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Whose articles cite a body of work? Is this a high-impact journal? How might others assess my scholarly impact? Citation analysis is one of the primary methods used to answer these questions.
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An introduction in the world of Social Network Analysis and a view on how this may help learning networks. History, data collection and several analysis techniques are shown.
A high-level overview of social network analysis using gephi with your exported Facebook friends network. See more network analysis at http://allthingsgraphed.com.
Quick introduction to community detection.
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For the full video of this presentation, please visit:
https://www.embedded-vision.com/platinum-members/bdti/embedded-vision-training/videos/pages/may-2017-embedded-vision-summit
For more information about embedded vision, please visit:
http://www.embedded-vision.com
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Different Algorithms used in classification [Auto-saved].pptxAzad988896
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NIT Silchar ML Hackathon 2019 Session on Computer Vision with Deep Learning.
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Introduction:
RNA interference (RNAi) or Post-Transcriptional Gene Silencing (PTGS) is an important biological process for modulating eukaryotic gene expression.
It is highly conserved process of posttranscriptional gene silencing by which double stranded RNA (dsRNA) causes sequence-specific degradation of mRNA sequences.
dsRNA-induced gene silencing (RNAi) is reported in a wide range of eukaryotes ranging from worms, insects, mammals and plants.
This process mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, and regulates the expression of protein-coding genes.
What are small ncRNAs?
micro RNA (miRNA)
short interfering RNA (siRNA)
Properties of small non-coding RNA:
Involved in silencing mRNA transcripts.
Called “small” because they are usually only about 21-24 nucleotides long.
Synthesized by first cutting up longer precursor sequences (like the 61nt one that Lee discovered).
Silence an mRNA by base pairing with some sequence on the mRNA.
Discovery of siRNA?
The first small RNA:
In 1993 Rosalind Lee (Victor Ambros lab) was studying a non- coding gene in C. elegans, lin-4, that was involved in silencing of another gene, lin-14, at the appropriate time in the
development of the worm C. elegans.
Two small transcripts of lin-4 (22nt and 61nt) were found to be complementary to a sequence in the 3' UTR of lin-14.
Because lin-4 encoded no protein, she deduced that it must be these transcripts that are causing the silencing by RNA-RNA interactions.
Types of RNAi ( non coding RNA)
MiRNA
Length (23-25 nt)
Trans acting
Binds with target MRNA in mismatch
Translation inhibition
Si RNA
Length 21 nt.
Cis acting
Bind with target Mrna in perfect complementary sequence
Piwi-RNA
Length ; 25 to 36 nt.
Expressed in Germ Cells
Regulates trnasposomes activity
MECHANISM OF RNAI:
First the double-stranded RNA teams up with a protein complex named Dicer, which cuts the long RNA into short pieces.
Then another protein complex called RISC (RNA-induced silencing complex) discards one of the two RNA strands.
The RISC-docked, single-stranded RNA then pairs with the homologous mRNA and destroys it.
THE RISC COMPLEX:
RISC is large(>500kD) RNA multi- protein Binding complex which triggers MRNA degradation in response to MRNA
Unwinding of double stranded Si RNA by ATP independent Helicase
Active component of RISC is Ago proteins( ENDONUCLEASE) which cleave target MRNA.
DICER: endonuclease (RNase Family III)
Argonaute: Central Component of the RNA-Induced Silencing Complex (RISC)
One strand of the dsRNA produced by Dicer is retained in the RISC complex in association with Argonaute
ARGONAUTE PROTEIN :
1.PAZ(PIWI/Argonaute/ Zwille)- Recognition of target MRNA
2.PIWI (p-element induced wimpy Testis)- breaks Phosphodiester bond of mRNA.)RNAse H activity.
MiRNA:
The Double-stranded RNAs are naturally produced in eukaryotic cells during development, and they have a key role in regulating gene expression .
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Spectroscopy is a branch of science dealing the study of interaction of electromagnetic radiation with matter.
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Exploratory Social Network Analysis with Pajek: Attributes & Relations
1.
2. PROPERTY
• Relational: Measure of Relations between 2 Nodes
• Line Value
• Non-Relational: Measure of Relation’s Parties
• Vertex Value: Color, Size, …
SVG Export
3. NON-RELATIONAL PROPERTY
• Discrete
Domain
• Continuous Number
Vector Partition
Structura
l
Attribute
• Statistical & Known Beforehand • Study of Network
• Structural Vector: Coordination of Vertex in an
Image
• Structural Partition: Central or Joining Vertex
• Partition Attribute: Poor or Wealthy Vertex
• Vector Attribute: ?
4. PARTITION PROPERTY
SMALL Discrete Value Domain Set
Tries to partition the vertices into classes of same value
Examples
Gender = {Male, Female}; Partition Attribute
Density = {Low, High, Huge}; Structural Partition
Partition values for each vertex is stored in separate *.clu file. The values can be edited.
By selecting a partition, it can drawn by Draw > Network + First Partition
5. VECTOR PROPERTY
Continuous Infinite Value Domain Set
• Example
• Coordination of Vertices in 2D (x, y) in R2 ; Structural Vector
• In case Vertices Represent Human Height, Weight, .. ; Vector Attribute
• Partition Vector
• Just Proper Meaning is Needed
• Vector Partition
• Categorization of Values;
• Less, Between, Greater; e.g. Height Tall, Medium, Short
• Truncate the Absolute Value
Vector values for each vertex is stored in separate *.vec file. The values can be edited.
By selecting a vector, it can drawn by Draw > Network + First Vector
7. REDUCTION SUBNETWORK
In Case the Network is Very Large or Complex, Concentrate in Portion or
Generalize all of it …
Partition Property is the Main Tool
• Local View (Zoom In): Just Vertices of Same Partition Value
• Global View (Zoom Out): Treat Vertices of Same Partition Value as ONE
Vertex
• Contextual View: One Group to Zoom IN the Others to Zoom Out
• Exceptional Global View
8. Local View of North American Countries
Contextual View of Asian
Countries toward Other Continents
Global View of Countries as Continents
9. SUBNETWORK
• Extract Second Partition from First in Local View
• The Subnetwork Looses its Connection to the Original Network Partitions!
• Solution
1. Network has Two Partition P1 & P2
2. Extract Subnetwork for Value X of Partition P1
3. Want to Know P2 Values for the Subnetwork
4. Extract P1 for the Value X from P2 New Partition is Created
• Extract Subvector in Local View
• Accompany Local View based on a Partition with Vector
• Shrink Vector in Global View
• Accompany Global View based on a Partition with Vector
• For Each Partition of Same Value for Vertices Which Vector Value is
Chosen?
• Min, Max, Mean, …
10. TEMPORAL ANALYSIS
A Vertex may Change its Value of a Partition Value Migration to another
Value
• Statistical Analysis of the Change
• Cross-Tab
• Associativity
• Needs Two Partition File for the Same Partition Property for Different Time
11. QUESTION
• Reduction by Vector Extract Subnetwork by Vector Values
• Solution: