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Plant Nutrition
Plant Nutrition 
 In this topic you will learn: 
 Photosynthesis is the fundamental process by 
which plants manufacture simple sugars from raw 
materials.
Starter Question 
 A tree is planted in a 
meadow. After 20 
years it has grown into a 
big tree, weighing 250kg 
more than when it was 
planted. 
 Where do the extra 
250kg come from? 
 Explain your answer as 
fully as you can.
Learning Outcomes 
 Define photosynthesis and state the equation 
for the production of simple sugars and 
oxygen in words. 
 State the equation for photosynthesis in 
symbols
Plant Nutrition 
 Photosynthesis is the process in which light 
energy, trapped by chlorophyll, is used to 
convert carbon dioxide and water into glucose 
and oxygen. 
 Word Equation 
Light energy 
Carbon dioxide + water glucose + oxygen 
Chlorophyll
Photosynthesis 
Word equation 
Light energy 
Carbon dioxide + water glucose + oxygen 
chlorophyll 
Balanced Symbol Equation 
Light energy 
6CO2 + 6H2O C6H12O6 + 6O2 
chlorophyll
Learning Outcome 
 Describe the intake of raw materials, the 
trapping and storing of energy (conversion of 
light energy into chemical energy), the 
formation of food substances and their 
subsequent storage.
THINK!! 
 What are the raw materials in 
photosynthesis? 
 Where does the plant get them from? 
 What are the products of photosynthesis? 
 What happens to them?
Process of photosynthesis 
 Carbon dioxide diffuses into the leaves of 
green plants 
 Water is absorbed by osmosis through plant’s 
roots and transported to the leaf through 
xylem vessels 
 Chloroplasts, containing chlorophyll, are 
responsible for trapping light energy (energy 
is used to break up water molecules and then 
bond hydrogen and carbon dioxide to form 
glucose)
Process of photosynthesis 
 Glucose is usually changed to sucrose for 
transport around the plant, or to starch for 
storage 
 Oxygen is released as a waste product.
Leaves – a factory for 
photosynthesis 
 Leaves are adapted to make them very 
efficient for photosynthesis 
Network of veins 
carry water and 
minerals to the 
leaf and food 
away from it. 
Leaf is green, as it contains 
chlorophyll to absorb light 
Leaf is broad to give a 
large surface area to 
absorb light 
Petiole holds the 
leaf at an angle 
so it faces the sun 
and absorbs as 
much light as 
possible 
Leaf is thin to allow 
substances to move 
quickly between 
and into cells 
(diffusion)
Testing a leaf for starch 
 Dip a leaf into boiling water for 
about a minute to soften it. 
 Turn off the Bunsen burner. 
 Put the leaf into a test-tube of 
ethanol. 
 Stand the test-tube in a beaker of 
hot water for about 10 minutes. 
 Wash the leaf in cold water 
 Spread the leaf out flat on a petri 
dish and cover it with iodine 
solution. 
 If the leaf goes blue-black, starch 
is present.
Is light needed for photosynthesis? 
 Take a de-starched geranium plant. 
 Cover part of a leaf with some tin foil (this 
prevents light getting through). 
 Leave the plant in sunlight for a few hours 
 Test the leaf for starch.
Is light needed for photosynthesis? 
Questions: 
 Which parts of the leaf went blue black? 
 Why do parts that were not covered contain 
starch?
Photosynthesis
Is carbon dioxide needed for 
photosynthesis? 
 Take a de starched 
geranium plant 
 Enclose it in a plastic bag 
with a chemical that 
absorbs carbon dioxide. (e.g. 
soda lime or sodium 
hydroxide pellets). 
 Leave the plant in sunlight 
for a couple of hours 
 Test the leaf for starch.
Is carbon dioxide needed for 
photosynthesis? 
Questions: 
 Does the leaf contain starch? Why not? 
 Has the plant carried out photosynthesis? 
 What would be your control plant? 
