This document provides an overview of bioprinting technology and its applications in education. It summarizes the history of bioprinting from its origins in 1983 to recent developments. It then describes how bioprinters work and the various biomaterials and cell types that can be used for bioprinting. Examples of potential bioprinting applications are given across various industries. The remainder of the document focuses on using bioprinting technology in education, describing classroom bioprinters, bioinks, enzyme applications, and sample lab activities and curriculum tracks related to bioprinting.

1. SE3D Education
Bioprinting for next generation STEM students

2. History of Bioprinting
1983 – Charles Hull invented stereolithography
1990 – Gabor Forgacs explored the use of 3D printing
into tissue engineering
1999 – Wake Forest Institute of Regenerative Medicine
used a 3D printer to build a synthetic scaffold of
a human bladder
2003 – Inkjet printer used to deposit biological
materials (Thomas Boland)
2007 – Organovo, the first bioprinting company, was
founded
2009 – The first commercial bioprinter, NovoGen MMX
was introduced
From CAD to Object
3D printing
Bioprinting
© Copyright 2016 SE3D Education

3. How does a bioprinter work?
z
x y
Bio-ink
Bioprinting process
polymer
hydrogel
cells
in vitro tissue
Bioprinting
© Copyright 2016 SE3D Education

4. BIOPRINTING APPLICATIONS
Bioprinted
Organs
Medical
Devices
Artificial
Tissue
Biofuels
Drug
Discovery
Meat
Production
Introduction
© Copyright 2016 SE3D Education

5. r3bEL – The Classroom Bioprinter
1. Engage students in exciting and
cutting-edge science
2. Increase hands-on learning for
every student
3. Provide opportunities for students
to innovate in the biosciences
© Copyright 2016 SE3D Education

6. Bioink: hydrogels for bioprinting
Alginate gelation
Drug delivery Wound dressing
Hydrogels are network of polymer chains
containing >90% water.
• Natural
- alginate, agarose, collagen
• Synthetic
- pAAm, PEG
• Preparation: chemical / thermal crosslinking
Applications:
Bioink
© Copyright 2016 SE3D Education

7. Enzymes and their applications
What are enzymes?
Enzymes are proteins that act as catalysts and
facilitate complex biochemical reactions
Applications:
Screening for kinase inhibitors as
potential cancer drugs
Introduction to Enzymes
ABTS ABTS* (oxidized)
(substrate) (Blue product)
HRP (enzyme)
Example:
HER2 kinase inhibitors
reduce the overall HER2
activity &tumor growth
© Copyright 2016 SE3D Education

8. Today’s activity: Bioprinted enzyme arrays
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Top view
Side viewR3BEL Side view
Top view
Step 1: Setup printer Step 2: Print enzymes Step 3: Print substrate Step 4: See reaction!!
Lab activity
© Copyright 2016 SE3D Education

9. Other Workshops for Teachers
Our Commitment to Teachers
Enzyme Lab
Algae Lab
E. Coli Lab
Chocolate Printing
3D Modeling
- Creating enzyme arrays to learn more
about how enzymes work
- Creating algae arrays to understand
photosynthesis
- Creating bacteria arrays to understand
how antibiotics are developed
- Printing chocolate to learn more about
chocolate chemistry
- 3D tools for creating models for printing
For more information about our workshops, visit us at http://www.se3d.com/workshops.html

10. Bioprinted algae arrays
RESULTS
Lab activity
DATA ANALYSIS
© Copyright 2016 SE3D Education

11. Be in the Forefront of Classroom
Technology Innovation
Bioprinter: $2,500 (pilot)
• Compact and lightweight
• Heated platform 25 – 80 °C
• Heated extruder 25 – 100 °C
• Syringe volume 5 mL
• Syringe needle sizes 14 – 28 G
Available for one-year minimum
lease $95/month
1. BioTech Starter Kit
• Alginate
• HRP (enzyme)
• ABTS/H2O2 (substrates)
2. GreenTech Starter Kit
• Agarose
• Algae
• Bicarbonate indicator
Each kit makes 25-100 experiments
BioKits: $75-250
To request more information or a quotation, please email us info@se3d.com

12. SE3D Bioprinting Curriculum Tracks
BIOPRINTING LAB MODULES
Track II
Green Technology
Track I
Pharmaceutical
Track III
Tissue Engineering
EnzymesBasics AlgaeProteins Bacteria
Curriculum
For more information about our curriculum, email us info@se3d.com

13. Pilot School Program
Spring/Fall 2016
• 25% on bioprinter purchase ($2500 only)
• Exclusive preview of full curriculum
• 10% discount on first year curriculum
• Publicity and media coverage via SE3D
marketing/PR campaigns
• Free consultation of personalized learning
• Invitation of our bioprinting conference 2017
• Be the school that LEADS
To participate in our pilot program, email Dr. Maya Lim directly mlim@se3d.com