This document describes a method for removing carbonates from soil samples prior to isotopic analysis of total organic carbon or carbon-13. It compares the effectiveness of acid fumigation using hydrochloric acid vapor versus acid washing. The key findings are:
1) Hydrochloric acid fumigation is highly effective at removing carbonates from soils, does not remove water-soluble organic carbon, and does not alter the carbon-13 signature of the residual soil organic matter.
2) Acid washing soils with hydrochloric acid, while removing carbonates, results in significant losses of total soil carbon and nitrogen as well as changes to the carbon-13 signature.
3) Hydrochloric acid f
A STUDY ON OCEAN ACIDIFICATION DUE TO CARBON DIOXIDE ALONG THE COAST OF VISAK...Soma Sekhar Sriadibhatla
Extensive Data Analytics on samples to understand Ocean Acidification process, a serious damage to ecosystem, increase in production of Carbon dioxide.
Hydrogeochemistry and Microbiology of Wadi Al Bih Limestone Aquifer in Northe...QUESTJOURNAL
ABSTRACT: This study investigated the hydrogeochemical characteristics and microbiological pollution of groundwater in Wadi Al Bih limestone aquifer, Ras Al Khaimah area, northern United Arab Emirates (UAE). Results indicate that the decrease of groundwater exploitation in Wadi Al Bih basin from 58 million cubic meter (MCM) during the period 1991-1995 to 22 MCM during the period 2011-2015, has increased groundwater storage, raised hydraulic heads by 1 m in Al Burayrat area and 16 m near Wadi Al Bih main dam, and decreased the average groundwater salinity by 30% in Wadi Al Bih well field and 45% in Al Burayrat well field. Results of chemical analyses showed noticeable fluctuations in groundwater temperature, EC, and TDS contents, rather than concentrations of all ions. The November 2014 and June 2015 isosalinity contour maps indicate that the groundwater salinity increases from east to west, in the direction of groundwater flow. The groundwater in the eastern part of Wadi Al Bih is good for domestic purposes and irrigation. However, the water hardness is high because the aquifer is predominantly composed of limestone. Wadi Al Bih limestone aquifer is highly sensitive to urban and agricultural activities, and several well were recorded to have Coliform bacteria in Wadi Al Bih and Al Burayrat areas
A STUDY ON OCEAN ACIDIFICATION DUE TO CARBON DIOXIDE ALONG THE COAST OF VISAK...Soma Sekhar Sriadibhatla
Extensive Data Analytics on samples to understand Ocean Acidification process, a serious damage to ecosystem, increase in production of Carbon dioxide.
Hydrogeochemistry and Microbiology of Wadi Al Bih Limestone Aquifer in Northe...QUESTJOURNAL
ABSTRACT: This study investigated the hydrogeochemical characteristics and microbiological pollution of groundwater in Wadi Al Bih limestone aquifer, Ras Al Khaimah area, northern United Arab Emirates (UAE). Results indicate that the decrease of groundwater exploitation in Wadi Al Bih basin from 58 million cubic meter (MCM) during the period 1991-1995 to 22 MCM during the period 2011-2015, has increased groundwater storage, raised hydraulic heads by 1 m in Al Burayrat area and 16 m near Wadi Al Bih main dam, and decreased the average groundwater salinity by 30% in Wadi Al Bih well field and 45% in Al Burayrat well field. Results of chemical analyses showed noticeable fluctuations in groundwater temperature, EC, and TDS contents, rather than concentrations of all ions. The November 2014 and June 2015 isosalinity contour maps indicate that the groundwater salinity increases from east to west, in the direction of groundwater flow. The groundwater in the eastern part of Wadi Al Bih is good for domestic purposes and irrigation. However, the water hardness is high because the aquifer is predominantly composed of limestone. Wadi Al Bih limestone aquifer is highly sensitive to urban and agricultural activities, and several well were recorded to have Coliform bacteria in Wadi Al Bih and Al Burayrat areas
Mineralization of Carbon from Sewage sludge in three soils of the Argentine p...Silvana Torri
Como citar este trabajo
Torri S, Alvarez R, Lavado R. 2003. Mineralization of Carbon from Sewage sludge in three soils of the Argentine pampas. Commun. Soil Sci. and Plant Anal. (Taylor & Francis, Inc., 325 Chestnut Street, Suite 800, Philadelphia, PA 19106) 34 (13-14): 2035-2043. ISSN (impresa): 0010-3624. ISSN (electronica): 1532-2416.
An arsenic corrosion inhibitor named W-41 was used at Lake St. John oilfield in the 1950s. The geochemical signature of the arsenic usage and its migration is preserved in shallow groundwater below the old oilfield pits.
First lab managers’ meeting of the South-East Asia Laboratory NETwork (SEALNET 2.0) - Quality improvement in Asian soil laboratories: towards standardization and harmonization of soil analyses and their interpretation, Bogor, Indonesia, 20 - 24 November 2017.
