Your boss has instructed you to make up a medium for growing Gram-negative prokaryotes. She wants you to be able to grow 100 mg/l of volatile suspended solids. You looked up some papers on the microorganisms and found that the empirical chemical formula for them is C_5H_7O_2NP_0.1. What are the minimum concentrations of NH^+_4 -N and PO_4-P that you must add to your medium? Solution Phosphorus and Nitrate were found at a ratio of 1:10 in microbes as studied in the example. So 10+1=11 parts weighs 100 mg, i.e. 100/11=9.090 mg PO4-P and rest 90.909 mg NH4+-N in 1 litre is suggested for your medium..