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ROLE OF BACTERIA WITH NOVEL
CHARACTERISTICS IN THE PRODUCTION
OF ORGANIC CROP (OKRA)
Mentor: Meenakshi
Mukhopadhyay
Group Members:
1. Piyas Mukherjee (618)
LEADER
2. Shreejit Saha (607) DEPUTY
LEADER
3. Baivabi Bhattacharya (631)
4. Dibyo Mazumdar (645)
WHY ORGANIC FARMING & WHAT IS
PGPR?
WHY ORGANIC FARMING?
1. Maintains optimum soil health. Thus,
the soil capable of supplying all
essential nutrients. This allows crops to
have proper growth and development.
2. This in turn causes the increase in the
productivity and over all improvement of
agro-ecosystem.
3. Organic farming gives quality organic
food and also helps to restore soil
fertility on long term basis.
PLANT GROWTH PROMOTING
RHIZOBACTERIA (PGPR)
1. Improve tolerance to abiotic stress.
2. Colonize the plant root system
3. Compete with other bacteria to
establish themselves. Control
pathogens biologically.
4. Many types and many mechanisms
allow the promotion of the plant
growth.
S3- Bacillus zhanzhouensis strain
MMAM
S7- Bacillus subtilis strain MMAM4
R1- Bacillus subtilis strain MMAM2
ABOUT THE CROP:
Okra (Abelmoschus esculentus)
• Herbaceous, annual plant.
• Malavaceae family.
Best grown: Warm well drained, light sandy to loamy soil.
Okra growing season: Kharif (rainy season) June-July. Can be grown in
January- February with proper watering.
Activity S3 S7 R1
Growth on N-free medium + + + + + +
Growth on Pikovskaya
medium
+ + + + +
Growth on Aleksandro
medium
+ + − −
Amylase Production + ++ + ++ +
Protease Production ++ + ++ + ++ +
HCN Production + + + ++ +
Ammonia Production + + + + +
Catalase Production ++ + ++ + + +
Oxidase Production
Isolated from:
Agricultural land in
Diamond Harbor of
South 24 Parganas, West
Bengal, that has been
used for agricultural
practice for the last 85
years.
Strains: Bacillus
UNDERSTANDING THE PGPR:
SOIL SAMPLE STUDY: 2 TYPES
Gita Nursery
Done on 22.03.21
Patuli Nursery
Done on 22.03.21
SOIL STUDY:
Serial dilution
tubes for
diluting the soil.
Plates prepared by
the process of spread
plating.
10−3 NA
Plate. Spread
Plate
Technique.
10
−3
NA
Plate.
Spread
Plate
Technique
53
colonies.
MATERIALS AND
METHODS:
Soil Preparation:
Majorly 2 types of soil was prepared (1 kg in
total per pot)
1. Simple garden soil (full)
2. Simple garden soil (
3
4
𝑡ℎ) + Vermicompost
(
1
4
𝑡ℎ)
(In the vermicompost, 𝑠𝑜𝑖𝑙: 𝑐𝑜𝑚𝑝𝑜𝑠𝑡 was 1: 1)
Sowing process:
Seed: North East Hybrid Seeds variety
1. Seeds kept in moist cotton cloth for
germination (2nd March).
2. Appearance of sprouts; placed in pots (5-6
per pot), 2-3 cm below the soil (6th March).
(After sowing into soil covered with coco peat.)
Member 1
• Soil (Control)
• Soil + S3 solid
• Soil + S7 solid
• Soil + R1 solid
• Soil + (S3+S7+R1)
solid
• Soil + Vermi (Positive
Control)
• Soil + Vermi + S3 solid
• Soil + Vermi + S7 solid
• Soil + Vermi + R1 solid
• Soil + Vermi + (S3+S7+R1)
solid
Member 2
• Soil (Control)
• Soil + S3 in LB media
• Soil + S7 in LB media
• Soil + R1 in LB media
• Soil + (S3+S7+R1) in LB
media
Member 3
Soil + Vermi (Positive Control)
Soil + Vermi + S3 in LB media
Soil + Vermi + S7 in LB media
Soil + Vermi + R1 in LB media
Soil + Vermi + (S3+S7+R1) in LB
media
*Application of treatment on 10th April (after 5 weeks), 40 ml
COMPOSITION AND TREATMENTS
OF POTS: Consortia:
21 ml of bacterial
culture (7ml each) +
19 ml of water.
