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This study aimed to discover tissue-specific utilization of glutathione (GSH) during zebrafish embryonic development to identify sensitive targets of oxidative stress. The researchers measured GSH concentrations in different tissues at 6 developmental stages using monochlorobimane dye, which fluoresces upon reacting with GSH. They found the yolk sac maintained high GSH levels throughout development, while the eye, heart and cerebellum displayed transient peaks, indicating tissue-specific GSH utilization changes over time. Understanding spatial and temporal GSH dynamics may help predict how chemical exposures could affect different tissues during development.
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This summary provides the key points from the document in 3 sentences:
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Advanced Ambrotose Study
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Este documento presenta la caracterización de cinco docentes y su institución educativa en Sogamoso, Boyacá. Se describe brevemente el diagnóstico inicial de los estudiantes en inglés y el contexto sociocultural. Finalmente, se incluye una guía para el diseño de una secuencia didáctica sobre el uso de las TIC para fortalecer los conocimientos sobre el universo.
Cathy Southard Resume
Cathy Southard is seeking a position in healthcare where she can utilize her education and experience. She has a diploma in medical assisting and administration from Gwinnett College. Her skills include medical terminology, coding, billing and various clinical skills. She has over 10 years of experience in medical offices, facilities management, and accounting roles. Her most recent role was as a medical assistant where she performed clinical duties and administrative tasks for an internal medicine practice.
James Bowman's Resume' (personal)
This document provides a summary of James L. Bowman III's career experience and qualifications. It includes his education background with degrees in computer science and mathematics. It also lists his current security clearances and over 25 years of experience as a senior software engineer and team lead for various government contractors, where he has specialized in developing simulation, data acquisition, and human-machine interface applications for the Department of Defense. His roles have involved requirements gathering, software design, implementation, testing, and customer support.
Investigación Uso de Estrategias educativas
El documento describe la importancia de implementar estrategias pedagógicas en los estudiantes para mejorar su rendimiento académico. Explica que el desarrollo del niño depende del apoyo familiar y las estrategias usadas por los maestros. Al implementar diferentes estrategias como el juego y la atención personalizada, se encontró que los estudiantes pueden identificar mejor sus fortalezas y debilidades, y ser personas más integrales.
ION Bangladesh - IETF Update
11 April 2016 - ION Bangladesh - What’s happening at the Internet Engineering Task Force (IETF)? What RFCs and Internet-Drafts are in progress related to IPv6, DNSSEC, Routing Security/Resiliency, and other key topics? We’ll give an overview of the ongoing discussions in several working groups and discuss the outcomes of recent Birds-of-a-Feather (BoF) sessions, and provide a preview of what to expect in future discussions.
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Інтерактивні технології у методичній роботі як засіб підвищення професійного ...
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Form 6A-Application for inclusion of name in electoral roll by an overseas el...
Form 6A-Application for inclusion of name in electoral roll by an overseas el...
Rue de la Banque - Avril 2016 - Contribution des technologies de l’informatio...
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Mol. Cell. Biol.-2016-Singh-1990-2010
This document summarizes a study examining the role of the Brg1 gene during early embryonic development in mice. The researchers conditionally deleted the Brg1 gene beginning at gastrulation using a tamoxifen-inducible Cre system. They found that Brg1 deficiency resulted in increased apoptosis, growth retardation, and embryonic death. Gene expression analysis revealed upregulation of genes that negatively regulate cell growth and cell cycle progression. Additionally, the p53 protein accumulated in Brg1-deficient embryos and activated p53-dependent pathways. The researchers provide evidence that Brg1 functions in part via regulating the p53 program to constrain gene expression and facilitate rapid embryonic growth.
Survivin Immunoreactivity in the Gastric Mucosa of Rats Feedind with Carpet S...
