Protein Digestion Experiment in Biochemistry

2. ~They are biochemical compounds consisting of one or
more polypeptides typically folded into a
globular or fibrous form, facilitating a biological function.
~They are made up of Amino Acids.

3. The process of digestion is defined as the ‘process by
which macromolecules in food are broken down into
their component small-molecule subunits’.
The macromolecules are the proteins or polypeptides
themselves, and the subunits are the amino acids. The
bonds holding the subunits together are peptide bonds

4. is the degradation of proteins by cellular enzymes enzymes
in a process called hydrolysis.
Protein digestion takes place in two different
phases:
~~In the stomach
~~In the small intestine

6. Contents Results Action
Test Tube 1
Egg Albumin/ Grated Egg
White + 1mL Pepsin Sol’n
Purple Partial Digestion
Test Tube 2
Egg Albumin/ Grated Egg
White + 1mL Pepsin Sol’n +
2 drops HCL
Pinkish Lavender
With Hydrolysis /
Complete
Digestion
Test Tube 3 Egg Albumin/ Grated Egg Purple No Digestion

7. Contents Results Action
Test Tube 1
Egg Albumin/ Grated Egg
White + 1mL 5% Trypsin
Purple Partial Digestion
Test Tube 2
Egg Albumin/ Grated Egg
White + 1mL 5% Trypsin +
2mL 0.5% Na2CO3
Pinkish Lavender /
Blue
With Hydrolysis
/ Complete
Digestion
Test Tube 3
Egg Albumin/ Grated Egg
White + 2mL 0.5% Na2CO3
Purple
Partial
Digestion

8. BIURET Test is a chemical test used for detecting presence of the
peptide bonds.
(+) = Purple / Violet
HYDROLYSIS is a reaction involving the breaking of a bond in a
molecule using water. The reaction mainly occurs between an ion
and water molecules and often changes the pH of a solution.
Protein hydrolysis is the breakdown of protein into smaller
peptides and free amino acids. Specialty Enzymes has animal-
based proteases like trypsin, plant based proteases like papain
and a large variety of microbial source proteases.