General principles of soil test interpretation ExternalEvents
First lab managers’ meeting of the South-East Asia Laboratory NETwork (SEALNET 2.0) - Quality improvement in Asian soil laboratories: towards standardization and harmonization of soil analyses and their interpretation, Bogor, Indonesia, 20 - 24 November 2017.
a reductive dissolution model to explain shallow groundwater arsenic was used in Lake St. John Field by Geosyntec (2008). However, public records indicate arsenic-based corrosion inhibitors were used in the field. Extensive publically-available geochemical data indicate the model does not explain the pit-related groundwater arsenic.
The Effect of Surface Temperature and Salinity of Ocean Water on Carbon Dioxi...Vignesh Rajmohan
The varying temperatures and salinities of ocean water from around the world might play a role in the amount of CO2 that can be absorbed by that part of the ocean. If patterns could be found to those amounts and the carbon dioxide dissolution limit, then one could create a diagram of the world’s oceans and display where the most carbon dioxide could be absorbed, which also leads to where the most damage will be caused because of the maximum amount of ocean acidification. To test this question, solutions with various salinities (28, 34 and 38 ppt) were prepared. Dry ice was added to the solutions to find the maximum CO2 dissolution by identifying or measuring the pH using the pH indicator. Then the acidic solution was neutralized using baking soda and weighed to quantify the amount of baking soda dissolved. Then those steps are repeated at these temperatures (40, 57, 70 and 80 F) for each salinity to understand the dependency of temperature. Then using the data, plots are made to locate the maximum acidification by carbon dioxide in the oceans of the world. In general, the results stated that as salinity rises, the amount of carbon dioxide that can be absorbed by the ocean rises. As the temperature reaches a medium temperature at around 60 degrees Fahrenheit, the ocean water reaches its highest absorption potential. As temperatures get farther away from about 60 degrees Fahrenheit, the carbon dioxide absorption potential decreases.
Determination potassium by_ammonium_acetate_extraction_method_zahid_sau_sylhetSyed Zahid Hasan
Determination_potassium_by_ammonium_acetate_extraction_method_zahid_sau_sylhet.
Some picture and data are collected from internet. procedure is in short form so that it can understand easily.
There is no shortcut of success.
Read book first.
Objectives:
- Determine how soil moisture and nutrients regulate microbial C-use efficiency (CUE)
- Develop mathematical functions that can be incorporated into earth system models
- Improve our ability to predict the impact of climate change on soil C-sequestration in agricultural systems
Mineralization of Carbon from Sewage sludge in three soils of the Argentine p...Silvana Torri
Como citar este trabajo
Torri S, Alvarez R, Lavado R. 2003. Mineralization of Carbon from Sewage sludge in three soils of the Argentine pampas. Commun. Soil Sci. and Plant Anal. (Taylor & Francis, Inc., 325 Chestnut Street, Suite 800, Philadelphia, PA 19106) 34 (13-14): 2035-2043. ISSN (impresa): 0010-3624. ISSN (electronica): 1532-2416.
An arsenic corrosion inhibitor named W-41 was used at Lake St. John oilfield in the 1950s. The geochemical signature of the arsenic usage and its migration is preserved in shallow groundwater below the old oilfield pits.
First lab managers’ meeting of the South-East Asia Laboratory NETwork (SEALNET 2.0) - Quality improvement in Asian soil laboratories: towards standardization and harmonization of soil analyses and their interpretation, Bogor, Indonesia, 20 - 24 November 2017.
General principles of soil test interpretation ExternalEvents
First lab managers’ meeting of the South-East Asia Laboratory NETwork (SEALNET 2.0) - Quality improvement in Asian soil laboratories: towards standardization and harmonization of soil analyses and their interpretation, Bogor, Indonesia, 20 - 24 November 2017.
a reductive dissolution model to explain shallow groundwater arsenic was used in Lake St. John Field by Geosyntec (2008). However, public records indicate arsenic-based corrosion inhibitors were used in the field. Extensive publically-available geochemical data indicate the model does not explain the pit-related groundwater arsenic.
The Effect of Surface Temperature and Salinity of Ocean Water on Carbon Dioxi...Vignesh Rajmohan
The varying temperatures and salinities of ocean water from around the world might play a role in the amount of CO2 that can be absorbed by that part of the ocean. If patterns could be found to those amounts and the carbon dioxide dissolution limit, then one could create a diagram of the world’s oceans and display where the most carbon dioxide could be absorbed, which also leads to where the most damage will be caused because of the maximum amount of ocean acidification. To test this question, solutions with various salinities (28, 34 and 38 ppt) were prepared. Dry ice was added to the solutions to find the maximum CO2 dissolution by identifying or measuring the pH using the pH indicator. Then the acidic solution was neutralized using baking soda and weighed to quantify the amount of baking soda dissolved. Then those steps are repeated at these temperatures (40, 57, 70 and 80 F) for each salinity to understand the dependency of temperature. Then using the data, plots are made to locate the maximum acidification by carbon dioxide in the oceans of the world. In general, the results stated that as salinity rises, the amount of carbon dioxide that can be absorbed by the ocean rises. As the temperature reaches a medium temperature at around 60 degrees Fahrenheit, the ocean water reaches its highest absorption potential. As temperatures get farther away from about 60 degrees Fahrenheit, the carbon dioxide absorption potential decreases.
