioxidant activity and total phenolics content. The active semi-volatile components of 80% methanol leaf extract were analyzed using Gas Chromatography-Mass Spectrometry (GC-MS). DPPH radical scavenging activity was used to determine the potential of this plant as anti-oxidant. Total phenolics content was determined using Folin-Ciocalteu reagent and calculated as gallic acid equivalence. GC-MS analysis revealed the presence of eight compounds. Both the 80% methanol and aqueous extracts of F. strobilifera showed significant scavenging activity with low IC50 values of 0.299 mg/mL and <0.25 mg/mL, respectively. There was positive correlation between the scavenging activity percentage and the total phenolics content only in the aqueous extract of F. strobilifera while 80% methanol extract showed negative correlation between inhibition percentage and total phenolics content which can be attributed to the solvent used and method used in quantification of phenolics. Nevertheless, the results suggest that these leaf extracts are potent source of antioxidant compounds and may serve as natural anti-inflammatory agents.
Biochemical and pharmacological study of biologically active preparation of p...inventionjournals
Our aim was to perform some biochemical and pharmacological studies of bioactive bovine placental preparation via digestion of cow placenta using enzyme contained in swine stomach. Amino acid compositions and contents in biologically active preparation of placenta, obtained by digestion of cow placenta with enzyme contained in swine stomach were measured by HPLC technique and it was found that contents of such amino acids as glycine, proline and lysine were highest and 9 essential amino acids, including valine, histidine, methionine, lysine, threonine, arginine, phenylalanine, leucine and isoleucine were measured. In pharmacological study, acute toxicity (LD50) of the preparation and effect of the preparation on immune response to sheep erythrocyte were investigated in white mice, weighing 18 to 20 g each. The study revealed acute toxicity (LD50) of the preparation was 60 ml per kg. Spleen index of the first and second experimental group animals treated by the preparation during both provoked and unprovoked immune responses increased by 1.2 to 3.09 times as compared to that of negative control animals, while splenocyte count elevated by 1.2 to 2.2 times than negative control animals. Higher contents of essential amino acids of the biologically active preparation of cattle placenta shows its biologically higher nutritive value, as well as pharmacological study reveals the preparation has minimal toxicity and higher effect to stimulate immune responses.
protease activity of extracellular enzyme produced by b. subtilis isolated fr...IJEAB
Background: Proteases produced by enzymatic method are more environments friendly than chemical process, and they have tremendous potential in the leather industry and in other several industries. In this study extracellular protease producing non pathogenic Bacillus subtilis was isolated from soil sample and relationship between sporulation and extracellular protease synthesis in large scale cultivation was studied. The enzyme was further characterized, purified, and tested for potential application. Result: The molecular weight of the protease was found to be ~30 KDa. Enzyme activity was checked on the presence of different metal ions and effectors. The enzyme was slightly modulated by MG++ ion, and significantly by Hg++ ion, while Zn++ ion slightly decrease the proteolytic activity. Sulfahydryl reagents, DTT slightly and β-ME significantly inhibit the enzyme. EDTA showed no effect on the enzyme suggesting that the enzyme might not be metalloprotease. PMSF, a known serine protease inhibitor was seen to totally inhibit the enzyme which indicates that the enzyme is a serine protease. The optimum enzyme activity was observed after 22 hours of incubation of B. subtilis at 37o C. Conclusions: Crude enzyme contains 285 units of enzyme which have direct dehairing activity. The enzyme was also seen to be able to remove blood and curry stain from clothes; making it a very promising candidate to be used in a leather and detergent industry. Apart from protease the bacterium was also seen to have lipase and collagenase activity. So, the bacteria are potentially good candidate for industrial application.
Novel Hybrid Molecules of Isoxazole Chalcone Derivatives: Synthesis and Study...Ratnakaram Venkata Nadh
medicine due to their significant role in the treatment of different health problems.
Methods: We have synthesized new series of isoxazole-chalcone conjugates (14a-m) by the
Claisen-Schmidt condensation of suitable substituted acetophenones with isoxazole aldehydes (12a-d).
In vitro cytotoxic activity of the synthesized compounds was studied against four different selected
human cancer cell lines by using sulforhodamine B (SRB) method.
Results: The adopted scheme resulted in good yields of new series of isoxazole-chalcone
conjugates (14a-m). Potent cytotoxic activity was observed for compounds -14a, 14b, 14e, 14i, 14j
and 14k against prostate DU-145 cancer cell line.
Conclusion: The observed potent cytotoxic activities were due to the presence of 3,4,5-
trimethoxyphenyl group.
Synthesis and Pharmacological Evaluation of Novel Heterocyclic CompoundIJSRD
2-[(4-bromo anilino)]-4-chloro-6-morpholino-1,3,5-triazine[2a] and 4-chloro-N-(4-fluorophenyl)-6-morpholino-1,3,5-triazin-2-amine [2b]were synthesized and studied for their biological activity. These compounds were prepared by the condensation of 4-bromoaniline and 4-fluoroaniline with 4-(4,6-dichloro-1,3,5-triazin-2-yl) morpholine [1] which is prepared by the reaction between 2,4,6-trichloro-1,3,5-triazine and morpholine. All the compounds were characterized by elemental analysis and spectral studies.
Synthesis and Characterization of a New Cationic Surfactant Derived from 5-Ch...IJERA Editor
:In this paper, anovel cationic surfactant is synthesized by the alkylation reaction of 5-Chloro- 1H-indole-2,3-dioneunder the conditions of phase transfer catalysis and quaternizationby trimethylamine in acetone solution.Chemical structures of synthesized compounds were confirmed by 1HNMR and 13C NMR. The micellization of cationic surfactant in aqueous solution at room temperature has been reported using the conductivity of the measurements
A STUDY TO EVALUATE THE IN VITRO ANTIMICROBIAL ACTIVITY AND ANTIANDROGENIC E...Dr. Pradeep mitharwal
The present paper deals with synthesis and characterization
of some new chromium (III) Schiff base complexes using microwave irradiation
technique as well as conventional heating. The S∩N donor benzothiazolines, 1-
(2-furanyl) ethanone benzothiazoline (Bzt1N
∩
SH), 1-(2-thienyl) ethanone
benzothiazoline (Bzt2N
∩
SH) and 1-(2-pyridyl) ethanone benzothiazoline
(Bzt3N
∩
SH) were prepared by the condensation of ortho-aminothiophenol with
respective ketones in ethanol.