 (Hint: a control plant should have everything it needs for 
photosynthesis including carbon dioxide).
Learning Outcomes 
 Define the term limiting factor and interpret 
(as limiting factors that affect 
photosynthesis) the effects of light and 
carbon dioxide concentration.
Limiting Factors 
 The factors which if increased will increase 
the rate of photosynthesis. 
 The process of photosynthesis depends on: 
 Availability of light 
 Presence of a pigment to absorb the light 
 Supply of carbon dioxide and water 
 A temperature suitable for enzyme activity
Limiting factors 
 If any of these factors are in short supply 
the rate of photosynthesis will be less then 
its maximum possible rate 
 The factor furthest from it’s optimum level is 
controlling the overall rate of photosynthesis!
The effect of light on the rate of 
photosynthesis 
 Graph 
 As light intensity increase, so does the rate 
of photosynthesis, until a point where another 
factor becomes limiting 
 If the limiting factor in the graph was carbon 
dioxide concentration and the plants were 
given more carbon dioxide the graph would 
level off at a higher rate of photosynthesis.
The effects of carbon dioxide on 
the rate of photosynthesis 
 graph
Effect of temperature on the 
rate of photosynthesis 
 Temperature affects the enzymes that 
control the rates of the chemical reactions of 
photosynthesis. 
 Graph 
 Compare this graph to the graphs showing the 
effect of temperature on enzyme activity.
Learning Outcome 
 Explain the use of carbon dioxide enrichment, 
optimum light and optimum temperatures in 
greenhouse systems
Optimum conditions in 
greenhouse systems 
 Greenhouses are used to control the 
conditions for plant growth 
 The atmospheric conditions can be controlled 
and the glass traps heat inside
Carbon dioxide enrichment 
 0.04% carbon dioxide in the air, so it can 
easily limit the rate of photosynthesis 
 Carbon dioxide concentration in a greenhouse 
can be increased by: 
 Burning fossil fuels 
 Releasing pure carbon dioxide from a gas cylinder
Optimum light and temperature 
 Optimum light 
 Light conditions for growth can be improved by 
using artificial lights 
 Optimum temperature 
 Can be raised by using a heating system
Learning outcomes 
 Identify the cellular and tissue structure if a 
dicotyledonous leaf, as seen in cross section
Leaf Structure 
 Revision: 
 How external features if a leaf design it to be a 
factory for photosynthesis 
 Activity – Bioviewers 
 Observe and draw a cross section of a 
dicotyledonous leaf
Internal Structure of a leaf 
 Use the words below to 
label your diagram of a 
leaf 
 Waxy cuticle 
 Upper epidermis 
 Palisade mesophyll 
 Spongy mesophyll 
 Lower epidermis 
 Guard cell 
 Stoma 
 Phloem 
 xylem
Functions of structures 
 Pupil Activity 
 Cut out the internal structures and functions of a 
leaf 
 Match up the structure to the function
Functions 
Structure Function 
Waxy cuticle Prevents water loss from the leaf 
Upper epidermis Transparent, Allows light to travel to cells within 
the leaf 
Palisade mesophyll Cells tightly packed together, Main region of 
photosynthesis, Cells packed with chloroplasts 
Spongy mesophyll Cells spherical and loosely packed, air spaces 
between cells allow for gas exchange 
Vascular bundle Contains the xylem (transports water and minerals 
to the leaf) and phloem (translocation) 
Lower epidermis Acts as a protective layer 
stomata Regulates water loss (transpiration) 
Site of gaseous exchange in the leaf 
Guard cells Control the opening and closing of stomata
Annotating a diagram 
 Using the information you have already been 
provided with 
 Label the diagram 
 Write a statement for each structure that you 
have labelled.
