SlideShare a Scribd company logo
1 of 11
Download to read offline
Gwyneth Lorena Serna Salazar
Tercer semestre
Universidad Pontificia Bolivariana
Introduction
What is escherichia coli?
Escherichia coli (E. coli) is a bacterium that normally lives in our
intestines and those of other animals. Most of the time it does
not cause problems and, in fact, is useful in digestion and the
production of some vitamins. However, some strains of E. coli
can make us sick. These strains can cause gastrointestinal,
urinary tract, respiratory, wound and other infections. Some of
these strains produce toxins that can cause serious illness.
Genes mcr-4, mcr-5, mcr-6, and mcr-9
The genes mcr-4, mcr-5, mcr-6, and mcr-9
encode proteins that modify the
lipopolysaccharide in the outer membrane
of gram-negative bacteria, preventing
colistin from binding and exerting its
antibacterial effect. This modification
reduces the affinity of colistin for the
bacterial membrane, leading to bacterial
resistance to this antibiotic.
general objectives
1- Investigate the prevalence of antimicrobial-resistant
bacteria in broiler chickens in Malaysia.
2- Determine the antimicrobial resistance profiles of the
isolated bacteria.
3- Identify the antimicrobial resistance genes present in the
isolated bacteria.
Métodos
Capacidad más baja de agente
antimicrobiano necesaria para detener
el crecimiento de un microorganismo.
Importante para evaluar la
sensibilidad de un microorganismo y
dar un tratamiento adecuado.
Técnica molecular que amplifica
segmentos específicos de ADN,
permitiendo identificar los genes
relacionados con la resistencia a
antibióticos.
PCR MIC
Métodos
Capacidad de un microorganismo para
ser inhibido o eliminado por un
antimicrobiano. En este estudio se
utilizó para evaluar resistencia de las
cepas de E. Coli.
La MLST se usa para caracterizar
bacterias como E. coli resistentes a
colistina. Se identificaron linajes
principales y se estudió cómo se
dispersan en distintas fuentes, como
cloacas y agua potable.
MLST Susceptibilidad antimicrobiana
Resultados
Imagen de electroforesis en gel
recortada del producto PCR
amplificado de los genes Pho E. Coli
Imagen recortada de electroforesis en gel
del producto PCR amplificado con los
genes mcr-1, mcr-4 y mcr-5.
Resultados
Imagen recortada de electroforesis en
gel del producto PCR amplificado con
los genes mcr-6, mcr-8 y mcr-9.
Imagen de electroforesis en gel de agarosa
de la tipificación filogenética de E. coli mcr
positiva.
Discussion
Author what the author said agree/disagree
Hindler JA et al. The susceptibility test for colistin by disc
diffusion and E-test is difficult due to
polymyxins’ poor agar diffusion.
Zhang W et al.
Recently, following the ban of colistin use
as food additive in food animals in many
countries, the prevalence of mcr-1 gene
positive bacteria in food animals,
including chickens has decreased.
Liu YY et al. Nonetheless, PCR is widely considered as
the gold standard method in detecting
colistin-resistance.
Conclusions
1. Molecular biology, such as MLST, helps to track the spread of antimicrobial
resistance in bacteria such as E. coli.
2. Antimicrobial susceptibility is crucial for determining the efficacy of treatments
against resistant strains, which guides clinical decisions.
3. Molecular biology identifies resistance genes and characterizes resistant strains,
key to understanding and controlling antimicrobial resistance.
Mapa conceptual

More Related Content

Similar to Seminario Biomol- Gwyneth Lorena Serna Salazar

NetBioSIG2014-Keynote by Marian Walhout
NetBioSIG2014-Keynote by Marian WalhoutNetBioSIG2014-Keynote by Marian Walhout
NetBioSIG2014-Keynote by Marian WalhoutAlexander Pico
 
Fungal Contamination
Fungal ContaminationFungal Contamination
Fungal ContaminationAshley Lott
 
Summer Research Poster 2014 V.3.0
Summer Research Poster 2014 V.3.0Summer Research Poster 2014 V.3.0
Summer Research Poster 2014 V.3.0Ben Peterson
 
An Understanding Of Bacterial Transformation By Plasmid Dna
An Understanding Of Bacterial Transformation By Plasmid DnaAn Understanding Of Bacterial Transformation By Plasmid Dna
An Understanding Of Bacterial Transformation By Plasmid DnaGina Buck
 
Seminario biologia molecular - The correlation between biofilm formation capa...
Seminario biologia molecular - The correlation between biofilm formation capa...Seminario biologia molecular - The correlation between biofilm formation capa...
Seminario biologia molecular - The correlation between biofilm formation capa...IsabellaArcila
 
