This document discusses a study that investigated the effects of consuming ginger through diet on antioxidant status in rats. The following key points were reported:
- Rats were fed diets with 0%, 0.5%, 1%, or 5% ginger powder for 1 month.
- Ginger significantly increased the levels of antioxidant enzymes (SOD, catalase, GSHPx) in rat livers.
- Lipid peroxidation and protein oxidation were significantly reduced in rat livers and kidneys of ginger-fed groups, indicating ginger's antioxidant effects.
- The findings suggest that regular ginger intake through diet can protect tissues against oxidative damage.
Hepatoprotective activity of aqueous extract of Hibiscus Sabdariffa on alcoho...Bhavana Gundavarapu
The aim of present study was to investigate the Hepatoprotective activity of aqueous extract of Hibiscus sabdariffa (Malvaceace) leaves in albino rats on alcohol induced hepatotoxic activity. .
Does allicin combined with vitamin B-complex have superior potentials than al...Prof. Hesham N. Mustafa
BACKGROUND:
The current article aims to explore the protective potentials of α-tocopherol alone and the combination of allicin and vitamin B-complex against lead-acetate neurotoxicity on the cerebellar cortex.
MATERIALS AND METHODS:
Forty rats were divided into four groups (n=10). Group 1 was the control group. Group 2 received 10 mg/kg body weight (BW) of lead acetate. Group 3 was exposed to 10 mg/kg BW of lead acetate plus a combination of allicin (100 mg/kg BW) and vit. B-complex (40 mg/kg BW). Group 4 was administered lead acetate (10 mg/kg BW) and α-tocopherol (100 mg/kg BW). The animals received treatment for sixty days by oral gavage. All the groups were studied ultrastructurally and immunohistochemically with glial fibrillary acidic protein (GFAP).
RESULTS:
The affected groups revealed shrunken and degenerated Purkinje cells with irregular nuclei. The cytoplasm comprised several lysosomes, unhealthy mitochondria, and dilated Golgi saccules. The myelinated nerve fibers demonstrated breaking of the myelin sheaths, apparent vacuoles, and broad axonal spaces. Immunohistochemically, there was a tremendous surge in GFAP-positive astrocytes in the lead acetate-treated group. These histological and ultrastructural variations were ameliorated by the administration of α-tocopherol and the combination of allicin and vit. B complex. Moreover, an apparent decrease in the number of GFAP-positive astrocytes was obvious in the protected groups.
CONCLUSIONS:
Although both α-tocopherol and the combination of allicin and vit. B-complex can be used as possible adjuvant therapies to ameliorate nervous system ailments attributable to lead acetate, α-tocopherol showed more protective potential.
KEYWORDS:
Allicin; Astrocytes; GFAP; Myelin Figure; Oligodendrocyte; Purkinje cells
Does allicin combined with vitamin B-complex have superior potentials than α-...Prof. Hesham N. Mustafa
Background: The current article aims to explore the protective potentials of α-tocopherol
alone and the combination of allicin and vitamin B-complex against lead-acetate
neurotoxicity on the cerebellar cortex.
Materials and methods: Forty rats were divided into four groups (n=10). Group 1 was the
control group. Group 2 received 10 mg/kg body weight (BW) of lead acetate. Group 3 was
exposed to 10 mg/kg BW of lead acetate plus a combination of allicin (100 mg/kg BW)
and vit. B-complex (40 mg/kg BW). Group 4 was administered lead acetate (10 mg/kg
BW) and α-tocopherol (100 mg/kg BW). The animals received treatment for sixty days by
oral gavage. All the groups were studied ultrastructurally and immunohistochemically with
glial fibrillary acidic protein (GFAP).
Results: The affected groups revealed shrunken and degenerated Purkinje cells with
irregular nuclei. The cytoplasm comprised several lysosomes, unhealthy mitochondria, and
dilated Golgi saccules. The myelinated nerve fibers demonstrated breaking of the myelin
sheaths, apparent vacuoles, and broad axonal spaces. Immunohistochemically, there was a
tremendous surge in GFAP-positive astrocytes in the lead acetate-treated group. These
histological and ultrastructural variations were ameliorated by the administration of α-
tocopherol and the combination of allicin and vit. B complex. Moreover, an apparent
decrease in the number of GFAP-positive astrocytes was obvious in the protected groups.
Conclusions: Although both α-tocopherol and the combination of allicin and vit. Bcomplex can be used as possible adjuvant therapies to ameliorate nervous system ailments
attributable to lead acetate, α-tocopherol showed more protective potential.
Key words: Allicin, Purkinje cells, Astrocytes, GFAP, Oligodendrocyte, Myelin Figure
ANTIOXIDANT ACTIVITY AND HEPATOPROTECTIVE EFFECTOF POMEGRANATE PEEL AND WHEY...Anurag Raghuvanshi
The antioxidant activity of pomegranate peel powder (PPP) and whey powder (WP) was evaluated, their hepatoprotective effect of each alone or in combination (PPWP) at equal levels was also evaluated in Wistar rats against carbon tetrachloride (CCL4) induced liver injury.
The hepatoprotective activity was assessed using various biochemical parameters and histopathological studies.
Comparative Study of The Antioxidant Activities of Monodora Myristica And A. ...iosrjce
IOSR Journal of Biotechnology and Biochemistry (IOSR-JBB) covers studies of the chemical processes in living organisms, structure and function of cellular components such as proteins, carbohydrates, lipids, nucleic acids and other biomolecules, chemical properties of important biological molecules, like proteins, in particular the chemistry of enzyme-catalyzed reactions, genetic code (DNA, RNA), protein synthesis, cell membrane transport, and signal transduction. IOSR-JBB is privileged to focus on a wide range of biotechnology as well as high quality articles on genetic engineering, cell and tissue culture technologies, genetics, microbiology, molecular biology, biochemistry, embryology, cell biology, chemical engineering, bioprocess engineering, information technology, biorobotics.
Hepatoprotective activity of aqueous extract of Hibiscus Sabdariffa on alcoho...Bhavana Gundavarapu
The aim of present study was to investigate the Hepatoprotective activity of aqueous extract of Hibiscus sabdariffa (Malvaceace) leaves in albino rats on alcohol induced hepatotoxic activity. .
