Plant tissuce culture and vegetatively propogated crops f. sarsu
•Download as PPTX, PDF•
0 likes•12 views
This document discusses using plant tissue culture techniques and mutation induction in plant breeding. It provides a quick review of plant tissue culture, which involves growing plant parts like cells or tissues in controlled, sterile conditions on synthetic media. Mutation induction can be applied to in vitro plant cultures to generate genetic variation. The history of plant tissue culture is reviewed, from early experiments in the 19th century to developments in the late 20th century like haploid production and plant regeneration from protoplasts. Facilities for plant tissue culture generally include areas for washing, media preparation and sterilization, and aseptic transfer of cultures.
Report
Share
Report
Share
Recommended
Plant tissuce culture and vegetatively propogated crops dr. rafiq
This document discusses using plant tissue culture techniques to induce mutations in plant breeding. It provides a brief overview of plant tissue culture, explaining that it involves growing plant parts like cells, tissues, or organs in sterile conditions on synthetic media. The document also summarizes the history of plant tissue culture, including key discoveries like the first attempts to culture isolated plant cells in 1902 and the development of widely used MS media in 1962. It notes that mutation induction and plant tissue culture techniques have been widely applied in plant breeding in recent decades.
Sclerotia production a way ahead to morchella cultivation
Morchella, the true morels, belonging to Helvellaceae family of class Ascomycetes, are amongst the most highly prized fungi in the world. Their artificial production is still a challenge, even though patents for their cultivation do exist. The tissue of Morchella sp. was transferred aseptically to Potato Dextrose Agar medium (peeled, sliced and boiled potato, 200 g; dextrose, 20 g; agar, 20 g L-1) to grow hyphae. The mycelium showed fastest growth as compared to other edible mushrooms. It covered entire area of Petri plate (90 mm) within 4-5 days with the growth rate of 18-22.5mm/day. A unique growth pattern i.e. vertically oriented mycelia were
observed. Brown coloured pigmentation in the culture was also observed during the study. The basal media for spawn (wheat grains; Glucose, 1%, CaCO3, 2%; CaSO4, 1.5% and MgSO4, 1%) was aseptically inoculated with the mushroom culture. The spawn substrate was colonized by mushroom mycelium in 7-8 days. The sclerotia were formed in unused (old) spawn. Wheat
straw was used as a substrate for mushroom cultivation. It was supplemented with wheat bran, 20%, Glucose (1%) and MgSO4 (1%). It showed prolific growth when it was seeded by
mushroom spawn using jar method. Once substrate was fully covered with mushroom mycelium, casing was applied. Sclerotia were successfully obtained after 14 days of incubation in our experiment both in the substrate and casing soil but failed to give rise to fruiting primordial. Further research is going on to domesticate this species in this part of country.
Pioneers in plant tissue culture -2
F.C. Steward was a pioneer in plant tissue culture who developed techniques for applications like using coconut water in plant cultures. Street conducted extensive work on root cultures to understand plant growth. Morel revolutionized the orchid industry through micropropagation, cloning plants from a single bud. Vasil and Hildebrandt were the first to prove Schwann's cell theory by regenerating a tobacco plant from a single cell. Cocking demonstrated isolating large numbers of protoplasts through enzymatic degradation of cell walls.
Biopharming_Designer_Crops
Plant biopharming is defined as the farming of transgenic plants genetically modified to produce “humanised” pharmaceutical substances for use in humans.
History of plant tissue culture
Cell and tissue culture refers to growing plant cells, tissues, organs, seeds or plant parts in a sterile nutrient medium in vitro. The history of cell and tissue culture began with Haberlandt in 1898, who first cultured single plant cells and proposed the concept of totipotency. Significant milestones included the discovery of growth regulators like auxin in the 1930s-1940s and development of the MS medium in 1962, allowing continuous cell growth and differentiation in vitro. This led to advances like anther culture in 1964, protoplast isolation in the 1960s, and the first regenerated plant from protoplasts in 1971.
Applications of Plant Tissue Culture
I have discussed Applications of Plant Tissue Culture under the following subheadings,
1. Micro Propagation
2. Clonal Propagation
3. Production of Genetically Variable Plants
4. Production of Virus Free Plants
5. Plant Breeding
6. Production of Useful Biochemicals
7. Preservation of Plant Genetic Resources
8. Importance of Tissue Culture in Biotechnology
Food and hunger ppt
Food provides nutrients that are consumed by organisms for energy, growth, and survival. Plants and animals are the primary sources of food, with around 2,000 cultivated plant species and meat obtained directly from animal muscle and organs. A lack of sufficient food intake results in the condition of hunger. Genetically modified organisms (GMOs) have been engineered through genetic modification techniques, and are used in research, pharmaceuticals, and agriculture through traits like herbicide resistance or increased nutritional value in crops.
Powerpoint lhyn
This document discusses genetically modified organisms (GMOs). It defines a GMO as an organism whose genetic material has been altered using biotechnology. This allows genes to be transferred between species in a way that does not occur naturally. The document outlines both potential advantages and disadvantages of GMOs. Advantages include increased resistance to pests and environmental stresses, higher yields, and helping address issues like food scarcity. Disadvantages include possible allergic reactions and concerns about environmental impacts. The document concludes by arguing that GMOs can help improve food security and that with proper regulation, their benefits can outweigh risks to human health and the environment.
Recommended
Plant tissuce culture and vegetatively propogated crops dr. rafiq
This document discusses using plant tissue culture techniques to induce mutations in plant breeding. It provides a brief overview of plant tissue culture, explaining that it involves growing plant parts like cells, tissues, or organs in sterile conditions on synthetic media. The document also summarizes the history of plant tissue culture, including key discoveries like the first attempts to culture isolated plant cells in 1902 and the development of widely used MS media in 1962. It notes that mutation induction and plant tissue culture techniques have been widely applied in plant breeding in recent decades.
Sclerotia production a way ahead to morchella cultivation
Morchella, the true morels, belonging to Helvellaceae family of class Ascomycetes, are amongst the most highly prized fungi in the world. Their artificial production is still a challenge, even though patents for their cultivation do exist. The tissue of Morchella sp. was transferred aseptically to Potato Dextrose Agar medium (peeled, sliced and boiled potato, 200 g; dextrose, 20 g; agar, 20 g L-1) to grow hyphae. The mycelium showed fastest growth as compared to other edible mushrooms. It covered entire area of Petri plate (90 mm) within 4-5 days with the growth rate of 18-22.5mm/day. A unique growth pattern i.e. vertically oriented mycelia were
observed. Brown coloured pigmentation in the culture was also observed during the study. The basal media for spawn (wheat grains; Glucose, 1%, CaCO3, 2%; CaSO4, 1.5% and MgSO4, 1%) was aseptically inoculated with the mushroom culture. The spawn substrate was colonized by mushroom mycelium in 7-8 days. The sclerotia were formed in unused (old) spawn. Wheat
straw was used as a substrate for mushroom cultivation. It was supplemented with wheat bran, 20%, Glucose (1%) and MgSO4 (1%). It showed prolific growth when it was seeded by
mushroom spawn using jar method. Once substrate was fully covered with mushroom mycelium, casing was applied. Sclerotia were successfully obtained after 14 days of incubation in our experiment both in the substrate and casing soil but failed to give rise to fruiting primordial. Further research is going on to domesticate this species in this part of country.
Pioneers in plant tissue culture -2
F.C. Steward was a pioneer in plant tissue culture who developed techniques for applications like using coconut water in plant cultures. Street conducted extensive work on root cultures to understand plant growth. Morel revolutionized the orchid industry through micropropagation, cloning plants from a single bud. Vasil and Hildebrandt were the first to prove Schwann's cell theory by regenerating a tobacco plant from a single cell. Cocking demonstrated isolating large numbers of protoplasts through enzymatic degradation of cell walls.
Biopharming_Designer_Crops
Plant biopharming is defined as the farming of transgenic plants genetically modified to produce “humanised” pharmaceutical substances for use in humans.
History of plant tissue culture
Cell and tissue culture refers to growing plant cells, tissues, organs, seeds or plant parts in a sterile nutrient medium in vitro. The history of cell and tissue culture began with Haberlandt in 1898, who first cultured single plant cells and proposed the concept of totipotency. Significant milestones included the discovery of growth regulators like auxin in the 1930s-1940s and development of the MS medium in 1962, allowing continuous cell growth and differentiation in vitro. This led to advances like anther culture in 1964, protoplast isolation in the 1960s, and the first regenerated plant from protoplasts in 1971.