Determination potassium by_ammonium_acetate_extraction_method_zahid_sau_sylhetSyed Zahid Hasan
Determination_potassium_by_ammonium_acetate_extraction_method_zahid_sau_sylhet.
Some picture and data are collected from internet. procedure is in short form so that it can understand easily.
There is no shortcut of success.
Read book first.
Objectives:
- Determine how soil moisture and nutrients regulate microbial C-use efficiency (CUE)
- Develop mathematical functions that can be incorporated into earth system models
- Improve our ability to predict the impact of climate change on soil C-sequestration in agricultural systems
Measurement of soil carbon is the focus of attention of present and future international conventions and agreements, related to global climate change. Past inventories and current carbon stock inventories involve different analytical methods, and methodological biases and uncertainties should be reduced to develop reliable estimates of the effects of land uses changes on total organic carbon. Furthermore, the carbon-equivalent is highly variable, and there is the need of using a specific correction factor for each location, resulting from the combination of land use, textural gradients, and sampling depth. In this context, the aims of this study were creating correction equations for the determinations through wet combustion (Walkley-Black- WB) for a Rhodic Hapludox based on the determinations made through dry combustion (CS) at different depths and management systems. The experimental design was 4 x 5 factorial with 3 replications. Treatments were: Conventional Tillage (CT); Minimum Tillage (MT); No-till with chisel plowing (NTC) and No-Till (NT). The collection depths were: 0-2.5; 2.5-5; 5-10; 10-20 and 20-40 cm. The measured carbon equivalent values ranged from 1.06 to 1.18 and were dependent on land use and soil depth. Rhodic Hapludox under different management presented the following order of carbon equivalent values: NTC < CT < NT < MT. The carbon equivalent values increased with depth. The high ratio between C-WB and C-CS (R2= 0.75, p= 0.0001) justifies the use of correction factors.
Using Carbon Isotopes to Monitor CO2 at the CONSOL Energy Inc. Coal Sequestr...Stephen Henry
The use of carbon isotopes is one of the most effective ways of monitoring, verifying and accounting (MVA) for injected CO2 as carbon forms part of the CO2 molecule itself. This study focuses on using carbon isotopes to understand CO2 dissolution, monitor CO2 plume migration, and identify the presence/absence of CO2 leakage into overlying formations and shallow subsurface at the CONSOL Energy Inc. sequestration test site located in Marshall County, West Virginia along the Pennsylvania Fork of Fish Creek. The CO2 gas is being injected, for testing both coal bed methane recovery and carbon sequestration, into the Upper Freeport coal bed at ~1200 feet depth. Ongoing CO2 injection began in 2009, and continuous geochemical environmental monitoring of shallow ground water, Fish Creek, and vadose zone gas began in 2008. In summer 2012 water and gas sampling began for carbon isotopic analysis at or near the test site; water is being sampled and tested from three groundwater monitoring wells, a few domestic groundwater supplies, and Fish Creek. Gas is being sampled and tested from the CO2 injection source gas tank, two deep coal bed methane producing wells, one deep monitoring well, and eight shallow vadose zone monitoring wells. The preliminary data indicate that the CO2 gas used in this sequestration operation has a different isotopic value compared to naturally occurring CO2 in the geologic formations. Initial data indicate that carbon isotopes can be used as an effective “natural built-in tracer” for monitoring the CO2 plume and/or its leakage into overlying shallow aquifers and subsurface.
Soil Organic Carbon stabilization in compost amended soilsExternalEvents
This presentation was presented during the 2 Parallel session on Theme 2, Maintaining and/or increasing SOC stocks for climate change mitigation and adaptation and Land Degradation Neutrality, of the Global Symposium on Soil Organic Carbon that took place in Rome 21-23 March 2017. The presentation was made by Mr. Riccardo Spaccini, from Universitá di Napoli Federico II - Italy, in FAO Hq, Rome
Stable isotope methodology can be employed in the study and characterisation of soil carbonates on the basis of their reaction time during the acidification step.
Jennifer Schaus and Associates hosts a complimentary webinar series on The FAR in 2024. Join the webinars on Wednesdays and Fridays at noon, eastern.
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Russian anarchist and anti-war movement in the third year of full-scale warAntti Rautiainen
Anarchist group ANA Regensburg hosted my online-presentation on 16th of May 2024, in which I discussed tactics of anti-war activism in Russia, and reasons why the anti-war movement has not been able to make an impact to change the course of events yet. Cases of anarchists repressed for anti-war activities are presented, as well as strategies of support for political prisoners, and modest successes in supporting their struggles.