Degradation of an organophosphorus insecticide (chlorpyrifos) in simulated wa...Salah Hussein
Induced degradation of chlorpyrifos insecticide in simulated wastewater with advanced oxidation processes (AOPs), using ultraviolet irradiation (UV), ozonation and chemical oxidation using (sodium hypochlorite, calcium hypochlorite, monochloride-isocyanuric acid (MCICA), dichloroiso-cyanuric acid (DCICA), trichloroisocyanuric acid (TCICA) ) was studied. Chlorpyrifos and its degradation products were extracted using solid phase extraction (SPE) method, identified using GC-MS. Results showed that the degradation of chlorpyrifos in simulated wastewater followed the first order reaction, and its half life was 3.34, 5.64, 7.13 and 10.69h under ozonation, UV, 1.5%TCICA and 1.5%DCICA respectively when chlorpyrifos solutions treated for 12 h. The concentrations of chemical oxidative substances, active chlorine content and time of treatments had a significant effect on degradation rate of chlorpyrifos, which increased with increasing of each. The most enhancement of chlorpyrifos degradation was observed in treatment with ozonation, UV, TCICA and DCICA where the dissipations % of the parent compounds were 85.70, 57.71, 43.71 and 35.07 %, respectively. The intermediates products of chlorpyrifos degradation using chemical method were identified as O,O-Diethyl thiophosphate(DEP), 3,5,6-trichloro-2-pyridinol(TCP), 3,5,6-trichloro-2-methoxypyridine(TMP) and 2,3,5,6-tetrachloro-pyridine. UV leads to formation of O,O-Diethyl phosphate, TCP and Chlorpyrifos oxon. Ozonation leads to formation of O,O-Diethyl thiophosphate beside the UV degradation products.
Fatty Acid Pattern and Alkaloids of Echium RauwolfiiEditor IJCATR
The GC/MS analysis of hexane extract revealed the presence of palmitic acid as saturated fatty acid (1.05%), versus oleic
acid (2.18%), linoleic acid (1.13%), cis-8,11,14-eicosatrienoic acid (2.12%) as unsaturated fatty acids. On the other hand, CH2Cl2
extract contained palmitic acid methyl ester (3.55%), and methyl isostearate (1.17%) as saturated fatty acids, versus linoleic acid
methyl ester (3.57%) and linolenic acid methyl ester (10.01%) as unsaturated fatty acids. The GC/MS analysis of the alkaloid-rich
fraction indicated the presence of the pyrazolidine alkaloids petranine (2.97%), 7-angeloyl-9-(2-methylbutyryl) retronecine (4.22%), 7-
angeloylretronecine (0.59%) and 9-angeloylretronecine (0.47%).
The butanol extract showed the heights DPPH radical scavenging activity (IC50 = 14.3 μg),. while ethyl acetate extract was very weak
in activity (IC50 = 432.3 μg) and no activity with hexane and methylene chloride extract.
The antimicrobial potentials of E. rauwolfii extracts were examined. The inhibition of the fungi species by ethyl acetate extract exert
was comparable to Amphotericin B. The inhibition zone of the butanol extract against Streptococcus pneumonia was comparable to
Ampicillin, against Pseudomonas aeruginosa was comparable to Gentamicin and Escherichia coli was comparable to Gentamicin.
The cytotoxicity against HePG-2 of ethyl acetate extract and butanol extract were “very strong”, and that of hexane extract and
methylene chloride extract were “moderate”, against MCF-7 of ethyl acetate extract and butanol extract were “strong”, that of
methylene chloride extract was “moderate”, and that of hexane extract was “weak” and against HCT-116 of butanol extract was “very
strong”, of ethyl acetate extract was“strong”, of methylene chloride extract and hexane extract were “moderate”.
Caralluma truncato-coronata (Sedgw.) Gravely &
Mayur is one of the endangered and rare genera of the Dogbane
family, Apocynaceae was extracted by ethanolic Soxhlet method
and was phytochemically screened by GC-MS. Among the
compounds screened, the bioactivity and the name of the
compound viz., Styrene, Deferiprone, Amyl acetate, Cetyl
alcohol, Docosanol, Octadecene, Stearic acid, Phytol,
Diacetylverrucarol, an amine alkene, a terpenoid and a cyano
compound were identified. This report is the first of its kind to
analyse the ethanolic constituents of C.truncato-coronata using
GC-MS.The results of the GC-MS profile can be used as
pharmacognostical tool for the identification of the plant. GC-MS
analysis showed the existence of various compounds with
different chemical structures. The presence of various bioactive
compounds confirms the application of C.truncato-coronata for
various ailments by traditional practioners. The antimicrobial
susceptibility testing on the human pathogenic bacteria and fungi
on Agar Well diffusion Method in MHA significantly recorded
the maximum inhibitory zone in Candida tropicalis and
Enterococcus faecalis.The isolation of individual phytochemical
constituents may proceed to find a novel drug.
Synthesis, Characterization, and Biological Evaluation of Some Novel Phthalim...BRNSS Publication Hub
Objective: Different Phthalimide derivatives (4,5,6,7-tetrachloro-2-[1,2,4]triazol-4-yl]-isoindole-1,3-dione derivatives) were synthesized and biological activities of them were evaluated. Materials and Methods: In the present study, four new phthalimide derivatives were synthesized. The structures of final compounds were characterized on the basis of spectral data. Then, biological evaluation of all the synthesized compounds means in vivo anticancer activity was evaluated on the Ehrlich ascites carcinoma (EAC) bearing Swiss albino mice model, and in vitro antioxidant activity was assessed using 1,1-diphenyl-2-picryl hydrazine (DPPH) radical scavenging assay. Results: The titled compounds (2A-2D) were found to reduce tumor volume, viable cell count and increase non-viable cell count, and percentage increase in life span. All compounds showed significant activity in quenching DPPH free radical. Conclusion: All compounds showed significant (P<0.01) anticancer activity compared to EAC control group. All synthesized compound exhibited considerable antioxidant activity against free radicals, and they showed significant IC50 values and can, thus, ensure protection against oxidative stress.
Facial and Simple Synthesis of Some New (Pyrazole and Triazole) Coumarin Deri...IOSRJAC
2-oxo-2H-coumarin-3-carbohydrazide (2) which prepared from the reaction of ethyl-2-oxo-2Hcoumarin-3-carboxylate (1) with hydrazine hydrate in ethanol containing a catalytic amount of piperidine mixture consider a good and available starting intermediate for synthesis of series of functionalized coumarins. So, compound (2) was utilized as a key for the synthesis of some new (pyrazole, triazole)-2H- coumarin-2-one derivatives by the reaction with some selected reagents.