Applications of Plant Tissue Culture
I have discussed Applications of Plant Tissue Culture under the following subheadings,
1. Micro Propagation
2. Clonal Propagation
3. Production of Genetically Variable Plants
4. Production of Virus Free Plants
5. Plant Breeding
6. Production of Useful Biochemicals
7. Preservation of Plant Genetic Resources
8. Importance of Tissue Culture in Biotechnology
Food and hunger ppt
Food provides nutrients that are consumed by organisms for energy, growth, and survival. Plants and animals are the primary sources of food, with around 2,000 cultivated plant species and meat obtained directly from animal muscle and organs. A lack of sufficient food intake results in the condition of hunger. Genetically modified organisms (GMOs) have been engineered through genetic modification techniques, and are used in research, pharmaceuticals, and agriculture through traits like herbicide resistance or increased nutritional value in crops.
Powerpoint lhyn
This document discusses genetically modified organisms (GMOs). It defines a GMO as an organism whose genetic material has been altered using biotechnology. This allows genes to be transferred between species in a way that does not occur naturally. The document outlines both potential advantages and disadvantages of GMOs. Advantages include increased resistance to pests and environmental stresses, higher yields, and helping address issues like food scarcity. Disadvantages include possible allergic reactions and concerns about environmental impacts. The document concludes by arguing that GMOs can help improve food security and that with proper regulation, their benefits can outweigh risks to human health and the environment.
Application of biotechnology
This document discusses the application of biotechnology in the food, pharmaceutical, and agriculture industries. It provides examples of how biotechnology is used in food processing, such as developing new emulsifiers and tests for food allergens. In pharmaceuticals, biotechnology has been used to develop vaccines, insulin, blood products, and gene therapies. In agriculture, biotechnology can be applied to increase pest resistance, disease resistance, nutritional quality, and environmental stress tolerance in crops. Genetically modified crops are also discussed.
Genetically Modified Organisms 01
This document discusses genetically modified organisms (GMOs) and transgenic animals. It begins by defining a GMO as an organism whose genetic material has been altered by inserting DNA from another organism. Currently, most GM crops contain bacterial genes for pest or herbicide resistance. The document then discusses three main methods for producing transgenic animals: DNA microinjection, retrovirus-mediated gene transfer, and embryonic stem cell-mediated gene transfer. Transgenic animals have applications in agriculture, medicine, and industry by improving crop yields, providing organs for transplantation, and producing useful proteins.
role of biotechnology in agriculture
Biotechnology with agriculture is very useful in now a days and also in upcoming days. With the help of biotechnology we can produce better quality of crops and also increase the yield. The produces are also free from pests.
Molecular farming
Molecular Farming, green biotechnology, milestones, general strategy, plant expression hosts, advantage & limitation, plant-based expression system, biosafety concern
History of Plant Tissue Culture
1. The history of plant tissue culture began in 1902 when Gottlieb Haberlandt first cultured isolated plant cells in a nutrient solution, hoping to regenerate whole plants, though he was unsuccessful.
2. In the 1930s and 1940s, scientists like White, Gautheret, and Nobecourt established the first continuously growing plant tissue cultures using auxins and vitamins.
3. Advances in the mid-20th century led to developments like cell plating techniques, synthetic media like Murashige and Skoog medium, regeneration of plants from isolated cells and protoplasts, and the first transgenic plants.
Applications of plant biotechnology
The document summarizes applications of plant biotechnology. It discusses using biotechnology to develop plants with improved tolerance to abiotic stresses like drought, salt, and heat through genes that increase osmolyte production, chaperone proteins, and wax production. It also describes developing biotic stress tolerance through viral, bacterial, fungal and insect resistance genes. Additionally, it outlines using plants to produce pharmaceuticals like vaccines administered through edible crops as well as phytochemicals and other therapeutics.
Plant biotechnology
This document provides an introduction to plant biotechnology. It defines plant biotechnology and discusses its origins from early uses of microorganisms in fermentation. It outlines the broad categories of biotechnology and characteristics of technological revolutions. The document also summarizes the history of plant tissue culture, from early experiments in the 1900s to recent developments in genetic engineering. It describes various plant tissue culture techniques including callus, organ, meristem, protoplast and cell culture.
Pioneers in plant tissue culture -1
Gottlieb Haberlandt was the first to attempt culturing single plant cells in nutrient solution but the cells only survived up to 1 month without dividing. P. Nobecourt, P.R. White and R.J. Gautheret announced in 1939 that plant tissues could be cultured indefinitely with the use of indole acetic acid. P.R. White was also a pioneer in plant tissue culture and reported the first successful continuous culture of tomato root tips in 1934. Folke Skoog discovered cytokinins, a major new class of plant hormones, and isolated kinetin which stimulates plant cell division. In 1962, Murashige and Skoog formulated the most widely used plant tissue
Pioneers in plant tissue culture -3
1) Several scientists pioneered the field of plant tissue culture, including Erhard Kranz, Satish Maheshwari, Sipra Guha-Mukherjee, Armin Braun, and Mary-Dell Chilton.
2) In the 1940s-1950s, Braun demonstrated that the tumor-inducing principle of Agrobacterium tumefaciens is transferred to the plant genome during infection and causes autonomous growth of crown gall tissue.
3) In the 1960s, Guha and Maheshwari induced microspores in Datura innoxia anther cultures to form sporophytes, demonstrating haploid development; Guha-M
Bacterial contaminants of plant tissue culture
This document discusses bacterial contaminants in plant tissue culture and methods for controlling contamination. It notes that the culture medium provides nutrients for bacteria and stressed plant tissues can be susceptible to infection. Contaminants may originate from explants, operators, or ineffective sterilization. Procedures to produce aseptic cultures require identifying and characterizing contaminants and eliminating them using improved practices, antibiotics, or other agents. Modern identification techniques include Biolog, API, fatty acid analysis, and 16S rRNA analysis. Common contaminants and effective antibiotic treatments are also discussed.
Media composition and preparation
The document discusses plant tissue culture media. It describes media as a substance that supports and provides energy and nutrients for developing plant explants. The main types of media are solid and liquid, and their composition varies depending on the explant species. Common media include Murashige and Skoog, White's Medium, Gamborg Medium, and Nitsch's Medium. Media contains inorganic supplements like macronutrients and micronutrients as well as organic supplements such as vitamins, amino acids, and energy sources. Other constituents include growth regulators, solidifying agents, and pH buffers. Media is prepared by making stock solutions of dry and wet ingredients, adding a gelling agent, and sterilizing the final mixture.
Future of food biotechnology, Consumer perspectives and Scope in India
This presentation talks about the future of food biotechnology, consumer perspective and its Scope in India
Nithin S A- Genetically modified food
Genetically modified crops are crop plants created through genetic engineering to enhance desirable traits. This document discusses what GM crops are, their history, various techniques used in genetic modification including bacterial carriers, biolistic methods, electroporation, gene splicing and microinjection. It provides examples of GM crops like Bt cotton, herbicide tolerant soybean, virus resistant papaya. The document also discusses GM techniques used to develop cold tolerant tomatoes, vitamin rich tomatoes, and protein enriched potatoes.
Food biotechnology
This document discusses food biotechnology. It begins by defining food and biotechnology. Food biotechnology applications include genetically modifying plants and animals for agriculture, increasing nutrient levels in foods, and developing foods that have longer shelf lives. The document discusses debates around transgenic and genetically modified organisms and potential health risks of consuming genetically modified foods. It concludes by thanking the reader and providing a bibliography.
Plant tissue culture Application
Plant tissue culture:
Historical development of plant tissue culture, types of cultures, Nutritional requirements, growth and their maintenance.
Applications of plant tissue culture in pharmacognosy. For second year B.pharmacy
Tissue culture
Plant tissue culture is the process of producing plants from tissue of a desired plant in a controlled nutrient medium, producing an exact replica of the mother plant. Plant biotechnology genetically modifies plants by manipulating genes in a laboratory and transferring them to a plant to alter its characteristics. The goals of modern plant biotechnology include improving crop production through traits like increased nutrition, disease resistance, insect control, and weed management. Applications include producing golden rice with more vitamin A, plants resistant to viruses, crops containing genes for natural insecticides, and herbicide-resistant crops. Future areas may include caffeine-free coffee, pharmaceuticals, and using plants as biofuel sources.
