1) Size-exclusion chromatography (SEC) and strong anion exchange chromatography (SAX) are used to separate enoxaparin oligosaccharides based on size and charge, but some oligosaccharides have similar properties and cannot be fully resolved. 2) Capillary electrophoresis (CE) using histamine in the running buffer exploits differences in histamine binding affinity to provide additional separation. 3) Preliminary results show histamine increases migration time of an enoxaparin tetrasaccharide in CE, and binding constants were calculated from isotherm graphs, confirming histamine interacts with heparin oligosaccharides.