Thumbnail picture is by MediaZona, you may read their report on anti-war arson attacks in Russia here: https://en.zona.media/article/2022/10/13/burn-map
Links:
Autonomous Action
http://Avtonom.org
Anarchist Black Cross Moscow
http://Avtonom.org/abc
Solidarity Zone
https://t.me/solidarity_zone
Memorial
https://memopzk.org/, https://t.me/pzk_memorial
OVD-Info
https://en.ovdinfo.org/antiwar-ovd-info-guide
RosUznik
https://rosuznik.org/
Uznik Online
http://uznikonline.tilda.ws/
Russian Reader
https://therussianreader.com/
ABC Irkutsk
https://abc38.noblogs.org/
Send mail to prisoners from abroad:
http://Prisonmail.online
YouTube: https://youtu.be/c5nSOdU48O8
Spotify: https://podcasters.spotify.com/pod/show/libertarianlifecoach/episodes/Russian-anarchist-and-anti-war-movement-in-the-third-year-of-full-scale-war-e2k8ai4
ZGB - The Role of Generative AI in Government transformation.pdfSaeed Al Dhaheri
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Understanding the Challenges of Street ChildrenSERUDS INDIA
By raising awareness, providing support, advocating for change, and offering assistance to children in need, individuals can play a crucial role in improving the lives of street children and helping them realize their full potential
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This session provides a comprehensive overview of the latest updates to the Uniform Administrative Requirements, Cost Principles, and Audit Requirements for Federal Awards (commonly known as the Uniform Guidance) outlined in the 2 CFR 200.
With a focus on the 2024 revisions issued by the Office of Management and Budget (OMB), participants will gain insight into the key changes affecting federal grant recipients. The session will delve into critical regulatory updates, providing attendees with the knowledge and tools necessary to navigate and comply with the evolving landscape of federal grant management.
Learning Objectives:
- Understand the rationale behind the 2024 updates to the Uniform Guidance outlined in 2 CFR 200, and their implications for federal grant recipients.
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A process server is a authorized person for delivering legal documents, such as summons, complaints, subpoenas, and other court papers, to peoples involved in legal proceedings.
Presentation by Jared Jageler, David Adler, Noelia Duchovny, and Evan Herrnstadt, analysts in CBO’s Microeconomic Studies and Health Analysis Divisions, at the Association of Environmental and Resource Economists Summer Conference.
Jennifer Schaus and Associates hosts a complimentary webinar series on The FAR in 2024. Join the webinars on Wednesdays and Fridays at noon, eastern.
Recordings are on YouTube and the company website.
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Acid fumigation of soils to remove co3 prior to c 13 isotopic analysis
1. DIVISION S-8—NOTES
pathway of vegetation (C3 vs. C4). When a sample is
ACID FUMIGATION OF SOILS TO
combusted at high temperature (1000⬚C), all organic
REMOVE CARBONATES PRIOR TO and inorganic C present in the soil is converted into
CO2. To avoid the confounding influence of inorganic
TOTAL ORGANIC CARBON
C during the determination of the isotopic signature of
OR CARBON-13 ISOTOPIC ANALYSIS
the organic C, all carbonates must be removed prior to
isotopic analysis. Because carbonates may be enriched
David Harris, William R. Horwáth,
in 13
C by as much as 30‰ compared with organic C,
and Chris van Kessel*
partial removal of carbonates will have a large effect
on the ␦13
C signature of the sample. For example, if
Abstract
residual carbonate at 0‰ accounts for 1% of total soil
The use of 13
C natural abundance (␦13
C) to follow C input to soil
C in a soil where organic C is ⫺25‰, the inclusion of the
has gained widespread acceptance. However, inorganic C present in
CO2 in the measurement would result in a 0.25‰ error.
the soil as carbonates will interfere with the measurement of soil
A common method to remove carbonates from soil
organic 13
C unless removed or excluded from measurement. We report
is treatment with dilute HCl or H3PO4 (Connin et al.,
a simple and convenient HCl-fumigation method to remove inorganic
1997; Rochette and Flanagan, 1997; Collins et al., 1999;
C from soil. Soil samples are weighed in Ag-foil capsules, arranged
on a microtiter plate, wetted with water to approximately field capac- Van Kessel et al., 2000b). Although acid washing will
ity, and placed in a desiccator containing a beaker with concentrated remove all carbonates, the procedure is time consuming
(12 M) HCl. The carbonates are released as CO2 by the acid treatment and could lead to losses of acid-soluble organic C. Thus,
in 6 to 8 h. The soil samples are then dried at 60ⴗC prior to isotope there is a risk that the soluble C may be isotopically
determination. The advantages of the HCl-fumigation method to re-
different from the insoluble residue. However, Mid-
move inorganic C from the soil are that: (i) no water soluble C will
wood and Boutton (1998) found that the ␦13
C signature
be lost from the soil; (ii) a large number of samples can be processed
of SOM C was largely unaffected by the acid concentra-
simultaneously; (iii) the removal of inorganic C is rapid and complete;
tion (0.1–6 M HCl) or duration (1–8 d) of the acid
and (iv) the method could also be used to determine both organic
treatment. To avoid losses of soluble organic C and
and inorganic C content in the soil. A potential disadvantage, however,
possible changes in the ␦13
C signature of SOM, the re-
is that the HCl fumigation changed the 15
N natural abundance of soil N.
moval of carbonates can be carried out by HCl fumiga-
tion (Hedges and Stern, 1984).