GC-MS analysis of phytocomponents in Vernonia amygdalina.Del leaves and its c...iosrjce
IOSR Journal of Agriculture and Veterinary Science (IOSR-JAVS) is a double blind peer reviewed International Journal edited by the International Organization of Scientific Research (IOSR). The journal provides a common forum where all aspects of Agricultural and Veterinary Sciences are presented. The journal invites original papers, review articles, technical reports and short communications containing new insight into any aspect Agricultural and Veterinary Sciences that are not published or not being considered for publication elsewhere.
The Role of Cell Wall-Degrading Enzymes in the Development of Anthracnose Dis...Agriculture Journal IJOEAR
— The ability of Colletotrichumtruncatum CP2 in producing pectinolytic and cellulolytic enzymes was evaluated by shake flask fermentations. The results of enzymatic activity experiment indicated that PG was the first cell wall-degrading enzymes detected and the activities obtained were higher (0.24±0.10 U/mL) than other enzymes, which appeared later and in lower amount. After the cell wall was degraded by the action of PG, further degradation of the cell wall was affected by pectin methylesterases, pectin lyase, pectate lyase and cellulases. The disparity in enzymatic activity at different intervals may suggest their specific role for pathogenesis at proper timings.
Comprehensive study on pharmacognostic, physico and phytochemical evaluation ...Uploadworld
Terminalia arjuna Roxb. (Family-Combretaceae) is commonly known as Arjun tree and valued for its medicinal uses. In the present investigation, the detailed pharmacognostic study of T. arjuna stem bark (TASB) is carried out to lay down the standards which could be useful in forthcoming experimental studies.
Similar to The Eco-friendly Marine Gastropod Turbo brunneus (L. 1758) and its Vital role in Future Pharmaceutical Industry Through GC-MS Study (20)
Data Mining is a significant field in today’s data-driven world. Understanding and implementing its concepts can lead to discovery of useful insights. This paper discusses the main concepts of data mining, focusing on two main concepts namely Association Rule Mining and Time Series Analysis
A Review on Real Time Integrated CCTV System Using Face Detection for Vehicle...rahulmonikasharma
We are describes the technique for real time human face detection and counting the number of passengers in vehicle and also gender of the passengers.The Image processing technology is very popular,now at present all are going to use it for various purpose. It can be applied to various applications for detecting and processing the digital images. Face detection is a part of image processing. It is used for finding the face of human in a given area. Face detection is used in many applications such as face recognition, people tracking, or photography. In this paper,The webcam is installed in public vehicle and connected with Raspberry Pi model. We use face detection technique for detecting and counting the number of passengers in public vehicle via webcam with the help of image processing and Raspberry Pi.
Considering Two Sides of One Review Using Stanford NLP Frameworkrahulmonikasharma
Sentiment analysis is a type of natural language processing for tracking the mood of the public about a particular product or a topic and is useful in several ways. Polarity shift is the most classical task which aims at classifying the reviews either positive or negative. But in many cases, in addition to the positive and negative reviews, there still many neutral reviews exist. However, the performance sometimes limited due to the fundamental deficiencies in handling the polarity shift problem. We propose an Improvised Dual Sentiment Analysis (IDSA) model to address this problem for sentiment classification. We first propose a novel data expansion technique by creating sentiment-reversed review for each training and test review. We develop a corpus based method to construct a pseudo-antonym dictionary. It removes DSA’s dependency on an external antonym dictionary for review reversion. We conduct a range of experiments and the results demonstrates the effectiveness of DSA in addressing the polarity shift in sentiment classification. .
A New Detection and Decoding Technique for (2×N_r ) MIMO Communication Systemsrahulmonikasharma
The requirements of fifth generation new radio (5G- NR) access networks are very high capacity and ultra-reliability. In this paper, we proposed a V-BLAST2 × N_r MIMO system that is analyzed, improved, and expected to achieve both very high throughput and ultra- reliability simultaneously.A new detection technique called parallel detection algorithm is proposed. The performance of the proposed algorithm compared with existing linear detection algorithms. It was seen that the proposed technique increases the speed of signal transmission and prevents error propagation which may be present in serial decoding techniques. The new algorithm reduces the bit error probability and increases the capacity simultaneouslywithout using a standard STC technique. However, it was seen that the BER of systems using the proposed algorithm is slightly higher than a similar system using only STC technique. Simulation results show the advantages of using the proposed technique.
Broadcasting Scenario under Different Protocols in MANET: A Surveyrahulmonikasharma
A wireless network enables people to communicate and access applications and information without wires. This provides freedom of movement and the ability to extend applications to different parts of a building, city, or nearly anywhere in the world. Wireless networks allow people to interact with e-mail or browse the Internet from a location that they prefer. Adhoc Networks are self-organizing wireless networks, absent any fixed infrastructure. broadcasting of data through proper channel is essential. Various protocols are designed to avoid the loss of data. In this paper an overview of different broadcast protocols are discussed.
Sybil Attack Analysis and Detection Techniques in MANETrahulmonikasharma
Security is important for many sensor network applications. A particularly harmful attack against sensor and ad hoc networks is known as the Sybil attack [6], where a node Illegitimately claims multiple identities.Mobility cause a main problem when we talk about security in Mobile Ad-hoc networks. It doesn’t depend on fixed architecture, the nodes are continuously moving in a random fashion. In this article we will focus on identifying the Sybil attack in MANET. It uses air medium for communication so it is more prone to the attack. Sybil attack is one in which single node present multiple fake identities to other nodes, which cause destruction.
A Landmark Based Shortest Path Detection by Using A* and Haversine Formularahulmonikasharma
In 1900, less than 20 percent of the world populace lived in cities, in 2007, fair more than 50 percent of the world populace lived in cities. In 2050, it has been anticipated that more than 70 percent of the worldwide population (about 6.4 billion individuals) will be city tenants. There's more weight being set on cities through this increment in population [1]. With approach of keen cities, data and communication technology is progressively transforming the way city regions and city inhabitants organize and work in reaction to urban development. In this paper, we create a nonspecific plot for navigating a route throughout city A asked route is given by utilizing combination of A* Algorithm and Haversine equation. Haversine Equation gives least distance between any two focuses on spherical body by utilizing latitude and longitude. This least distance is at that point given to A* calculation to calculate minimum distance. The method for identifying the shortest path is specify in this paper.