Member 4
Soil (Control)
Soil + S3 in Sterile Water
Soil + S7 in Sterile Water
Soil + R1 in Sterile Water
Soil + (S3+S7+R1) in Sterile Water
Member 5
Soil + Vermi (Positive Control)
Soil + Vermi + S3 in Sterile Water
Soil + Vermi + S7 in Sterile Water
Soil + Vermi + R1 in Sterile Water
Soil + Vermi + (S3+S7+R1) in Sterile
Water
*Application of treatment on 16th April (after 6 weeks)
COMPOSITION AND TREATMENTS
OF POTS: Solid pellets:
10000 RPM for 20
mins; supernatant
discarded;
In 40 ml sterile
water.
COMMON MEASURES:
• Watering: twice daily; early morning and evening.
• On around 30th day extra plants were removed (4 each pot).
• Kept under diffused sunlight, direct sunlight and complete shade.
• Data taken every week along with standard errors.
• Both vegetative and reproductive growth were tallied.
WEEK 1: PLANT GROWTH
Dibyo (Member 4)
Saptamita
(Member
5
Piyas (Member 2)
Baivabi
(Member
3)
Shreejit (Member 1)
WEEK 5: PLANT GROWTH
Dibyo (Member 4)
Saptamita
(Member
5
Piyas (Member 2)
Baivabi
(Member
3)
Shreejit (Member 1)
WEEK 10: PLANT GROWTH
Dibyo (Member 4)
Saptamita
(Member
5
Piyas (Member 2)
Baivabi
(Member
3)
Shreejit (Member 1)
RESULTS: VEGETATIVE
PARAMETERS
Germination Rate (in
%)
First Appearance
of Cotyledon
Leaves – 9th
March 2021
Germination Rate –
Seed’s Intrinsic
Factors and
External Physical
Parameters
RESULTS: VEGETATIVE
PARAMETERS
Treatment with
Bacterial Strain –
Increase in Leaf
Number
Steady Phase of
Curve –
Acclimatize to
the New
Environment
RESULTS: VEGETATIVE
PARAMETERS
STEM LENGTH (in cm)
After Inoculation
with Bacterial
Strain – Increase
in Stem Length
PGPR – Induce
Growth
Enhancing
Factors and
Increase in Soil
Nutrient
Content
RESULTS: VEGETATIVE
PARAMETERS
 Vermicompost grown plants – Greater Internode Length
 Plants treated with Water Suspension – Higher Total Photosynthetic
Surface Area
RESULTS: VEGETATIVE
PARAMETERS
RESULTS: REPRODUCTIVE
PARAMETERS
Number and Size of Flower (in
cm)
 First Flowering – 12th April 2021
 Average Number of Flowers per Plant –
4
 Range of Flower Size – 3 cm to 5 cm
 First Bud Appeared – 3rd April 2021
 Average Number of Buds per Plant – 6
 Range of Size of Bud – 0.5 cm to 1.5 cm
Number and Size of Fruit (in
cm)
 First Appearance of Fruit – 15th April
2021
 Average Number of Fruits per Plant – 4
 Range of Fruit Size – 12 cm to 15 cm
 Date of Harvesting Fruit – 8th May 2021
CONCLUSION:
The plants were maintained and observed both before & after the
addition of treatments, till the week 10 mark.
The best growth was seen in:
Member 1: S3 in Solid Treatment, closely followed by S7 (In both the
sets of Pots)
Member 3: R1in LB and S7 in LB
Member 4: S3 in Water Suspension and S7 in Water Suspension
Member 5: S3 in Water Suspension and S7 in Water Suspension
FUTURE PROSPECTS
The future prospects seen due to organic farming are quite direct:
1. It allows the growth of more nutritional food.
2. It maintains and increases of the soil fertility and productivity.
3. Avoidance of pollution.
4. It prevents the degradation of the natural microflora of the soil.
5. It allows one to fetch higher prices in national and international market.
6. It also increases employability.
In the long run it also allows the protection of the ecosystem indirectly. It
also promotes eco-tourism.
Further research are being carried out by scientists to understand the
process better and also improve the technology and methods of organic
faming.
