Presentation Undergrad Reaserch Day
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This study examines using electron beam lithography patterned surfaces and DNA origami structures to enhance metal fluorescence. Gold nanoparticles of different sizes and spacings were created on silicon wafers using electron beam lithography. Fluorescence was measured in the presence of these gold nanoparticles and quantum dots. A second approach used DNA origami to control the spacing and distance of gold nanoparticles from quantum dots, synthesizing a 3x3 cross-shaped DNA origami structure with gold nanoparticles and quantum dots attached at specific staple strands. The goal is to increase fluorescence for applications in biomedical imaging and other fields.
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Porosity and the Magnetic Properties of Aluminium Doped Nickel Ferrite
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This document provides an overview of various techniques used in biotechnology and molecular biology to study DNA, including X-ray crystallography, chromatography, electrophoresis, ultracentrifugation, UV spectroscopy, restriction enzymes, DNA cloning, sequencing, probes, Southern blotting, and polymerase chain reaction. These techniques have contributed to understanding the structure of DNA and sequencing the human genome, enabling research on genetic diseases.
Nanotechnology parasitology 20111112
The document summarizes the history and development of nanotechnology. It discusses how the concept was first developed by Richard Feynman in 1959, and the term was coined by Norio Taniguchi in 1974. It then outlines key milestones and advancements in the 1980s and beyond that helped establish nanotechnology as a field, including the invention of the scanning tunneling microscope in 1981 and discoveries of fullerenes in 1985 and carbon nanotubes. The document also provides examples of how nanotechnology is being applied in biology and medicine, such as using atomic force microscopes to image cells, optical tweezers to manipulate organisms, and quantum dots for labeling parasites.
Ion beam irradiation of polycarbonate_TEST
Ion beam irradiation was used to study the effects on polycarbonate polymers. Ion beams can excite atoms and molecules, generate phonons and heat samples. This can lead to breaking of chemical bonds, ionizations, and formation of radicals and defects. Radicals generated by ion beams can cause macromolecular chain scission or crosslinking. Spectroscopic analysis using ESR, UV-VIS and luminescence revealed that ion beam irradiation generates free radicals along particle tracks coupled by exchange interactions. The concentration of free radicals and deposited dose depends on penetration depth and linear energy transfer.
Current and future techniques for cancer diagnosis
This document discusses various nanotechnology approaches for cancer diagnosis, including the use of gold nanoparticles, quantum dots, carbon nanotubes, and nanoflares. Gold nanoparticles can be used for detection through techniques like dynamic light scattering and surface plasmon coupling. Quantum dots and carbon nanotubes can also be functionalized for ultrasensitive detection of cancer biomarkers. Emerging tools like nanoflares allow for detection of genetic targets associated with cancer within living cells. Overall, nanotechnology enables low detection limits and early cancer diagnosis.
CHM1051L_Poster_2014.doc
This document discusses the synthesis and analysis of cadmium selenide (CdSe) quantum dots. CdSe nanoparticles were synthesized using a three-neck flask to heat precursors in an inert atmosphere. Two samples with different fluorescence wavelengths were produced - one at 566 nm and one at 610 nm. Single molecule spectroscopy was used to study the blinking behavior of individual quantum dots. While the samples produced were too dim to fully analyze blinking, the research provided insight into energy transfer processes in nanoscale systems with applications in lighting, imaging, and other technologies.
Nanofiber Research Paper
This lab report summarizes experiments conducted on pristine CuO nanoparticles and Na-doped CuO nanoparticles. Transmission electron microscopy images showed that the pristine CuO nanoparticles had a leaf-like flake morphology with sizes between 500-600nm thick. Fourier transform infrared spectroscopy confirmed the presence of CuO and Na2O peaks and showed red and blue shifts with Na doping. UV-Vis spectroscopy showed that the band gap decreased from 1.49eV to 1.46eV with low Na doping but increased above 1.5eV with higher doping levels due to secondary phase formation. Photoluminescence spectroscopy revealed defect energy levels within the band gap.