Conventional and modern biotechnology
The document discusses the differences between conventional and modern biotechnology. Conventional biotechnology uses microorganisms to produce foods through natural processes like fermentation. Modern biotechnology is based on genetic engineering and DNA manipulation to more efficiently produce products. Examples of techniques in modern biotechnology include tissue culture, crossbreeding, artificial insemination, cloning, hydroponics, and aeroponics.
GENETICALLY MODIFIED ORGANISMS
This document discusses genetically modified organisms (GMOs). It defines GMOs as organisms whose genetic material has been altered using genetic engineering, including plants, animals, microbes, and foods. The document outlines the process of genetic modification involving inserting or deleting genes. It describes existing GMO models like transgenic plants engineered for research or improved crops, microbes used to produce medicines, and genetically engineered animals and fish used for research. The document also compares genetic engineering to traditional breeding and discusses both the pros and cons of genetic engineering.
Seminor on fututre biotech
Biotechnology is the application of biological processes and systems to solve problems or make useful products. It includes techniques like genetic engineering, cloning, and cell fusion. India has emerged as a major player in biotechnology, with several biotech clusters and top companies located in cities like Bangalore, Hyderabad, and New Delhi. The Indian government has supported biotechnology growth through agencies and funding. Biotechnology is applied in diverse fields like healthcare, agriculture, industry, and environment.
Role of biotechnology for sustainable AGRICULTURE
THIS SEMINAR HIGHLIGHTS THE ROLE OF GENETIC ENGINEERING AND BIOTECHNOLOGY FOR ACHIEVING THE SUSTAINABLE GOAL FOR 21ST CENTURY AGRICULTURE
$Tissue culture and advanced crop improvement using biotechnology
The document discusses tissue culture techniques and their applications in plant breeding. It provides a historical background of tissue culture dating back to the early 20th century. The major steps involved in tissue culture are explained, including selection of explant tissue, sterilization, establishment in culture medium, multiplication through callus formation, root formation, and hardening of plantlets. Key techniques like protoplast culture, haploid culture, and micropropagation are also summarized along with their achievements in generating somatic hybrids, true breeding lines, and large-scale clonal propagation respectively.
Over Review on Plant Tissue Culture
Plant tissue culture has been widely employed in area of agriculture, horticulture, forestry and plant breeding. It is an applied biotechnology used for mass propagation, virus elimination, secondary metabolite production and in vitro cloning of plants. Recently, plant tissue culture has been used for the conservation of endangered plant species through short and medium term conservation also known as slow growth and cryopreservation also known as long term conservation. These methods had been effectively used to conserve plant species with recalcitrant seeds or dormant seeds and showed greater advantage over the conventional methods of conservation. At present plant cell culture has made great advances. Possibly the most significant role that plant cell culture has to play in the future will be in its association with transgenic plants. The ability to accelerate the conventional multiplication rate can be of great benefit to many crops countries where a disease or some climatic disaster wipes out crops. Mr. Rohan R. Vakhariya | Rutuja R. Shah "Over Review on Plant Tissue Culture" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-4 | Issue-1 , December 2019, URL: https://www.ijtsrd.com/papers/ijtsrd29619.pdfPaper URL: https://www.ijtsrd.com/pharmacy/other/29619/over-review-on-plant-tissue-culture/mr-rohan-r-vakhariya
More Related Content
What's hot
Application of biotechnology
This document discusses the application of biotechnology in the food, pharmaceutical, and agriculture industries. It provides examples of how biotechnology is used in food processing, such as developing new emulsifiers and tests for food allergens. In pharmaceuticals, biotechnology has been used to develop vaccines, insulin, blood products, and gene therapies. In agriculture, biotechnology can be applied to increase pest resistance, disease resistance, nutritional quality, and environmental stress tolerance in crops. Genetically modified crops are also discussed.
Genetically Modified Organisms 01
This document discusses genetically modified organisms (GMOs) and transgenic animals. It begins by defining a GMO as an organism whose genetic material has been altered by inserting DNA from another organism. Currently, most GM crops contain bacterial genes for pest or herbicide resistance. The document then discusses three main methods for producing transgenic animals: DNA microinjection, retrovirus-mediated gene transfer, and embryonic stem cell-mediated gene transfer. Transgenic animals have applications in agriculture, medicine, and industry by improving crop yields, providing organs for transplantation, and producing useful proteins.
role of biotechnology in agriculture
Biotechnology with agriculture is very useful in now a days and also in upcoming days. With the help of biotechnology we can produce better quality of crops and also increase the yield. The produces are also free from pests.
Molecular farming
Molecular Farming, green biotechnology, milestones, general strategy, plant expression hosts, advantage & limitation, plant-based expression system, biosafety concern
History of Plant Tissue Culture
1. The history of plant tissue culture began in 1902 when Gottlieb Haberlandt first cultured isolated plant cells in a nutrient solution, hoping to regenerate whole plants, though he was unsuccessful.
2. In the 1930s and 1940s, scientists like White, Gautheret, and Nobecourt established the first continuously growing plant tissue cultures using auxins and vitamins.
3. Advances in the mid-20th century led to developments like cell plating techniques, synthetic media like Murashige and Skoog medium, regeneration of plants from isolated cells and protoplasts, and the first transgenic plants.
Applications of plant biotechnology
The document summarizes applications of plant biotechnology. It discusses using biotechnology to develop plants with improved tolerance to abiotic stresses like drought, salt, and heat through genes that increase osmolyte production, chaperone proteins, and wax production. It also describes developing biotic stress tolerance through viral, bacterial, fungal and insect resistance genes. Additionally, it outlines using plants to produce pharmaceuticals like vaccines administered through edible crops as well as phytochemicals and other therapeutics.
Plant biotechnology
This document provides an introduction to plant biotechnology. It defines plant biotechnology and discusses its origins from early uses of microorganisms in fermentation. It outlines the broad categories of biotechnology and characteristics of technological revolutions. The document also summarizes the history of plant tissue culture, from early experiments in the 1900s to recent developments in genetic engineering. It describes various plant tissue culture techniques including callus, organ, meristem, protoplast and cell culture.
Pioneers in plant tissue culture -1
Gottlieb Haberlandt was the first to attempt culturing single plant cells in nutrient solution but the cells only survived up to 1 month without dividing. P. Nobecourt, P.R. White and R.J. Gautheret announced in 1939 that plant tissues could be cultured indefinitely with the use of indole acetic acid. P.R. White was also a pioneer in plant tissue culture and reported the first successful continuous culture of tomato root tips in 1934. Folke Skoog discovered cytokinins, a major new class of plant hormones, and isolated kinetin which stimulates plant cell division. In 1962, Murashige and Skoog formulated the most widely used plant tissue
Pioneers in plant tissue culture -3
1) Several scientists pioneered the field of plant tissue culture, including Erhard Kranz, Satish Maheshwari, Sipra Guha-Mukherjee, Armin Braun, and Mary-Dell Chilton.
2) In the 1940s-1950s, Braun demonstrated that the tumor-inducing principle of Agrobacterium tumefaciens is transferred to the plant genome during infection and causes autonomous growth of crown gall tissue.
3) In the 1960s, Guha and Maheshwari induced microspores in Datura innoxia anther cultures to form sporophytes, demonstrating haploid development; Guha-M
Bacterial contaminants of plant tissue culture
This document discusses bacterial contaminants in plant tissue culture and methods for controlling contamination. It notes that the culture medium provides nutrients for bacteria and stressed plant tissues can be susceptible to infection. Contaminants may originate from explants, operators, or ineffective sterilization. Procedures to produce aseptic cultures require identifying and characterizing contaminants and eliminating them using improved practices, antibiotics, or other agents. Modern identification techniques include Biolog, API, fatty acid analysis, and 16S rRNA analysis. Common contaminants and effective antibiotic treatments are also discussed.
Media composition and preparation
The document discusses plant tissue culture media. It describes media as a substance that supports and provides energy and nutrients for developing plant explants. The main types of media are solid and liquid, and their composition varies depending on the explant species. Common media include Murashige and Skoog, White's Medium, Gamborg Medium, and Nitsch's Medium. Media contains inorganic supplements like macronutrients and micronutrients as well as organic supplements such as vitamins, amino acids, and energy sources. Other constituents include growth regulators, solidifying agents, and pH buffers. Media is prepared by making stock solutions of dry and wet ingredients, adding a gelling agent, and sterilizing the final mixture.