REFERENCES:
Ashrafuzzaman, M., Nuruzzaman, M., Islam, M. Z., & Islam, M. R. (2003). Effect of Biofertilizers on Vegetative Growth
of Okra. Korean Journal of Crop Science, 48.
Sundari, U. & Gandhi, A. (2017). Effect of microbial inoculants on growth and yield of Bhendi [Abelmoschus esculentus
(L.) Moench] in field trial. International Journal of Advanced Research in Biological Sciences (IJARBS), 4(4), 182-188.
https://doi.org/10.22192/ijarbs.2017.04.04.02
Nayak, Bara, et.al. (2019) Effect of organic, inorganic and bio fertilizers on growth, seed yield and quality traits of okra
[Abelmoschus esculentus (L.) Moench]. The Pharma Innovation Journal 2019; 8(7): 468-473
Nuruzzaman, M., Ashrafuzzaman, M., Islam, M. Z., & Islam, M. R. (2003). Field efficiency of biofertilizers on the
growth of okra (Abelmoschus esculentus [(L.) Moench]). Journal of Plant Nutrition and Soil Science, 166(6), 764–770.
https://doi.org/10.1002/jpln.200321195
Mal, B., Mahapatra, P., Mohanty, S., & Mishra, H. N. (n.d.). Growth and yield parameters of okra (Abelmoschus
esculentus) influenced by Diazotrophs and chemical fertilizers. 6.
 agriculturalchemistry. (03:17:54 UTC). Organic farming prospects and constraints. https://www.slideshare.net/agriculturalchemistry/organic-farming-
prospects-and-constraints
agriculturalchemistry. (03:17:54 UTC). Organic farming prospects and constraints.
https://www.slideshare.net/agriculturalchemistry/organic-farming-prospects-and-constraints
Shiba Shankar Pattayat. (19:18:57 UTC). Growth and performance of organic farming in India.
AKNOWLEDGEMENTS
A special thank you to Father Principal, Dr. Arup Kumar Mitra, the
Head of the Department of Microbiology, Professors, our Mentor,
Meenakshi Mukhopadhyay, all the research scholars, Bharat
Foundation and everyone who have helped us prepare this
presentation.
THANK YOU!
Role of bacteria with novel characteristics in the production of Organic Crop (okra)

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Role of bacteria with novel characteristics in the production of Organic Crop (okra)

  • 1.
  • 2. ROLE OF BACTERIA WITH NOVEL CHARACTERISTICS IN THE PRODUCTION OF ORGANIC CROP (OKRA) Mentor: Meenakshi Mukhopadhyay Group Members: 1. Piyas Mukherjee (618) LEADER 2. Shreejit Saha (607) DEPUTY LEADER 3. Baivabi Bhattacharya (631) 4. Dibyo Mazumdar (645)
  • 3. WHY ORGANIC FARMING & WHAT IS PGPR? WHY ORGANIC FARMING? 1. Maintains optimum soil health. Thus, the soil capable of supplying all essential nutrients. This allows crops to have proper growth and development. 2. This in turn causes the increase in the productivity and over all improvement of agro-ecosystem. 3. Organic farming gives quality organic food and also helps to restore soil fertility on long term basis. PLANT GROWTH PROMOTING RHIZOBACTERIA (PGPR) 1. Improve tolerance to abiotic stress. 2. Colonize the plant root system 3. Compete with other bacteria to establish themselves. Control pathogens biologically. 4. Many types and many mechanisms allow the promotion of the plant growth. S3- Bacillus zhanzhouensis strain MMAM S7- Bacillus subtilis strain MMAM4 R1- Bacillus subtilis strain MMAM2
  • 4. ABOUT THE CROP: Okra (Abelmoschus esculentus) • Herbaceous, annual plant. • Malavaceae family. Best grown: Warm well drained, light sandy to loamy soil. Okra growing season: Kharif (rainy season) June-July. Can be grown in January- February with proper watering.
