Students from a secondary school in Gdańsk, Poland took part in a biology lab class at the University of Gdańsk where they learned about and used laboratory equipment to isolate DNA from kiwi fruit by passing a sample through an ion exchange membrane and removing liquids to isolate the DNA. The process was difficult but the students enjoyed using professional lab equipment and being able to take samples of the isolated DNA back to observe the next day.

3. ‘Isolation of DNA’ classes at the
University of Gdansk

4. On 22th
April class II A took part in biology
classes at the University. The subject of this
project was isolating DNA from kiwi fruit.
Introduction

6. When we came into the lab we were greeted
by a lecturer. Then we put our aprons on
and the scientist told us about laboratory
equipment which we used.
In the lab

7. Laboratory equipment which we
used
Vortex Separator Electronic
pipette

8. Next, the teacher told us how to use
GENOMIC MINI AX TISSUE - special set
to isolate DNA.
In the lab

9. Column
Receiving
tube
Ion exchange
membrane binding
DNA
The flow of liquid
under the influence of
gravity and capillarity

10. DNA isolation consisted of passing through
the membrane reagents and sample organic
with a special fluid and mixing in special
separator.
In the lab

12. At the end we had to remove water and
residual liquids from the sample.
In the lab

13. We could take ready samples to houses-
(samples were ready to watch next day).
In the lab

15. After classes
The process of DNA isolation is very
arduous, but students were very pleased with
these classes, because they could use the
professional laboratory equipment.

16. Thanks for your attentionThanks for your attention

17. Sources:
• http://wtz.ar.krakow.pl/kbz/wirowki.html
• http://www.meranco.com.pl/produkty/pipeta-elektroniczna-xplorer
• http://www.wirowka-laboratoryjna.adverti.com.pl/item.php?
id=WIRLAB-MPW-031&nazwa=Vortex_BIOSAN_V-1_plus
• http://www.aabiot.com/gravity/
• http://www.scind.org/82/Mindblower/russia-to-build--worlds-first-
dna-databank.html
• https://aphroditessecretcyprus.com/?product=kiwi-dried
• http://www.fototerytorium.pl/14084-info-probowki-z-kolorowym-
plynem-w-drewnianym-stoja.html
• http://www.conrad.pl/Mikroskop-Bresser-Optik-USB,-powi
%C4%99kszenie-40x-1024x.htm?websale8=conrad&pi=825709

18. XX Secondary School in Gdańsk, Poland
Supervision: Agnieszka Jackiewicz
Consultation: Beata Piełowska
Author: Maciej Suchocki