Future of food biotechnology, Consumer perspectives and Scope in India
This presentation talks about the future of food biotechnology, consumer perspective and its Scope in India
Nithin S A- Genetically modified food
Genetically modified crops are crop plants created through genetic engineering to enhance desirable traits. This document discusses what GM crops are, their history, various techniques used in genetic modification including bacterial carriers, biolistic methods, electroporation, gene splicing and microinjection. It provides examples of GM crops like Bt cotton, herbicide tolerant soybean, virus resistant papaya. The document also discusses GM techniques used to develop cold tolerant tomatoes, vitamin rich tomatoes, and protein enriched potatoes.
Food biotechnology
This document discusses food biotechnology. It begins by defining food and biotechnology. Food biotechnology applications include genetically modifying plants and animals for agriculture, increasing nutrient levels in foods, and developing foods that have longer shelf lives. The document discusses debates around transgenic and genetically modified organisms and potential health risks of consuming genetically modified foods. It concludes by thanking the reader and providing a bibliography.
Plant tissue culture Application
Plant tissue culture:
Historical development of plant tissue culture, types of cultures, Nutritional requirements, growth and their maintenance.
Applications of plant tissue culture in pharmacognosy. For second year B.pharmacy
Tissue culture
Plant tissue culture is the process of producing plants from tissue of a desired plant in a controlled nutrient medium, producing an exact replica of the mother plant. Plant biotechnology genetically modifies plants by manipulating genes in a laboratory and transferring them to a plant to alter its characteristics. The goals of modern plant biotechnology include improving crop production through traits like increased nutrition, disease resistance, insect control, and weed management. Applications include producing golden rice with more vitamin A, plants resistant to viruses, crops containing genes for natural insecticides, and herbicide-resistant crops. Future areas may include caffeine-free coffee, pharmaceuticals, and using plants as biofuel sources.
Conventional and modern biotechnology
The document discusses the differences between conventional and modern biotechnology. Conventional biotechnology uses microorganisms to produce foods through natural processes like fermentation. Modern biotechnology is based on genetic engineering and DNA manipulation to more efficiently produce products. Examples of techniques in modern biotechnology include tissue culture, crossbreeding, artificial insemination, cloning, hydroponics, and aeroponics.
GENETICALLY MODIFIED ORGANISMS
This document discusses genetically modified organisms (GMOs). It defines GMOs as organisms whose genetic material has been altered using genetic engineering, including plants, animals, microbes, and foods. The document outlines the process of genetic modification involving inserting or deleting genes. It describes existing GMO models like transgenic plants engineered for research or improved crops, microbes used to produce medicines, and genetically engineered animals and fish used for research. The document also compares genetic engineering to traditional breeding and discusses both the pros and cons of genetic engineering.
Seminor on fututre biotech
Biotechnology is the application of biological processes and systems to solve problems or make useful products. It includes techniques like genetic engineering, cloning, and cell fusion. India has emerged as a major player in biotechnology, with several biotech clusters and top companies located in cities like Bangalore, Hyderabad, and New Delhi. The Indian government has supported biotechnology growth through agencies and funding. Biotechnology is applied in diverse fields like healthcare, agriculture, industry, and environment.
Role of biotechnology for sustainable AGRICULTURE
THIS SEMINAR HIGHLIGHTS THE ROLE OF GENETIC ENGINEERING AND BIOTECHNOLOGY FOR ACHIEVING THE SUSTAINABLE GOAL FOR 21ST CENTURY AGRICULTURE
What's hot (20)
Future of food biotechnology, Consumer perspectives and Scope in India
Future of food biotechnology, Consumer perspectives and Scope in India
Similar to Plant tissuce culture and vegetatively propogated crops f. sarsu
$Tissue culture and advanced crop improvement using biotechnology
The document discusses tissue culture techniques and their applications in plant breeding. It provides a historical background of tissue culture dating back to the early 20th century. The major steps involved in tissue culture are explained, including selection of explant tissue, sterilization, establishment in culture medium, multiplication through callus formation, root formation, and hardening of plantlets. Key techniques like protoplast culture, haploid culture, and micropropagation are also summarized along with their achievements in generating somatic hybrids, true breeding lines, and large-scale clonal propagation respectively.
Over Review on Plant Tissue Culture
Plant tissue culture has been widely employed in area of agriculture, horticulture, forestry and plant breeding. It is an applied biotechnology used for mass propagation, virus elimination, secondary metabolite production and in vitro cloning of plants. Recently, plant tissue culture has been used for the conservation of endangered plant species through short and medium term conservation also known as slow growth and cryopreservation also known as long term conservation. These methods had been effectively used to conserve plant species with recalcitrant seeds or dormant seeds and showed greater advantage over the conventional methods of conservation. At present plant cell culture has made great advances. Possibly the most significant role that plant cell culture has to play in the future will be in its association with transgenic plants. The ability to accelerate the conventional multiplication rate can be of great benefit to many crops countries where a disease or some climatic disaster wipes out crops. Mr. Rohan R. Vakhariya | Rutuja R. Shah "Over Review on Plant Tissue Culture" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-4 | Issue-1 , December 2019, URL: https://www.ijtsrd.com/papers/ijtsrd29619.pdfPaper URL: https://www.ijtsrd.com/pharmacy/other/29619/over-review-on-plant-tissue-culture/mr-rohan-r-vakhariya
Plant Tissue Culture
Plant tissue culture is a collection of techniques used to maintain or grow plant cells, tissues, and organs under sterile conditions. The history of plant tissue culture began in the 1830s with theories of cell totipotency. Significant developments included the discovery of plant growth regulators in the 1920s-1940s and the development of plant cell differentiation and somatic embryogenesis in the 1950s-1960s. There are several types of plant tissue culture including shoot culture, callus culture, embryo culture, and meristem culture. Applications include germplasm conservation, large-scale production, disease eradication, genetic engineering, and more. The advantages are rapid propagation, disease-free plants, year-round growth, and conservation of endangered
Tissueculture copy-150516181545-lva1-app6891
Plant tissue culture is the process of growing plant cells, tissues or organs in sterile conditions on a nutrient medium. It has many applications like germplasm preservation of endangered plants, producing disease-free plants through micropropagation, and creating novel hybrids through protoplast fusion and somatic hybridization. However, issues remain like genetic instability of hybrids and lack of efficient selection methods. Overall, tissue culture is a valuable biotechnology tool with potential for crop improvement and conservation efforts.
Tissue culture
Plant tissue culture is the process of growing plant cells, tissues or organs in sterile conditions on a nutrient medium. It has many applications like germplasm preservation of endangered plants, genetic improvement of crops, and production of secondary metabolites. The basic steps include selection of explant, initiation of culture on growth media, multiplication through cell division, and rooting and transfer to soil. Technologies such as micropropagation, somatic hybridization, and cryopreservation have been commercialized for mass propagation of crops. However, issues remain regarding genetic stability, selection of fused products, and regeneration efficiency.
Tissue culture terminology.pptx
The document discusses the history and techniques of plant tissue culture. It begins with early experiments in the 1830s culturing plant cells outside of their natural environment. Significant developments include the discovery of growth hormones auxin and cytokinin in the 1950s, and the development of the Murashige and Skoog medium in 1962. The document outlines the basic requirements and procedures for plant tissue culture, including selecting an explant, preparing sterile media, inoculation, incubation, sub-culturing, and transferring plantlets. Applications include rapid clonal propagation, inducing mutations, producing disease-free plants, and conserving rare species.
Introduction to Plant Tissue Culture and Propagation
This document provides an introduction to plant tissue culture and propagation prepared for third year biology students. It defines key terms related to plant tissue culture such as explant, callus, and nutrient medium. The document discusses the history and development of plant tissue culture techniques dating back to early experiments in the 1900s. It also covers the application of plant tissue culture for cloning plants, eliminating diseases, and producing metabolites, as well as factors that affect culture efficiency such as growth media, environmental conditions, and the explant source.
Tissue Culture its types and Applications By Samra Tahir
Plant tissue culture
Animal tissue culture
stages,types,summary,all facts and details of tissue culture.
https://www2.slideshare.net/Samratahir7/all-about-tissue-culture-by-samra-tahir
Kamal presentation
This document provides an introduction to tissue culture applications in fruit crops. It discusses key terms like totipotency and explant. Important contributors to plant tissue culture development are noted, including Haberlandt, Skoog, and Murashige. The history of tissue culture is summarized. Micropropagation techniques are explained in several stages. Various explant sources, types of micropropagation, and applications of tissue culture like clonal propagation are described in detail over multiple pages.