Processing Over Encrypted Query Data In Internet of Things (IoTs) : CryptDBs,...rahulmonikasharma
Internet of Things (IoT) is the developing technologies that would be the biggest agents to modify the current world. Machine-to-machine communications perform with virtual, mobile and instantaneous connections. In IoT system, it consists of data-gathering sensors various other household devices. Intended for protecting IoT system, the end-to-end secure communication is a necessary measure to protect against unauthorized entities (e.g., modification attacks and eavesdropping,) and the data unprotected on the Cloud. The most important concern hereby is how to preserve the insightful information and to provide the privacy of user data. In IoT, the encrypted data computing is based on techniques appear to be promising approaches. In this paper, we discuss about the recent secure database systems, which are capable to execute SQL queries over encrypted data.
Quality Determination and Grading of Tomatoes using Raspberry Pirahulmonikasharma
In India cultivation of tomatoes is carried out by traditional methods and techniques. Today tremendous improvement in field of agriculture technologies and products can be seen. The tomatoes affect the overall production drastically. Image processing technique can be key technique for finding good qualities of tomatoes and grading. This work aimed to study different types of algorithms used for quality grading and sorting of fruit from the acquire image. In previous years several types of techniques are applied to analyses the good quality fruits. A simple system can be implemented using Raspberry pi with computer vision technology and image processing algorithms.
Comparative of Delay Tolerant Network Routings and Scheduling using Max-Weigh...rahulmonikasharma
Network management and Routing is supportively done by performing with the nodes, due to infrastructure-less nature of the network in Ad hoc networks or MANET. The nodes are maintained itself from the functioning of the network, for that reason the MANET security challenges several defects. Routing process and Scheduling is a significant idea to enhance the security in MANET. Other than, scheduling has been recognized to be a key issue for implementing throughput/capacity optimization in Ad hoc networks. Designed underneath conventional (LT) light tailed assumptions, traffic fundamentally faces Heavy-tailed (HT) assumption of the validity of scheduling algorithms. Scheduling policies are utilized for communication networks such as Max-Weight, backpressure and ACO, which are provably throughput optimality and the Pareto frontier of the feasible throughput region under maximal throughput vector. In wireless ad-hoc network, the issue of routing and optimal scheduling performs with time varying channel reliability and multiple traffic streams. Depending upon the security issues within MANETs in this paper presents a comparative analysis of existing scheduling policies based on their performance to progress the delay performance in most scenarios. The security issues of MANETs considered from this paper presents a relative analysis of existing scheduling policies depend on their performance to progress the delay performance in most developments.
DC Conductivity Study of Cadmium Sulfide Nanoparticlesrahulmonikasharma
The dc conductivity of consolidated nanoparticle of CdS has been studied over the temperature range from 303 K to 523 K and the conductivity has been found to be much larger than that of single crystals.
A Survey on Peak to Average Power Ratio Reduction Methods for LTE-OFDMrahulmonikasharma
OFDM (Orthogonal Frequency Division Multiplexing) is generally preferred for high data rate transmission in digital communication. The Long-Term Evolution (LTE) standards for the fourth generation (4G) wireless communication systems. Orthogonal Frequency Division Multiple Access (OFDMA) and Single Carrier Frequency Division Multiple Access (SC-FDMA) are the two multiple access techniques which are generally used in LTE.OFDM system has a major shortcoming of high peak to average power ratio (PAPR) value. This paper explains different PAPR reduction techniques and presents a comparison of the various techniques based on theoretical results. It also presents a survey of the various PAPR reduction techniques and the state of the art in this area.
IOT Based Home Appliance Control System, Location Tracking and Energy Monitoringrahulmonikasharma
Home automation has been a dream of sciences for so many years. It could wind up conceivable in twentieth century simply after power all family units and web administrations were begun being utilized on across the board level. The point of home robotization is to give enhanced accommodation, comfort, vitality effectiveness and security. Vitality checking and protection holds prime significance in this day and age in view of the irregularity between control age and request observing frameworks accessible in the market. Ordinarily, customers are disappointed with the power charge as it doesn't demonstrate the power devoured at the gadget level. This paper shows the outline and execution of a vitality meter utilizing Arduino microcontroller which can be utilized to gauge the power devoured by any individual electrical apparatus. The primary expectation of the proposed vitality meter is to screen the power utilization at the gadget level, transfer it to the server and build up remote control of any apparatus. So we can screen the power utilization remotely and close down gadgets if vital. The car segment is additionally one of the application spaces where vehicle can be made keen by utilizing "IOT". So a vehicle following framework is additionally executed to screen development of vehicles remotely.
Thermal Radiation and Viscous Dissipation Effects on an Oscillatory Heat and ...rahulmonikasharma
An anticipated outcome that is intended chapter is to investigate effects of magnetic field on an oscillatory flow of a viscoelastic fluid with thermal radiation, viscous dissipation with Ohmic heating which bounded by a vertical plane surface, have been studied. Analytical solutions for the quasi – linear hyperbolic partial differential equations are obtained by perturbation technique. Solutions for velocity and temperature distributions are discussed for various values of physical parameters involving in the problem. The effects of cooling and heating of a viscoelastic fluid compared to the Newtonian fluid have been discussed.
Advance Approach towards Key Feature Extraction Using Designed Filters on Dif...rahulmonikasharma
In fast growing database repository system, image as data is one of the important concern despite text or numeric. Still we can’t replace test on any cost but for advancement, information may be managed with images. Therefore image processing is a wide area for the researcher. Many stages of processing of image provide researchers with new ideas to keep information safe with better way. Feature extraction, segmentation, recognition are the key areas of the image processing which helps to enhance the quality of working with images. Paper presents the comparison between image formats like .jpg, .png, .bmp, .gif. This paper is focused on the feature extraction and segmentation stages with background removal process. There are two filters, one is integer filter and second one is floating point Filter, which is used for the key feature extraction from image. These filters applied on the different images of different formats and visually compare the results.
Alamouti-STBC based Channel Estimation Technique over MIMO OFDM Systemrahulmonikasharma
The examination on various looks into on MIMO STBC framework in order to accomplish the higher framework execution is standard that the execution of the remote correspondence frameworks can be improved by usage numerous transmit and get radio wires, that is normally gathered on the grounds that the MIMO procedure, and has been incorporated. The Alamouti STBC might be a promising because of notice the pick up inside the remote interchanges framework misuse MIMO. To broaden the code rate and furthermore the yield of the symmetrical zone time square code for more than 4 transmit reception apparatuses is examined. The outlined framework is beated once forced with M-PSK (i.e upto 32-PSK) regulation. The channel estimation examine in these conditions.
