This document provides an overview of how various infectious agents can impair human reproductive function in both males and females. It discusses how bacteria, fungi, viruses and parasites can infect and damage different parts of the male and female reproductive tracts, potentially leading to infertility. Specific microorganisms that can cause reproductive tract infections are described in detail, including how they may interfere with fertility through direct damage to reproductive organs or sperm/egg function. The complex relationships between infections and infertility are also addressed.
Contents :
Amyloid
Apoprotein
Arthritis
Avascular necrosis
Bone graft
Brain
Bronchogenic carcinoma
Census
Clotting factors
Contraception
Inflammatory bowel disease
Cerebrospinal fluid
Culture media
Dementia
Electroencephalogram
Engaging diameter
Estrogen
Familial hyperlipoproteinemia
Features of fibroid
Firearm
Gastric motility
Absorption in gut and nephron
Hormones
Hypersensitivity
Immunoglobulin
Inborn errors of metabolism and deficient enzymes
Kidney stones
Lithium
Lung volumes and capacities
Metabolic acidosis
Mosquito
Nerve fibers
Muscles and nerve supply
Changes in pregnancy
Methods for proteins
Protooncogenes and tumor suppressor genes
Radioisotopes
Rickettsial zoonoses
Root values for reflexes
Rapidly progressing glomerulonephritis
Small intestinal biopsy
Specimen preservation in poisoning
Sexually transmitted diseases
Sterilization and disinfection
Tetanus
Toxins in food
Management of poisoning
Vaccination
Vaginal cytology
Vitamins
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You can send your queries to medpgnotes@gmail.com
Contents :
General features of pathology
Features of cell injury
Hypoxia
Ageing
Necrosis
General features of apoptosis
Apoptotic and anti apoptotic protein
Calcification
Atrophy and hypertrophy
Hyperplasia and metaplasia
Stem cells
Fixatives and stains
Pigment
Bactericidal system
Hydrogen peroxidase
Oxidative stress
Free radical
NADPH oxidase
Basement membrane
Inflammation
Inflammatory mediators
Hydrostatic and osmotic pressure
General features of inflammation
Systemic inflammatory response syndrome
Autoantigen and associated diseases
Acute inflammation
Chronic inflammation
Chronic granulomatous disease
Granuloma
Complement system
Opsonization
Phagocytosis
Chediak higashi syndrome
Chemotaxis
Neoplasia
Cell cycle
Causes of neoplasia
Features of neoplasia
Protooncogenes and tumor suppressor genes
Management of neoplasia
General features of tumor markers
CA-125
CEA
AFP
Features of tumors
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You can send your queries to medpgnotes@gmail.com
this is a series of notes on general pathology, useful for undergraduate and post graduate pathology students. Notes have been prepared from standard textbooks and are in a format easy to reproduce in exams.
Tuberculosis (TB) is a very common disease worldwide including India. Tuberculosis of the female genital tract is
common enough to be found in 1% of women with DUB (Sutherland 1949) and in 4% of adolescent with excessive
menstrual loss (Sutherland 1953). The commonest site of involvement is the fallopian tubes (90e100%). The next
common site is endometrium (60%). The infection is from the tubes either by lymphatics or direct spread through
continuity. Symptoms vary according to the severity site and stage of the disease. Anti tuberculosis chemotherapy is the mainstay of tt. Initially drugs are used for 2 months. These are isoniazid, rifampicin, pyrazinamide and ethambutal. Treatment is continued for another 4 months with isoniazid and rifampicin.
Infection in the couple may lead to failure of fertility treatment ,hence proper screening and management of the condition is mandatory before starting fertility treatment.
Oral-systemic link has been termed Periodontal Medicine. Significance: Periodontal disease is preventable and readily treatable, thus providing many new opportunities for preventing and improving several systemic diseases.
FOCAL INFECTION: Localized or Generalized infection caused by dissemination of microorganisms or toxic products from focus of infection.
FOCUS OF INFECTION Confined area that
(1) contains pathogenic microorganisms
(2) can occur anywhere in body
Diseases/Conditions affected by periodontitis
A PREGNANCY, PREECLAMPSIA
B ISCHEMIC HEART DISEASES, STROKE
C DIABETES MELLITUS
D PNEUMONIA, COPD
E OSTEOPOROSIS
F CANCER
G ALZHEIMER’S DISEASE
H. RHEUMATOID ARTHRITIS
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The people of Punjab felt alienated from main stream due to denial of their just demands during a long democratic struggle since independence. As it happen all over the word, it led to militant struggle with great loss of lives of military, police and civilian personnel. Killing of Indira Gandhi and massacre of innocent Sikhs in Delhi and other India cities was also associated with this movement.
