Explain the mechanisms of internal loading of phosphorus in lakes.pdfrastogiarun
Explain the meaning of the following dimensionless numbers respectively.
Nu, Re, Pr, Gr
Solution
Nusselt number (Nu) is a non dimensional heat transfer coefficient. It is a measure of heat
transfer rate comparison between the conduction and convection.
Nu = h*L / kf
It is the ratio of convection to pure conduction heat transfer, where kf is the conductivity of the
fluid.
Reynolds number (Re) says, whether the flow is inertial or viscous force dominant that leads to
draw characteristics of fluid whether the flow is laminar or turbulent.
Re = v*L / kinematic viscosity
It is the ratio of the inertia and viscous forces.
Viscous force provides the dampening effect for disturbances in the fluid.
If dampening is laminar flow --> strong enough
Otherwise, instability è turbulent flow --> critical Reynolds number
Prandtl (Pr) number says about the kind of fluid. It can give the information about the thickness
of thermal and hydrodynamic boundary layer.
Pr = kinematic viscosity/thermal diffusivity
It is the ratio of the momentum and thermal diffusivities.
Grashof number is the ratio of buoyancy force to viscous force and plays nearly the same role as
the Reynolds number in forced convection.
Gr = buoyancy force / viscous force
For forced convection, the heat transfer correlation can be expressed as Nu=f (Re, Pr).
Nutrient cycling is one of the most important processes that occur in an ecosystem. The nutrient cycle describes the use, movement, and recycling of nutrients in the environment.
Richard's aventures in two entangled wonderlandsRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
Nutraceutical market, scope and growth: Herbal drug technologyLokesh Patil
As consumer awareness of health and wellness rises, the nutraceutical market—which includes goods like functional meals, drinks, and dietary supplements that provide health advantages beyond basic nutrition—is growing significantly. As healthcare expenses rise, the population ages, and people want natural and preventative health solutions more and more, this industry is increasing quickly. Further driving market expansion are product formulation innovations and the use of cutting-edge technology for customized nutrition. With its worldwide reach, the nutraceutical industry is expected to keep growing and provide significant chances for research and investment in a number of categories, including vitamins, minerals, probiotics, and herbal supplements.
Introduction:
RNA interference (RNAi) or Post-Transcriptional Gene Silencing (PTGS) is an important biological process for modulating eukaryotic gene expression.
It is highly conserved process of posttranscriptional gene silencing by which double stranded RNA (dsRNA) causes sequence-specific degradation of mRNA sequences.
dsRNA-induced gene silencing (RNAi) is reported in a wide range of eukaryotes ranging from worms, insects, mammals and plants.
This process mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, and regulates the expression of protein-coding genes.
What are small ncRNAs?
micro RNA (miRNA)
short interfering RNA (siRNA)
Properties of small non-coding RNA:
Involved in silencing mRNA transcripts.
Called “small” because they are usually only about 21-24 nucleotides long.
Synthesized by first cutting up longer precursor sequences (like the 61nt one that Lee discovered).
Silence an mRNA by base pairing with some sequence on the mRNA.
Discovery of siRNA?
The first small RNA:
In 1993 Rosalind Lee (Victor Ambros lab) was studying a non- coding gene in C. elegans, lin-4, that was involved in silencing of another gene, lin-14, at the appropriate time in the
development of the worm C. elegans.
Two small transcripts of lin-4 (22nt and 61nt) were found to be complementary to a sequence in the 3' UTR of lin-14.
Because lin-4 encoded no protein, she deduced that it must be these transcripts that are causing the silencing by RNA-RNA interactions.
Types of RNAi ( non coding RNA)
MiRNA
Length (23-25 nt)
Trans acting
Binds with target MRNA in mismatch
Translation inhibition
Si RNA
Length 21 nt.
Cis acting
Bind with target Mrna in perfect complementary sequence
Piwi-RNA
Length ; 25 to 36 nt.
Expressed in Germ Cells
Regulates trnasposomes activity
MECHANISM OF RNAI:
First the double-stranded RNA teams up with a protein complex named Dicer, which cuts the long RNA into short pieces.
Then another protein complex called RISC (RNA-induced silencing complex) discards one of the two RNA strands.
The RISC-docked, single-stranded RNA then pairs with the homologous mRNA and destroys it.