Learning Outcomes 
 Describe the importance of nitrate ions for 
protein synthesis and magnesium ions for 
chlorophyll synthesis 
 Explain the effects of nitrate ions and 
magnesium ion deficiency on plant growth 
 Describe the uses and the dangers of overuse 
of nitrogen fertilisers
Nitrate ions 
 Importance 
 Needed for 
synthesising amino 
acids 
 Amino acids form 
long chains to make 
proteins 
 Deficiency 
 Plant growth is 
stunted 
 Weak stem 
 Yellow, dying lower 
leaves 
 Upper leaves turn 
pale green
Magnesium ions 
 Importance 
 Forms part of 
chlorophyll molecules 
 Plants need 
chlorophyll to trap 
light to provide 
energy for 
photosynthesis 
 Deficiency 
 Chlorosis 
 Leaves turn yellow 
 Lack of 
photosynthesis limits 
plant growth
Nitrogen fertilisers 
 Nitrogen fertilisers can be used 
 To increase crop yields 
 To replace the nitrate ions removed by intensive 
farming 
 Nitrate can also be replaced by 
 Applying animal manure 
 Crop rotation 
 Grow leguminous plants in the field every 2/3 years
Dangers of overuse of fertilisers 
 Too much nitrogen fertiliser can cause plant 
roots to lose water by osmosis 
 The plant wilts and dies 
 Eutrophication 
 Fertilisers are soluble in rainwater and are washed 
out of the soil 
 This is known as leaching
Eutrophication
Stages of Eutrophication 
 Fertilisers used by farmers may be washed into lakes 
and rivers 
 Rapid growth of water plants cause by the fertilisers 
 Death of some of these plants due to lack of light 
from overcrowding 
 Microbes which feed on the dead organisms now 
increase in number 
 Oxygen is used up quickly by the microbes 
 Suffocation of fish and other aquatic animals due to 
the lack of oxygen in the water.
Pupil Activity 
 Using the 5 main stages of eutrophication 
 leaching 
 Rapid algal growth 
 Death of algae 
 Decay by bacteria 
 Death of aquatic animals 
 Design a cartoon strip that summarises the 
process of Eutrophication
Progress Question – easy ones 
1. Plants need a supply of nitrate ions. State 
the use made of nitrate ions in plants. [1] 
2. Many farmers regularly add nitrate 
fertilisers to their fields. Explain why this 
is necessary. [2]
Progress Question – difficult one 
3. A farmer spreads a nitrate-rich fertiliser 
over his fields. Each time he does this, he 
washes out his spreading equipment in a farm 
pond. 
Suggest and explain what the likely effects 
of such pollution will be on the plants and 
animals in the pond. [5]

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Plant nutrition

  • 2. Plant Nutrition  In this topic you will learn:  Photosynthesis is the fundamental process by which plants manufacture simple sugars from raw materials.
  • 3. Starter Question  A tree is planted in a meadow. After 20 years it has grown into a big tree, weighing 250kg more than when it was planted.  Where do the extra 250kg come from?  Explain your answer as fully as you can.
  • 4. Learning Outcomes  Define photosynthesis and state the equation for the production of simple sugars and oxygen in words.  State the equation for photosynthesis in symbols
  • 5. Plant Nutrition  Photosynthesis is the process in which light energy, trapped by chlorophyll, is used to convert carbon dioxide and water into glucose and oxygen.  Word Equation Light energy Carbon dioxide + water glucose + oxygen Chlorophyll
  • 6. Photosynthesis Word equation Light energy Carbon dioxide + water glucose + oxygen chlorophyll Balanced Symbol Equation Light energy 6CO2 + 6H2O C6H12O6 + 6O2 chlorophyll
  • 7. Learning Outcome  Describe the intake of raw materials, the trapping and storing of energy (conversion of light energy into chemical energy), the formation of food substances and their subsequent storage.
  • 8. THINK!!  What are the raw materials in photosynthesis?  Where does the plant get them from?  What are the products of photosynthesis?  What happens to them?