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Dear Dr. Kornbluth and Mr. Gorenberg,
The US House of Representatives is deeply concerned by ongoing and pervasive acts of antisemitic
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1. MHR lecture Notes
Dr. Getso MI
Dept of Medical Microbiology & Parasitology,
Bayero University Kano.
2. OVERVIEW
Infectious agents can impair various important human
functions, including reproduction.
Bacteria, fungi, viruses and parasites are able to interfere
with the reproductive function in both sexes.
Infections of male genito-urinary tract account for about
15% of the case of male infertility.
These infections can affect different sites of the male
reproductive tract and spermatozoa themselves
3. In female, microorganisms are certainly involved
in cervical, tubal, and peritoneal damage,
Tubo-peritoneal damage seems to be the
foremost manner in which microorganisms
interfere with human fertility.
4. OVERVIEW
Reproductive tract infections (RTIs) include three types of infection:
o Sexually transmitted diseases (STDs), such as chlamydia,
gonorrhea, chancroid, and human immunodeficiency virus (HIV);
o Endogenous infections, which are caused by overgrowth of
organisms normally present in the genital tract of healthy women,
such as bacterial vaginosis or vulvovaginal candidiasis;
o Iatrogenic infections, which are associated with improperly
performed medical procedures such as unsafe abortion or poor
delivery practices (1). RTIs are preventable, and many are treatable
as well.
5. OVERVIEW
“Infertility can be defined as the lack of a conception after
at least one year of constant, unprotected vaginal sexual
intercourse, in the absence of known reproductive
pathology”
NICE guideline, CG156
The underlying causes may be ascribed to pathologic
conditions affecting one or both members of a couple
6. OVERVIEW
Intracellular
Energy parasites
Elementary body
Reticulate body
Inclusion bodies – HP, LCL
Biotypes/ serotypes
Tissue culture
C. trachomatis
Trachoma
Inclusion conjunctivitis
Lymphogranuloma venereum
Frei test
Genital chlamydiasis
C. psittaci
Psittacosis
Ornithosis
C. pneumoniae
7. Infertility and RTIs
There is a complex relationship between infections and
infertility.
Many microorganisms seem to be involved in male
reproductive failure in various ways, and to different
degrees of statistical association.
Infections of male lower genital tract seem to have little
importance, if not, of an occlusion.
Infections involving other parts of the male GUT, may
cause a microbial colonization of the semen.
8. Infertility and RTIs……
Virtually all parts of the female reproductive system may be
influenced by infectious agents
Vaginal infections and cases of endometritis, leading to uterine
sinechiae, are less common than tubal occlusions resulting
from salpingitis.
Adhesions, caused by PID, seem to affect the functional
status of the tubes more harshly than that of the uterus.
9. Bacteria: Genital mycoplasmas
Mycoplasmas:
These microorganisms are frequently present in the
genital tract and semen of both fertile and infertile men
particularly true for Ureaplasma urealyticum, and
slightly for both Mycoplasma hominis and Mycoplasma
genitalium.
Ureaplasmas are a cause of nonchlamydial, non-
gonococcal urethritis (NGU) in men.
10. Mycoplasmas
Overnight co-incubation with M. hominis can produce
small but statistically significant differences in motility,
morphology, and fertilization potential in human
spermatozoa.
U. urealyticum is associated with the production of ROS,
even in absence of leucocytospermia.
M. genitalium can attach to human spermatozoa, and thus
could be carried out by motile sperm, causing female
genital diseases and infertility.
11. Mycoplasmas
In female, M. genitalium is strongly associated with
cervicitis, endometritis and serologically with
salpingitis-PID complex, and may account for a
number of cases of infertility .
Antibodies against this pathogen were found in
patients with tubal factor infertility (TFI), even in
cases where the presence of Chlamydia trachomatis
was excluded.
Diagnosed by isolation , DNA Probe, PCR
12. Neissseria gonorrhoeae
This bacterium is known to cause urethritis
Its incidence in the western world has diminished over the last two
decades, as a result of more accurate sexual hygiene following the
onset (HIVAIDS) epidemic.
Gonococcal infections in women are believed to be in decline with
encouraging evidence even from Africa.
The primary gonococcal infection is present in the endocervix, with
concomitant urethral infection.
Ascending infection may occur in 10–20% of infected women and
can result in acute PID that may manifest as salpingitis,
endometritis, tubo-ovarian abscess, all of which can lead to
scarring, ectopic pregnancy, sterility, and chronic pelvic pain
13. Neissseria gonorrhoeae….
N. gonorrhoeae incubated with tubal epithelial cell
produced IL-1α, IL-β and TNF-α suggested the genesis
of gonoccus-induced infertility.