Using continuous flow isotope ratio mass spectrome-
The ␦ 13
C method is increasingly being used to follow
ters, it has been common to determine both the 13
C and
C dynamics in the soil (Balesdent and Mariotti,
15
N isotopes from a single combusted sample (Nadelhof-
1996; Collins et al., 1999). The difference between the
fer and Fry, 1988; Van Kessel et al., 1994). However, the
13
C content of the existing soil organic matter (SOM)
possibility that HCl fumigation changes the 15
N isotopic
and the new plant C may result from changes in the pho-
composition of the sample must be investigated if C and
tosynthetic pathway of vegetation (C3 vs. C4) (Bales-
N isotope measurements are to be routinely applied.
dent and Mariotti, 1996; Collins et al., 1999), or when
The main objectives of this study were (i) to deter-
13
C depleted CO2 is used to elevate the atmospheric CO2
mine the effectiveness of HCl fumigation in removing
concentration such as in a free atmospheric CO2 en-
carbonates from soil, (ii) to test the effects of HCl treat-
richment (FACE) experiment (Leavitt et al., 1994; Van
ments on the residual SOM 13
C, and (iii) to determine
Kessel et al., 2000a,b). The difference in the ␦13
C values
the effects of the HCl treatment on ␦15
N of soil N. The
of the new C input and of the older SOM C is sufficiently
possible use of the HCl-fumigation method to determine
large to follow the dynamics of both the new and the
inorganic and organic C contents was also explored.
old C in the soil (Balesdent et al., 1988).
In addition to organic C, soil may also contain inor-
Materials and Methods
ganic C in the form of carbonates. The C in primary or
lithogenic carbonates has ␦13
C values close to 0‰ Pee To determine the rate and effectiveness of carbonate re-
Dee Belemnite (PDB) (Boutton, 1991). Pedogenic or moval by HCl vapor, a calcareous soil with a high inorganic
C content was selected (Table 1). The calcareous soil (Molli-
secondary carbonates show ␦13
C values between ⫺12
sol) was oven-dried and ball-milled for 24 h. Subsamples (30
and ⫹2‰ (PDB), depending on the photosynthetic
mg) of soil were placed in open Ag-foil capsules (8 by 5 mm).
Silver capsules are required because Sn capsules disintegrate
D. Harris, Stable Isotope Lab., Univ. of California-Davis, Davis, CA
95616; W.R. Horwáth, Dep. of Land, Air, and Water Resources, when exposed to HCl vapor. The capsules were placed in the
Univ. of California-Davis, Davis, CA 95616; C. van Kessel, Dep. of wells of a microtiter plate, sufficient water was added to each
Agronomy and Range Science, Univ. of California-Davis, Davis, CA capsule (50 L) to moisten the soil to approximately field
95616. Received 13 July 2000. *Corresponding author (cvankessel@
ucdavis.edu).
Abbreviations: SOM, soil organic matter; FACE, Free Atmospheric
CO2 Enrichment; HCl, HCl acid; ␦ 13
C, natural abundance 13
C.
Published in Soil Sci. Soc. Am. J. 65:1853–1856 (2001).
1853
2. 1854 SOIL SCI. SOC. AM. J., VOL. 65, NOVEMBER–DECEMBER 2001
Table 1. Physical and chemical characteristics of the soils used for the HCl fumigation and washing experiment.
Soil order and description Common name Horizon pH Total C Total N Sand Silt Clay CEC
mg g⫺1
g kg⫺1
cmol kg⫺1
Inceptisol: loamy, mixed, superactive,
thermic Lithic Haploxerepts Auburn A† 5.8 29.1 2.5 460 320 120 12.2‡
Inceptisol: loamy, mixed, active,
mesic shallow, Typic Dystroxerepts Chawanakee A† 5.8 24.8 1.3 180 90 630 6.4‡
Inceptisol: loamy skeletal, mixed,
superactive, mesic, Typic Haploxerepts Pardaloe A§ 6.2 26.2 1.3 210 430 360 24.7
Entisol: fine silty, siliceous, active, acid,
thermic, Fluvaquentic Endoaquepts Pophers A¶ 4.3 50.7 4.3 10 600 390 3.6
Mollisol: clay-loamy, mesic, Argiudoll Unknown C 8.1 25.8 3.6 320 370 310 NA#
† Data from Trott, 1981.