Plant Tissue Culture..“Micropropagation Studies On Bambusa tulda”
I hereby declare that a dissertation work entitled ―Micropropagation studies on Bambusa tulda plant through nodal explant” Submitted to university in fulfillment for the award of degree in Bachelors Of Science (forestry) is carried out by me at State Research Institute Jabalpur Madhya Pradesh.
Poster presentation ptc - kamlesh
This document summarizes plant tissue culture techniques. It discusses the history of plant tissue culture from 1902 to 1987, highlighting important discoveries. The principles of totipotency and plasticity are described. Different types of plant tissue culture are listed, including embryo culture, seed culture, and meristem culture. Applications such as producing virus-free plants, mass propagation, and creating hybrids through protoplast fusion are covered.
Application of plant tissue culture/ micro-propagation
Tissue culture is the process of growing cells or tissues in sterile conditions. It allows for rapid cloning of plant materials. Plant tissue culture involves excising plant parts and growing them on nutrient media. This allows for mass multiplication of plant materials irrespective of season. Some key developments include Haberlandt's proposal of plant cell culture in 1902, and Murashige and Skoog's nutrient medium in 1962. Micropropagation is now used for conservation of rare species, producing disease-free plants, mutation breeding, and more. The future of this technique remains promising.
Plant tissue culture
Plant tissue culture is a technique used to propagate plants asexually under controlled laboratory conditions. It involves culturing plant cells, tissues, or organs on artificial nutrient media. The key principles are totipotency, where plant cells can regenerate into whole plants, and plasticity, where plants can alter their growth to suit their environment. There are various types of plant tissue culture including embryo, seed, meristem, cell, protoplast, callus, pollen, and organ culture. Important applications include producing virus-free plants through meristem culture and generating novel hybrids through protoplast fusion.
Plant tissue culture.rrrr sssss
This document provides an introduction and history of plant tissue culture. It discusses various types of plant tissue cultures including callus culture, cell suspension culture, protoplast culture, meristem culture, anther culture, somatic embryogenesis, and ovary/ovule culture. The key applications of these culture techniques are the production of virus-free plants, mass production of desirable genotypes, production of haploid plants, and genetic transformation.
Prospect of tissue culture in india
1. Introduction: Tissue Culture is the in vitro culture of cells, tissues, organs or whole plant under controlled nutritional and environmental Conditions(T. Thorp, 2007).
The science of plant tissue culture takes its roots from the discovery of Cells (Robert Hooke in 1665) and propounding of cell theory.
In 1838, Schleiden and Schwann proposed that cell is the basic structural unit of all living organisms. They visualized that cell is capable of autonomy and therefore it should be possible for each cell if given an environment to regenerate into whole plants.
2. Plant Tissue Culture: Past & Present Prospects
In 1902, a German physiologist, Gottieb Haberlandt for the first time attempted to culture isolated single palisade cells from leaves in knop’s salt solution.
The cell remained alive for up to 1 month, increased in size, accumulated starch but failed to divide.
Though he was unsuccessful but he laid the foundation of tissue culture so he is regarded as Father of Plant Tissue Culture.
In the Subsequent years different landmark discoveries were made. Some of them are:
Use of specialized media for aseptic culture of Orchid seeds (Knudson, 1925) and other workers also demonstrated that plants could be propagated in vitro from the minuscule seeds of the Orchidaceae.
Further culture of other plant tissue was not possible due to lack of knowledge of the specific hormones to be added to the culture media.
This limitation was overcomed by the elucidation of the nature of Auxin, IAA, by Thimann and Went(1930) that plants would be subsequently regenerated through the use of IAA or its analogs.
Discovery of Cytokinins, specially Kinetin(6-furfurylaminopurine) by Miller et al. (1956), the regeneration of intact plants from tissue of many herbaceous species became a practical reality.
Plant bio 1 introduction to cell tissue culture
Tissue culture is a method of growing plant cells, tissues or organs in vitro on artificial nutrient media under sterile conditions. Plant tissue culture involves exposing plant tissue to specific nutrients, hormones and light to produce many new cloned plants over a short period. The father of plant tissue culture is considered to be German botanist Gottlieb Haberlandt who conceived the concept of cell culture in 1902. A key aspect of plant tissue culture is initiation and maintenance of callus cultures, which are masses of unorganized proliferating cells grown on artificial media.
Plant tissue culture
Plant tissue culture is a collection of techniques used to maintain or grow plant cells, tissues or organs under sterile conditions on a nutrient culture medium of known composition. It is widely used to produce clones of a plant in a method known as micropropagation
Cellular totipotency in plants
The document discusses totipotency in plant cells. Totipotency refers to the ability of single plant cells to regenerate into a whole plant through cell differentiation and tissue culture techniques. The document outlines various tissue culture systems used to study totipotency, including callus culture, suspension culture, single cell culture, and protoplast culture. Factors that influence a cell's ability to express totipotency, such as the explant source and culture conditions, are also discussed.
Ojuederie's college seminar
This document provides an overview of plant tissue culture methods and applications. It discusses the basic concepts of plant tissue culture, including plasticity and totipotency. The stages of micropropagation are outlined as initiation, multiplication, rooting, and transfer to soil. Micropropagation involves regeneration and multiplication of plants from explants like axillary buds and shoot tips. The document also discusses meristem culture, shoot tip culture, growth media, factors affecting tissue culture, applications like disease elimination and germplasm conservation, somaclonal variation, and cryopreservation.
Plant Tissue Culture, Methods and Applications
Introduction to tissue culture, culture types, micropropagation benefits and disadvantages, cryopreservation utilization of plant tissue culture,
Similar to Plant tissuce culture and vegetatively propogated crops f. sarsu (20)
$Tissue culture and advanced crop improvement using biotechnology
$Tissue culture and advanced crop improvement using biotechnology
Introduction to Plant Tissue Culture and Propagation
Introduction to Plant Tissue Culture and Propagation
Tissue Culture its types and Applications By Samra Tahir
Tissue Culture its types and Applications By Samra Tahir
Plant Tissue Culture..“Micropropagation Studies On Bambusa tulda”
Plant Tissue Culture..“Micropropagation Studies On Bambusa tulda”
Application of plant tissue culture/ micro-propagation
Application of plant tissue culture/ micro-propagation
More from Mahbubul Hassan
#1 guidelines for expression of stable isotope ratio results
This document provides guidelines for expressing stable isotope measurement results in a clear and consistent manner. It aims to clarify terminology related to isotope ratios and relative differences in isotope ratios. Key recommendations include using the delta (δ) notation to express relative differences compared to a standard, specifying the isotope when using terms like "depleted" or "enriched", and following International System of Units guidelines for formatting numbers, units, and uncertainty values. The guidelines are intended to improve communication of isotopic data across scientific disciplines.
#1 irms guide finalv3.1 web +++++
This document provides a guide for isotope ratio mass spectrometry (IRMS). It describes the key components and functioning of elemental analyzer IRMS (EA-IRMS) and thermal conversion EA-IRMS systems. These systems involve converting samples to simple gases like CO2, N2, CO, and H2 using an elemental analyzer, then introducing the gases into a mass spectrometer for isotope ratio analysis. The guide outlines instrument setup, calibration, making measurements, data handling procedures, quality assurance, and troubleshooting topics to help users reliably obtain isotope ratio data.
#1 isotope guidelines coplen 2011
This document provides guidelines for expressing stable isotope measurement results in a clear and consistent manner. It aims to clarify terminology related to isotope ratios and relative differences in isotope ratios. Key recommendations include using the delta (δ) notation to express relative differences compared to a reference standard, and specifying the isotope when using terms like "depleted" or "enriched". Measurement results should include associated uncertainties and be reported in a way consistent with international standards for quantities and units. The guidelines are intended to improve communication in scientific fields involving stable isotope measurements.
#1 stable isotope deltas tiny yet robust signatures in nature
This document discusses stable isotope deltas, which are tiny yet robust signatures that can be measured in nature. It explains that two fundamental processes, isotopic fractionation and isotope mixing, are responsible for most stable isotope variations seen in terrestrial systems. Isotopic fractionation occurs through equilibrium or kinetic processes that fractionate isotopes due to small differences in their physical or chemical properties. Isotope mixing models can provide information about processes like 14C abundances in the atmosphere and past ocean isotopic compositions. The document also proposes a new unit called the "urey" to describe isotope deltas in a way that overcomes limitations of traditional units.
#2 determination of o 18 of water and c-13 of dic using simple modification o...