  • 9. Process of photosynthesis  Carbon dioxide diffuses into the leaves of green plants  Water is absorbed by osmosis through plant’s roots and transported to the leaf through xylem vessels  Chloroplasts, containing chlorophyll, are responsible for trapping light energy (energy is used to break up water molecules and then bond hydrogen and carbon dioxide to form glucose)
  • 10. Process of photosynthesis  Glucose is usually changed to sucrose for transport around the plant, or to starch for storage  Oxygen is released as a waste product.
  • 11. Leaves – a factory for photosynthesis  Leaves are adapted to make them very efficient for photosynthesis Network of veins carry water and minerals to the leaf and food away from it. Leaf is green, as it contains chlorophyll to absorb light Leaf is broad to give a large surface area to absorb light Petiole holds the leaf at an angle so it faces the sun and absorbs as much light as possible Leaf is thin to allow substances to move quickly between and into cells (diffusion)
  • 12. Testing a leaf for starch  Dip a leaf into boiling water for about a minute to soften it.  Turn off the Bunsen burner.  Put the leaf into a test-tube of ethanol.  Stand the test-tube in a beaker of hot water for about 10 minutes.  Wash the leaf in cold water  Spread the leaf out flat on a petri dish and cover it with iodine solution.  If the leaf goes blue-black, starch is present.
  • 13. Is light needed for photosynthesis?  Take a de-starched geranium plant.  Cover part of a leaf with some tin foil (this prevents light getting through).  Leave the plant in sunlight for a few hours  Test the leaf for starch.
  • 14. Is light needed for photosynthesis? Questions:  Which parts of the leaf went blue black?  Why do parts that were not covered contain starch?
  • 16. Is carbon dioxide needed for photosynthesis?  Take a de starched geranium plant  Enclose it in a plastic bag with a chemical that absorbs carbon dioxide. (e.g. soda lime or sodium hydroxide pellets).  Leave the plant in sunlight for a couple of hours  Test the leaf for starch.
  • 17. Is carbon dioxide needed for photosynthesis? Questions:  Does the leaf contain starch? Why not?  Has the plant carried out photosynthesis?  What would be your control plant?  (Hint: a control plant should have everything it needs for photosynthesis including carbon dioxide).
  • 18. Learning Outcomes  Define the term limiting factor and interpret (as limiting factors that affect photosynthesis) the effects of light and carbon dioxide concentration.
  • 19. Limiting Factors  The factors which if increased will increase the rate of photosynthesis.  The process of photosynthesis depends on:  Availability of light  Presence of a pigment to absorb the light  Supply of carbon dioxide and water  A temperature suitable for enzyme activity
  • 20. Limiting factors  If any of these factors are in short supply the rate of photosynthesis will be less then its maximum possible rate  The factor furthest from it’s optimum level is controlling the overall rate of photosynthesis!
  • 21. The effect of light on the rate of photosynthesis  Graph  As light intensity increase, so does the rate of photosynthesis, until a point where another factor becomes limiting  If the limiting factor in the graph was carbon dioxide concentration and the plants were given more carbon dioxide the graph would level off at a higher rate of photosynthesis.
  • 22. The effects of carbon dioxide on the rate of photosynthesis  graph
  • 23. Effect of temperature on the rate of photosynthesis  Temperature affects the enzymes that control the rates of the chemical reactions of photosynthesis.  Graph  Compare this graph to the graphs showing the effect of temperature on enzyme activity.