Does allicin combined with vitamin B-complex have superior potentials than al...Prof. Hesham N. Mustafa
BACKGROUND:
The current article aims to explore the protective potentials of α-tocopherol alone and the combination of allicin and vitamin B-complex against lead-acetate neurotoxicity on the cerebellar cortex.
MATERIALS AND METHODS:
Forty rats were divided into four groups (n=10). Group 1 was the control group. Group 2 received 10 mg/kg body weight (BW) of lead acetate. Group 3 was exposed to 10 mg/kg BW of lead acetate plus a combination of allicin (100 mg/kg BW) and vit. B-complex (40 mg/kg BW). Group 4 was administered lead acetate (10 mg/kg BW) and α-tocopherol (100 mg/kg BW). The animals received treatment for sixty days by oral gavage. All the groups were studied ultrastructurally and immunohistochemically with glial fibrillary acidic protein (GFAP).
RESULTS:
The affected groups revealed shrunken and degenerated Purkinje cells with irregular nuclei. The cytoplasm comprised several lysosomes, unhealthy mitochondria, and dilated Golgi saccules. The myelinated nerve fibers demonstrated breaking of the myelin sheaths, apparent vacuoles, and broad axonal spaces. Immunohistochemically, there was a tremendous surge in GFAP-positive astrocytes in the lead acetate-treated group. These histological and ultrastructural variations were ameliorated by the administration of α-tocopherol and the combination of allicin and vit. B complex. Moreover, an apparent decrease in the number of GFAP-positive astrocytes was obvious in the protected groups.
CONCLUSIONS:
Although both α-tocopherol and the combination of allicin and vit. B-complex can be used as possible adjuvant therapies to ameliorate nervous system ailments attributable to lead acetate, α-tocopherol showed more protective potential.
KEYWORDS:
Allicin; Astrocytes; GFAP; Myelin Figure; Oligodendrocyte; Purkinje cells
Does allicin combined with vitamin B-complex have superior potentials than α-...Prof. Hesham N. Mustafa
Background: The current article aims to explore the protective potentials of α-tocopherol
alone and the combination of allicin and vitamin B-complex against lead-acetate
neurotoxicity on the cerebellar cortex.
Materials and methods: Forty rats were divided into four groups (n=10). Group 1 was the
control group. Group 2 received 10 mg/kg body weight (BW) of lead acetate. Group 3 was
exposed to 10 mg/kg BW of lead acetate plus a combination of allicin (100 mg/kg BW)
and vit. B-complex (40 mg/kg BW). Group 4 was administered lead acetate (10 mg/kg
BW) and α-tocopherol (100 mg/kg BW). The animals received treatment for sixty days by
oral gavage. All the groups were studied ultrastructurally and immunohistochemically with
glial fibrillary acidic protein (GFAP).
Results: The affected groups revealed shrunken and degenerated Purkinje cells with
irregular nuclei. The cytoplasm comprised several lysosomes, unhealthy mitochondria, and
dilated Golgi saccules. The myelinated nerve fibers demonstrated breaking of the myelin
sheaths, apparent vacuoles, and broad axonal spaces. Immunohistochemically, there was a
tremendous surge in GFAP-positive astrocytes in the lead acetate-treated group. These
histological and ultrastructural variations were ameliorated by the administration of α-
tocopherol and the combination of allicin and vit. B complex. Moreover, an apparent
decrease in the number of GFAP-positive astrocytes was obvious in the protected groups.
Conclusions: Although both α-tocopherol and the combination of allicin and vit. Bcomplex can be used as possible adjuvant therapies to ameliorate nervous system ailments
attributable to lead acetate, α-tocopherol showed more protective potential.
Key words: Allicin, Purkinje cells, Astrocytes, GFAP, Oligodendrocyte, Myelin Figure
ANTIOXIDANT ACTIVITY AND HEPATOPROTECTIVE EFFECTOF POMEGRANATE PEEL AND WHEY...Anurag Raghuvanshi
The antioxidant activity of pomegranate peel powder (PPP) and whey powder (WP) was evaluated, their hepatoprotective effect of each alone or in combination (PPWP) at equal levels was also evaluated in Wistar rats against carbon tetrachloride (CCL4) induced liver injury.
The hepatoprotective activity was assessed using various biochemical parameters and histopathological studies.
Comparative Study of The Antioxidant Activities of Monodora Myristica And A. ...iosrjce
IOSR Journal of Biotechnology and Biochemistry (IOSR-JBB) covers studies of the chemical processes in living organisms, structure and function of cellular components such as proteins, carbohydrates, lipids, nucleic acids and other biomolecules, chemical properties of important biological molecules, like proteins, in particular the chemistry of enzyme-catalyzed reactions, genetic code (DNA, RNA), protein synthesis, cell membrane transport, and signal transduction. IOSR-JBB is privileged to focus on a wide range of biotechnology as well as high quality articles on genetic engineering, cell and tissue culture technologies, genetics, microbiology, molecular biology, biochemistry, embryology, cell biology, chemical engineering, bioprocess engineering, information technology, biorobotics.
Protective effects of commelina benghalensis linn (root) extract on ethanol i...IJSIT Editor
The present study was undertaken to investigate the protective effect and possible mechanism of
alcoholic (AlE) and aqueous extract (AqE) from Commelina benghalensis root (CB) on EtOH-induced hepatic
injury in Wistar rat. Hepatotoxic parameters studied in vivo include serum transaminases (AST, and ALT),
ALP, bilirubin, protein, lipid profile (Cholesterol, triglyceride, VLDL and HDL) and level of antioxidants
together with histopathological examination. Liv 52® was used as a reference hepatoprotective agent
(5ml/kg-1b.w.). AlE and AqE (200 mg/kg-1b.w.) on oral administration decreased the level of AST, ALP, ALT,
bilirubin, cholesterol, triglyceride, VLDL, MDA and increased the level of protein, HDL and antioxidants (SOD,
GSH and CAT) in rats being treated with ethanol (EtOH). Pentobarbitone -induced sleeping time study was
carried out to verify the effect on microsomal enzymes Histopathological observations confirmed the
beneficial roles of MF against EtOH-induced liver injury in rats. Possible mechanism may involve their
antioxidant activity
patulin is a secondary metabolite and know about its properties, physiological functions, importance of patulin and its biosynthesis . Types of patulin toxicity and its symptoms, detection and control measures of patulin.