Diagnosis is by Gram smear of urethral discharge (male)
or EC specimen in symptomatic female; GNC in pairs
associated with PMNs
supported by immunological or molecular procedures.
14. Chlamydia trachomatis
C. trachomatis has a worldwide distribution, affecting both
sexes but has a much greater impact on females than on
males.
It particularly affects young women and sexually active
adolescents
Serovar D-K causes NGU, epididymitis, prostatitis,
cervicitis, urethritis, endometritis, salpingitis, and
perihepatitis in women.
Induces Reiter’s syndrome, proctitis, and conjunctivitis in
both sex
16. Human diseases
Species Serotype Disease
C. trachomatis A, B, Ba, C Endemic blinding
trachoma
C. trachomatis D to K Inclusion conjunctivitis.
Genital chlamydiasis
C. trachomatis L1, L2, L3 Lymphogranuloma
venereum
C. psittaci Many serotypes Psittacosis
C. pneumoniae Acute resp. disease
17. C. trachomatis….
C. trachomatis, when co-incubated with human
spermatozoa in vitro, seems to impair sperm motility, and
cause premature death, perhaps as an effect of the
chlamydial lipopolysaccaride
Infection by C. trachomatis is related to the production of
anti-sperm antibodies (ASA), but the extent to which
ASAs actually affect fertility is yet to be clarified
There is a clear relationship between male infection,
which is often asymptomatic and the possibility of
transmission to the more susceptible female partner
18. C. trachomatis….
In female, infections interfere seriously with human
reproduction.
Cause of tubal obstructions, lacerations and ectopic
pregnancy, and can result in PID, adnexitis, local or
diffuse peritonitis, and formation of adhesions.
C. trachomatis is believed to be one of the major causes
of cervical factor infertility (CFI), as a result of the
alterations of the epithelium and mucus composition, and
by the presence of inflammatory cells
19. C. trachomatis….
The chlamydial 10 kDa and 57 kDa HSP (cHSP10 and
cHSP57/60, respectively) show an amazing analogy to
human proteins.
Thus, there formation of antibodies against the HSP60 in
the serum and follicular fluid of women which have
negative impact on embryonal growth, and increase risk of
adverse pregnancy outcomes
antibodies against HSP60 and positive for C. trachomatis
seem to have a greater probability of tubal scaring and
ectopic pregnancy, compared to women only seropositive.
20. C. trachomatis….
There also seems to be a cross-reactivity btw HSP10 and
an embryonic protein, the early pregnancy factor (EPF),
and this may cause abortions.
cHSP10 too, probably correlate to the severity of the
disease in females and with the presence TFI. (La verda et al, 2008)
HSP57/60 also has been found to induce trophoblast
apoptosis by stimulating the TLR4, which naturally
mediates immune responses in placenta. (Equilis et al, 2006)
21. C. trachomatis….
Genetic conditions may have an important role in the
whole process of C. trachomatis-induced damage.
Some variants of the gene that controls IL-10 production
may be involved in the impairment of the immune
response against the bacterium
Although there is no absolute ‘‘gold standard’’ for
chlamydia tests, NA amplification (PCR, LCR, TMP)
assays show sensitivity ≥90% compared with 60–70%
for culture and 60% for antigen assays.
22. Enterobacteriaceae
E. coli is the most common cause of nonsexually
transmitted epididymo-orchitis and is involved in about
80% of total acute or chronic prostatitis.
E. coli may, therefore, be implicated in the genesis of
infertility, and the same might be true for other
enterobacteria belonging to the genera Klebsiella,
Salmonella and Proteus.
Ps. aeruginosa can cause epididymitis and prostatitis, and
may, in this manner, interfere with male fertility. (Weidner et al, 1999)
23. Gram-positive cocci
Streptococci and Staphylococci normally colonize the male
urethra, whilst Enterococci seem to be more frequently
present in the seminal tract.
All these genera seem to be implicated in the genesis of
prostatitis and epididymitis, and thus may impair male
fertility.
Enterococci are often present in the semen of infertile
men, and their presence is associated with impaired
seminal parameters
24. Gram-positive cocci….
Streptococci and Staphylococci too are often present
in the urethra of infertile men, but unlike Enterococci,
do not seem to impair semen quality .
