Biological catalysts like enzymes act as catalysts in cells and are secreted by cells. Enzymes are protein catalysts that have an active site which binds specifically to substrate molecules. The active site facilitates the chemical reaction, converting the substrate to product, without being changed itself. Each enzyme only works on specific substrates. The rate of enzyme reactions is affected by temperature and pH levels, with an optimum level for maximum rate of reaction. Changes beyond this can denature the enzyme and disrupt its active site. Enzymes help drive many essential metabolic reactions in living things.