This document describes a method for determining the stable isotope ratios (d18O and d13C) of water and dissolved inorganic carbon using an elemental analyzer coupled to an isotope ratio mass spectrometer. Small amounts of water sample are equilibrated with CO2 gas in sealed vials. The headspace CO2 is then injected into the elemental analyzer for analysis. The method requires only a simple modification to the elemental analyzer and provides precise results without extensive offline sample preparation. Reproducible results with a precision of better than 0.2% can be obtained for both water isotope and dissolved inorganic carbon ratios using this coupled approach.
13 c analyses of calcium carbonate comparison between gb and ea
This document compares the GasBench and elemental analyzer techniques for analyzing the stable carbon isotope composition (d13C) of calcium carbonate samples. It analyzed the d13C of two in-house carbonate standards and ten paleosol samples using both techniques. The results found that for pure calcium carbonate samples, both techniques produced similar d13C values with comparable precision of better than 0.08%. However, the GasBench technique generally had slightly better precision, especially for samples with less than 85% calcium carbonate content. The study suggests the elemental analyzer technique can also be used to analyze the d13C of pure calcium carbonate samples.
13 doc analysis technique - isotope tracer tech inc
Samples are prepared for 13C analysis of dissolved organic carbon by adding phosphoric acid and potassium persulfate to water samples to expel inorganic carbon and digest organic carbon. Samples are then flushed with helium and microwaved to completely release carbon dioxide. Samples are analyzed using continuous flow isotope ratio mass spectrometry where a sample aliquot is injected and analyzed by comparing isotopic ratios to a reference gas. Three internal carbon standards are prepared and analyzed under the same conditions as samples to calibrate results reported against an international reference material.
2013 sk~1
This document reviews normalization procedures and reference material selection for stable isotope analyses. It discusses that normalization methods using linear regression based on two or more reference standards are preferred over single-point normalization or normalization to a working gas. Using multiple reference standards that span the expected range of sample δ values and performing replicate measurements can reduce uncertainty by 50%. While chemical matching between reference materials and samples is important for some materials and techniques, like δ18O of nitrate or δ2H of hair, it is not always necessary. To ensure comparability, laboratories should report details of their normalization procedures and reference materials.
2016 new organic reference materials for h, c, n measurements supporting in...
This document describes 19 new organic reference materials developed for hydrogen, carbon, and nitrogen stable isotope ratio measurements, in addition to analyzing 3 pre-existing reference materials. The new reference materials span a wide range of isotope values and include materials like caffeines, n-alkanes, fatty acid methyl esters, glycines, L-valines, polyethylenes, and oils. Eleven laboratories from 7 countries performed isotope ratio measurements of the materials using multiple analytical techniques. Bayesian statistical analysis was used to determine the mean isotope values for each material. The new reference materials will enable normalization of sample measurements to international isotope scales.
Absolute isotopic scale for deuterium analysis of natural waters
This document defines an absolute isotopic scale for deuterium analysis of natural waters based on measurements of two reference standards - Standard Mean Ocean Water (SMOW) and Standard Light Antarctic Precipitation (SLAP). The absolute D/H ratios were measured through mass spectrometric comparison with calibration mixtures prepared in the laboratory. The results obtained are:
1) The absolute D/H ratio of SMOW is 155.76 ± 0.05 x 10-6.
2) The absolute D/H ratio of SLAP is 89.02 ± 0.05 x 10-6.
3) The δD value of SLAP relative to SMOW is -428.50 ±
Acid fumigation preparing c-13 solid samples for organic analysis
1) The document provides tips for preparing difficult soil, sediment, filter, wood, and carbonate samples for 13C and 15N analysis, including removing inorganic carbonates from calcareous samples.
2) It recommends weighing samples into silver capsules, placing them in an acid desiccator to release carbon dioxide from carbonates over 6-8 hours, then drying and re-encapsulating samples in tin capsules for combustion.
3) The additional tin capsule acts as an important combustion catalyst and prevents leaks that could lose sample material during crimping.
Acid fumigation of soils to remove co3 prior to c 13 isotopic analysis
This document describes a method for removing carbonates from soil samples prior to isotopic analysis of total organic carbon or carbon-13. It compares the effectiveness of acid fumigation using hydrochloric acid vapor versus acid washing. The key findings are:
1) Hydrochloric acid fumigation is highly effective at removing carbonates from soils, does not remove water-soluble organic carbon, and does not alter the carbon-13 signature of the residual soil organic matter.
2) Acid washing soils with hydrochloric acid, while removing carbonates, results in significant losses of total soil carbon and nitrogen as well as changes to the carbon-13 signature.
3) Hydrochloric acid f
Carbonate removal by acid fumigation for measuring 13 c
This study evaluated a method of removing carbonates from soil samples using acid fumigation to allow for accurate measurement of soil organic carbon concentrations and isotopic signatures. Soil samples from two depths were exposed to hydrochloric acid vapors for varying time periods. Analysis found that a minimum of 30 hours of exposure was needed to remove all carbonates from surface soil samples containing 0.80% inorganic carbon, while 56 hours was required for subsurface samples containing 1.94% inorganic carbon. The rate of inorganic carbon removal was similar to previous studies. A correction factor was also used to account for mass changes during fumigation to allow accurate determination of soil organic carbon concentrations.
Carbonate removal from coastal sediments for the determination of organic c a...
The document compares two methods for removing inorganic carbon from samples to isolate organic carbon for analysis: the aqueous method using hydrochloric acid (HClaq) and the vaporous method using hydrochloric acid vapor (HClvap). It evaluates the methods based on their ability to have low blank levels, efficiently remove dolomite, yield accurate measurements of organic carbon percentage and isotopic signatures (δ13C and Δ14C). The vaporous method met all criteria if samples were not overexposed to acid. The aqueous method gave similar results but was less reliable and consistently underestimated organic carbon percentage. Optimal acid exposure times need to be determined for each sample type to obtain the most accurate isotopic measurements.
Forensic application of irms
This document provides an overview of stable isotope ratio mass spectrometry (IRMS) and its forensic applications. IRMS is a technique that can help distinguish between sources of the same substance. It does this by measuring the natural variations in isotope ratios present due to fractionation effects during chemical and physical processes. The document reviews how IRMS has been used to individualize samples in cases involving explosives, ignitable liquids, and illicit drugs. It also discusses the delta notation and standards used to report isotope ratio data and the kinetic and thermodynamic fractionation effects that create characteristic isotope ratio signatures.
Improved method for analysis of dic in natural water samples
This improved method allows for the isotopic and quantitative analysis of dissolved inorganic carbon (DIC) in natural water samples. It involves injecting an aliquot of water into a glass tube containing phosphoric acid, which converts the DIC into gaseous and aqueous carbon dioxide. After 15-24 hours of equilibration, a portion of the headspace gas, mainly carbon dioxide, is introduced into a gas chromatograph coupled to an isotope ratio mass spectrometer to measure the carbon isotope ratio and determine the δ13C value of DIC. Standard solutions are used to calibrate the method and account for carbon isotope fractionation between gaseous and aqueous carbon dioxide phases. The method can analyze around 50 samples per day and
Introduction to isotopic calculation
This document provides an introduction to isotopic calculations, including:
- Methods for expressing isotopic abundances using terms like atom percent and fractional abundance.
- Isotopic mass balance calculations for combinations of materials and isotope dilution analyses.
- The delta notation used to express differences in isotopic composition between samples.
- How fractionations between isotopes can provide information about isotope effects and processes samples have undergone.
- How the reversibility of reactions and whether systems are open or closed impact isotopic distributions between reactants and products at equilibrium.
Isotope ratio mass spectrometry minireview
Isotope ratio mass spectrometry (IRMS) is a technique that determines the relative abundances of isotopes in a sample to find its geographic, chemical, and biological origins. Variations in isotope ratios of elements like carbon, hydrogen, oxygen, sulfur, and nitrogen occur through kinetic and thermodynamic processes and can differentiate between chemically identical samples. IRMS instruments precisely measure subtle differences in natural isotope abundances to provide information in many fields. Sample introduction is usually through elemental analyzers, gas chromatography, or liquid chromatography interfaced with an IRMS instrument.