Comparison of carbon nutrition for pathogenic & commensal e. coli strains...
Comparison of carbon nutrition for pathogenic & commensal e. coli strains...Comparison of carbon nutrition for pathogenic & commensal e. coli strains...
Comparison of carbon nutrition for pathogenic & commensal e. coli strains...Andrew Fabich
 
Laboratory detection of resistant bacteria
Laboratory detection of resistant bacteriaLaboratory detection of resistant bacteria
Laboratory detection of resistant bacteriaMostafa Mahmoud
 
presentation on E.coli
presentation on E.colipresentation on E.coli
presentation on E.coliVandana singh
 
E. coli (Morphology, Growth Requirements and Genetics)
E. coli (Morphology, Growth Requirements and Genetics)E. coli (Morphology, Growth Requirements and Genetics)
E. coli (Morphology, Growth Requirements and Genetics)Siddhartha Dan
 

Similar to Seminario Biomol- Gwyneth Lorena Serna Salazar (13)

Esbl
EsblEsbl
Esbl
 
E. Coli
E. ColiE. Coli
E. Coli
 
NetBioSIG2014-Keynote by Marian Walhout
NetBioSIG2014-Keynote by Marian WalhoutNetBioSIG2014-Keynote by Marian Walhout
NetBioSIG2014-Keynote by Marian Walhout
 
Fungal Contamination
Fungal ContaminationFungal Contamination
Fungal Contamination
 
Summer Research Poster 2014 V.3.0
Summer Research Poster 2014 V.3.0Summer Research Poster 2014 V.3.0
Summer Research Poster 2014 V.3.0
 
Rloia Pickettiir
Rloia PickettiirRloia Pickettiir
Rloia Pickettiir
 
An Understanding Of Bacterial Transformation By Plasmid Dna
An Understanding Of Bacterial Transformation By Plasmid DnaAn Understanding Of Bacterial Transformation By Plasmid Dna
An Understanding Of Bacterial Transformation By Plasmid Dna
 
Seminario biologia molecular - The correlation between biofilm formation capa...
Seminario biologia molecular - The correlation between biofilm formation capa...Seminario biologia molecular - The correlation between biofilm formation capa...
Seminario biologia molecular - The correlation between biofilm formation capa...
 
Comparison of carbon nutrition for pathogenic & commensal e. coli strains...
Comparison of carbon nutrition for pathogenic & commensal e. coli strains...Comparison of carbon nutrition for pathogenic & commensal e. coli strains...
Comparison of carbon nutrition for pathogenic & commensal e. coli strains...
 
Laboratory detection of resistant bacteria
Laboratory detection of resistant bacteriaLaboratory detection of resistant bacteria
Laboratory detection of resistant bacteria
 
presentation on E.coli
presentation on E.colipresentation on E.coli
presentation on E.coli
 
E. coli (Morphology, Growth Requirements and Genetics)
E. coli (Morphology, Growth Requirements and Genetics)E. coli (Morphology, Growth Requirements and Genetics)
E. coli (Morphology, Growth Requirements and Genetics)
 
Seminario Biología Molecular
Seminario Biología MolecularSeminario Biología Molecular
Seminario Biología Molecular
 

Recently uploaded

Screening for colorectal cancer AAU.pptx
Screening for colorectal cancer AAU.pptxScreening for colorectal cancer AAU.pptx
Screening for colorectal cancer AAU.pptxtadehabte
 
Aditi Jagtap (Daughter of Ranjit Jagtap).pdf
Aditi Jagtap (Daughter of Ranjit Jagtap).pdfAditi Jagtap (Daughter of Ranjit Jagtap).pdf
Aditi Jagtap (Daughter of Ranjit Jagtap).pdfAditi Jagtap Pune
 
PNEUMOTHORAX AND ITS MANAGEMENTS.pdf
PNEUMOTHORAX   AND  ITS  MANAGEMENTS.pdfPNEUMOTHORAX   AND  ITS  MANAGEMENTS.pdf
PNEUMOTHORAX AND ITS MANAGEMENTS.pdfDolisha Warbi
 
SCHOOL HEALTH SERVICES.pptx made by Sapna Thakur
SCHOOL HEALTH SERVICES.pptx made by Sapna ThakurSCHOOL HEALTH SERVICES.pptx made by Sapna Thakur
SCHOOL HEALTH SERVICES.pptx made by Sapna ThakurSapna Thakur
 
Valproic Acid. (VPA). Antiseizure medication
Valproic Acid.  (VPA). Antiseizure medicationValproic Acid.  (VPA). Antiseizure medication
Valproic Acid. (VPA). Antiseizure medicationMohamadAlhes
 