Whey protein products and their combination with L-methionine prevent liver f...iosrphr_editor
The IOSR Journal of Pharmacy (IOSRPHR) is an open access online & offline peer reviewed international journal, which publishes innovative research papers, reviews, mini-reviews, short communications and notes dealing with Pharmaceutical Sciences( Pharmaceutical Technology, Pharmaceutics, Biopharmaceutics, Pharmacokinetics, Pharmaceutical/Medicinal Chemistry, Computational Chemistry and Molecular Drug Design, Pharmacognosy & Phytochemistry, Pharmacology, Pharmaceutical Analysis, Pharmacy Practice, Clinical and Hospital Pharmacy, Cell Biology, Genomics and Proteomics, Pharmacogenomics, Bioinformatics and Biotechnology of Pharmaceutical Interest........more details on Aim & Scope).
Loperamide (LOP) is an antidiarrheal agent that works by slowing gastrointestinal transit and reducing intestinal secretions. The aim of the study is to evaluate the effect of loperamide consumption for five days on the intestinal oxidative balance, as well as the putative protective effect of mallow leaves extract. Animals were divided into one normal control group and fi ve experimental groups. LOP, LOP + the different doses of the extract (100, 200, and 400 mg/ kg, b.w.), and LOP+ yohimbine (2 mg/ kg, b.w. p.i.), used as reference drug. Loperamide (3 mg/ kg, b.w. p.o) was administered twice a day, for 5 days. Treatment with mallow extract or yohimbine protected against the lipid peroxidation, antioxidant enzymes activity depletion, the fall in the thiol group and reduced glutathione level as well as jejunal free iron and H2O2 overload induced by loperamide intoxication. Thereby, Malva sylvestris aqueous extract (MSAE) attenuates the pathogenicity of loperamide.
Protective effects of commelina benghalensis linn (root) extract on ethanol i...IJSIT Editor
The present study was undertaken to investigate the protective effect and possible mechanism of
alcoholic (AlE) and aqueous extract (AqE) from Commelina benghalensis root (CB) on EtOH-induced hepatic
injury in Wistar rat. Hepatotoxic parameters studied in vivo include serum transaminases (AST, and ALT),
ALP, bilirubin, protein, lipid profile (Cholesterol, triglyceride, VLDL and HDL) and level of antioxidants
together with histopathological examination. Liv 52® was used as a reference hepatoprotective agent
(5ml/kg-1b.w.). AlE and AqE (200 mg/kg-1b.w.) on oral administration decreased the level of AST, ALP, ALT,
bilirubin, cholesterol, triglyceride, VLDL, MDA and increased the level of protein, HDL and antioxidants (SOD,
GSH and CAT) in rats being treated with ethanol (EtOH). Pentobarbitone -induced sleeping time study was
carried out to verify the effect on microsomal enzymes Histopathological observations confirmed the
beneficial roles of MF against EtOH-induced liver injury in rats. Possible mechanism may involve their
antioxidant activity
patulin is a secondary metabolite and know about its properties, physiological functions, importance of patulin and its biosynthesis . Types of patulin toxicity and its symptoms, detection and control measures of patulin.
Whey protein products and their combination with L-methionine prevent liver f...iosrphr_editor
The IOSR Journal of Pharmacy (IOSRPHR) is an open access online & offline peer reviewed international journal, which publishes innovative research papers, reviews, mini-reviews, short communications and notes dealing with Pharmaceutical Sciences( Pharmaceutical Technology, Pharmaceutics, Biopharmaceutics, Pharmacokinetics, Pharmaceutical/Medicinal Chemistry, Computational Chemistry and Molecular Drug Design, Pharmacognosy & Phytochemistry, Pharmacology, Pharmaceutical Analysis, Pharmacy Practice, Clinical and Hospital Pharmacy, Cell Biology, Genomics and Proteomics, Pharmacogenomics, Bioinformatics and Biotechnology of Pharmaceutical Interest........more details on Aim & Scope).
Loperamide (LOP) is an antidiarrheal agent that works by slowing gastrointestinal transit and reducing intestinal secretions. The aim of the study is to evaluate the effect of loperamide consumption for five days on the intestinal oxidative balance, as well as the putative protective effect of mallow leaves extract. Animals were divided into one normal control group and fi ve experimental groups. LOP, LOP + the different doses of the extract (100, 200, and 400 mg/ kg, b.w.), and LOP+ yohimbine (2 mg/ kg, b.w. p.i.), used as reference drug. Loperamide (3 mg/ kg, b.w. p.o) was administered twice a day, for 5 days. Treatment with mallow extract or yohimbine protected against the lipid peroxidation, antioxidant enzymes activity depletion, the fall in the thiol group and reduced glutathione level as well as jejunal free iron and H2O2 overload induced by loperamide intoxication. Thereby, Malva sylvestris aqueous extract (MSAE) attenuates the pathogenicity of loperamide.
The Protective Role Of High Dietary Protein On Arsenic Induced Hepatotoxicity...IOSR Journals
The objective of the present investigation was to study the protective role of High dietary protein on arsenic induced hepatotoxicity model in adult male albino rats. Hepatotoxicity in rats was caused by arsenic tri oxide at a dose of 3mg- /ml/kg body weight. Hepamerz, a drug used as standard hepatoprotective agent, was administered orally as standard hepatoprotective agent for 14 consecutive days prior to arsenic treatment at a dose of 10mg- /ml/kg body weight. This drug has many side effects. These side effects have prompted the scientific world for the search of alternative natural remedies of liver damage. The High dietary protein was administered orally to rats along with arsenic. The biochemical parameters were investigated. The results indicated that biochemical changes produced by arsenic were restored to almost normal by High protein diet. The High protein diet produced hepatoprotective effect through the modulation of antioxidant - mediated mechanism by altering serum glutamate oxaloacetate transaminase (SGOT), serum glutamate pyruvate transaminase (SGPT), alkaline phosphatase (ALP), superoxide dismutase (SOD) and catalase (CAT) activities and reduced glutathione (GSH) and lipid peroxidation (LPO) levels - against arsenic induced hepatotoxicity model in rats.