Measurement of slap2 and gisp 17 o and proposed vsmow slap normalization
The document presents new measurements of the δ17O values of SLAP2 and GISP ice core water samples. It aims to establish a standardized δ17O value for SLAP to improve normalization and reduce discrepancies in reported δ17O and 17Oexcess values between laboratories. The authors measured the samples on a mass spectrometer and recommend defining SLAP to have δ18O = -55.5‰ and 17Oexcess = 0, yielding an approximate δ17O value of -29.6968‰. Using this normalization, their measured values of GISP were δ17O = -13.16 ± 0.05‰ and 17Oexcess = 22 ± 11 per meg. They conclude
Method of sampling and analysis of 13 c dic in groundwaters
This document describes a new method for analyzing the stable carbon isotopic composition (δ13C) of dissolved inorganic carbon (DIC) in groundwater samples. The method uses a gas evolution technique where water samples are injected into vials containing phosphoric acid, which causes the DIC to evolve as CO2 gas. The vials are then analyzed using an automated continuous-flow gas preparation system coupled to an isotope ratio mass spectrometer. This allows for fast (10 minute) analysis of DIC δ13C with high precision (0.1‰) and accuracy. The method is robust, requires minimal field handling, and is well-suited for large sample batches analyzed using an autosampler.
More from Mahbubul Hassan (20)
#1 guidelines for expression of stable isotope ratio results
#1 guidelines for expression of stable isotope ratio results
#1 stable isotope deltas tiny yet robust signatures in nature
#1 stable isotope deltas tiny yet robust signatures in nature
#2 determination of o 18 of water and c-13 of dic using simple modification o...
#2 determination of o 18 of water and c-13 of dic using simple modification o...
13 c analyses of calcium carbonate comparison between gb and ea
13 c analyses of calcium carbonate comparison between gb and ea
13 doc analysis technique - isotope tracer tech inc
13 doc analysis technique - isotope tracer tech inc
2016 new organic reference materials for h, c, n measurements supporting in...
2016 new organic reference materials for h, c, n measurements supporting in...
Absolute isotopic scale for deuterium analysis of natural waters
Absolute isotopic scale for deuterium analysis of natural waters
Acid fumigation preparing c-13 solid samples for organic analysis
Acid fumigation preparing c-13 solid samples for organic analysis
Acid fumigation of soils to remove co3 prior to c 13 isotopic analysis
Acid fumigation of soils to remove co3 prior to c 13 isotopic analysis
Carbonate removal by acid fumigation for measuring 13 c
Carbonate removal by acid fumigation for measuring 13 c
Carbonate removal from coastal sediments for the determination of organic c a...
Carbonate removal from coastal sediments for the determination of organic c a...
Improved method for analysis of dic in natural water samples
Improved method for analysis of dic in natural water samples
Measurement of slap2 and gisp 17 o and proposed vsmow slap normalization
Measurement of slap2 and gisp 17 o and proposed vsmow slap normalization
Method of sampling and analysis of 13 c dic in groundwaters
Method of sampling and analysis of 13 c dic in groundwaters
Recently uploaded
Combined Illegal, Unregulated and Unreported (IUU) Vessel List.
The best available, up-to-date information on all fishing and related vessels that appear on the illegal, unregulated, and unreported (IUU) fishing vessel lists published by Regional Fisheries Management Organisations (RFMOs) and related organisations. The aim of the site is to improve the effectiveness of the original IUU lists as a tool for a wide variety of stakeholders to better understand and combat illegal fishing and broader fisheries crime.
To date, the following regional organisations maintain or share lists of vessels that have been found to carry out or support IUU fishing within their own or adjacent convention areas and/or species of competence:
Commission for the Conservation of Antarctic Marine Living Resources (CCAMLR)
Commission for the Conservation of Southern Bluefin Tuna (CCSBT)
General Fisheries Commission for the Mediterranean (GFCM)
Inter-American Tropical Tuna Commission (IATTC)
International Commission for the Conservation of Atlantic Tunas (ICCAT)
Indian Ocean Tuna Commission (IOTC)
Northwest Atlantic Fisheries Organisation (NAFO)
North East Atlantic Fisheries Commission (NEAFC)
North Pacific Fisheries Commission (NPFC)
South East Atlantic Fisheries Organisation (SEAFO)
South Pacific Regional Fisheries Management Organisation (SPRFMO)
Southern Indian Ocean Fisheries Agreement (SIOFA)
Western and Central Pacific Fisheries Commission (WCPFC)
The Combined IUU Fishing Vessel List merges all these sources into one list that provides a single reference point to identify whether a vessel is currently IUU listed. Vessels that have been IUU listed in the past and subsequently delisted (for example because of a change in ownership, or because the vessel is no longer in service) are also retained on the site, so that the site contains a full historic record of IUU listed fishing vessels.
Unlike the IUU lists published on individual RFMO websites, which may update vessel details infrequently or not at all, the Combined IUU Fishing Vessel List is kept up to date with the best available information regarding changes to vessel identity, flag state, ownership, location, and operations.
Awaken new depths - World Ocean Day 2024, June 8th.
UN WOD 2024 will take us on a journey of discovery through the ocean's vastness, tapping into the wisdom and expertise of global policy-makers, scientists, managers, thought leaders, and artists to awaken new depths of understanding, compassion, collaboration and commitment for the ocean and all it sustains. The program will expand our perspectives and appreciation for our blue planet, build new foundations for our relationship to the ocean, and ignite a wave of action toward necessary change.
How To Cultivate Community Affinity Throughout The Generosity Journey
This session will dive into how to create rich generosity experiences that foster long-lasting relationships. You’ll walk away with actionable insights to redefine how you engage with your supporters — emphasizing trust, engagement, and community!
IEA World Energy Investment June 2024- Statistics
IEA World Energy Investment Report 2024
Publication: June 2024
在线办理(ISU毕业证书)爱荷华州立大学毕业证学历证书一模一样
学校原件一模一样【微信：741003700 】《(ISU毕业证书)爱荷华州立大学毕业证学历证书》【微信：741003700 】学位证，留信认证（真实可查，永久存档）原件一模一样纸张工艺/offer、雅思、外壳等材料/诚信可靠,可直接看成品样本，帮您解决无法毕业带来的各种难题！外壳，原版制作，诚信可靠，可直接看成品样本。行业标杆！精益求精，诚心合作，真诚制作！多年品质 ,按需精细制作，24小时接单,全套进口原装设备。十五年致力于帮助留学生解决难题，包您满意。
本公司拥有海外各大学样板无数，能完美还原。
1:1完美还原海外各大学毕业材料上的工艺：水印，阴影底纹，钢印LOGO烫金烫银，LOGO烫金烫银复合重叠。文字图案浮雕、激光镭射、紫外荧光、温感、复印防伪等防伪工艺。材料咨询办理、认证咨询办理请加学历顾问Q/微741003700
【主营项目】
一.毕业证【q微741003700】成绩单、使馆认证、教育部认证、雅思托福成绩单、学生卡等！
二.真实使馆公证(即留学回国人员证明,不成功不收费)
三.真实教育部学历学位认证（教育部存档！教育部留服网站永久可查）
四.办理各国各大学文凭(一对一专业服务,可全程监控跟踪进度)
如果您处于以下几种情况：
◇在校期间，因各种原因未能顺利毕业……拿不到官方毕业证【q/微741003700】
◇面对父母的压力，希望尽快拿到；
◇不清楚认证流程以及材料该如何准备；
◇回国时间很长，忘记办理；
◇回国马上就要找工作，办给用人单位看；
◇企事业单位必须要求办理的
◇需要报考公务员、购买免税车、落转户口
◇申请留学生创业基金
留信网认证的作用:
1:该专业认证可证明留学生真实身份
2:同时对留学生所学专业登记给予评定
3:国家专业人才认证中心颁发入库证书
4:这个认证书并且可以归档倒地方
5:凡事获得留信网入网的信息将会逐步更新到个人身份内，将在公安局网内查询个人身份证信息后，同步读取人才网入库信息
6:个人职称评审加20分
7:个人信誉贷款加10分
8:在国家人才网主办的国家网络招聘大会中纳入资料，供国家高端企业选择人才
World Food Safety Day 2024- Communication-toolkit.
Food safety, prepare for the unexpected - So what can be done in order to be ready to address food safety, food Consumers, food producers and manufacturers, food transporters, food businesses, food retailers can ...
United Nations World Oceans Day 2024; June 8th " Awaken new dephts".
The program will expand our perspectives and appreciation for our blue planet, build new foundations for our relationship to the ocean, and ignite a wave of action toward necessary change.