Music Therapy's Impact in Palliative Care| IAPCON2024| Dr. Tara Rajendran
Music Therapy's Impact in Palliative Care| IAPCON2024| Dr. Tara RajendranMusic Therapy's Impact in Palliative Care| IAPCON2024| Dr. Tara Rajendran
Music Therapy's Impact in Palliative Care| IAPCON2024| Dr. Tara RajendranTara Rajendran
 
Systemic Lupus Erythematosus -SLE PT2.ppt
Systemic  Lupus  Erythematosus -SLE PT2.pptSystemic  Lupus  Erythematosus -SLE PT2.ppt
Systemic Lupus Erythematosus -SLE PT2.pptraviapr7
 
World-Health-Day-2024-My-Health-My-Right.pptx
World-Health-Day-2024-My-Health-My-Right.pptxWorld-Health-Day-2024-My-Health-My-Right.pptx
World-Health-Day-2024-My-Health-My-Right.pptxEx WHO/USAID
 
Study on the Impact of FOCUS-PDCA Management Model on the Disinfection Qualit...
Study on the Impact of FOCUS-PDCA Management Model on the Disinfection Qualit...Study on the Impact of FOCUS-PDCA Management Model on the Disinfection Qualit...
Study on the Impact of FOCUS-PDCA Management Model on the Disinfection Qualit...MehranMouzam
 
ANEMIA IN PREGNANCY by Dr. Akebom Kidanemariam
ANEMIA IN PREGNANCY by Dr. Akebom KidanemariamANEMIA IN PREGNANCY by Dr. Akebom Kidanemariam
ANEMIA IN PREGNANCY by Dr. Akebom KidanemariamAkebom Gebremichael
 
Prince Paulraj W HERBAL DRUG TECHNO.pptx
Prince Paulraj W HERBAL DRUG TECHNO.pptxPrince Paulraj W HERBAL DRUG TECHNO.pptx
Prince Paulraj W HERBAL DRUG TECHNO.pptxprincebieber28
 
Presentation on Non Steroidal Anti-inflammatory Agents
Presentation on Non Steroidal Anti-inflammatory AgentsPresentation on Non Steroidal Anti-inflammatory Agents
Presentation on Non Steroidal Anti-inflammatory AgentsPrerana Jadhav
 
Introduction to Sports Injuries by- Dr. Anjali Rai
Introduction to Sports Injuries by- Dr. Anjali RaiIntroduction to Sports Injuries by- Dr. Anjali Rai
Introduction to Sports Injuries by- Dr. Anjali RaiGoogle
 
Nutrition of OCD for my Nutritional Neuroscience Class
Nutrition of OCD for my Nutritional Neuroscience ClassNutrition of OCD for my Nutritional Neuroscience Class
Nutrition of OCD for my Nutritional Neuroscience Classmanuelazg2001
 
METHODS OF ACQUIRING KNOWLEDGE IN NURSING.pptx by navdeep kaur
METHODS OF ACQUIRING KNOWLEDGE IN NURSING.pptx by navdeep kaurMETHODS OF ACQUIRING KNOWLEDGE IN NURSING.pptx by navdeep kaur
METHODS OF ACQUIRING KNOWLEDGE IN NURSING.pptx by navdeep kaurNavdeep Kaur
 
Giftedness: Understanding Everyday Neurobiology for Self-Knowledge
Giftedness: Understanding Everyday Neurobiology for Self-KnowledgeGiftedness: Understanding Everyday Neurobiology for Self-Knowledge
Giftedness: Understanding Everyday Neurobiology for Self-Knowledgeassessoriafabianodea
 
PHYSIOTHERAPY IN HEART TRANSPLANTATION..
PHYSIOTHERAPY IN HEART TRANSPLANTATION..PHYSIOTHERAPY IN HEART TRANSPLANTATION..
PHYSIOTHERAPY IN HEART TRANSPLANTATION..AneriPatwari
 
Informed Consent Empowering Healthcare Decision-Making.pptx
Informed Consent Empowering Healthcare Decision-Making.pptxInformed Consent Empowering Healthcare Decision-Making.pptx
Informed Consent Empowering Healthcare Decision-Making.pptxSasikiranMarri
 
The next social challenge to public health: the information environment.pptx
The next social challenge to public health:  the information environment.pptxThe next social challenge to public health:  the information environment.pptx
The next social challenge to public health: the information environment.pptxTina Purnat
 
Plant Fibres used as Surgical Dressings PDF.pdf
Plant Fibres used as Surgical Dressings PDF.pdfPlant Fibres used as Surgical Dressings PDF.pdf
Plant Fibres used as Surgical Dressings PDF.pdfDivya Kanojiya
 

