Sperm Count Techniques

1. Rapid Test for Semen
Analysis:
Sperm Count

2. What is Semen Analysis?
A semen analysis, also called a sperm count, measures the
quantity and quality of semen and sperm.
Semen is the thick, white fluid released from the penis
during sexual climax (orgasm). This release is called
ejaculation. Semen contains sperm, the cells made in the
male reproductive system that include the genetic material
needed to make a baby.
If you and your partner haven't been able to get pregnant,
a problem with semen or sperm may be one of the reasons.
Problems such as a low sperm count or sperm that don't
move properly can cause infertility, which is the inability
to conceive a baby after a year of trying.

3. A semen analysis can help figure out if a problem with
semen or sperm is likely to be causing infertility.
Other names: sperm count, sperm analysis, semen testing,
male fertility test
The parameters tested in semen analysis includes:
Volume is the amount of semen in your sample. A low
volume may cause infertility.
Sperm count is the number of sperm in the entire
sample. A low count may make it difficult to get your
partner pregnant.
Sperm concentration measures how close together the
sperm are in your semen. A low concentration may be a
sign of a problem.

4. Sperm movement (motility) tells you the percentage of
sperm that are moving forward. Sperm must be able to
move forward to fertilize an egg.
Sperm shape (morphology) is the number of sperm that
have a normal shape. Sperm with abnormal shapes may
not be able to fertilize an egg.
pH describes the acidity of your semen. Abnormal acidity
can kill sperm or affect their ability to move.
White blood cells (leukocytes) in sperm may be a sign
that an infection is affecting your fertility.

5. Procedure in Semen Analysis
1.Collect the semen sample in clean and sterilized plastic
container.
2.To check sperm count dilute the semen sample with
sterilized water.
3.Place a coverslip on the hemocytometer after wetting
supports (rails) with water. This helps to hold down the
coverslip while loading the sperm.
4. Place 10-15 µl of the diluted sperm under the cover slip
on each side of the hemocytometer.
5.Observe the hemocytometer under the microscope.Count
no.of sperms in large 5 square and calculate the sperm
count by formula.
Concentration/ml = (Dilution Factor)(Count in 5
squares)(0.05 X 106)

9. To check vitality of sperms,sperms are stained with
Eosin or Nigrosin.Vital sperms remain colourless and
non vital get stained by the stain.