快速办理(UVM毕业证书)佛蒙特大学毕业证学位证一模一样
学校原件一模一样【微信：741003700 】《(UVM毕业证书)佛蒙特大学毕业证学位证》【微信：741003700 】学位证，留信认证（真实可查，永久存档）原件一模一样纸张工艺/offer、雅思、外壳等材料/诚信可靠,可直接看成品样本，帮您解决无法毕业带来的各种难题！外壳，原版制作，诚信可靠，可直接看成品样本。行业标杆！精益求精，诚心合作，真诚制作！多年品质 ,按需精细制作，24小时接单,全套进口原装设备。十五年致力于帮助留学生解决难题，包您满意。
本公司拥有海外各大学样板无数，能完美还原。
1:1完美还原海外各大学毕业材料上的工艺：水印，阴影底纹，钢印LOGO烫金烫银，LOGO烫金烫银复合重叠。文字图案浮雕、激光镭射、紫外荧光、温感、复印防伪等防伪工艺。材料咨询办理、认证咨询办理请加学历顾问Q/微741003700
【主营项目】
一.毕业证【q微741003700】成绩单、使馆认证、教育部认证、雅思托福成绩单、学生卡等！
二.真实使馆公证(即留学回国人员证明,不成功不收费)
三.真实教育部学历学位认证（教育部存档！教育部留服网站永久可查）
四.办理各国各大学文凭(一对一专业服务,可全程监控跟踪进度)
如果您处于以下几种情况：
◇在校期间，因各种原因未能顺利毕业……拿不到官方毕业证【q/微741003700】
◇面对父母的压力，希望尽快拿到；
◇不清楚认证流程以及材料该如何准备；
◇回国时间很长，忘记办理；
◇回国马上就要找工作，办给用人单位看；
◇企事业单位必须要求办理的
◇需要报考公务员、购买免税车、落转户口
◇申请留学生创业基金
留信网认证的作用:
1:该专业认证可证明留学生真实身份
2:同时对留学生所学专业登记给予评定
3:国家专业人才认证中心颁发入库证书
4:这个认证书并且可以归档倒地方
5:凡事获得留信网入网的信息将会逐步更新到个人身份内，将在公安局网内查询个人身份证信息后，同步读取人才网入库信息
6:个人职称评审加20分
7:个人信誉贷款加10分
8:在国家人才网主办的国家网络招聘大会中纳入资料，供国家高端企业选择人才
RFP for Reno's Community Assistance Center
Property appraisals completed in May for downtown Reno’s Community Assistance and Triage Centers (CAC) reveal that repairing the buildings to bring them back into service would cost an estimated $10.1 million—nearly four times the amount previously reported by city staff.
Indira awas yojana housing scheme renamed as PMAY
Indira Awas Yojana (IAY) played a significant role in addressing rural housing needs in India. It emerged as a comprehensive program for affordable housing solutions in rural areas, predating the government’s broader focus on mass housing initiatives.
Milton Keynes Hospital Charity - A guide to leaving a gift in your Will
A forever thank you in your Will to Milton Keynes University Hospital can change lives for years to come.
AHMR volume 10 number 1 January-April 2024
AHMR is an interdisciplinary peer-reviewed online journal created to encourage and facilitate the study of all aspects (socio-economic, political, legislative and developmental) of Human Mobility in Africa. Through the publication of original research, policy discussions and evidence research papers AHMR provides a comprehensive forum devoted exclusively to the analysis of contemporaneous trends, migration patterns and some of the most important migration-related issues.
Contributi dei parlamentari del PD - Contributi L. 3/2019
DI SEGUITO SONO PUBBLICATI, AI SENSI DELL'ART. 11 DELLA LEGGE N. 3/2019, GLI IMPORTI RICEVUTI DALL'ENTRATA IN VIGORE DELLA SUDDETTA NORMA (31/01/2019) E FINO AL MESE SOLARE ANTECEDENTE QUELLO DELLA PUBBLICAZIONE SUL PRESENTE SITO
在线办理美国乔治华盛顿大学毕业证（gwu毕业证书）学历学位证书原版一模一样
原版一模一样【微信：741003700 】【美国乔治华盛顿大学毕业证（gwu毕业证书）学历学位证书】【微信：741003700 】学位证，留信认证（真实可查，永久存档）offer、雅思、外壳等材料/诚信可靠,可直接看成品样本，帮您解决无法毕业带来的各种难题！外壳，原版制作，诚信可靠，可直接看成品样本。行业标杆！精益求精，诚心合作，真诚制作！多年品质 ,按需精细制作，24小时接单,全套进口原装设备。十五年致力于帮助留学生解决难题，包您满意。
本公司拥有海外各大学样板无数，能完美还原海外各大学 Bachelor Diploma degree, Master Degree Diploma
1:1完美还原海外各大学毕业材料上的工艺：水印，阴影底纹，钢印LOGO烫金烫银，LOGO烫金烫银复合重叠。文字图案浮雕、激光镭射、紫外荧光、温感、复印防伪等防伪工艺。材料咨询办理、认证咨询办理请加学历顾问Q/微741003700
留信网认证的作用:
1:该专业认证可证明留学生真实身份
2:同时对留学生所学专业登记给予评定
3:国家专业人才认证中心颁发入库证书
4:这个认证书并且可以归档倒地方
5:凡事获得留信网入网的信息将会逐步更新到个人身份内，将在公安局网内查询个人身份证信息后，同步读取人才网入库信息
6:个人职称评审加20分
7:个人信誉贷款加10分
8:在国家人才网主办的国家网络招聘大会中纳入资料，供国家高端企业选择人才
Bangladesh studies presentation on Liberation War 1971 Indepence-of-Banglades...
Bangladesh studies presentation on Liberation War 1971
Recently uploaded (20)
Combined Illegal, Unregulated and Unreported (IUU) Vessel List.
Combined Illegal, Unregulated and Unreported (IUU) Vessel List.
Awaken new depths - World Ocean Day 2024, June 8th.
Awaken new depths - World Ocean Day 2024, June 8th.
How To Cultivate Community Affinity Throughout The Generosity Journey
How To Cultivate Community Affinity Throughout The Generosity Journey
World Food Safety Day 2024- Communication-toolkit.
World Food Safety Day 2024- Communication-toolkit.
United Nations World Oceans Day 2024; June 8th " Awaken new dephts".
United Nations World Oceans Day 2024; June 8th " Awaken new dephts".
Researching the client.pptxsxssssssssssssssssssssss
Researching the client.pptxsxssssssssssssssssssssss
Milton Keynes Hospital Charity - A guide to leaving a gift in your Will
Milton Keynes Hospital Charity - A guide to leaving a gift in your Will
Contributi dei parlamentari del PD - Contributi L. 3/2019
Contributi dei parlamentari del PD - Contributi L. 3/2019
Bangladesh studies presentation on Liberation War 1971 Indepence-of-Banglades...
Bangladesh studies presentation on Liberation War 1971 Indepence-of-Banglades...
Plant tissuce culture and vegetatively propogated crops f. sarsu
- 1. Atoms for Food and Agriculture: Meeting the Challenge Plant Tissue Culture Techniques for the use of Mutation Induction in Plant Breeding Fatma SARSU Technical Officer Joint FAO/IAEA Programme of Nuclear Techniquesin Food and Agriculture
- 2. Outline plant tissue culture: quick review Mutationinduction: quick review Application of mutationinduction on in vitro cultures
- 3. Plant Tissue Culture? Leaf explants Immature cotyledon 30 days after initiation weeks after after initiation A technique of growing in vitro all plant parts whether a single cell , a tissue or an organ on synthetic medium under controlledand aseptic conditions Totipotencytheory states that cells are autonomicand in principle are capable of regenerating to give a completeplant Plant cell totipotency ! Cotyledon 4
- 4. History of plant tissue culture Cellular theory (Cell is autonom and totipotent, the basic unit of life) Schleiden-Schwann 1838-39 First attempts to cultivate isolated plant cell in vitro on a nutrient medium –Harberlandt 1902 White (1934) continuouslygrowing callus culture MS media- (Murashige and Skoog, 1962) Haploids from pollen grains of Datura –Guha and Maheswari,1964 Fusion of protoplasts-Power1970 Plants from tobacco protoplasts-Takebe 1971 Somaclonal variation –Larkin and Scowcroft-1981 PTC applications have been widely developed in last few decades in worlwide
- 5. Plant Tissue Culture Facilities(1) A general washing area A media preparation, sterilization and storage area (incubators, centrifuges, autoclaves…) An aseptic transfer area ( air flow cabinet…)