Mehta et al. mention an association between the
presence of Enterococcus, oligospermia and
teratospermia. (Mehta et al,2002)
25. Gardnerella vaginalis
G. vaginalis seems to be very common in the genital tract
of men with suspected infertility, and is also frequent
among infertile men and even more among their wives
Common agent associated with bacterial vaginosis, apart
from M. hominis and U. urealyticum, Mobiluncus spp.,
Bacteroides spp. and peptostreptococcus spp. Are also
common
Ascending dissemination of the involved species may lead
to tubal factor infertility
26. Helicobacter pylori
H. pylori may be the only microorganism to impair fertility
without infecting the genital tract or its glandular
structures.
H. pylori was significantly more prevalent among infertile
men and women than among healthy controls
Antibodies against H. pylori were found in genital fluids of
infertile patients (100% of follicular fluids, 50% of sperm
samples, a minority of vaginal secretions)
Anti-helicobacter Atb cross-react with the tails and the
pericentriolar area of human spermatozoa. ??
27. Yeasts
Candida albicans commonly colonizes the urethra, but
rarely the accessory glands.
C. albicans, as well as its filtrates, have an inhibitory
effect on human sperm motility, and impaired the
ultrastructure of human spermatozoa, which could be
associated with male infertility
C. albicans is a common commensal of the female genital
tract and rarely cause condition more serious than
recurrent vulvovaginal andidiasis (RVVC) in females.
28. Parasites and other rare conditions
Trichomonas vaginalis has a worldwide distribution and it
affects both sexes
In males, it is a rare cause a NGU and perhaps prostatitis
Proteinases released by T. vaginalis can also inhibit sperm
motility in vitro, even after the microorganism has been
removed from the culture medium
The parasite is frequently present within infertile couples,
particularly among women.
29. T. vaginalis
The clear association btw the presence of T. vaginalis and
bacterial vaginosis
Trichomoniasis is assoc with mild vaginal and cervical
damage, and doesnt seem capable of producing CFI.
T. vaginalis is assoc decreased C3 and C4 complement
factors, increased IgA level in vaginal discharge, and
increased serum prolactin.
This protozoan disease has also been associated with
adverse pregnancy outcomes
30. T. vaginalis….
T. vaginalis promotes the action of M. hominis, by
transporting the bacterium inside the protozoa cell.
This offers protection from the action of the immune
system and the effects of therapies, and favors its spread
through the genital tract,
31. Other protozoarian diseases have been associated with
impaired fertility
Genital amoebiasis can seriously damage female
reproductive system and cause sterility.
Toxoplasmosis may give infertility by producing ASA, or
by causing hypogonadotropic hypogonadism.
Genital tuberculosis, leprosy, and helminthes (eg Filarisis)
may damage the testes and impair male fertility.
32. Viruses
HSV infection of the male genital tract is implicated with
male infertility, as it is associated with decreased semen
quality
The presence of HSV DNA in human spermatozoa has
been proven
Antiviral treatment of male infertility patients positive for
HSV in semen resulted in successful pregnancies
HSV infections, on the other hand, seem to have no
significant association with cervical factor infertility
33. Human papilloma viruses (HPV)
HPV were found in testicular biopsies of azoospermic
men.
when present inside sperm cells, they may be related to
impaired sperm motility and asthenozoospermia.
Fernandez et al. found, in 1998, an association between
HPV and infertility, particularly TFI
34. Human Immunodeficiency Viruses
(HIV)
HIV may impair semen parameters by itself and certainly
deteriorates the outcome of concomitant genital infections
HIV has a negative impact also on female fertility, but it is
not clear to what extent this is due: to the activity of the
virus itself, or to other concomitant genital infections, or
even to drug side effects
37. References
Martinez-Garcia F, Regadera J, Mayer R, Sanchez S, Nistal M.Protozoan infections in the
male genital tract. J Urol 1996;156:340–9.
Richens J. Genital manifestations of tropical diseases. Sex Transm Infect 2004;80:12–7
Mehta RH, Sridhar H, Vijay-Kumar BR, Anand-Kumar TC. High incidence of oligozoospermia
and teratozoospermia in human semen infected with the aerobic bacterium Streptococcus
faecalis. Reprod Biomed Online 2002;5:17–21.
WeidnerW, KrauseW, Ludwig M. Relevance of male accessory gland infection for
subsequent fertility with special focus on prostatitis. Hum Reprod Update 1999;5:421–32
La Verda D, Albanese LN, Ruther PE, et al. Seroreactivity to Chlamydia trachomatis Hsp10
correlates with severity of genital tract disease. Infect Immun 2000;68:303–9.
Equils O, Lu D, Gatter M, et al. Chlamydia heat shock protein 60 induces trophoblast
apoptosis through TLR4. J Immunol 2006;177:1257–63.