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6/11/2015
1
Ethylene in Postharvest Technology
Part-I
Introduction
• A potent plant growth regulator
• Produced by almost all parts of higher plants, although the rate of
production depends on the type of tissue and the stage of
development.
• Meristematic regions and nodal regions
• Increases during leaf abscission and flower senescence, as well as
during fruit ripening
Role of ethylene
• The concerted expression and/or suppression of these genes
bring about morphological, physiological, and biochemical
changes in plants in diverse events such as seed germination,
abscission, fruit ripening, senescence, and various kinds of
biotic and abiotic stresses.
• It has the ability to evoke several responses in plants by
switching on or off hundreds of genes to affect a process
• mediated by ethylene or other stimuli may evoke response
through ethylene. Therefore, genes that express or suppress in
presence of ethylene may roughly be termed as ethylene-
responsive genes
Changes during fruit ripening: Climacteric fruit
6/11/2015
2
Ethylene Biosynthesis during fruit ripening
• The amino acid methionine is the precursor of ethylene, and ACC (1-
aminocyclopropane-1-carboxylic acid) serves as an intermediate in
the conversion of methionine to ethylene.
• Ethylene production in fruits results from methionine through the
Yang Cycle
• Met AdoMet ACC Ethylene
• Where
• Met = methionine
• AdoMet = S-Adenosyl-methionine
• ACC = 1-aminocyclopropane-1-carboxylic acid
Postharvest Uses of Ethylene
• Flower and sprout induction
• Stimulation of pineapple flowering
• Narcissus bulb treatment with ethylene for a few hours just after lifting
induces almost 100% flowering
• The treatment of ethylene enhances the sproutingof seed potatoes
• Care should be made that this treatment should not prolongs as it leads to
inhibition of their extension growth.
• Shuck loosening and fruit release
• Pre-harvest application of Ethylene releasing chemicals (ethephon) induces
shuck loosening and improves harvest efficiency
• Chlorophyll destruction
• Ethylene treatment results in rapid loss of chlorophyll,the green colour of
leaves and unripe fruit
• De-greening of citrus
• Orange colour is revealed as the chlorophyll is destroyed during ethylene
treatment
• Ethylene treatment is used to accelerate the curing of tobacco
Fruit ripening
• Concentration
• Concentration of ethylene for ripening various commodities vary
• Mostly required in the range of 0.1 to 1 ppm
• Exposure time
• Depends upon on commodity nature
• For climacteric fruit at least 12 hours of exposure time is needed
6/11/2015
3
Technical consideration for fruit ripening
• The effective, uniform and faster ripening depend upon
• Type of fruit
• Maturity stage
• Temperature and RH of ripening room
• Ethylene concentration
• Duration of exposure
• Optimal ripening conditions for fruit
• Temperature 18-25 °C
• RH 90-95%
• Ethylene concentration 10-100 ppm
Ethylene treatment systems
• Several methods are used to provide the proper ethylene
concentration in the ripening room
1- The ‘Shot’ system
• In this system a measured quantities of ethylene are introduced into
ripening room at regular interval
• The shots may be applied by weight or by flow
2-The trickle or Flow-through system
• The ethylene is applied into room continuously rather than
intermittently
• In this system
• To prevent a buildup of CO2
• After every 6 hours, fresh air is drawn
• The air is vented through a small exhaust port
SOURCES OF ETHYLENE
1. Explosion-proof ethylene mixtures
2. Ethylene generators
3. Ethephon
4. Calcium carbide
5. Ripe fruit
6/11/2015
4
1-Explosion-proof ethylene mixtures
• Mixtures of ethylene with inert gas (CO2)
• The proportion of inert gas should be such that at high concentrations
of ethylene
2-Ethylene generators
• A liquid produces ethylene when heated in presence of a catalyst
• The liquid comprises of ethanol and agents
• C2H5OH –H2O C2H4
3- Ethephon
Ethephon (2-chloroethane phosphonic acid)
• Strongly acidic in water
• At above pH 5 hydrolyse to liberate ethylene
• Commercially available form are Ethrel, Florel, Cepa
• Applied as pre-harvest spray
3-CalciumCarbide
• A grayish solid
• When hydrolyzed produces acetylene
• Acetylene contain traces of ethylene
• CaC2 wrapped in newspaper
• 4- Ripe fruit
• Enclosing the unripe fruit with other fruit that are already ripe
• Used in very small scale

