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Seman Analysis
part -3
Seman Macroscopic
Examination
Medical technologist: Zubair Ahmed
Motivational Science T.V💉
Initial macroscopic examination:
•Liquefaction
1. Within a few minutes at room temperature, the semen usually begins to liquefy
2. Normal liquefied semen samples may contain jelly-like granules (gelatinous bod-
ies)
•Semen viscosity
After liquefaction, the viscosity of the sample can be estimated by gently aspirat- ing it into a
wide-bore (approximately 1.5 mm diameter) plastic disposable pipette, allowing the semen to
drop by gravity and observing the length of any thread.
•If viscosity is abnormal, the drop will form a thread more than 2 cm long.
: High viscosity can interfere with determination of sperm motility, sperm concentration, detection of antibody-
coated spermatozoa and measurement of biochemical markers
sample usually
liquefies within 15
minutes at room
temperature
•Appearance of the ejaculate
•A normal liquefied semen sample has a homogeneous, grey-opalescent appear- ance
•It may appear less opaque if the sperm concentration is very low
•red-brown when red blood cells are present (haemo- spermia),
•yellow in a man with jaundice or taking certain vitamins
•Semen volume:
The volume of the ejaculate is contributed mainly by the seminal vesicles and prostate gland, with
a small amount from the bulbourethral glands and epididy- mides
• A normal amount is between 2 and 5 milliliters
•Hypospermia is when the volume is less than 1.5 ml,
• Hyperspermia is a large volume of more than 5.5 ml.
•Semen pH
•The pH of semen reflects the balance between the pH values of the different accessory gland
secretions
•mainly the alkaline seminal vesicular secretion and the acidic prostatic secretion
•The pH should be measured after liquefaction at a uniform time, preferably after 30 minutes, but
in any case within 1 hour of ejacula- tion since it is influenced by the loss of CO2 that occurs after
production
•For normal samples, pH paper in the range 6.0 to 10.0 should be used.
•Mix the semen sample well (see Box 2.3).
•Spread a drop of semen evenly onto the pH paper.
•Wait for the colour of the impregnated zone to become uniform (<30 seconds).
•Compare the colour with the calibration strip to read the pH.

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Seman analysis part 3

  • 1. Seman Analysis part -3 Seman Macroscopic Examination Medical technologist: Zubair Ahmed Motivational Science T.V💉
  • 2. Initial macroscopic examination: •Liquefaction 1. Within a few minutes at room temperature, the semen usually begins to liquefy 2. Normal liquefied semen samples may contain jelly-like granules (gelatinous bod- ies) •Semen viscosity After liquefaction, the viscosity of the sample can be estimated by gently aspirat- ing it into a wide-bore (approximately 1.5 mm diameter) plastic disposable pipette, allowing the semen to drop by gravity and observing the length of any thread. •If viscosity is abnormal, the drop will form a thread more than 2 cm long. : High viscosity can interfere with determination of sperm motility, sperm concentration, detection of antibody- coated spermatozoa and measurement of biochemical markers sample usually liquefies within 15 minutes at room temperature
  • 3. •Appearance of the ejaculate •A normal liquefied semen sample has a homogeneous, grey-opalescent appear- ance •It may appear less opaque if the sperm concentration is very low •red-brown when red blood cells are present (haemo- spermia), •yellow in a man with jaundice or taking certain vitamins •Semen volume: The volume of the ejaculate is contributed mainly by the seminal vesicles and prostate gland, with a small amount from the bulbourethral glands and epididy- mides • A normal amount is between 2 and 5 milliliters •Hypospermia is when the volume is less than 1.5 ml, • Hyperspermia is a large volume of more than 5.5 ml.
  • 4. •Semen pH •The pH of semen reflects the balance between the pH values of the different accessory gland secretions •mainly the alkaline seminal vesicular secretion and the acidic prostatic secretion •The pH should be measured after liquefaction at a uniform time, preferably after 30 minutes, but in any case within 1 hour of ejacula- tion since it is influenced by the loss of CO2 that occurs after production •For normal samples, pH paper in the range 6.0 to 10.0 should be used. •Mix the semen sample well (see Box 2.3). •Spread a drop of semen evenly onto the pH paper. •Wait for the colour of the impregnated zone to become uniform (<30 seconds). •Compare the colour with the calibration strip to read the pH.