Empirical Mode Decomposition Based Signal Analysis of Gear Fault Diagnosisrahulmonikasharma
A vibration investigation is about the specialty of searching for changes in the vibration example, and after that relating those progressions back to the machines mechanical outline. The level of vibration and the example of the vibration reveal to us something about the interior state of the turning segment. The vibration example can let us know whether the machine is out of adjust or twisted. Al-so blames with the moving components and coupling issues can be distinguished. This paper shows an approach for equip blame investigation utilizing signal handling plans. The information has been taken from college of ohio, joined states. The investigation has done utilizing MATLAB software.
This paper discusses a new algorithm of a univariate method, which is vitally important to develop a short-term load forecasting module for planning and operation of distribution system. It has many applications including purchasing of energy, generation and infrastructure development etc. We have discussed different time series forecasting approaches in this paper. But ARIMA has proved itself as the most appropriate method in forecasting of the load profile for West Bengal using the historical data of the year of 2017. Auto Regressive Integrated Moving Average model gives more accuracy level of load forecast than any other techniques. Mean Absolute Percentage Error (MAPE) has been calculated for the mentioned forecasted model.
Impact of Coupling Coefficient on Coupled Line Couplerrahulmonikasharma
The coupled line coupler is a type of directional coupler which finds practical utility. It is mainly used for sampling the microwave power. In this paper, 3 couplers A,B & C are designed with different values of coupling coefficient 6dB,10dB & 18dB respectively at a frequency of 2.5GHz using ADS tool. The return loss, isolation loss & transmission loss are determined. The design & simulation is done using microstrip line technology.
Design Evaluation and Temperature Rise Test of Flameproof Induction Motorrahulmonikasharma
The ignition of flammable gases, vapours or dust in presence of oxygen contained in the surrounding atmosphere may lead to explosion. Flameproof three phase induction motors are the most common and frequently used in the process industries such as oil refineries, oil rigs, petrochemicals, fertilizers, etc. The design of flameproof motor is such that it allows and sustain explosion within the enclosure caused by ignition of hazardous gases without transmitting it to the external flammable atmosphere. The enclosure is mechanically strong enough to withstand the explosion pressure developed inside it. To prevent an explosion due to hot spot on the surface of the motor, flameproof induction motors are subjected to heat run test to determine the maximum surface temperature and temperature class with respect to the ignition temperature of the surrounding flammable gas atmosphere. This paper highlights the design features of flameproof motors and their surface temperature classification for different sizes.
Welcome to WIPAC Monthly the magazine brought to you by the LinkedIn Group Water Industry Process Automation & Control.
In this month's edition, along with this month's industry news to celebrate the 13 years since the group was created we have articles including
A case study of the used of Advanced Process Control at the Wastewater Treatment works at Lleida in Spain
A look back on an article on smart wastewater networks in order to see how the industry has measured up in the interim around the adoption of Digital Transformation in the Water Industry.
Final project report on grocery store management system..pdfKamal Acharya
In today’s fast-changing business environment, it’s extremely important to be able to respond to client needs in the most effective and timely manner. If your customers wish to see your business online and have instant access to your products or services.
Online Grocery Store is an e-commerce website, which retails various grocery products. This project allows viewing various products available enables registered users to purchase desired products instantly using Paytm, UPI payment processor (Instant Pay) and also can place order by using Cash on Delivery (Pay Later) option. This project provides an easy access to Administrators and Managers to view orders placed using Pay Later and Instant Pay options.
In order to develop an e-commerce website, a number of Technologies must be studied and understood. These include multi-tiered architecture, server and client-side scripting techniques, implementation technologies, programming language (such as PHP, HTML, CSS, JavaScript) and MySQL relational databases. This is a project with the objective to develop a basic website where a consumer is provided with a shopping cart website and also to know about the technologies used to develop such a website.
This document will discuss each of the underlying technologies to create and implement an e- commerce website.
Student information management system project report ii.pdfKamal Acharya
Our project explains about the student management. This project mainly explains the various actions related to student details. This project shows some ease in adding, editing and deleting the student details. It also provides a less time consuming process for viewing, adding, editing and deleting the marks of the students.
Sachpazis:Terzaghi Bearing Capacity Estimation in simple terms with Calculati...Dr.Costas Sachpazis
Terzaghi's soil bearing capacity theory, developed by Karl Terzaghi, is a fundamental principle in geotechnical engineering used to determine the bearing capacity of shallow foundations. This theory provides a method to calculate the ultimate bearing capacity of soil, which is the maximum load per unit area that the soil can support without undergoing shear failure. The Calculation HTML Code included.
Cosmetic shop management system project report.pdfKamal Acharya
Buying new cosmetic products is difficult. It can even be scary for those who have sensitive skin and are prone to skin trouble. The information needed to alleviate this problem is on the back of each product, but it's thought to interpret those ingredient lists unless you have a background in chemistry.
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The automated cosmetic shop management system should deal with the automation of general workflow and administration process of the shop. The main processes of the system focus on customer's request where the system is able to search the most appropriate products and deliver it to the customers. It should help the employees to quickly identify the list of cosmetic product that have reached the minimum quantity and also keep a track of expired date for each cosmetic product. It should help the employees to find the rack number in which the product is placed.It is also Faster and more efficient way.
Hybrid optimization of pumped hydro system and solar- Engr. Abdul-Azeez.pdffxintegritypublishin
Advancements in technology unveil a myriad of electrical and electronic breakthroughs geared towards efficiently harnessing limited resources to meet human energy demands. The optimization of hybrid solar PV panels and pumped hydro energy supply systems plays a pivotal role in utilizing natural resources effectively. This initiative not only benefits humanity but also fosters environmental sustainability. The study investigated the design optimization of these hybrid systems, focusing on understanding solar radiation patterns, identifying geographical influences on solar radiation, formulating a mathematical model for system optimization, and determining the optimal configuration of PV panels and pumped hydro storage. Through a comparative analysis approach and eight weeks of data collection, the study addressed key research questions related to solar radiation patterns and optimal system design. The findings highlighted regions with heightened solar radiation levels, showcasing substantial potential for power generation and emphasizing the system's efficiency. Optimizing system design significantly boosted power generation, promoted renewable energy utilization, and enhanced energy storage capacity. The study underscored the benefits of optimizing hybrid solar PV panels and pumped hydro energy supply systems for sustainable energy usage. Optimizing the design of solar PV panels and pumped hydro energy supply systems as examined across diverse climatic conditions in a developing country, not only enhances power generation but also improves the integration of renewable energy sources and boosts energy storage capacities, particularly beneficial for less economically prosperous regions. Additionally, the study provides valuable insights for advancing energy research in economically viable areas. Recommendations included conducting site-specific assessments, utilizing advanced modeling tools, implementing regular maintenance protocols, and enhancing communication among system components.