Biorefinery as a possibility to recover aromatic compounds with biological p...Valentin Popa
The biomass can be processed by biorefining with the possibility to separate all chemical compounds among them being polyphenols which can be used as antioxidants
Antioxidant potentials of tannic acid on lipid peroxidation induced by severa...Premier Publishers
Various prospective studies have indicated the antioxidant potency of tannic acid in several models. However, there is no clear-cut evidence revealing that the reported antioxidant properties of tannic acid remains potent regardless of the lipid sources and pro-oxidants employed for the oxidative assault. Hence, this study sought to investigate the antioxidant properties of tannic acid against cerebral and hepatic lipid peroxidation induced by several pro-oxidants (Iron (II) sulfate, Sodium nitroprusside, cyclophosphamide and acetaminophen) in vitro. Rats were decapitated under mild ether anesthesia and the tissues were rapidly dissected, placed on ice, weighed and immediately homogenized in cold 50 mM Tris-HCl, pH 7.4 (1/10, w/v). The homogenates were centrifuged for 10 min at 4000 g to yield a pellet that was discarded and a low-speed supernatant (S1). Our results indicated that Fe (II) showed the highest pro-oxidative effects in both tissues lipids. Furthermore, tannic acid demonstrated potent inhibitory effects against lipid peroxidation in both tissues lipids regardless of the pro-oxidant employed. To this end, there is a dire need to exploit the protective benefits of tannic acid as a potential exogenous antioxidant against lipid peroxidation with a view to providing solution to the global oxidative stress menace.
Polygonum Persicaria (Linn.) and its Active Principle have a hepatoprotective...AI Publications
The aim of this analysis was to see whether the aqueous extract of the roots of Polygonum persicaria (PP) and its active principle, Tannic Acid (TA), had a hepatoprotective and antioxidant effect in rats provided Carbon tetrachloride (1.5 ml/kg, i.p). Twenty albino wistar rats were divided into five groups: control, CCl4-induced hepatotoxicity, hepatotoxicity with Polygonum persicaria and Tannic acid, and a normal group given 100 mg/kg silymarin. After 14 days, the rats were sacrificed. Toxicity testing was carried out on 12 rats. They were randomly allocated to one of three groups: control, Polygonum persicaria 200 mg/kg (B.wt), and Tannic acid 200 mg/kg (B.wt). The amounts of liver homogenate enzymes (glutathione peroxidase, glutathione-S-transferase, glucose-6-phosphatase dehydrogenase, and glutathione reductase enzymes) were greatly restored by extracts of PP and TA at the tested concentrations, supporting the biochemical results. Tannic acid, in contrast to Polygonum persicaria, tends to have a greater liver defensive role toward carbon tetrachloride-induced hepatotoxicity, as well as antioxidant properties and mild anticancer activity against cell viability at higher concentrations. The histological alterations in the liver indicated the injury. Polygonum persicaria & its active principle Tannic acid has strong antioxidant properties as well as hepatoprotective effects against CCl4-induced hepatotoxicity, as demonstrated by these observations.
Effect of astaxanthin on ethylene glycol induced nephrolithiasisIOSR Journals
Nephrolithiasis is one of the most common and painful of urological disorders with a high prevalence rate. The role of calcium oxalate crystals, which are the predominant component of kidney stones in generating oxidative stress, have been clearly demonstrated in previous studies. Astaxanthin, found in marine organisms is a dietary xanthophyll carotenoid with enhanced antioxidative properties and pharmacological effects. In the present study, we have investigated the effect of this natural antioxidant, at a daily dose of 25mg/kg in experimental calcium oxalate nephrolithiasis in male Wistar rats. Liver function markers, hepatic antioxidants, albumin creatinine ratios, renal calcium content and changes in body and kidney weight have been studied to evaluate the effect of this carotenoid in vivo. The effect of citrate, a component of most pharmaceutical drugs for management of nephrolithiasis has also been evaluated for the purpose of comparison with astaxanthin treatment. Astaxanthin is seen to exert a protective effect on the liver and kidney tissues in ethylene glycol treated rats by improving the liver function, restoring the activity of the hepatic antioxidant enzymes, decreasing the albumin creatinine ratios and calcium levels and maintaining the organ to body weight ratio. Our results also indicate that astaxanthin administration is more beneficial than citrate treatment
Weight reduction with improvement of serum lipid profile and ratios of Sesamu...lukeman Joseph Ade shittu
Development of novel natural dietary adjunct/agent with significant therapeutic effects on metabolic disease conditions such as obesity and heart disease raises concern in recent times. We studied chronic toxicity of the combined active ingredients present in the sesame leaves and their interaction on the fasting serum lipid profiles with their ratios in thirty adult male non-obese Sprague Dawley (SD) rats. Such that, both treated groups received 14.0 and 28.0 mg/kg body weight doses of aqueous leaves extract of Sesamum radiatum respectively on a daily basis via gastric gavage, while, the control received equal volume of normal saline daily for six weeks. Significant (P < 0.05) weight loss observed in the treated groups was associated with significant (P < 0.05) reductions in both serum cholesterol and triglycerides (TG). The effect on the serum lipoprotein cholesterol components and ratios were significant in a dose related manner, such that increase in HDL accompanied a corresponding decrease in both LDL and LDL/HDL ratio. In addition, to increased in TG/HDL ratio. However, no significant differences in the relative reduction level of VLDL and triglycerides in the treated groups were found compared to control. Thus, LDL/HDL ratio is significantly a better indicator than the TG/HDL ratio in assessing the impact of sesame treatment with evidence of weight loss and hypolipidaemia especially in hypertensive heart diseases.
Phytochemical Profile and in vitro and in vivo Anticonvulsant and Antioxidant...Self-employed researcher
This study presents the phytochemical profile and in vitro and in
vivo anticonvulsant and antioxidant activities of Epilobium hirsutum, which
has been traditionally used in the treatment of epilepsy by local people of
Turkey. In vitro studies revealed that the extract contained a pronounced
amount of phenolics (206.3±0.9 mg Gallic acid Eq/g extract) and exhibited
significant levels of antioxidant (FRAP; 6226 µmol Fe2+/g extract, ORAC;
6593 µmol Trolox Eq/g extract, DPPH; IC50:33.8 ug/mL and metal chelation;
IC50:114 ug/mL) and anticonvulsant (AChE; IC50:71.2 ug/mL, BChE; IC50:92.5
ug/mL, GABA-T; IC50:94.7 ug/mL) activities. In vivo studies shown that the
the extract exhibited high anticonvulsant activities. In addition, the extracts
regulated the behavior, locomotion, and mental activities of the mice tested.