‡ Includes only Ca and Mg.
§ Data from Munn and Singer, 1981, p. 93.
¶ Data from Midwood and Boutton, 1998.
# NA ⫽ not available.
capacity, and the microtiter plate was then placed in a vacuum expressed relative to atmospheric N2. The ␦13
C values are
expressed relative to Vienna-Pee Dee Belemnite (V-PDB).
desiccator (5 L). A beaker (150 mL) with 100 mL of concen-
trated (12 M) HCl was also placed inside the desiccator. The Controls (Ag capsules, HCl fumigation and water) were in-
cluded. The amounts of C and N in the controls never exceeded
samples were exposed to HCl vapor for times increasing in
6-h increments to 30 h, then 12-h increments to 96 h. After 2 g for N and 6 g for C, and were too low to obtain a reli-
able isotopic composition.
each exposure time, four replicates were removed from the
desiccator, dried at 60⬚C for 4 h, and the capsules were then One-way analysis of variance followed by the Student-New-
man Keuls test (SAS Institute, 1989) was used to test for differ-
closed.
The effects of HCl fumigation and HCl washing on total ences in total N, total C, ␦15
N, and ␦13
C between treatments.
soil N and C content and 13
C and 15
N abundance were deter-
mined on four carbonate-free soils: Auburn (loamy, mixed, su- Results and Discussion
peractive, thermic Lithic Haploxerepts), Chawanakee (loamy,
mixed, active, mesic, shallow Typic Dystroxerepts), Pardaloe A common practice for removing carbonates from
(loamy-skeletal, mixed, superactive, mesic Typic Haploxer- soil is washing the samples with dilute acid followed by
epts), and Pophers (fine-silty, siliceous, active, acid, thermic several washings with deionized water (Connin et al.,
Fluvaquentic Endoaquepts) (Table 1). Subsamples of the soil 1997; Midwood and Boutton, 1998; Collins et al., 1999).
were analyzed either untreated, exposed overnight to HCl
Although, the ␦13
C of the bulk soil organic C may be
vapor, or treated with 0.5 M HCl (10 mL g⫺1
soil) for 24 h
unaffected by this treatment (Midwood and Boutton,
followed by two washings with water.
1998), it is conceivable that the composition of isotopi-
The procedure of Midwood and Boutton (1998) was fol-
cally heterogeneous soils could be altered by this treat-
lowed for acid washing the soils. Briefly, 5 g of ball-milled
ment through removal of dissolved organic C. Fumigat-
soil was treated with 150 mL of 0.5 M HCl and the soil-acid
mixture stirred three times over a 24-h period. Soils were then ing the soil with HCl may reduce or eliminate any
washed twice with distilled water. Each time the water was organic C losses and minimize the potential for changes
replaced after a 24-h period. Soil was dried at 60⬚C, ground in ␦13
C.
by mortar and pestle, 30-mg samples were weighed into Ag Acid fumigation with HCl of the four noncalcareous
foil capsules, and the capsules closed for isotopic analysis. soils did not alter their 13
C compositions or their organic
Four replicates were used for all analyses.
C and total N contents (Table 2). Treating the same soils
Total N, total C, ␦15
N, and the ␦13
C for all samples were
with dilute HCl followed by washing led to significant
determined on a Europa 20-20 continuous flow isotope ratio
declines in total soil C and N for all four soils. For three
mass spectrometer (PDZ Europa Ltd., Sandbach, UK) follow-
soils, the ␦13
C value also became more negative. As the
ing combustion at 1000⬚C in a Europa ANCA-GSL CN ana-
lyzer (PDZ Europa Ltd., Sandbach, UK). The ␦15
N values are decline in the 13
C value for these three soils ranged
Table 2. Results of HCl fumigation and HCl washing on the elemental and isotopic N and C in soil (n ⫽ 4 replicates).