  • 24. Learning Outcome  Explain the use of carbon dioxide enrichment, optimum light and optimum temperatures in greenhouse systems
  • 25. Optimum conditions in greenhouse systems  Greenhouses are used to control the conditions for plant growth  The atmospheric conditions can be controlled and the glass traps heat inside
  • 26. Carbon dioxide enrichment  0.04% carbon dioxide in the air, so it can easily limit the rate of photosynthesis  Carbon dioxide concentration in a greenhouse can be increased by:  Burning fossil fuels  Releasing pure carbon dioxide from a gas cylinder
  • 27. Optimum light and temperature  Optimum light  Light conditions for growth can be improved by using artificial lights  Optimum temperature  Can be raised by using a heating system
  • 28. Learning outcomes  Identify the cellular and tissue structure if a dicotyledonous leaf, as seen in cross section
  • 29. Leaf Structure  Revision:  How external features if a leaf design it to be a factory for photosynthesis  Activity – Bioviewers  Observe and draw a cross section of a dicotyledonous leaf
  • 30. Internal Structure of a leaf  Use the words below to label your diagram of a leaf  Waxy cuticle  Upper epidermis  Palisade mesophyll  Spongy mesophyll  Lower epidermis  Guard cell  Stoma  Phloem  xylem
  • 31. Functions of structures  Pupil Activity  Cut out the internal structures and functions of a leaf  Match up the structure to the function
  • 32. Functions Structure Function Waxy cuticle Prevents water loss from the leaf Upper epidermis Transparent, Allows light to travel to cells within the leaf Palisade mesophyll Cells tightly packed together, Main region of photosynthesis, Cells packed with chloroplasts Spongy mesophyll Cells spherical and loosely packed, air spaces between cells allow for gas exchange Vascular bundle Contains the xylem (transports water and minerals to the leaf) and phloem (translocation) Lower epidermis Acts as a protective layer stomata Regulates water loss (transpiration) Site of gaseous exchange in the leaf Guard cells Control the opening and closing of stomata
  • 33. Annotating a diagram  Using the information you have already been provided with  Label the diagram  Write a statement for each structure that you have labelled.
  • 34. Learning Outcomes  Describe the importance of nitrate ions for protein synthesis and magnesium ions for chlorophyll synthesis  Explain the effects of nitrate ions and magnesium ion deficiency on plant growth  Describe the uses and the dangers of overuse of nitrogen fertilisers
  • 35. Nitrate ions  Importance  Needed for synthesising amino acids  Amino acids form long chains to make proteins  Deficiency  Plant growth is stunted  Weak stem  Yellow, dying lower leaves  Upper leaves turn pale green
  • 36. Magnesium ions  Importance  Forms part of chlorophyll molecules  Plants need chlorophyll to trap light to provide energy for photosynthesis  Deficiency  Chlorosis  Leaves turn yellow  Lack of photosynthesis limits plant growth
  • 37. Nitrogen fertilisers  Nitrogen fertilisers can be used  To increase crop yields  To replace the nitrate ions removed by intensive farming  Nitrate can also be replaced by  Applying animal manure  Crop rotation  Grow leguminous plants in the field every 2/3 years
  • 38. Dangers of overuse of fertilisers  Too much nitrogen fertiliser can cause plant roots to lose water by osmosis  The plant wilts and dies  Eutrophication  Fertilisers are soluble in rainwater and are washed out of the soil  This is known as leaching
  • 40. Stages of Eutrophication  Fertilisers used by farmers may be washed into lakes and rivers  Rapid growth of water plants cause by the fertilisers  Death of some of these plants due to lack of light from overcrowding  Microbes which feed on the dead organisms now increase in number  Oxygen is used up quickly by the microbes  Suffocation of fish and other aquatic animals due to the lack of oxygen in the water.
  • 41. Pupil Activity  Using the 5 main stages of eutrophication  leaching  Rapid algal growth  Death of algae  Decay by bacteria  Death of aquatic animals  Design a cartoon strip that summarises the process of Eutrophication
  • 42. Progress Question – easy ones 1. Plants need a supply of nitrate ions. State the use made of nitrate ions in plants. [1] 2. Many farmers regularly add nitrate fertilisers to their fields. Explain why this is necessary. [2]
  • 43. Progress Question – difficult one 3. A farmer spreads a nitrate-rich fertiliser over his fields. Each time he does this, he washes out his spreading equipment in a farm pond. Suggest and explain what the likely effects of such pollution will be on the plants and animals in the pond. [5]