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Role of Etylene in Postharvest Technology

  • 1. 6/11/2015 1 Ethylene in Postharvest Technology Part-I Introduction • A potent plant growth regulator • Produced by almost all parts of higher plants, although the rate of production depends on the type of tissue and the stage of development. • Meristematic regions and nodal regions • Increases during leaf abscission and flower senescence, as well as during fruit ripening Role of ethylene • The concerted expression and/or suppression of these genes bring about morphological, physiological, and biochemical changes in plants in diverse events such as seed germination, abscission, fruit ripening, senescence, and various kinds of biotic and abiotic stresses. • It has the ability to evoke several responses in plants by switching on or off hundreds of genes to affect a process • mediated by ethylene or other stimuli may evoke response through ethylene. Therefore, genes that express or suppress in presence of ethylene may roughly be termed as ethylene- responsive genes Changes during fruit ripening: Climacteric fruit
  • 2. 6/11/2015 2 Ethylene Biosynthesis during fruit ripening • The amino acid methionine is the precursor of ethylene, and ACC (1- aminocyclopropane-1-carboxylic acid) serves as an intermediate in the conversion of methionine to ethylene. • Ethylene production in fruits results from methionine through the Yang Cycle • Met AdoMet ACC Ethylene • Where • Met = methionine • AdoMet = S-Adenosyl-methionine • ACC = 1-aminocyclopropane-1-carboxylic acid Postharvest Uses of Ethylene • Flower and sprout induction • Stimulation of pineapple flowering • Narcissus bulb treatment with ethylene for a few hours just after lifting induces almost 100% flowering • The treatment of ethylene enhances the sproutingof seed potatoes • Care should be made that this treatment should not prolongs as it leads to inhibition of their extension growth. • Shuck loosening and fruit release • Pre-harvest application of Ethylene releasing chemicals (ethephon) induces shuck loosening and improves harvest efficiency • Chlorophyll destruction • Ethylene treatment results in rapid loss of chlorophyll,the green colour of leaves and unripe fruit • De-greening of citrus • Orange colour is revealed as the chlorophyll is destroyed during ethylene treatment • Ethylene treatment is used to accelerate the curing of tobacco Fruit ripening • Concentration • Concentration of ethylene for ripening various commodities vary • Mostly required in the range of 0.1 to 1 ppm • Exposure time • Depends upon on commodity nature • For climacteric fruit at least 12 hours of exposure time is needed
  • 3. 6/11/2015 3 Technical consideration for fruit ripening • The effective, uniform and faster ripening depend upon • Type of fruit • Maturity stage • Temperature and RH of ripening room • Ethylene concentration • Duration of exposure • Optimal ripening conditions for fruit • Temperature 18-25 °C • RH 90-95% • Ethylene concentration 10-100 ppm Ethylene treatment systems • Several methods are used to provide the proper ethylene concentration in the ripening room 1- The ‘Shot’ system • In this system a measured quantities of ethylene are introduced into ripening room at regular interval • The shots may be applied by weight or by flow 2-The trickle or Flow-through system • The ethylene is applied into room continuously rather than intermittently • In this system • To prevent a buildup of CO2 • After every 6 hours, fresh air is drawn • The air is vented through a small exhaust port SOURCES OF ETHYLENE 1. Explosion-proof ethylene mixtures 2. Ethylene generators 3. Ethephon 4. Calcium carbide 5. Ripe fruit
  • 4. 6/11/2015 4 1-Explosion-proof ethylene mixtures • Mixtures of ethylene with inert gas (CO2) • The proportion of inert gas should be such that at high concentrations of ethylene 2-Ethylene generators • A liquid produces ethylene when heated in presence of a catalyst • The liquid comprises of ethanol and agents • C2H5OH –H2O C2H4 3- Ethephon Ethephon (2-chloroethane phosphonic acid) • Strongly acidic in water • At above pH 5 hydrolyse to liberate ethylene • Commercially available form are Ethrel, Florel, Cepa • Applied as pre-harvest spray 3-CalciumCarbide • A grayish solid • When hydrolyzed produces acetylene • Acetylene contain traces of ethylene • CaC2 wrapped in newspaper • 4- Ripe fruit • Enclosing the unripe fruit with other fruit that are already ripe • Used in very small scale