Survivin has been studied many times because of its overexpression in several types of cancer
including lung, kidney, skin, endometrium, stomach, colon, breast, prostate, over, hematologic, head and neck
cancers, histopathology features and polymorphisms in the promoter region which belongs to the inhibitör of
apoptosis gene family by researchers. There is no study of survivin immunoreactivity in the gastric mucosa of the
rats fed with carpet shell clam grown in the Dardanelles. In this study, it was aimed to investigate the effects of
carpet shell clam fed rats on survivin production in the gastric mucosa. The carpet shell clam given as food to the
rats were removed from the Dardanelles Çardak region. Four groups of rats are included in the study, group 1
(n=6), control group fed with standard rat food, group 2 (n=6), 75% carpet shell clam and 25% standard rat
food daily, group 3 (n=6), 75% carpet shell clam and 25% standard rat food every two days, group 4 (n=6), 75%
carpet shell clam and 25% standard rat food every three days. To detect survivin localization in the tissues, the
LAB-SA Detection System was used. Survivin immunoreactivity was detected of epithelial cells in the gastric
mucosa of rats fed with carpet shell clam. After the immunohistochemical staining processing all gastric tissue
samples are evaluated in terms of survivin immunoreactivity with light microscopy and image analysis software.
Survivin immunoreactivity was detected 0% in the first group, 83.33% in the second group, 61.83% in the third
group and 32.67% in the fourth group. There was statistically significant difference between the survivin
immunoreactivity in the gastric gland cells of the rats in the experimental and control groups (p> 0.05). Survivin
production in the gastric mucosa of rats suggests that consumption of carpet shell clam may cause tissue damage.
Effect of Heavy Metals on the Activity Levels of Hepatic Enzymes in the Mater...
Effect of Heavy Metals on the Activity Levels of Hepatic Enzymes in the Mater...SSR Institute of International Journal of Life Sciences
ABSTRACT- An experimental study was performed with viviparous animal Heterometrous fulvipes to access the cumulative effect of chronic heavy metals exposure on the activity levels of the enzymes aspartate aminotransferase (AST) and alanine aminotransferase (ALT). Chronic heavy metal exposure resulted in variation in the enzymes levels with increase in AST and decreases in ALT, contributed to the stress induced by the heavy metals. These changes in enzymatic activity of the maternal and embryonic tissue of H. fulvipes under the influence of heavy metal, mercury and lead is suggestive of the specific impact of mercury and lead on the enzymatic pathway, prompting a further study to consolidate the finding in human study. It is pertinent that the heavy metal toxicity be well documented and appropriate precaution taken in mother and fetus to decrease its detrimental effects. Key-words- Heavy Metals, Animal models, Hepatic Enzymes, ViviparousIntroductiondata
This document summarizes a study that examined the effects of culture environment on the mechanical and biochemical properties of cartilage tissue engineered using chondrocytes cultured in agarose gel constructs. The study found that total GAG synthesis was higher in constructs with 4% agarose gel concentration after 7 days of culture. Mechanical testing showed the peak load stress was lower in constructs as the agarose concentration increased. Analysis of the constructs indicated that the total GAG synthesis and mechanical strength of the engineered tissue can be influenced by the agarose concentration and culture time.
Radical Life Extension_Dr. Leon Peshkin_Dec 2023
Presentation for mHealth Israel by Dr. Leon Peshkin, Harvard Medical School, exploring research into Embryology and Longevity, emphasizing Germline and protein aggregates. Includes current overview, anti aging through history, Lifespan Expansion, ALEC: Animal Life Expectancy in Controls, Scalable Platform in Pharmacobiology, Embryos Cleanse Protein Aggregates, Lysosomal switch triggers proteostasis renewal, Xenopus, Rejuvenation Roadmap, Citizen Science Approach to Aging, Xenopus: Maternal Dowry Molecules, Human Disease Modeling
Beneficial Effects of Curcumin Inmaternal and Fetal Oxidativestress and Brain...
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This study investigated the protective effects of curcumin against lead-induced oxidative stress and brain damage in pregnant rats and their fetuses. Pregnant rats were exposed to two doses of lead acetate, with or without curcumin supplementation. Lead exposure resulted in toxicity for both mothers and fetuses, as well as increased oxidative stress and brain histopathology. Co-treatment with curcumin significantly reduced lead levels and oxidative stress, and improved brain histopathology. The results suggest that curcumin has an ameliorative effect against lead-induced neurotoxicity by reducing oxidative stress and reversing tissue damage.Hyperlipidemia & Diabetes & Gut Microbiota
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Thymoquinone ameliorates oxidative damage and histopathological changes of de...
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ABSTRACT
Lead (Pb) toxicity is known to be a chief environmental health issue, especially for pregnant
women and young children. Today, the use of medicinal herbs in the treatment of many diseases
and different toxic agents has become highly accepted due to their effectiveness and lower costs.