The Eco-friendly Marine Gastropod Turbo brunneus (L. 1758) and its Vital role in Future Pharmaceutical Industry Through GC-MS Study
1. International Journal on Recent and Innovation Trends in Computing and Communication ISSN: 2321-8169
Volume: 5 Issue: 11 01 – 09
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The Eco-friendly Marine Gastropod Turbo brunneus (L. 1758) and its Vital role
in Future Pharmaceutical Industry Through GC-MS Study
D. Jayaprabha
Assistant Professor of Zoology, Nazareth Margoschis College at Pillaiyanmanai, Nazareth-628 617,
Tamilnadu, India.
Abstract: Molluscs form valuable fisheries in various parts of the coast of India providing shell fish as food and as source of lime, pearls and
decorative shells, as constituents of medicinal preparations etc. In the present study, the eco-friendly Turbo brunneus and its vital role in future
pharmaceutical industry through GC-MS analysis was carried out. Almost eighteen compounds are obtained through GC-MS which might be
responsible for antimicrobial, pharmaceutical, insect repellent, anti-inflammatory, anticancer, antiasthmatic, diuretic and antiarthritic activities.
Keywords: Molluscs, pharmaceutical industry, GC-MS analysis, antimicrobial, anticancer
__________________________________________________*****_________________________________________________
I. INTRODUCTION
Among the marine organisms, molluscs are one of
the most successful forms of animal life and they have
conquered almost every habitat and exist in all the oceans
(from shallow tidal pools to the deepest trenches). Many
studies on bioactive compounds from molluscs exhibiting
antibacterial, antitumour, antileukemic and antiviral
activities have been reported worldwide (Pettit et al., 1987;
Kamiya et al., 1989; Prem Anand et al., 2002;
Rajaganapathy et al., 2002; Thilaga, 2005; Kathiresan et al.,
2008; Dhinakaran et al., 2011 and Ashok Kumar 2011).
Today most infectious diseases can be brought
under control with natural or synthetic drugs. We are still in
great need of safer, cheaper and effective drugs. Some
marine molluscs have shown pronounced activities, useful
in the biomedical arena. The potential of marine molluscs as
a source of biologically active products is largely
unexplored in India. Hence, a broad based screening of
marine molluscs for bioactive compounds is necessary. A
thorough understanding of chemical structure and biological
activity will lead to the formulation of novel drugs with
specific actions. Considering the above facts the present
study has been undertaken to test the molluscan extracts
against human pathogens and to isolate the bioactive
substance from the most potent extract that purify and
characterize it.
II. MATERIAL AND METHODS
In the present study, the whole body tissues of
Turbo brunneus were used. The freshly collected samples
were cleaned and washed with fresh sea water to remove all
impurities. The shells were removed and the tissues were
then sun dried. From this dried tissues, the crude methanol
extract was prepared.
A. Fractionation
Crude methanol extract was fractioned by silica gel
column chromatography. The extract was fractionated with
five solvent systems. Elutions with Hexane: Chloroform
(1:1), Chloroform (100%), Benzene (100%), Methanol
(100%) and Distilled water in order of their polarity afford
five fractions F1, F2, F3, F4 and F5. A known amount of
extract was taken and their organic solvents were removed
by vaccum evaporation, solids were dissolved in deionised
water and concentration series of 1mg/ml, 10 mg/ml and
100 mg/ml were prepared and the antibacterial activity of
each extract was tested thrice for confirmation.
B. GC-MS
The more potent fraction was characterized to
know the functional groups through GC-MS study at Indian
institute of crop processing Technology-Thanjavur.
GC – MS Analysis
GC-MS analysis was carried out on a GC Clarus
500 Perkin Elmer system comprising a AOC 20i auto
sampler and gas chromatography interfaced to a mass
spectrophotometer (GC-MS) instrument employing the
following conditions such as Column Elite – 5 MS fused
silica capillary column (30 x 0.25 mm ID x 0.25 µm df,
composed of 5% Diphenyl/95% Dimethyl poly siloxane),
operating in electron impact mode at 70eV: Helium
(99.999%) was used as a carrier gas at constant flow of
1ml/min and an injection volume of 3 µl (split ratio of 10:1)
injector temperature 2500
C. The oven temperature was
programmed from 1100
C (isothermal for 2 min), with an
increase of 100
C/min to 2000
C, then 50
C/min to 2800
C.
Mass spectra were taken at 70eV; a scan interval of 0.5s and
fragments from 45 to 450 Da.
2. International Journal on Recent and Innovation Trends in Computing and Communication ISSN: 2321-8169
Volume: 5 Issue: 11 01 – 09
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C. Identification of compounds
Interpretation on mass spectrum was conducted
using the data base of National Institute Standard and
Technology (NIST 08s), WILEY 8 and FAME. The
unknown components found in the methanol fraction of the
internal bone were matched with the spectrum of the known
components stored in NIST, WILEY and FAME, the MS
library and predicted from Dukes ethno botanical database.
III. RESULTS
Antibacterial activity was tested against eight
bacterial pathogens with respect to five different fractions of
the crude methanol extracts of body tissues of Turbo
brunneus. As fraction 4 exhibited more potent antibacterial
activity than other fractions, it was further purified with
TLC and characterized through GC –MS.
GC-MS
GC – MS study of fraction 4 from Turbo brunneus
revealed the presence of the following 18 compounds:
Aziridine, 1-methyl, 5-Methyl-2-hexanone oxime,
Cyclohexanol,2-amino-,trans, Azocine, octahydro, Butanal,
O-methyloxime, L-Homocitrulline, Pentanal, oxime, E-2-
Tetradecen-1-ol, Z-10-Pentadecen-1-ol, Lysine,
Trimethylamine, compd. with borane (11), 1,1-
Cyclopropanedicarbonitrile, 2,2-dimethyl, Cyclopentanol, 2-
(aminomethyl)-, cis, Tricyclo[4.2.1.1(2,5)]decan-3-ol, n-
Nonanoylmorpholine, Thiomorpholine, Semioxamazide and
Cholan-24-oic acid, 3-oxo-, methyl ester, (5á). Of which
fifteen antimicrobial compounds were conferred by GC-MS
analysis, when F4 fraction (Methanol 100%) of Turbo
brunneus extract was injected viz: Aziridine, 1-methyl,
Cyclohexanol,2-amino-,trans, Azocine, octahydro, Butanal,
O-methyloxime, L-Homocitrulline, Pentanal, oxime, E-2-
Tetradecen-1-ol, Z-10-Pentadecen-1-ol, Trimethylamine,
compd. with borane (11), 1,1-Cyclopropanedicarbonitrile,
2,2-dimethyl, Cyclopentanol, 2-(aminomethyl)-, cis,
Tricyclo[4.2.1.1(2,5)] decan-3-ol, Thiomorpholine,
Semioxamazide and Cholan-24-oic acid, 3-oxo-, methyl
ester, (5á) (Table 21). A steroid compound Cholan-24-oic
acid, 3-oxo-, methyl ester, (5a)-with maximum percentage
(50.06%) and a Ketone compound 5-methyl-2-hexanone
oxime with minimum percentage (0.33%) were identified as
antimicrobial compounds (Fig 1– 19 and Table 1).
