Ethylene is a plant hormone that regulates many processes in plants including fruit ripening. It is produced through the Yang cycle which converts methionine to ethylene via S-adenosylmethionine and ACC. Ethylene triggers gene expression changes and promotes fruit ripening. Exposing climacteric fruits to low levels of ethylene for at least 12 hours induces ripening. Ethylene treatment is used postharvest for de-greening, sprout inhibition, flower induction, and accelerating fruit ripening. There are different methods for applying ethylene including shot systems, flow-through systems, and using ethylene generators, ethephon, calcium carbide, or enclosure with ripe fruit.