Thymoquinone (TQ), which is extracted from Nigella sativa seeds, is a potent antioxidant and anti
inflammatory agent. This study was designed to explore the optional protectivity of TQ against
maternal and fetal oxidative stress and brain damage induced by Pb administration. Pregnant
rats were distributed into seven groups: control group, TQ group, DMSO group, two groups Pb
treated (160 and 320 ppm), and two groups Pb-treated (160 and 320 ppm) co-treated with TQ.
Administration started from gestation day 1 (GD1) to day 20 (GD20) through oral gavage once
daily. Lead administration caused a dose-dependent toxicity for both mothers and fetuses. Also,
the histopathological assessment of the brains from Pb-treated groups showed marked altera
tions. Co-treatment of with TQ and Pb caused a significant decrease in Pb levels as compared
with those treated with Pb alone and amelioration of histopathological changes in the brains. It
was concluded that co-treatment of TQ along with gestational Pb exposure could mitigate the
effects against Pb-induced maternal and fetal neurotoxicity.
KEYWORDS
Lead; oxidative stress; brain;
Thymoquinone; fetal toxicityDevelopmental Anomalies and Oxidative Stress Responses in Zebrafish (Danio Re...
The discharge of human pharmaceuticals via wastewater treatment plants represents a major threat to non-target aquatic organisms since they are continually exposed throughout their lifespan. The individual effects of the anaesthetic, lidocaine; the cytostatics, ifosfamide and cyclophosphamide; and the antimicrobials, sulfamethoxazole, amoxicillin and erythromycin on 24 hpf (hours post fertilization) zebrafish (Danio rerio) following a 96-h exposure was investigated by evaluating embryonic development, catalase (CAT) enzyme activity, and the gene expressions of CAT, cytosolic superoxide dismutase (SOD1), and mitochondrial superoxide dismutase (SOD2). Lidocaine, cyclophosphamide and sulfamethoxazole induced neurotoxicity (scoliosis, tail malformation) and cardiotoxicity (pericardial edema, bradycardia) in the zebrafish which correlate with their adverse effects in mammals. These observations were linked to oxidative stress as indicated by the significant alteration of CAT activity and amounts of transcripts of SOD1, SOD2, and CAT. The CAT activity and gene expressions of the antioxidants were significantly upregulated at sublethal and levels of ifosfamide, lidocaine, cyclophosphamide, and erythromycin non-toxic to embryonic development in this study which suggest that these antioxidants may play a role in the prevention of teratogenicity in the larvae. The multi-level effect approach adopted in this study provides a better understanding of the mechanisms of toxicity employed by lidocaine, cyclophosphamide and sulfamethoxazole towards zebrafish. This study shows the importance of evaluating stress biomarkers and emphasizes the need for chronic studies in assessing pharmaceutical effects on aquatic organisms.
seminario biologia molecular
This study analyzed glycogen storage and localization of glycogen metabolizing enzymes in the murine uterus from proestrus until implantation. Immunohistochemistry and western blot techniques were used to identify and locate key glycogen synthesizing enzymes in the glandular epithelium, luminal epithelium, and stroma. The results showed glycogen deposits and enzyme expression patterns in the different uterine tissues across the estrous cycle and implantation. The discussion agrees with previous studies that glucose levels must be carefully regulated for embryo development, and that glycogen may act as an energy reserve for preimplanted embryos.
LACTOWHEY powder: Provides body’s defense against cancer
Lactowhey powder is a whey protein supplement that provides several potential health benefits. It contains amino acids that help stimulate immunity and increase glutathione levels, which enhances the body's defense against cancer, free radicals, and carcinogens. Pre-clinical and clinical studies have shown that Lactowhey powder may help prevent muscle loss and weight loss in cancer patients by improving nutritional status and appetite. The whey proteins and other components in Lactowhey powder are easily absorbed and can promote muscle strength and immune function.
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This document discusses a patented milk serum (whey) protein concentrate developed by Dr. Gustavo Bounous and others that supports glutathione (GSH) levels. GSH plays a key role in the immune system and defending against free radicals, infections, and toxins. The protein concentrate is rich in cystine and glutamylcystine, precursors of GSH that allow for sustained intracellular GSH levels. Clinical trials have shown it can boost the immune response and support cancer patients and those undergoing surgery or chemotherapy. The concentrate provides a stable delivery of cystine that is efficiently converted to cysteine inside cells for GSH production, maintaining optimal immune function.