Biochemical evaluation of the brain tissue revealed that the extract inhibited
the production of MDA and stimulated the increase of antioxidant enzyme
levels, which suggest the possible antioxidative role of the extract that worked
as neuroprotective agents by scarfing the free radicals produced through PTZ
seizure inducer and attenuate convulsions. Moreover the extract regulated
serum biochemical parameters, total antioxidants, total oxidant, and ischemia modified albumin levels. Chromatographic studies were revealed that gallic
acid principally might be the major contributor of anticonvulsant and
antioxidant activities with the additive contributions of fatty acids and mineral
compounds. Findings obtained from this study partially justified the traditional
use of Epilobium hirsutum in the treatment of epilepsy and suggest potential
use of the extract as an industrial or pharmaceutical agent.
1. This article was published in an Elsevier journal. The attached copy
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2. Author's personal copy
Alterations in antioxidant status of rats following intake
of ginger through diet
Nirmala Kota *, Prasanna Krishna, Kalpagam Polasa
Food and Drug Toxicology Research Centre, National Institute of Nutrition (ICMR), PO Jamai Osmania, Hyderabad 500 007, India
Received 2 March 2007; received in revised form 21 May 2007; accepted 28 July 2007
Abstract
Ginger is known to possess potent antioxidant and anti-inflammatory properties. Therefore, in vivo studies in rats were initiated to
investigate if ginger fed through diet can improve their antioxidant status. Inbred male Wistar/NIN rats were divided into four groups
containing six animals per group. The 1st group received a stock diet whereas the 2nd, 3rd and 4th groups were fed with a diet incor-
porating ginger powder at 0.5%, 1% and 5% levels for a period of one month. After one month of feeding, rats were sacrificed and their
livers and kidneys collected for the analysis of antioxidant enzymes like superoxide dismutase (SOD), catalase and glutathione peroxidase
(GSHPx), and to estimate lipid peroxidation and protein oxidation. The levels of all the three enzymes, which are the important com-
ponents of antioxidative defenses, were significantly stimulated in the livers of groups fed with ginger. The significant reduction in lipid
peroxidation in livers and kidneys and inhibition of protein oxidative products in livers indicated the antioxidant potential of ginger
when consumed naturally through diet. The findings reported suggest that regular intake of ginger through diet can protect against oxi-
dative tissue damage.
Ó 2007 Elsevier Ltd. All rights reserved.
Keywords: Ginger; Antioxidant enzymes; Superoxide dismutase (SOD); Catalase; Glutathione peroxidase (GSHPx); Thiobarbituric acid reactive subs-
tances (TBARS)
1. Introduction
Reactive oxygen species (ROS) are generated spontane-
ously in the living cell during metabolism. Antioxidants act
as a major defense against ROS mediated toxicity by pro-
tecting membrane and cytosolic compounds. These com-
pounds exhibit a wide spectrum of activity against
toxicants/carcinogens and have the capacity to intervene
in carcinogen metabolism. The antioxidant defenses
include natural and synthetic antioxidants and the antiox-
idant enzymes present in the biological system (Sies, 1991).
Plant products exert their antioxidant effect by quenching
free radicals. By reducing exposure to free radicals and
by increasing intake of antioxidant nutrients it is possible
to reduce the risk due to free radical health problems asso-
ciated with the aging process, cancer and atherosclerosis
(Ames, Shigenaga, & Hagen, 1993).
Currently there is a strong interest in the study of natu-
ral compounds with free radical scavenging capacity. Die-
tary antioxidants reduce free radical formation and as a
consequence oxidative stress in general, by way of counter-
ing LDL oxidation and platelet aggregation and by inhibi-
tion of the synthesis of proinflammatory cytokines (Kushi
et al., 1996). Lipid peroxidation in tissues results in the pro-
duction and propagation of free radical reactions primarily
involving membrane polyunsaturated fatty acids (PUFAs).
This has been implicated in the pathogenesis of numerous
diseases including atherosclerosis, diabetes, cancer and
rheumatoid arthritis as well as in drug associated toxicity
and aging (Halliwell & Gutteridge, 1989).
Oxidative stress causes damage to nucleic acids, mem-
brane lipids and proteins. Proteins are susceptible to oxida-
tion by ROS, where the type of damage is characteristic of
the denaturing species. The formation of protein carbonyls
0308-8146/$ - see front matter Ó 2007 Elsevier Ltd. All rights reserved.
doi:10.1016/j.foodchem.2007.07.073
*
Corresponding author. Tel.: +91 40 27008921; fax: +91 40 27019074.
E-mail address: knimy7@yahoo.com (N. Kota).
www.elsevier.com/locate/foodchem
Food Chemistry 106 (2008) 991–996
Food
Chemistry
3. Author's personal copy
is considered as a biomarker of primary oxidative stress
(Griffiths, 2001). Oxidative modification of proteins may
occur via metal-catalyzed oxidation, radiation mediated
oxidation and oxidation by oxides of nitrogen. Increase
in carbonyl levels has been detected in several diseases such
as rheumatoid arthritis, cardiovascular diseases etc. (Rez-
nick, Witt, Matsumoto, & Packer, 1992). Metal catalysed
protein oxidation leading to an increase in carbonyl forma-
tion has also been detected in aged rat livers (Starke-Reed
& Oliver, 1989).
The antioxidant enzymes in the tissues can effectively
scavenge free radicals that are generated during xenobiotic
metabolism (Perctval, 1998). Since ancient times spices
have been added to different types of food to improve the
flavour. Now it is well known that spices possess antioxi-
dant activity and prevent oxidation of lipids in foodstuffs.
