The document serves as a comprehensive guide to metabolomics, detailing its methods, applications, and significance in biological research. It discusses both untargeted and targeted metabolomics, highlighting advancements that allow for greater detection and analysis of metabolites. Key applications span areas such as clinical diagnostics, pharmacometabolomics, environmental studies, food safety, and plant research, emphasizing the importance of metabolomics in understanding biological systems.

2. The Complete Guide for
Metabolomics Methods
and Application
Edit by:
Creative Proteomics
Shirley, NY,USA

3. Table of Content
1.Brief Introduction for Metabolomics
2.Untargeted metabolomics
3.Targeted metabolomics
4.Features for New Target Metobolomics
5.The application of New Target Metobolomics
6. Conclusion

4. Chapter 1 Brief Introduction
Metabolomics (Metabolomics) is a new system of biological technology
developed by the post-gene era, aimed at the determination of all small
organisms within the metabolites. Compared to Genomics,
Transcriptomics and Proteomics, metabolomics directly and accurately
reflects the current state of the organism and tell us what happens to
the organism instead of predicting what may happen! Metabolomics
includes untargeted metabolomics, targeted Metabolomics and next-
generation target metabolomics according to their detection of
metabolites.

5. Chapter 2 Untaget
Metabolomics
It will scan all the small molecule ions (50Da ~ 1200Da) in the sample,
and obtain the ion mass spectrum. The metabolites are identified by
comparison with the database. The most commonly used analytical
platform for untargeted metabolomics is GC-MS. However, GC-MS still
is not perfect for metabolomics applications. Such as, GC-MS uses single
mass spectrometry and lack of fragment ion information which will lead
to many compounds can not be accurately Identification. Besides, there
are other platforms can be applied for metabolites analysis: NMR
platform and LC-Q-TOF. But untarget metabolomics can only provide
the relative abundance of metabolites, not the absolute concentration
of metabolites.

6. Chapter 3 Targeted
metabolomics
The traditional targeted metabolomics can only detect about 10 metabolites
with the limitation of instrument conditions. But it has been raid developed
with the improvement of instrument technology and become new bright spot
for metobolomics. New targeted metabolomics can simultaneously quantify
more than 1000 metabolites and reduce the time of post-processing data
which can significantly improve the efficiency of metabolomics, making the
biomarker screening process become more simple and reliable with less
metabolic product verification steps.

7. Chapter 4 Features for New
Target Metobolomics
1.More metabolites detection - Different from traditional LC-MS or LC-UV
technology, with the new positive and negative conversion technology
(Polarity Switch), new target metabolomics can scan up to 1000 metabolites in
a period, greatly improving the efficiency of metabolic products at the same
time detection.
2.Easy to identify metabolites - A metabolite will be identified by its parent
ion, daughter ion, and Collision Energy in Multiple Reaction Monitoring (MRM)
technology. Two-step screening before getting into the detecor highly improve
the accurancy of methobolites identification.

8. 3.Easy to handle follow-up ioinformatics - New target metobolimics can
analyze metabolites without the time-consuming database search and
compound identification. And analysis results can be used for multiple
comparative analysis, principal component analysis, metabolic pathway
analysis and other biological large data processing directly.

9. Chapter 5 The application of
New Target Metobolomics
It shows out great potential in various fields:
A. Clinical Metabolomics: Compared with the normal omics, it can reveal the molecular
mechanism of the disease with early diagnosis of blood and urine markers, intestinal and
oral metabolism.
B. Pharmacometabolomics (Pharmacometabolomics): to reveal the role of drug targets
(efficacy studies, including traditional Chinese medicine), rapid and comprehensive
assessment of the toxicity of drugs to assess the interaction between drugs and patients
(personalized pharmaceutical) and so on.

10. C. Environmental Metabolomics: to reveal the impact of the
environment on organisms, high concentrations of environmental
pollutants, pollutant decomposition, migration, to explore the impact of
pollutants on marine ecology.
D. Food Metabolomics: Food Nutritional Function Assessment; Food
Safety Assessment; Antibiotics, Antibiotics, Pesticides and Insecticide
Testing in Food.

11. E. Plant Metabolomics: The use of a new generation of targeted
metabolomics can reveal the mechanism of plant disease and insect
resistance, the mechanism of plant stress resistance (drought, salt,
heavy metals, etc.), plant gene function, plant-microbial interaction
Research, plant primary and secondary metabolic pathways.
F. Transplants Metabolomics: Use metabolomics to monitor the effect
of surgery and transplantation.

12. Chapter 6 Conclusion
As a newly developed discipline and an important part of systemic of biology
following genomics and proteomics, metobolomics can determine the
pathophysiological state of the organism, and it is possible to identify the
associated biomarkers to provide a predictive platform for the associated
warning signal. Compared with genomics and proteomics, metabolomics
research focuses on the specificity of the relevant specific components, and
ultimately involves the study of the commonality, characteristics and regularity
of each metabolic component. Although metobolomics service is still in its
start stage and the analysis methods also need to improve, we believe that
metabonomics research will become a powerful means for efficient and
accurate human disease diagnosis with the continuous improvement and
optimization of its methods.