Recently uploaded (20)

Screening for colorectal cancer AAU.pptx
Screening for colorectal cancer AAU.pptxScreening for colorectal cancer AAU.pptx
Screening for colorectal cancer AAU.pptx
 
Aditi Jagtap (Daughter of Ranjit Jagtap).pdf
Aditi Jagtap (Daughter of Ranjit Jagtap).pdfAditi Jagtap (Daughter of Ranjit Jagtap).pdf
Aditi Jagtap (Daughter of Ranjit Jagtap).pdf
 
PNEUMOTHORAX AND ITS MANAGEMENTS.pdf
PNEUMOTHORAX   AND  ITS  MANAGEMENTS.pdfPNEUMOTHORAX   AND  ITS  MANAGEMENTS.pdf
PNEUMOTHORAX AND ITS MANAGEMENTS.pdf
 
SCHOOL HEALTH SERVICES.pptx made by Sapna Thakur
SCHOOL HEALTH SERVICES.pptx made by Sapna ThakurSCHOOL HEALTH SERVICES.pptx made by Sapna Thakur
SCHOOL HEALTH SERVICES.pptx made by Sapna Thakur
 
Valproic Acid. (VPA). Antiseizure medication
Valproic Acid.  (VPA). Antiseizure medicationValproic Acid.  (VPA). Antiseizure medication
Valproic Acid. (VPA). Antiseizure medication
 
Music Therapy's Impact in Palliative Care| IAPCON2024| Dr. Tara Rajendran
Music Therapy's Impact in Palliative Care| IAPCON2024| Dr. Tara RajendranMusic Therapy's Impact in Palliative Care| IAPCON2024| Dr. Tara Rajendran
Music Therapy's Impact in Palliative Care| IAPCON2024| Dr. Tara Rajendran
 
Systemic Lupus Erythematosus -SLE PT2.ppt
Systemic  Lupus  Erythematosus -SLE PT2.pptSystemic  Lupus  Erythematosus -SLE PT2.ppt
Systemic Lupus Erythematosus -SLE PT2.ppt
 
World-Health-Day-2024-My-Health-My-Right.pptx
World-Health-Day-2024-My-Health-My-Right.pptxWorld-Health-Day-2024-My-Health-My-Right.pptx
World-Health-Day-2024-My-Health-My-Right.pptx
 
Study on the Impact of FOCUS-PDCA Management Model on the Disinfection Qualit...
Study on the Impact of FOCUS-PDCA Management Model on the Disinfection Qualit...Study on the Impact of FOCUS-PDCA Management Model on the Disinfection Qualit...
Study on the Impact of FOCUS-PDCA Management Model on the Disinfection Qualit...
 
ANEMIA IN PREGNANCY by Dr. Akebom Kidanemariam
ANEMIA IN PREGNANCY by Dr. Akebom KidanemariamANEMIA IN PREGNANCY by Dr. Akebom Kidanemariam
ANEMIA IN PREGNANCY by Dr. Akebom Kidanemariam
 
Prince Paulraj W HERBAL DRUG TECHNO.pptx
Prince Paulraj W HERBAL DRUG TECHNO.pptxPrince Paulraj W HERBAL DRUG TECHNO.pptx
Prince Paulraj W HERBAL DRUG TECHNO.pptx
 
Presentation on Non Steroidal Anti-inflammatory Agents
Presentation on Non Steroidal Anti-inflammatory AgentsPresentation on Non Steroidal Anti-inflammatory Agents
Presentation on Non Steroidal Anti-inflammatory Agents
 
Introduction to Sports Injuries by- Dr. Anjali Rai
Introduction to Sports Injuries by- Dr. Anjali RaiIntroduction to Sports Injuries by- Dr. Anjali Rai
Introduction to Sports Injuries by- Dr. Anjali Rai
 
Nutrition of OCD for my Nutritional Neuroscience Class
Nutrition of OCD for my Nutritional Neuroscience ClassNutrition of OCD for my Nutritional Neuroscience Class
Nutrition of OCD for my Nutritional Neuroscience Class
 
METHODS OF ACQUIRING KNOWLEDGE IN NURSING.pptx by navdeep kaur
METHODS OF ACQUIRING KNOWLEDGE IN NURSING.pptx by navdeep kaurMETHODS OF ACQUIRING KNOWLEDGE IN NURSING.pptx by navdeep kaur
METHODS OF ACQUIRING KNOWLEDGE IN NURSING.pptx by navdeep kaur
 
Giftedness: Understanding Everyday Neurobiology for Self-Knowledge
Giftedness: Understanding Everyday Neurobiology for Self-KnowledgeGiftedness: Understanding Everyday Neurobiology for Self-Knowledge
Giftedness: Understanding Everyday Neurobiology for Self-Knowledge
 
PHYSIOTHERAPY IN HEART TRANSPLANTATION..
PHYSIOTHERAPY IN HEART TRANSPLANTATION..PHYSIOTHERAPY IN HEART TRANSPLANTATION..
PHYSIOTHERAPY IN HEART TRANSPLANTATION..
 