X-RAY CRYSTALLOGRAPHY.pptx
X-ray Crystallography is a scientific method used to determine the arrangement of atoms of a crystalline solid in three dimension. It is based on x ray diffraction. Reveals structure of a crystal at atomic level.
46 Complete Photoproduction Experiments - AIP Conference Proceedings, October...
46 Complete Photoproduction Experiments - AIP Conference Proceedings, October...Cristian Randieri PhD
Complete Photoproduction Experiments - 12th International Conference on Meson-Nucleon Physics and the Structure of the Nucleon, Virginia, USA, 31 May-4 June 2010. AIP Conference Proceedings, October 2011, Vol. 1374, pp. 17-22, ISSN: 0094-243X, doi: 10.1063/1.3647092
di A. D’Angelo, K. Ardashev, C. Bade, O. Bartalini, V. Bellini, M. Blecher, J. P. Bocquet, M. Capogni, A. Caracappa, L. E. Casano, M. Castoldi, R. Di Salvo, A. Fantini, D. Franco, G. Gervino, F. Ghio, G. Giardina, C. Gibson, B. Girolami, A. Giusa, H. Glu, K. Hicks, S. Hoblit, A. Honig, T. Kageya, M. Khandaker, O. C. Kistner, S. Kizilgul, S. Kucuker, A. Lapikf, A. Lehmann, P. Levi Sandri, A. Lleres, M. Lowry, M. Lucas, J. Mahon, F. Mammoliti, G. Mandaglio, M. Manganaro, L. Miceli, D. Moricciani, A. Mushkarenkovf, V. Nedorezovf, B. Norum, M. Papb, B. Preedom, H. Seyfarthb, C. Randieri, D. Rebreyend, N. Rudnevf, G. Russo, A. Sandorfi, C. Schaerf, M. L. Sperduto, H. Stroher, M. C. Sutera, C. E. Thorn, A. Turingef, V. Vegna, C. S. Whisnanth, K. Wang, X. Wei (2011)
Abstract
The extraction of resonance parameters from meson photo-reaction data is a challenging effort, that would greatly benefit from the availability of several polarization observables, measured for each reaction channel on both proton and neutron targets. In the aim of obtaining such complete experiments, polarized photon beams and targets have been developed at facilities, worldwide. We report on the latest results from the LEGS and GRAAL collaborations, providing single and double polarization measurements on pseudo-scalar meson photo-production from the nucleon.DNA Chip
DNA chips, also known as microarrays, contain thousands of genes arranged on a small chip. Each gene occupies its own spot on the chip. By introducing genetic samples onto the chip, researchers can determine which genes are most active and gain insights into how genetics affect traits and diseases. Emerging nanotechnologies allow the creation of even smaller DNA chips with higher resolution using techniques like dip pen nanolithography and electrochemical sensing. These nano-scale DNA chips have the potential to revolutionize the study of genomics and functional genomics.
Nle sure poster
This document summarizes a study that investigated how the size and spacing of gold nanoparticles affect the enhancement of quantum dot fluorescence. Quantum dots placed near gold nanoparticles experience metal-enhanced fluorescence, where the nanoparticles act as antennas to increase the dots' fluorescent intensity. The study created silicon wafers with varying sizes (50-70nm) and spacings (6-7x) of gold nanoparticles. Quantum dots were successfully attached to the gold using a thiol-polyethylene glycol-biotin linker. Results showed that 50nm and 70nm gold nanoparticles with a 7x or 6x spacing provided the highest fluorescence enhancement.
DNA sequencing by kk sahu sir
INTRODUCTION
DEFINITION
HISTORY
METHODS OF DNA SEQUENCING
MAXAM GILBERT METHOD
SANGERS METHOD
AUTOMATED DNA SEQUENCER
PYROSEQUENCING
SHOTGUN SEQUENCING
DNA MICROARRAY
APPLICATION
CONCLUSION
REFRENCES
Similar to Presentation Undergrad Reaserch Day (20)
Single Molecule Sequence Detection Via Microfluidic Planar Extensional Flow
Single Molecule Sequence Detection Via Microfluidic Planar Extensional Flow
Soft x-ray nanoanalytical tools for thin film organic electronics
Soft x-ray nanoanalytical tools for thin film organic electronics
Current and future techniques for cancer diagnosis
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46 Complete Photoproduction Experiments - AIP Conference Proceedings, October...