Kikusaki and Nakatani (1993) reported that chemical con-
stituents like gingerols and shogaols present in ginger
exhibited strong antioxidative activity. Sekiwa, Kubota,
and Kobayashi (2000) reported that novel glucosides
related to gingerdiol from ginger has antioxidative activity
using the linoleic acid model system and by their DPPH
radical scavenging ability. Gingerol, the pungent factor in
ginger oleoresin, inhibited phospholipid peroxidation
induced by the FeCl3 ascorbate system (Aeschbach et al.,
1994). These in vitro observations prompted us to investi-
gate if ginger consumption through diet can improve the
in vivo antioxidant status. Therefore, the levels of antioxi-
dant enzymes, namely, SOD, catalase and GSHPx (Cytosol
and RBC) were measured. Further inhibition of lipid per-
oxidation and protein oxidation was estimated in tissues
of rats (liver and kidney) fed with ginger through their diet
for one month.
2. Materials and methods
2.1. Reagents
All chemicals were of analytical grade. Dimethyl tri-
amine penta acetic acid (DTPA), Tris HCl buffer, Pyrogal-
lol, Beta nicotinamide adenine dinucleotide phosphate
reduced (b-NADPH), Glutathione (GSH), Glutathione
reductase, 2-thiobarbituric acid (TBA), 1,1,3,3-tetra ethoxy
propane (TEP), 2,4-dinitrophenyl hydrazine(DNPH), gua-
nidine hydrochloride were purchased from Sigma Chemical
Co. (St.Louis, MO, USA). All other general chemicals were
of highest purity available in the local market.
2.2. Preparation of test diets
Ginger was purchased from a local market, peeled,
washed, coarsely minced, air dried and pulverized with a
blender to a fine powder. This was added (0.5 g), 1.0 g and
5 g to the stock diet and mixed so as to yield diets of three
compositions containing 0.5%, 1% and 5% ginger, respec-
tively. The amount of ginger powder present in the 15 g diet
which the rat consumed daily corresponds to 0.075, 0.15 and
0.75 g which in turn corresponds to 0.25 g, 0.5 g and 2.5 g of
fresh ginger/15 g of rat diet. The stock diet contained wheat
flour 15%, roasted Bengal gram flour 58%, groundnut flour
10%, skimmed milk powder 5%, casein 4%, refined oil 4%,
salt mixture 4% and vitamin mixture 0.2%.
2.3. Study design
The procurement of animals for experiment was under-
taken by following the ethical guidelines of the Institutes
Animal Ethical Clearance Committee. Inbred male NIN/
Wistar rats, aged about 8–10 weeks, were used for the study.
Age matched and weight matched rats were divided into four
groups containing 6 animals per group. The control group
received a stock diet throughout the experimental period
whereas the experimental groups were fed with the diet
incorporated with ginger powder at 0.5%, 1% and 5% levels
for a period of one month. The rats were maintained at
22 ± 1.2 °C with 50–55% relative humidity and 12 h light/
dark cycle. Food and water were given ad libitum. The
weekly food intake and the body weight of each animal were
recorded at the beginning and end of the experiment.
At the end of 1 month feeding, blood samples were col-
lected from the orbital plexus. The animals were sacrificed
and the organs, namely the liver and kidney, were collected
and frozen under liquid nitrogen immediately and stored at
À80 °C. The tissues were then minced and a 20% (W/V)
homogenate was prepared in cold buffer using a polytron
homogenizer (Kinematica) for a minute. They were pro-
cessed as per the standard procedures for estimation of
antioxidant parameters.
2.4. Measurement of SOD activity
SOD was estimated in liver cytosol according to the
method of Marklund and Marklund (1974). The method
is indirect and is based on the ability of the enzyme to inhi-
bit O2 dependent auto-oxidation of pyrogallol. The reac-
tion mixture contained Tris HCl buffer (50 mM, pH 8.2),
DTPA (1 mM), cytosol (0.1 ml), the reaction was started
by the addition of pyrogallol (1 mM) in a total volume of
1 ml and the increase in absorbance at 420 nm was fol-
lowed for 30 s. A control was run which had pyrogallol
alone without cytosol (enzyme). One unit of SOD activity
is the amount of the enzyme that inhibits the rate of
auto-oxidation of pyrogallol by 50%. Activity of SOD
was expressed as units/mg protein/min.
2.5. Glutathione peroxidase assay (GSHPx)
(a) GSHPx in the cytosolic fraction of liver was deter-
mined by a modified coupled assay procedure of Paglia
and Valentine (1967). This method measures the rate of
glutathione oxidation by hydrogen peroxide as catalyzed
by GSHPx present in the sample by the addition of endog-
enous glutathione reductase and NADPH that converts
oxidized glutathione (GSSG) to the reduced form. The rate
992 N. Kota et al. / Food Chemistry 106 (2008) 991–996
4. Author's personal copy
of GSSG formation is measured by following the decrease
in absorbance of the reaction mixture at 340 nm as
NADPH is converted to NADP. Selenium dependent
GSHPx was measured using hydrogen peroxide as sub-
strate. The activity of GSHPx was calculated as the amount
of NADPH oxidized per minute using the molar absorp-
tion coefficient of 6.22 Â 10À6
for NADPH. Enzyme activ-
ity was expressed as n-moles of NADPH oxidized per mg
protein per minute.
(b) GSHPx in the whole blood was estimated using the
European Community Food Linked Agro-Industrial
Research (EC-FLAIR) common assay (Belsten & Wright,
1995). The following method is based on the original
method of Paglia and Valentine (1967). This was modified
by the addition of dithiothreitol for enzyme stabilization.
tert-Butyl hydroperoxide 2.5 lM (0.1 ml) which was used
as the initiator was also pre-equilibrated at 37 °C and
was mixed quickly with the above and absorbance was
measured over 2 min at 340 nm in Hitachi Spectrophoto-
meter at 37 °C after allowing 1 min initial lag phase for
the restabilisation of temperature. The mean change in
absorbance/min was calculated for the sample and control.
The units of the activity were calculated as nmol b-
NADPH oxidized/min/mg haemoglobin (Hb) using the
Hb concentration of the sample in mg/ml.
2.6. Catalase activity
The activity of catalase in liver homogenate was mea-
sured according to the method of Aebi (1983). The decom-
position of hydrogen peroxide can be followed directly by
the decrease in absorbance at 240 nm per unit time. Hydro-
gen peroxide was diluted with phosphate buffer to 100 ml.
