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Protein-RNA complexes, ALS and aging
Jernej Ule, ulelab.info
iCLIP: nucleotide-resolution crosslinking and immunoprecipitation
Konig et al., NSMB, 2010
Haberman et al, Gen biol, 2017
Tollervey et al., Nat Neuro, 2011
data at http://icount.biolab.si
TDP-43 regulates splicing via UG repeat sequences
regulated exon
Splicing of TDP-43-regulated alternative exons in human brain
Tollervey et al., Genome Res, 2013
disease-specific
aging-associated
Alternative splicing affected by aging and/or disease in human brain
Tollervey et al., Genome Res, 2013
disease-specific
aging-associated
Analysis of cell-type specific gene expression
Tollervey et al., Genome Res, 2013
Soreq et al., Cell Rep, 2017
Analysis of cell-type specific gene expression upon aging
Soreq et al., Cell Rep, 2017
Shifts in regional expression profiles of glia-specific genes upon aging
Summary
Analysis of splicing changes revealed a common component of aging and neurodegeneration
Dramatic decrease of neuron-specific genes is a hallmark of neurodegeneration (as expected)
Distinct changes in cell type-specific expression profiles are a hallmark of aging
Microglia-specific genes increase, and neuron-specific genes slightly decrease their expression
Astrocyte and oligodendrocyte-specific genes shift their regional expression patterns
Cell-type specific gene patterns in aging
Mina Ryten, John Hardy,
UK Brain Expression Consortium
James Rose, Colin Smith,
University of Edinburg
Lilach
Soreq
Rickie
Patani
Chris Shaw
KCL, London
James Tollervey
Aging & TDP-43 in ALS

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Protein-RNA Complexes, ALS and Aging

  • 1. Protein-RNA complexes, ALS and aging Jernej Ule, ulelab.info
  • 2. iCLIP: nucleotide-resolution crosslinking and immunoprecipitation Konig et al., NSMB, 2010 Haberman et al, Gen biol, 2017
  • 3. Tollervey et al., Nat Neuro, 2011 data at http://icount.biolab.si TDP-43 regulates splicing via UG repeat sequences regulated exon
  • 4. Splicing of TDP-43-regulated alternative exons in human brain Tollervey et al., Genome Res, 2013 disease-specific aging-associated
  • 5. Alternative splicing affected by aging and/or disease in human brain Tollervey et al., Genome Res, 2013 disease-specific aging-associated
  • 6. Analysis of cell-type specific gene expression Tollervey et al., Genome Res, 2013
  • 7. Soreq et al., Cell Rep, 2017 Analysis of cell-type specific gene expression upon aging
  • 8. Soreq et al., Cell Rep, 2017 Shifts in regional expression profiles of glia-specific genes upon aging
  • 9. Summary Analysis of splicing changes revealed a common component of aging and neurodegeneration Dramatic decrease of neuron-specific genes is a hallmark of neurodegeneration (as expected) Distinct changes in cell type-specific expression profiles are a hallmark of aging Microglia-specific genes increase, and neuron-specific genes slightly decrease their expression Astrocyte and oligodendrocyte-specific genes shift their regional expression patterns
  • 10. Cell-type specific gene patterns in aging Mina Ryten, John Hardy, UK Brain Expression Consortium James Rose, Colin Smith, University of Edinburg Lilach Soreq Rickie Patani Chris Shaw KCL, London James Tollervey Aging & TDP-43 in ALS