  • 5. Activity S3 S7 R1 Growth on N-free medium + + + + + + Growth on Pikovskaya medium + + + + + Growth on Aleksandro medium + + − − Amylase Production + ++ + ++ + Protease Production ++ + ++ + ++ + HCN Production + + + ++ + Ammonia Production + + + + + Catalase Production ++ + ++ + + + Oxidase Production Isolated from: Agricultural land in Diamond Harbor of South 24 Parganas, West Bengal, that has been used for agricultural practice for the last 85 years. Strains: Bacillus UNDERSTANDING THE PGPR:
  • 6. SOIL SAMPLE STUDY: 2 TYPES Gita Nursery Done on 22.03.21 Patuli Nursery Done on 22.03.21
  • 7. SOIL STUDY: Serial dilution tubes for diluting the soil. Plates prepared by the process of spread plating. 10−3 NA Plate. Spread Plate Technique. 10 −3 NA Plate. Spread Plate Technique 53 colonies.
  • 8. MATERIALS AND METHODS: Soil Preparation: Majorly 2 types of soil was prepared (1 kg in total per pot) 1. Simple garden soil (full) 2. Simple garden soil ( 3 4 𝑡ℎ) + Vermicompost ( 1 4 𝑡ℎ) (In the vermicompost, 𝑠𝑜𝑖𝑙: 𝑐𝑜𝑚𝑝𝑜𝑠𝑡 was 1: 1) Sowing process: Seed: North East Hybrid Seeds variety 1. Seeds kept in moist cotton cloth for germination (2nd March). 2. Appearance of sprouts; placed in pots (5-6 per pot), 2-3 cm below the soil (6th March). (After sowing into soil covered with coco peat.)
  • 9. Member 1 • Soil (Control) • Soil + S3 solid • Soil + S7 solid • Soil + R1 solid • Soil + (S3+S7+R1) solid • Soil + Vermi (Positive Control) • Soil + Vermi + S3 solid • Soil + Vermi + S7 solid • Soil + Vermi + R1 solid • Soil + Vermi + (S3+S7+R1) solid Member 2 • Soil (Control) • Soil + S3 in LB media • Soil + S7 in LB media • Soil + R1 in LB media • Soil + (S3+S7+R1) in LB media Member 3 Soil + Vermi (Positive Control) Soil + Vermi + S3 in LB media Soil + Vermi + S7 in LB media Soil + Vermi + R1 in LB media Soil + Vermi + (S3+S7+R1) in LB media *Application of treatment on 10th April (after 5 weeks), 40 ml COMPOSITION AND TREATMENTS OF POTS: Consortia: 21 ml of bacterial culture (7ml each) + 19 ml of water.
  • 10. Member 4 Soil (Control) Soil + S3 in Sterile Water Soil + S7 in Sterile Water Soil + R1 in Sterile Water Soil + (S3+S7+R1) in Sterile Water Member 5 Soil + Vermi (Positive Control) Soil + Vermi + S3 in Sterile Water Soil + Vermi + S7 in Sterile Water Soil + Vermi + R1 in Sterile Water Soil + Vermi + (S3+S7+R1) in Sterile Water *Application of treatment on 16th April (after 6 weeks) COMPOSITION AND TREATMENTS OF POTS: Solid pellets: 10000 RPM for 20 mins; supernatant discarded; In 40 ml sterile water. COMMON MEASURES: • Watering: twice daily; early morning and evening. • On around 30th day extra plants were removed (4 each pot). • Kept under diffused sunlight, direct sunlight and complete shade. • Data taken every week along with standard errors. • Both vegetative and reproductive growth were tallied.
  • 11. WEEK 1: PLANT GROWTH Dibyo (Member 4) Saptamita (Member 5 Piyas (Member 2) Baivabi (Member 3) Shreejit (Member 1)
  • 12. WEEK 5: PLANT GROWTH Dibyo (Member 4) Saptamita (Member 5 Piyas (Member 2) Baivabi (Member 3) Shreejit (Member 1)
  • 13. WEEK 10: PLANT GROWTH Dibyo (Member 4) Saptamita (Member 5 Piyas (Member 2) Baivabi (Member 3) Shreejit (Member 1)
  • 14. RESULTS: VEGETATIVE PARAMETERS Germination Rate (in %) First Appearance of Cotyledon Leaves – 9th March 2021 Germination Rate – Seed’s Intrinsic Factors and External Physical Parameters
  • 15.
  • 16.
  • 17.
  • 18.
  • 19.