46 Complete Photoproduction Experiments - AIP Conference Proceedings, October...
Presentation Undergrad Reaserch Day
- 1. Study of metal fluorescence enhancement using electron beam lithography patterned surfaces and DNA Origami structures Nguyet Le, Dr. Timothy Corrigan and Ina Nikolli Concord University, Division of Natural Sciences, Athens WV 24712 David Neff and Dr. Michael Norton. Marshall University, Chemistry Department, Huntington WV 25701 Do Not Write Below This Line (4) Fluorescence occurs when a molecule or substance absorbs light at one wavelength (color) and then re-emits light at a longer wavelength (different color). Used in several biomedical sciences: - Imaging cells - Determine protein and DNA structures - Examining antibodies for developing pharmaceuticals Introduction Acknowledgements Thanks to West Virginia Trust Fund to fund this research. Thank to SURE program of Marshall University for funding this research. References 1.T.D. Corrigan, S.-H. Gou, R. J. Phaneuf, and H. Szmacinski, Appl. Phys. Lett. 88, 101112 (2006). 2. T.D. Corrigan, S.-H. Gou, R. J. Phaneuf, and H. Szmacinski, J. fluoresc. 15, 777-784 (2005). 3. Quantum Dots. Pharmainfo.net.2007. April 8th, 2011. http://www.pharmainfo.net/reviews/quantum-dots-novel- technique-drug-delivery-and-therapy. 4. Carsten Sonnichsen, Plasmons in metal nanostructures. June 20th 2001 http://edoc.ub.uni- muenchen.de/2367/1/Soennichsen_Carsten.pdf77 (2005). First approach: Silicon wafer with different size and spacing of gold nanoparticles was created using e-beam lithography. The chemistry and data results are shown below. Experimental Procedure ResultsQuantum dots are tiny semiconductor nanocrystals that give off fluorescence when they are stimulated by light. The purpose of this research is to increase fluorescence using these quantum dots in the presence of gold nanoparticles using beam lithography patterned surfaces. A second approach taken using DNA Origami as structures to control spacing and distance of the Au particles with the quantum dots. Single stranded DNA has the ability to recognize complementary single DNA strands using Watson-Crick base pairing, to which QD are added. Second Approach: 3x3 cross shaped DNA Origami nanopattern was synthesized using approximately 200 short sample DNA structures which are further to attached to AgNP and QD. 38 nm (remeasure) 30 nm AuNPs ~16.3 nm ~16.3 nm ~64.4 nm center to center, 34.4 nm edge to edge 30nm AuNPs on one dimensional cross origami - AuNPs located at CO3970 staples 99/104 and 163/168 ~100 nm ~64.4nm ~72nm ~136nm ~210nm 5’-……staple……….TAATAATAATAATAATAATAATAAAAAACGCCAAGCTTGCAT-3’ ss42m new 2014 monothiol 5’- /5ThioMC6-D/ATGC AAGCTT GGCG TTTT TTA TTA TTA TTA TTA TTA TTA TTA-3’ DNA Origami on a 1X1 crossed shape has been synthesized using (ingredients). The synthesis of DNA Origami structures were confirmed using the AFM. The next step includes the synthesis of 3x3 crossed shaped DNA origami structures in collaboration will Marshall University . Following picture shows the entire DNA Sequence and the particular “stample strands’ used to hold the scaffold in place. Considering that the bases as constrained by design and period of dna helix, aptamers here project 'up' out of the plane of the origami arms as shown colored rings which simply indicate approximate tether staple positions ~5.5 nm ~5.5 nm a ~25nm- calibrated in X Rothemund/Neff/Hong UP out of page positions TBA (this indicates that the positions of TBAs out of plane of co3970 were checked against the Rothemund work and checked with Hong) CO-3970each base pair =.34nm