Informed Consent Empowering Healthcare Decision-Making.pptx
Informed Consent Empowering Healthcare Decision-Making.pptxInformed Consent Empowering Healthcare Decision-Making.pptx
Informed Consent Empowering Healthcare Decision-Making.pptx
 
The next social challenge to public health: the information environment.pptx
The next social challenge to public health:  the information environment.pptxThe next social challenge to public health:  the information environment.pptx
The next social challenge to public health: the information environment.pptx
 
Plant Fibres used as Surgical Dressings PDF.pdf
Plant Fibres used as Surgical Dressings PDF.pdfPlant Fibres used as Surgical Dressings PDF.pdf
Plant Fibres used as Surgical Dressings PDF.pdf
 

Seminario Biomol- Gwyneth Lorena Serna Salazar

  • 1. Gwyneth Lorena Serna Salazar Tercer semestre Universidad Pontificia Bolivariana
  • 2. Introduction What is escherichia coli? Escherichia coli (E. coli) is a bacterium that normally lives in our intestines and those of other animals. Most of the time it does not cause problems and, in fact, is useful in digestion and the production of some vitamins. However, some strains of E. coli can make us sick. These strains can cause gastrointestinal, urinary tract, respiratory, wound and other infections. Some of these strains produce toxins that can cause serious illness.
  • 3. Genes mcr-4, mcr-5, mcr-6, and mcr-9 The genes mcr-4, mcr-5, mcr-6, and mcr-9 encode proteins that modify the lipopolysaccharide in the outer membrane of gram-negative bacteria, preventing colistin from binding and exerting its antibacterial effect. This modification reduces the affinity of colistin for the bacterial membrane, leading to bacterial resistance to this antibiotic.
  • 4. general objectives 1- Investigate the prevalence of antimicrobial-resistant bacteria in broiler chickens in Malaysia. 2- Determine the antimicrobial resistance profiles of the isolated bacteria. 3- Identify the antimicrobial resistance genes present in the isolated bacteria.
  • 5. Métodos Capacidad más baja de agente antimicrobiano necesaria para detener el crecimiento de un microorganismo. Importante para evaluar la sensibilidad de un microorganismo y dar un tratamiento adecuado. Técnica molecular que amplifica segmentos específicos de ADN, permitiendo identificar los genes relacionados con la resistencia a antibióticos. PCR MIC
  • 6. Métodos Capacidad de un microorganismo para ser inhibido o eliminado por un antimicrobiano. En este estudio se utilizó para evaluar resistencia de las cepas de E. Coli. La MLST se usa para caracterizar bacterias como E. coli resistentes a colistina. Se identificaron linajes principales y se estudió cómo se dispersan en distintas fuentes, como cloacas y agua potable. MLST Susceptibilidad antimicrobiana
  • 7. Resultados Imagen de electroforesis en gel recortada del producto PCR amplificado de los genes Pho E. Coli Imagen recortada de electroforesis en gel del producto PCR amplificado con los genes mcr-1, mcr-4 y mcr-5.
  • 8. Resultados Imagen recortada de electroforesis en gel del producto PCR amplificado con los genes mcr-6, mcr-8 y mcr-9. Imagen de electroforesis en gel de agarosa de la tipificación filogenética de E. coli mcr positiva.
  • 9. Discussion Author what the author said agree/disagree Hindler JA et al. The susceptibility test for colistin by disc diffusion and E-test is difficult due to polymyxins’ poor agar diffusion. Zhang W et al. Recently, following the ban of colistin use as food additive in food animals in many countries, the prevalence of mcr-1 gene positive bacteria in food animals, including chickens has decreased. Liu YY et al. Nonetheless, PCR is widely considered as the gold standard method in detecting colistin-resistance.
  • 10. Conclusions 1. Molecular biology, such as MLST, helps to track the spread of antimicrobial resistance in bacteria such as E. coli. 2. Antimicrobial susceptibility is crucial for determining the efficacy of treatments against resistant strains, which guides clinical decisions. 3. Molecular biology identifies resistance genes and characterizes resistant strains, key to understanding and controlling antimicrobial resistance.