  • 20. RESULTS: VEGETATIVE PARAMETERS Treatment with Bacterial Strain – Increase in Leaf Number Steady Phase of Curve – Acclimatize to the New Environment
  • 22. After Inoculation with Bacterial Strain – Increase in Stem Length PGPR – Induce Growth Enhancing Factors and Increase in Soil Nutrient Content RESULTS: VEGETATIVE PARAMETERS
  • 23.  Vermicompost grown plants – Greater Internode Length  Plants treated with Water Suspension – Higher Total Photosynthetic Surface Area RESULTS: VEGETATIVE PARAMETERS
  • 24. RESULTS: REPRODUCTIVE PARAMETERS Number and Size of Flower (in cm)  First Flowering – 12th April 2021  Average Number of Flowers per Plant – 4  Range of Flower Size – 3 cm to 5 cm  First Bud Appeared – 3rd April 2021  Average Number of Buds per Plant – 6  Range of Size of Bud – 0.5 cm to 1.5 cm Number and Size of Fruit (in cm)  First Appearance of Fruit – 15th April 2021  Average Number of Fruits per Plant – 4  Range of Fruit Size – 12 cm to 15 cm  Date of Harvesting Fruit – 8th May 2021
  • 25. CONCLUSION: The plants were maintained and observed both before & after the addition of treatments, till the week 10 mark. The best growth was seen in: Member 1: S3 in Solid Treatment, closely followed by S7 (In both the sets of Pots) Member 3: R1in LB and S7 in LB Member 4: S3 in Water Suspension and S7 in Water Suspension Member 5: S3 in Water Suspension and S7 in Water Suspension
  • 26. FUTURE PROSPECTS The future prospects seen due to organic farming are quite direct: 1. It allows the growth of more nutritional food. 2. It maintains and increases of the soil fertility and productivity. 3. Avoidance of pollution. 4. It prevents the degradation of the natural microflora of the soil. 5. It allows one to fetch higher prices in national and international market. 6. It also increases employability. In the long run it also allows the protection of the ecosystem indirectly. It also promotes eco-tourism. Further research are being carried out by scientists to understand the process better and also improve the technology and methods of organic faming.
  • 27. REFERENCES: Ashrafuzzaman, M., Nuruzzaman, M., Islam, M. Z., & Islam, M. R. (2003). Effect of Biofertilizers on Vegetative Growth of Okra. Korean Journal of Crop Science, 48. Sundari, U. & Gandhi, A. (2017). Effect of microbial inoculants on growth and yield of Bhendi [Abelmoschus esculentus (L.) Moench] in field trial. International Journal of Advanced Research in Biological Sciences (IJARBS), 4(4), 182-188. https://doi.org/10.22192/ijarbs.2017.04.04.02 Nayak, Bara, et.al. (2019) Effect of organic, inorganic and bio fertilizers on growth, seed yield and quality traits of okra [Abelmoschus esculentus (L.) Moench]. The Pharma Innovation Journal 2019; 8(7): 468-473 Nuruzzaman, M., Ashrafuzzaman, M., Islam, M. Z., & Islam, M. R. (2003). Field efficiency of biofertilizers on the growth of okra (Abelmoschus esculentus [(L.) Moench]). Journal of Plant Nutrition and Soil Science, 166(6), 764–770. https://doi.org/10.1002/jpln.200321195 Mal, B., Mahapatra, P., Mohanty, S., & Mishra, H. N. (n.d.). Growth and yield parameters of okra (Abelmoschus esculentus) influenced by Diazotrophs and chemical fertilizers. 6.  agriculturalchemistry. (03:17:54 UTC). Organic farming prospects and constraints. https://www.slideshare.net/agriculturalchemistry/organic-farming- prospects-and-constraints agriculturalchemistry. (03:17:54 UTC). Organic farming prospects and constraints. https://www.slideshare.net/agriculturalchemistry/organic-farming-prospects-and-constraints Shiba Shankar Pattayat. (19:18:57 UTC). Growth and performance of organic farming in India.
  • 28. AKNOWLEDGEMENTS A special thank you to Father Principal, Dr. Arup Kumar Mitra, the Head of the Department of Microbiology, Professors, our Mentor, Meenakshi Mukhopadhyay, all the research scholars, Bharat Foundation and everyone who have helped us prepare this presentation.