Soil name Treatment ␦15
N ␦13
C N C
‰ mg g⫺1
Auburn Untreated 1.80 ⫾ 0.01a† ⫺26.98 ⫾ 0.02a 2.528 ⫾ 0.000a 29.138 ⫾ 0.004a
HCl fumigation 1.84 ⫾ 0.05a ⫺26.93 ⫾ 0.01a 2.455 ⫾ 0.000a 28.960 ⫾ 0.008a
HCl washed 1.97 ⫾ 0.04b ⫺26.96 ⫾ 0.02a 2.366 ⫾ 0.001b 27.743 ⫾ 0.017b
Chawanakee Untreated 0.78 ⫾ 0.04a ⫺26.02 ⫾ 0.01a 1.286 ⫾ 0.002a 24.826 ⫾ 0.026a
HCl fumigation 0.84 ⫾ 0.03a ⫺26.04 ⫾ 0.01a 1.300 ⫾ 0.001a 25.124 ⫾ 0.007a
HCl washed 0.81 ⫾ 0.02a ⫺26.11 ⫾ 0.01b 1.174 ⫾ 0.002b 23.099 ⫾ 0.027b
Pardaloe Untreated 2.70 ⫾ 0.02a ⫺25.90 ⫾ 0.02a 1.251 ⫾ 0.000a 26.206 ⫾ 0.008a
HCl fumigation 2.81 ⫾ 0.03b ⫺25.92 ⫾ 0.01a 1.167 ⫾ 0.001a 26.147 ⫾ 0.018a
HCl washed 2.84 ⫾ 0.03b ⫺26.06 ⫾ 0.02b 1.139 ⫾ 0.001b 23.881 ⫾ 0.017b
Pophers Untreated 2.58 ⫾ 0.02a ⫺27.31 ⫾ 0.02a 4.260 ⫾ 0.002a 54.090 ⫾ 0.028a
HCl fumigation 2.64 ⫾ 0.01a ⫺27.28 ⫾ 0.01a 4.175 ⫾ 0.001a 53.158 ⫾ 0.026a
HCl washed 3.38 ⫾ 0.04b ⫺27.51 ⫾ 0.01b 3.570 ⫾ 0.004b 48.584 ⫾ 0.045b
† Means ⫾ S.E. within a column and followed by the same letter are not significantly different at P ⬍ 0.05.
3. NOTES 1855
HCl fumigation for 6 h. No further decline in the ␦13
C
value occurred for the remainder of the fumigation pe-
riod. At the same time, the total C content of the soil
declined from a maximum of 32 mg g⫺1
to 8 mg g⫺1
after
6 h of HCl fumigation and remained constant thereafter
(Fig. 1). However, Hedges and Stern (1984) found that
HCl-vapor treatment failed to remove all carbonate
from a soil with 50% carbonate, suggesting that HCl
fumigation may not always be completely effective in
removing carbonates from highly calcareous soils.
Water should be added to the dried soil prior to the
HCl fumigation. In dry soil, the rate at which carbonates
are converted into CO2 is low and the treatment may
fail to remove all carbonates from the soil (data not
Fig. 1. Changes in soil C concentration and the 13
C content of soil C shown). In this study, the addition of 50 L of distilled
following the removal of carbonates by HCl fumigation. The open
water to 30 mg of soil was sufficient to ensure complete
squares represent C values, the closed squares represent ␦13
C
removal of carbonates upon HCl fumigation.
values.
The HCl fumigation technique is a convenient and
easy method to remove inorganic C and could poten-
between 0.09 and 0.20‰, the removed C was enriched
tially also be used to determine the quantity of organic
in 13
C compared with residual organic C. When coralline
and inorganic C in a soil. One sample of the soil should
sediments that contained ⬎80% by weight CaCO3 were
be fumigated with HCl, another sample left untreated.
exposed to HCl fumigation, no contamination or loss
Following total C analysis, the difference in total C
of organic C and N occurred (Yamamuro and Kayanne,
content in the soil is attributed to carbonates. Total soil
1995). In contrast, when samples were acidified with 20
C content in the calcareous soil before HCl fumigation
mL of 1 M HCl and washed with distilled water, 20%
was 32 mg g⫺1
soil and decreased to 8 mg g⫺1
soil after
of the C and N was lost. Since those samples were not
HCl fumigation. Therefore, 24 mg g⫺1
soil or 75% of the
analyzed for isotopic composition, it remains unknown
total C content in the soil was in the form of inorganic C.
whether the 13
C and 15
N abundances were changed. Mid-
It should be pointed out that the HCl-fumigation
wood and Boutton (1998) used one of the carbonate-
method for determining inorganic C requires two sepa-
free soils included in this study (Pophers). When they
rate analyses: total organic and inorganic C (untreated
treated this soil with 1 M HCl for up to 8 d, followed
sample), and organic C (treated sample). As both analy-
by washing the soil with distilled water, organic C con-
ses contribute experimental error, the calculated amount
centration declined significantly but the ␦13
C value of
of inorganic C based on the difference between the C
the soil was unaffected.
in the two analyses may be less accurate than a direct
Acid fumigation always increased the ␦15
N value of
measurement. However, this point requires further re-
the soil but for only one soil (Pardaloe) was the increase
search.
significant at P ⬍ 0.05 (Table 2). For this soil, there was
Finally, it should be mentioned that the HCl-fumiga-
also a decrease, albeit not a significant one, in total N
tion method does reduce, by about 50%, the number
content following HCl fumigation (Table 2). Appar-
of samples that can be combusted before the elemental
ently, the amount of N lost remained small but its ␦15
N
analyzer combustion reactor is exhausted.
differed sufficiently to result in an increase in the ␦15
N
value of the bulk soil N following fumigation. Lohse et Conclusions
al. (2000) observed up to a 50% loss of N in continental
Fumigating soil with HCl vapor rather than washing
margin sediments following the elimination of carbon-
the soil in dilute HCl is an effective method to remove
ates by acidification using diluted H2SO4. Fumigation
carbonates prior to isotopic analysis. The method does
with HCl led to a small but consistent increases in the
not alter the ␦13
C signature of organic C and no losses
␦15
N value of soil N and losses of N can occur. Therefore,
of organic C occurred. In a soil that contained 75% of
some caution is recommended in the use of 15
N measure-
total C as carbonates, all carbonates were removed
ments from HCl-fumigated soils. Treating soil with di-
within a 6-h period of fumigation. A large number of
luted HCl followed by washing significantly reduced the
samples can be processed simultaneously. However,
N concentration for all soils tested, and the ␦15
N value
HCl fumigation may lead to a small increase in the ␦15
N
increased significantly in three of the four soils (Table
value of soil N.