LONG TERM ENTERAL GLTMN FOR BBLR IN AMKARA TURKISH (SUMMARY).pptx
Long-term enteral glutamine supplementation was shown to enhance growth in a time-dependent pattern in very low birth weight infants compared to controls based on a study of 69 infants. While growth parameters were similar in the first two months, the supplemented infants had significantly higher weight, length, head circumference, mid-arm circumference, and mid-thigh circumference at three and four months. No signs of glutamine toxicity were observed. The study suggests long-term glutamine supplementation may positively impact growth in preterm infants, but further research is still needed.
Nutrigenomics
Nutrigenomics is the science that examines the response of individuals to food compounds using post-genomic and related technologies (e.g. genomics, transcriptomics, proteomics, metabol/nomic etc.). The long-term aim of nutrigenomics is to understand how the whole body responds to real foods using an integrated approach termed 'systems biology'. The huge advantage in this approach is that the studies can examine people (i.e. populations, sub-populations - based on genes or disease - and individuals), food, life-stage and life-style without preconceived ideas.
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Protective Effect of Salacia Oblanga and Quercetin on Cyclophosphamide-Induce...International Journal of Engineering Inventions www.ijeijournal.com
This study investigated the protective effects of Salacia oblanga and quercetin on cyclophosphamide-induced chromosome aberrations in rat bone marrow cells. Rats were treated with Salacia oblanga or quercetin for 15 days, then given cyclophosphamide on days 14 and 15. Cyclophosphamide is known to induce chromosome aberrations and oxidative stress. The study found that quercetin completely prevented cyclophosphamide-induced chromosome aberrations, while Salacia oblanga partially prevented them. Both treatments decreased oxidative stress caused by cyclophosphamide. The results suggest that Salacia oblanga and quercetin can protect against the genotoxic and oxidative effects of cyclophosphFriedman o&p2013
Maternal high fat diet and obesity can negatively impact fetal development through several mechanisms. Studies in non-human primates found that offspring of mothers consuming a high fat diet exhibited fetal liver triglyceride accumulation and an elevated stress response in the liver as early as mid-gestation. A high fat diet was also found to impair placental function, increase inflammation in the maternal circulation, and elevate fetal cytokines. These findings suggest that excessive lipid exposure in utero, driven by maternal obesity and diet, can prime the fetus for later metabolic diseases like obesity and diabetes.
The effects of diethylstilbestrol administration on rat kidney. ultrastructur...
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This study examined the effects of diethylstilbestrol (DES) administration on rat kidney tissues over different time periods using histological, immunohistochemical, and ultrastructural analysis. Thirty male rats were divided into three groups: a control group, a group that received 60 μg/kg DES daily for 20 days, and a group that received the same dose of DES for 50 days. DES administration for 50 days caused degeneration of renal tissues, damage to renal tubules, increased cellularity of glomeruli, and a significant increase in BAX protein expression, indicating increased apoptosis. These changes were less pronounced after 20 days of treatment. The study found that non-steroidal synthetic estrogens like DES can haveAging and animal biotechnology
It is about aging and how aging is tried to controlled by different sort of methods and animals models are used in the testing the products created by science. It explains the different theories of aging in a very detailed manner. And the very least includes different animal models like mouse and monkey on which these treatments are applied and checked the effects of them that how we can control aging. We, can never say that controlling aging is something about that we are becoming immortal it is totally about finding the factors which can reduce tha aging and aging related diseases.
Lectins - The next medical tool?
Lectins are proteins or glycoproteins found ubiquitously in nature that bind specifically to carbohydrates. They have a variety of effects including agglutinating red blood cells, interfering with immune function, and altering cell surface proteins. Research suggests lectins show promise as therapeutic agents when used at proper dosages, as they demonstrate antiviral, antibacterial, and anticancer properties by inhibiting pathogen attachment or modifying cell surfaces. Lectins also show potential as diagnostic aids by binding differently to normal and abnormal cells. While lectins pose toxicity risks if not properly degraded or absorbed, their specific carbohydrate binding makes them useful for blocking pathogens, stimulating immunity, and modulating hormones and metabolism.
published journals
It appears that you have provided information about the "Indo-American Journal of Agricultural and Veterinary Sciences" . This journal seems to be an international online publication in English, published quarterly. It emphasizes fast publication while maintaining a rigorous peer-review process of the published journals.