Ten percent of liver homogenate was centrifuged for
10 min at 700Âg. To an aliquot of the supernatant, ethanol
was added to a final concentration of 0.17 M (0.01 ml eth-
anol/ml) and samples were kept for 30 min in an ice water
bath. After 30 min, Triton X 100 was added to a concentra-
tion of 1%, followed by the addition of cold buffer to
achieve a 100-fold dilution. An aliquot of the diluted
homogenate (50–100 ll) equivalent to 20–100 lg protein
was taken and made upto 2 ml with phosphate buffer and
the reaction was started by the addition of 1 ml of hydro-
gen peroxide. The decrease in absorbance at 240 nm was
followed over 2 min. A control was also run containing
either enzyme or substrate. The activity of catalase was cal-
culated from the change in absorbance at 240 nm. One unit
of catalase activity was calculated using K = (2.303/t)
(logA1/A2), where ‘K’ is the rate constant, ‘t’ is the time
in minutes, A1 is absorbance at time 0 and A2 is absorbance
at 2 min. Specific activity was calculated as K/mg protein.
2.7. Estimation of lipid peroxidation and protein oxidation
Thiobarbituric acid reactive substances (TBARS) for the
measurement of lipid peroxidation in liver and kidney
homogenates of rats was carried out according to the proce-
dure of Wilbur, Bernheim, and Shapiro (1949). Malondial-
dehyde (MDA), which is the end product of lipid
peroxidation, was estimated utilizing its property to react
with TBA. To 1 ml of 20% liver and kidney homogenate
prepared in 1.15% KCl, 0.5 ml of 20% of trichloro acetic
acid (TCA) was added followed by 1 ml of 0.67% TBA. This
was heated in a tightly stoppered tube for 10 min in a boil-
ing water bath. After cooling, the tubes were centrifuged to
separate the precipitated protein. The absorbance of the
pink colour produced was read at 340 nm in a spectropho-
tometer. The amount of MDA produced was calculated
using 1,3,3-tetra ethoxy propane as a standard and the
result of peroxidation was expressed as n-moles of MDA
formed per mg of protein. Protein was estimated by the
method of Lowry, Rosenbrongh, Farr, and Rendall (1951).
2.8. Protein carbonyls in liver
Carbonyl groups were assayed by the method described
by Uchida et al. (1998). Protein samples (50 ll liver cytosol
diluted with water to 500 ll) were added to an equal vol-
ume of 0.1% 2, 4-dinitro phenyl hydrazine in 2 M HCl
and left for 1 h at room temperature. Twenty percent
TCA was added to this mixture and the precipitate
extracted three times with 0.5 ml ethanol/ethyl acetate
(1:1). The precipitates were dissolved in 8 M-guanidine
hydrochloride, 13 mM ethylene diamene tetra acetic acid
(EDTA), 133 mM Tris, pH 7.4 and the carbonyl content
was determined at 370 nm with the use of molar absor-
bance coefficient of 22,000 mol/l per mole of protein.
2.9. Statistical analysis
The data was analyzed by the analysis of variance
(ANOVA). Testing of mean values in different groups
was done by Duncan’s multiple range test. SPSS 10 Win-
dow version was used for the statistical analysis (Middle
Brooks, 1977).
3. Results
3.1. Effect of ginger feeding to rats on in vivo antioxidant
enzymes
Significant increase of liver SOD activity was observed
in the ginger fed groups compared to the control group
that was not fed with ginger (p < 0.05). At 0.5%, 1.0%
and 5.0% ginger feeding a stimulation of 76%, 107% and
141% of hepatic SOD against the control was observed.
There was an increase in the activity of catalase compared
to control (p < 0.05). At 0.5%, 1.0% and 5.0% of ginger
feeding a stimulation of 37%, 65% and 94% against control
was observed. The activity of GSHPx in liver cytosol was
more in ginger fed groups compared to the control
(p < 0.05), and the extent of stimulation observed at
0.5%, 1.0% and 5.0% levels was 11%, 21% and 30%, respec-
tively. The activity of GSHPx in whole blood was also
N. Kota et al. / Food Chemistry 106 (2008) 991–996 993
5. Author's personal copy
found to be significant (p < 0.05) and the percentage stim-
ulation was 17%, 23% and 42%, respectively (Table 1).
3.2. Inhibition of lipid peroxidation in rat liver and kidney
The levels of MDA formed in liver homogenates of rats
fed with ginger at different concentrations, namely 0.5%,
1% and 5%, were reduced significantly (p < 0.05) as com-
pared to the control group that was not fed with ginger.
The percentages of inhibition were 35%, 56% and 59%,
respectively. As was observed in liver, significant reduction
(p < 0.05) in levels of lipid peroxides in kidney was
observed in all the ginger fed groups compared to the con-
trol group. At 0.5%, 1.0% and 5.0% of ginger feeding, an
inhibition of 27%, 45% and 59% was observed (Table 2).
3.3. Inhibition of protein oxidation in rat liver
Reduction in the carbonyl levels was observed at all lev-
els of ginger feeding compared to the control group. How-
ever, the only significant decrease (p < 0.05) was observed
with the 5.0% level of feeding.
Percentages of inhibition at 0.5%, 1% and 5.0% ginger
feeding was 23%, 14% and 36%, respectively compared to
the control group (Table 3).
4. Discussion
Oxidative damage in tissues can be limited by the
defense system of the host. These defenses appear to be
inducible by nutrients/non-nutrients in the diet. In addition
to the protective effects of antioxidant enzymes, consump-
tion of dietary antioxidants through the diet plays an
important modulatory role against endogenous oxidative
damage. Among the defenses are enzymes such as SOD,
catalase and GSHPx. The levels of all the three enzymes,
which are important components of enzymatic antioxida-
tive defenses, were significantly stimulated in liver tissue
of rats fed with ginger at all levels. Levels of these enzymes
modulate the oxidative damage within the tissues which
can be quantitated in terms of lipid peroxidation and pro-
tein oxidation. The in vitro antioxidant activity of gingerol
and other constituents of ginger have been previously
reported by Kikusaki and Nakatani (1993). Inhibition of
xanthine oxidase activity responsible for the generation
of reactive oxygen species, such as superoxide anion has
been documented with gingerol (Chang, Chang, Lu, &
Chiang, 1994).