IV. DISCUSSION
In the present study, a pronounced antibacterial
activity has been observed against some bacterial strains.
The F1, F2, F3, F4 and F5 and crude extracts of Turbo
brunneus showed activity against all bacterial strains tested.
4 of Turbo brunnues was found to be more potent
and it was purified and characterized through GC-MS study.
F4 revealed the presence of 18 active compound viz:
Aziridine, 1-methyl, 5-Methyl-2-hexanone oxime,
Cyclohexanol,2-amino-,trans, Azocine, octahydro, Butanal,
O-methyloxime, L-Homocitrulline, Pentanal, oxime, E-2-
Tetradecen-1-ol, Z-10-Pentadecen-1-ol, Lysine,
Trimethylamine, compd. with borane (11), 1,1-
Cyclopropanedicarbonitrile, 2,2-dimethyl, Cyclopentanol, 2-
(aminomethyl)-, cis, Tricyclo[4.2.1.1(2,5)]decan-3-ol, n-
Nonanoylmorpholine, Thiomorpholine, Semioxamazide and
Cholan-24-oic acid, 3-oxo-, methyl ester, (5á).
The present study based on various analysis
revealed the presence of alkaloid, Ketone, amino, nitrogen,
aldehyde, alcoholic, sulphur and steroid compounds (Table
1). These identified probable antimicrobial compounds
which might be responsible for the inhibition of
antimicrobial activity in various fractions during the study.
These above identified compounds were already been
established as an antimicrobial agent by so many authors
(De Vries and Beast, 1995; De Lucca, 2000; Hancock, 2000;
Welling et al., 2000 and Selitrennikoff, 2001).
The GC-MS study of Turbo brunneus reveals the
probable antimicrobial compounds such as Aziridine, 1-
methyl, Cyclohexanol,2-amino-,trans, Azocine, octahydro,
Butanal, O-methyloxime, L-Homocitrulline, Pentanal,
oxime, E-2-Tetradecen-1-ol, Z-10-Pentadecen-1-ol,
Trimethylamine, compd. with borane (11), 1,1-
Cyclopropanedicarbonitrile, 2,2-dimethyl, Cyclopentanol, 2-
(aminomethyl)-, cis, Tricyclo[4.2.1.1(2,5)]decan-3-ol,
Thiomorpholine, Semioxamazide and Cholan-24-oic acid, 3-
oxo-, methyl ester, (5á) which are responsible for the
inhibition of A. hydrophilla, E. coli and S.typhi. The present
findings are in agreement with Emiliano Manzo et al.,
(2007), who reported that two novel triterpenoids, aplyosils
A and B BEtzionin a tyrosin derived compound exhibited
antibacterial activity against Bacillus subtilis. (Lindquist and
Fenical, 1990). High concentrations of macrolides, extracted
from the egg of Spanish dancer nuetibranch, Hexabranchus
sanguineous prevented the growth of pathogenic micro
organism (Pawlik, 1992).
The GC-MS spectra from Turbo brunneus extract
provided a complete carbon skeleton of Aziridine, 1-methyl,
5-Methyl-2-hexanone oxime, Cyclohexanol,2-amino-,trans,
Azocine, octahydro, Butanal, O-methyloxime, L-
Homocitrulline, Pentanal, oxime, E-2-Tetradecen-1-ol, Z-
10-Pentadecen-1-ol, Lysine, Trimethylamine, compd. with
borane (11), 1,1-Cyclopropanedicarbonitrile, 2,2-dimethyl,
Cyclopentanol, 2-(aminomethyl)-, cis,
Tricyclo[4.2.1.1(2,5)]decan-3-ol, n-Nonanoylmorpholine,
Thiomorpholine, Semioxamazide and Cholan-24-oic acid, 3-
3. International Journal on Recent and Innovation Trends in Computing and Communication ISSN: 2321-8169
Volume: 5 Issue: 11 01 – 09
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oxo-, methyl ester, (5á) which might be responsible for the
inhibition of bacterial growth in the present study, similar
finding was reported by way on Mudianta et al., (2010) in
an Indonesian sponge Helichondria sps. has provided 3-akyl
piperidine alkaloids tetradehydrohaliclona-cyclamine A, the
mono-N-oxide and a C-2 epimer. Brominated indoles 6-
bromo 2-methylthio indolin -3-one extracted from
Australian muricid Dicathdis orbita has been identified as
anticancer drug indole derivatives of 6,6’ dibromoindigo
have been antimicrobial activity (Benkendorff et al., 2001).
An alkaloid Batzelladine L and M isolated by Hua et al.,
(2007) inhibits S. aureus.
In the present study, the potent antibacterial
compounds could be detected from Turbo brunneus. Since,
Turbo brunneus is surviving in intertidal rocky shore with
coral reef area, either to protect from their enemies or
through food the animal might synthesis the chemicals
which might be responsible for the inhibition of bacterial
growth. These antibacterial compounds can be relatively
synthesized, chemically modified, analyzed and
manipulated. However, these compounds are also primarily
translational products of genes with potent biological
activity and can be manipulated by techniques of modern
molecular genetics confers the antibacterial compounds
from Turbo brunneus an important role in expanding bridge
between bioactive drug and molecular genetics.
V. REFERENCES
[1] Ashok kumar. P. 2011. Antimicrobial compounds with
therapeutic potential from Certhidea cingulate against
human and fish pathogens. Romanian Biotechnological
letters. Vol. 16 (4). 6401-6406.
[2] Benkendorff, K., A.R.davis, and J.Bremner, 2001.
Chemical defence in the egg masses of benthic
invertebrates. An assessment of antibacterial activity in
39 molluscs and 4 polychactes. J. Invertbr. Pathol.,
78:109-118.
[3] De Lucca, AJ, 2000. Antifungal peptides potential
candidates for the treatment of fungal infections. Expert
opinion on Investigatiional drugs, 9(2) : 273-299.