2). Midwood and Boutton (1998) also observed signifi-
cant losses in N concentration when the Pophers soil Acknowledgments
was treated with HCl. Clearly, when soils are washed
We thank Dr. T. Boutton, Texas A&M University, and
with HCl, total N concentration can be reduced, and
Dr. M. Singer, University of California-Davis, for providing
the ␦15
N changed.
us with the carbonate-free soil samples. We also thank Chris
Exposure to concentrated HCl vapor for 6 h com- Hartley for the statistical analyses.
pletely removed carbonates from a soil that contained
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DIVISION S-10—WETLAND SOILS
Heterotrophic Microbial Activity in Northern Everglades Wetland Soils
A. L. Wright and K. R. Reddy*
ABSTRACT
Organic matter degradation plays an important
Phosphorus loading to the northern Florida Everglades has been role in nutrient cycling of wetlands. Rates of or-
implicated in causing changes in vegetation, peat accretion rates, and ganic matter degradation and CO2 production provide
other soil physical-chemical properties. Our study focused on de-
an indication of the microbial activity of soils, with pri-
termining the influence of P loading on aerobic and anaerobic hetero-
mary factors influencing microbial activity being con-
trophic microbial activities (measured as CO2 and CH4 production)
centrations of utilizable substrates, electron acceptors,
in detritus and soil collected from the Water Conservation Area 2a
and nutrients such as N and P (Webster and Benfield,
(WCA-2a) of the Everglades. Heterotrophic microbial activities mea-
sured under both field and laboratory conditions were higher in areas 1986; D’Angelo and Reddy, 1994; Reddy et al., 1999)
impacted by P loading as compared to the unimpacted interior marsh. A major limiting factor of microbial growth in short
Microbial heterotrophic activities were higher in detritus and surface term studies is the utilization of readily degradable com-
soils and decreased with depth. In field studies, CO2 production rates
pounds of the dissolved organic carbon (DOC) pool
in anaerobic soils were approximately 64% of those observed in aero-
(Hoppe, 1983). Short term studies primarily determine
bic soils. Additions of substrates containing C, N, and P generally
microbial respiration on the basis of the DOC pool. In
enhanced heterotrophic microbial activity. In laboratory studies in-
long term studies, utilizable portions of the DOC pool are
volving addition of various inorganic electron acceptors, increased
microbial activities in the order of O⫺
2 ⬎ NO⫺
3 ⫽ SO2⫺
4 ⬎ HCO⫺
3 were depleted, and heterotrophic microbial activity measure-
observed. Microbial CO2 production rates under denitrifying and sul- ments are often based on utilization of large organic com-
fate reducing conditions ranged from 30–42% and 29–44%, respec- pounds which must be acted upon by extracellular en-
tively, of aerobic rates. Methane production rates were only up to
zymes, resulting in lower microbial activity (Chrost, 1991).
9% of aerobic CO2 production rates. Both CO2 and CH4 production
Organic matter degradation in wetlands is often lim-
rates were significantly correlated with soil P parameters and micro-
ited by the availability of electron acceptors rather than
bial biomass. Enhanced heterotrophic microbial activities resulting
electron donors (Reddy and D’Angelo, 1994; Amador
from P loading has the potential to increase turnover of organic
and Jones, 1995; McLatchey and Reddy, 1998). Oxygen
matter which may lead to increased supply of bioavailable nutrients
to emergent macrophytes and periphyton and higher nutrient concen- is the most important electron acceptor in terrestrial
trations in the water column. systems, but is usually limited to the upper soil surface
and the overlying water column in wetlands. A sequen-
tial reduction of electron acceptors with depth in soils gen-
Wetland Biogeochmistry Laboratory, University of Florida, 106 New- erally proceeds in the order of O⫺
2 ⬎ NO⫺
3 ⬎ SO2⫺
4 ⬎
ell Hall, P.O. Box 110510, Gainesville, FL 32611. Received 22 Aug.
HCO⫺
3 on the basis of theoretical thermodynamic en-
2000. *Corresponding author (krr@ufl.edu).
ergy yields to microorganisms (Billen, 1982; Reddy and
D’Angelo, 1994). Thus, degradation rates of DOC in
Published in Soil Sci. Soc. Am. J. 65:1856–1864 (2001).