Low levels of antioxidant enzymes were observed in
human lung cancer tissue as compared to normal lung tissue
suggesting that elevated levels of reactive oxygen metabo-
lites could have resulted in mutation in lung carcinoma cell
DNA and other vital cellular components (Guner, Islekel,
Oxteken, Hazan, & Acikel, 1996). Increased flux of oxy-rad-
icals and loss of cellular redox haemostasis is known to
cause ‘‘oxidative stress”, and DNA damage leading to
tumorigenesis (Cerutti, 1994 & Halliwell, 1994).
Our earlier findings in an in vitro study has shown that
DNA damage was inhibited when induced by benzo(a)pyr-
ene in human peripheral blood lymphocytes of male smok-
ers, male non-smokers and females (Nirmala, Prasanna
Table 1
Antioxidant status in ginger fed rats in liver
Groups SOD (U/mg protein) Catalase (U/mg protein) GSHPx
Cytosol (oxidized/mg prot/min) RBC (U/gm Hb)
Control 2.9 ± 0.5a
40.4 ± 3.5a
332 ± 38.6a
267 ± 35.1a
0.5% Ginger 5.1 ± 1.2b
55.4 ± 10.1b
370 ± 29.9a
312 ± 29.2b
1.0% Ginger 6.1 ± 0.9b
66.7 ± 7.9c
401 ± 27.6b
328 ± 6.8b
5.0% Ginger 7.0 ± 0.9bc
78.4 ± 2.7d
433 ± 23.8c
379 ± 25.8c
Values are Mean ± SD of 6 rats per group. Vertical comparisons between groups: ab, bc, bd, cd, ac, ad are significantly different at p < 0.05 by Duncan’s
multiple range test.
Table 2
Inhibition of lipid peroxidation in rat liver and kidney
Groups Liver Kidney
MDA (nmol/mg protein) Percent inhibition MDA (nmol/mg protein) Percent inhibition
Control 3.4 ± 0.5a
– 2.9 ± 0.5a
–
0.5% Ginger 2.2 ± 0.8b
35 2.1 ± 0.5b
27
1.0% Ginger 1.5 ± 0.3c
56 1.6 ± 0.6c
45
5.0% Ginger 1.4 ± 0.2c
59 1.2 ± 0.6c
59
Values are mean ± SD of 6 rats per group. Vertical comparisons: ab, ac, bc are significantly different at p < 0.0.5 by Duncan’s multiple range test.
Table 3
Inhibition of protein oxidation in rat liver
Groups Protein carbonyls (nmol/mg protein) % Inhibition
Control 2.2 ± 0.9a
–
0.5% Ginger 1.7 ± 0.5a
23
1.0% Ginger 1.9 ± 0.4a
14
5.0% Ginger 1.4 ± 0.4b
36
Values are mean ± SD of 6 rats per group. Control vs. 5% ginger p < 0.05
by Duncan’s multiple range test.
994 N. Kota et al. / Food Chemistry 106 (2008) 991–996
6. Author's personal copy
Krishna, & Polasa, 2007a,b). In vivo antimutagenic effect of
ginger was also observed in rats that were fed with ginger
through the diet (Nirmala et al., 2007a,b). These findings
suggest that the antimutagenic and chemopreventive
potential of ginger could be due to its antioxidant activity.
6-Paradol and 6-dehydroparadol, minor chemical con-
stituents of ginger, inhibited TPA induced ear oedema
and ornithine decarboxylase (ODC) activity and tumor
promotion in mouse skin. Antitumor promotion effects of
6-paradol and 6-dehydroparadol were as efficient as that
of curcumin, one of the well-investigated chemopreventive
agents (Chung, Jung, Surh, Lee, & Park, 2001). The anti-
inflammatory effect is due to the inhibition of the arachi-
donic acid cascade. Gingerol, shogaol and other chemicals
in ginger inhibit prostaglandin and leukotriene biosynthesis
through suppression of 5-lipooxygenase synthetase (Sri-
vastava & Mustafa, 1992). Since tumor promotion is asso-
ciated with oxidative stress and inflammation, agents that
possess antioxidant properties capable of mitigating these
effects can act as antitumor promoters.
The significant reduction in MDA in liver and kidney
tissues of ginger fed rats at 0.5%, 1% and 5% suggests that
the antioxidant status of host tissue can be enhanced by
consuming ginger through the diet. Inhibition of protein
oxidative products, which was measured in the liver, rein-
forces our hypothesis that daily ginger ingestion can result
in improved antioxidant status. Dehydrogingerone, a syn-
thetic analogue of zingerone, showed mild inhibition of
lipid peroxidation by acting as free radical scavenger
(Rajkumar & Rao, 1993). Concomitant dietary feeding of
ginger 1% w/w during administration of malathion
(20 ppm) for 4 weeks significantly attenuated malathion
induced lipid peroxidation and oxidative stress in rats
(Ahmed, Seth, Pasha, & Banerjee, 2000). Strengthening
the body defenses by improving the antioxidant status will
undoubtedly protect against the oxidative stress involved in
the etiology of many chronic diseases.
Low levels of tissue antioxidant enzymes are likely to
result in high levels of tissue damage that are reflected as
lipid peroxides, protein carbonyls, etc. Conversely elevated
levels of antioxidant enzymes would reduce this oxidative
damage to tissues, as has been observed in this study in
terms of decreased lipid peroxides at 1% and 5% levels of
ginger and inhibition of protein carbonyls at 5% level.
Although the literature is replete with reports on in vitro
antioxidant activity of dietary substances, limited informa-
tion is available to demonstrate this protective effect when
consumed naturally through the diet. In a diet survey study
conducted by Thimmayamma et al. (1983) it has been
shown that human intake of ginger is 2.6 g/consumption
unit/day. The level of 5% ginger used in this study corre-
sponds to the levels that are consumed in India. Since these
quantities can be easily incorporated and consumed by
humans, a regular intake of ginger may be suggested to
boost antioxidant status, which in turn will reduce the oxi-
dative damage to tissues involved in etiology of complica-
tions associated with degenerative diseases.
Acknowledgement
The authors wish to thank Spices Board, Cochin, India
for supporting in part by grants. We also thank Mr.A.K.
Rajakumar for his technical help.
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