[4] De Vries, D.J and Beart, P.M., 19995. Fishing for drugs
from sea status and strategies tips, 16 : 275-279.
[5] Dhinakaran A, Sekar. V.Sethubathi, G.V. Suriya. J.
2011. Antipathogenic activity of marine gastropoda
(Hemifusus pugilinus) from Pazhayan, SouthEast Coast
of India . International Journal of Enotl. Sciences. 2(2):
524-530.
[6] Emiliano Manzo, Margherita Gavagnin, Giuseppe
Bifulco, Paola Cimino, Simone Di Micco, M. Letizia
Ciavatta Yue Wei Guo and Guido Cinino 2007.
Aplysiols A and B, Squalene derived polythers from
mantle of the sea hare Aplysia dactylomela. Tetrahedron,
63: 9970-9978.
[7] Hancock, R.E.W., 2000. Cationic antimicrobial peptides
towards clinical applications. Expert opinion on
Investigational drugs. 9 : 1723-1729.
[8] Hua. J., Yamarthy. R and S. Felsenstein 2007. Activity
of antimicrobial peptide mimetics in the oral cavity:1.
Activity against biofilms of Candida albicans 25(6):
418-425.
[9] Kamiya, H., K. Muromoto, G.K.Sakai, Y.M. Endo and
M. Yamamzaki, 1989. Purification and Characterization
of an antibacterial and antiplastic protein secretion of a
sea hare Aplysia Juliana. Toxicon., 27(12):1262-1277.
[10] Kathiresan. K. M. A. Nabeel and S.Manivannan.2008.
Bioprospecting of marine organisms for novel bioactive
compounds scientific transactions in Environment and
Technovation 1(3):107- 120.
[11] Lindquist N, Fenical W, 1990. Polycarpamines A.E,
antifungaldisulfides from the marine ascidian Polycarpa
auzata. Tetrahedron letters, 31:2389-2392.
[12] Pawlik, J.P., 1992. The Spanish dancer Nudibranch.
Oceanus, Spring: 85-86.
[13] Pettit, G.R., Y.Kamano, C.L. Herald, A.A.Tumman, E.E.
Boettner, H.Kizu, J.M.Schimidt, L.Baczynsky,
K.B.Tomer and R.J.Bontems, 1987. The isolation and
structure of remarkable marine animal antieoplastic
constituent dolastatin-14. J.org. Chem.., 55: 2989-2990.
[14] Prem Anand. T. , and J.K. Patterson Edward, 2002.
Antibacterial activity in the tissue extracts of five
species of Cowries cypraea sps. (Mollusca:
Gastropoda)and ascidian Didemnum psammathodes
(Tunicata:Didemnidae) Indian. J. Mar. Sci., 31:239-242.
[15] Rajaganapathi, J., and K.Kathiresan, 2002. Heparinase in
purple fluid of the sea hare, Bursatella leachii, Curr. Sci,
82:264-266.
[16] Seliternnikoff. C.P.2001. Antifungal proteins. Applied
Environ. Microbiol., 67: 2883-2894. PMID:11425698.
[17] Thilaga, R.D., 2005. Studies on some ecological aspects
of Babylonia spirata (Linn) along the Tuticorin coast.
Ph.D., Thesis, Manonmaniam Sundaranar University,
India.
[18] Wayon Midianta, Peter, L. Katavic, K. Lyvette and
Lambertt., 2010. Structure and absolute configuration of
Z-alkylpiperidine alkaloids from an Indonesian sponge of
the genus Halichondria. Tetrahedron, 66:2752-2760.
[19] Welling, M.M., A.Palusma- Annema, H.S. Batter,
E.K.Pauwels and P.H.Nibbering, 2000. Tenehtium-99 m
labelled antimicrobial peptide discriminate between
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4. International Journal on Recent and Innovation Trends in Computing and Communication ISSN: 2321-8169
Volume: 5 Issue: 11 01 – 09
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Table 1 Activity of Components identified in the methanol column fraction (F4) of extract of Turbo brunneus [GC MS
study]
No. RT Name of the compound
Molecular
formula
Peak
Area %
Compound
Nature
**Activity
1. 3.15 Aziridine, 1-methyl- C3H7N 4.75 Alkaloid Antimicrobial Antiinflammatory
2. 10.37 5-Methyl-2-hexanone oxime C7H15NO 0.33
Ketone
compound
No activity reported
3. 10.85 Cyclohexanol,2-amino-,trans- C6H13NO 1.73
Amino
compound
Antimicrobial
4. 11.41 Azocine, octahydro- C7H15N 0.85
Nitrogen
compound
Antimicrobial
5. 12.59 Butanal, O-methyloxime C5H11NO 7.29
Aldehyde
compound
Antimicrobial
6. 13.20 L-Homocitrulline C7H15N3O3 22.39
Nitrogen
compound
Antimicrobial
7. 14.35 Pentanal, oxime C5H11NO 0.88
Aldehyde
compound
Antimicrobial
8. 14.76 E-2-Tetradecen-1-ol C14H28O 1.77
Alcoholic
compound
Antimicrobial
9. 15.47 Z-10-Pentadecen-1-ol C15H30O 1.62
Alcoholic
compound
Antimicrobial
10. 15.71 Lysine C6H14N2O2 1.73 Amino acid Nutrient
11. 15.97
Trimethylamine, compd. with
borane (11)
C3H12BN 0.59
Amino
compound
Antimicrobial
12. 16.89
1,1-Cyclopropane
dicarbonitrile, 2,2-dimethyl-
C7H8N2 1.18
Nitrogen
compound
Antimicrobial
13. 17.46
Cyclopentanol, 2-
(aminomethyl)-, cis-
C6H13NO 0.41
Amino
compound
Antimicrobial
14. 19.80
Tricyclo[4.2.1.1(2,5)]decan-3-
ol
C10H16O 0.77
Alcoholic
compound
Antimicrobial
15. 20.44 n-Nonanoylmorpholine C13H25NO2 0.63
Nitrogen
compound
Insect repellent
16. 21.01 Thiomorpholine C4H9NS 0.88
Sulphur
compound
Antimicrobial used in Pharmaceuticals
17. 21.31 Semioxamazide C2H5N3O2 2.14
Amino
compound
Antimicrobial
18. 28.85
Cholan-24-oic acid, 3-oxo,
methyl ester, (5á)-
C25H40O3 50.06
Steroid Antimicrobial Antiinflammatory Anticancer
Antiasthma Diuretic Antiarthritic