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SPHHS Poster
- 1. INTRODUCTION HYPOTHESIS CONCLUSIONS * . METHODS RESULTS ACKNOWLEDGEMENTS Measuring Tissue-specific Glutathione (GSH) Utilization in the Developing Embryo Sarah Brown, Karen Melendez, Alicia Timme-Laragy • Humans are continuously exposed to oxidative stress-inducing environmental contaminants each day. • Embryonic development is extremely sensitive to oxidative stress due to the extreme changes in cell growth, development and differentiation that occur. • Therefore antioxidant functions during development are essential. • The most abundant antioxidant is glutathione (GSH). • Oxidation of GSH causes the formation of glutathione disulfide (GSSG), which can then be recycled back to GSH through the enzyme glutathione disulfide reductase. We aim to discover tissue-specific utilization of GSH to identify sensitive targets of oxidative stress during development No spatial analysis of GSH utilization has yet been performed in the zebrafish. Glutathione Synthesis GSH Redox Potential in the developing Zebrafish Embryo Timme-Laragy, Alicia R., et al. "Glutathione redox dynamics and expression of glutathione-related genes in the developing embryo." Free Radical Biology and Medicine 65 (2013): 89-101. Tissue-specific utilization of GSH will change during embryonic development. FUTURE DIRECTIONS • Tissue-specific utilization of GSH changes in the developing embryo. • The yolk sac retained consistently high levels of GSH throughout all developmental stages. • Other structures such as the eye, tail bud, somite 5, esophagus, heart and kidney region all displayed transient peaks depending on the developmental stage. • Do chemical exposures affect GSH distribution and utilization? • Can we predict target tissues that will be sensitive to oxidative stress during different stages of development? Members of the Timme-Laragy Lab: Dr. Karilyn Sant, Michelle Rousseau, Derek Luthi, Christopher Sparages, Katrina Borofski, Kaylee-Anna Williams, Shana Fleischman, Jiali Xu Department of Environmental Health Sciences, University of Massachusetts Amherst, Amherst MA 01003 Intensity(AU)Intensity(AU) Intensity(AU)Intensity(AU)Intensity(AU) Intensity(AU)Intensity(AU)Intensity(AU) Intensity(AU) Yolk Eye Tailbud Yolk Eye Tailbud Cerebellum Somite 1/2 Yolk Eye Tailbud Yolk Eye Pectoral Fin Bud Somite 5 Yolk Eye Somite 5 Aortic Arches Yolk Eye Somite 5 Heart Otolith Yolk Eye Swim Bladder Pharynx/ Esophagus Yolk Eye Intestine Stomach Eye (pupil) Gallbladder Kidney Region Liver ABSTRACT Humans are exposed to oxidative stress-inducing environmental contaminants every day. Embryonic development is extremely sensitive to oxidative stress due to the extreme changes in cell growth, development and differentiation that occur during this life stage. These changes depend on reduction and oxidation potentials that influence cell signaling pathways; therefore antioxidant functions during development are essential. The most abundant non-protein antioxidant is glutathione (GSH), however the development of GSH concentration during early life stages is still not well understood. The GSH S-transferase catalyzed reaction that occurs with the dye Monochlorobimane (MCB) and GSH creates a fluorescent adduct that can be seen and measured. Here, we propose that incubating zebrafish embryos (Danio rerio) of the Mitfa strain that lack pigment, at different developmental stages in .02 mM MCB will allow for a reliable method showing tissue-specific sensitivity to oxidative stress by observing critical windows of organ development and understand the mechanism as to how embryonic stages are effected by oxidative stress. We examined GSH concentrations at 6 developmental stages, using NAC and Menadione as models to manipulate GSH content in the embryos. Images were quantified using Image J. Here, stage specific staining of GSH showed that the certain organs using GSH change during development. The yolk sac retained consistently high levels of GSH throughout all developmental stages whereas the cerebellum, eye, and heart displayed transient peaks depending on the developmental stage. We conclude that MCB is a sufficient method in measuring tissue-specific GSH concentrations and oxidative stress during embryonic development. • T-tests and F-tests were performed using Excel to determine significance. Timme-Laragy, Alicia R., et al. "Glutathione redox dynamics and expression of glutathione-related genes in the developing embryo." Free Radical Biology and Medicine 65 (2013): 89-101. Bright Field Overlay Fluorescence Only Heat Map GST + GSH MCB Fluorescence