THE RISC COMPLEX:
RISC is large(>500kD) RNA multi- protein Binding complex which triggers MRNA degradation in response to MRNA
Unwinding of double stranded Si RNA by ATP independent Helicase
Active component of RISC is Ago proteins( ENDONUCLEASE) which cleave target MRNA.
DICER: endonuclease (RNase Family III)
Argonaute: Central Component of the RNA-Induced Silencing Complex (RISC)
One strand of the dsRNA produced by Dicer is retained in the RISC complex in association with Argonaute
ARGONAUTE PROTEIN :
1.PAZ(PIWI/Argonaute/ Zwille)- Recognition of target MRNA
2.PIWI (p-element induced wimpy Testis)- breaks Phosphodiester bond of mRNA.)RNAse H activity.
MiRNA:
The Double-stranded RNAs are naturally produced in eukaryotic cells during development, and they have a key role in regulating gene expression .
This presentation explores a brief idea about the structural and functional attributes of nucleotides, the structure and function of genetic materials along with the impact of UV rays and pH upon them.
Cancer cell metabolism: special Reference to Lactate PathwayAADYARAJPANDEY1
Normal Cell Metabolism:
Cellular respiration describes the series of steps that cells use to break down sugar and other chemicals to get the energy we need to function.
Energy is stored in the bonds of glucose and when glucose is broken down, much of that energy is released.
Cell utilize energy in the form of ATP.
The first step of respiration is called glycolysis. In a series of steps, glycolysis breaks glucose into two smaller molecules - a chemical called pyruvate. A small amount of ATP is formed during this process.
Most healthy cells continue the breakdown in a second process, called the Kreb's cycle. The Kreb's cycle allows cells to “burn” the pyruvates made in glycolysis to get more ATP.
The last step in the breakdown of glucose is called oxidative phosphorylation (Ox-Phos).
It takes place in specialized cell structures called mitochondria. This process produces a large amount of ATP. Importantly, cells need oxygen to complete oxidative phosphorylation.
If a cell completes only glycolysis, only 2 molecules of ATP are made per glucose. However, if the cell completes the entire respiration process (glycolysis - Kreb's - oxidative phosphorylation), about 36 molecules of ATP are created, giving it much more energy to use.
IN CANCER CELL:
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
introduction to WARBERG PHENOMENA:
WARBURG EFFECT Usually, cancer cells are highly glycolytic (glucose addiction) and take up more glucose than do normal cells from outside.
Otto Heinrich Warburg (; 8 October 1883 – 1 August 1970) In 1931 was awarded the Nobel Prize in Physiology for his "discovery of the nature and mode of action of the respiratory enzyme.
WARNBURG EFFECT : cancer cells under aerobic (well-oxygenated) conditions to metabolize glucose to lactate (aerobic glycolysis) is known as the Warburg effect. Warburg made the observation that tumor slices consume glucose and secrete lactate at a higher rate than normal tissues.
This pdf is about the Schizophrenia.
For more details visit on YouTube; @SELF-EXPLANATORY;
https://www.youtube.com/channel/UCAiarMZDNhe1A3Rnpr_WkzA/videos
Thanks...!
Comparing Evolved Extractive Text Summary Scores of Bidirectional Encoder Rep...University of Maribor
Slides from:
11th International Conference on Electrical, Electronics and Computer Engineering (IcETRAN), Niš, 3-6 June 2024
Track: Artificial Intelligence
https://www.etran.rs/2024/en/home-english/
Slide 1: Title Slide
Extrachromosomal Inheritance
Slide 2: Introduction to Extrachromosomal Inheritance
Definition: Extrachromosomal inheritance refers to the transmission of genetic material that is not found within the nucleus.
Key Components: Involves genes located in mitochondria, chloroplasts, and plasmids.
Slide 3: Mitochondrial Inheritance
Mitochondria: Organelles responsible for energy production.
Mitochondrial DNA (mtDNA): Circular DNA molecule found in mitochondria.
Inheritance Pattern: Maternally inherited, meaning it is passed from mothers to all their offspring.
Diseases: Examples include Leber’s hereditary optic neuropathy (LHON) and mitochondrial myopathy.
Slide 4: Chloroplast Inheritance
Chloroplasts: Organelles responsible for photosynthesis in plants.
Chloroplast DNA (cpDNA): Circular DNA molecule found in chloroplasts.
Inheritance Pattern: Often maternally inherited in most plants, but can vary in some species.
Examples: Variegation in plants, where leaf color patterns are determined by chloroplast DNA.
Slide 5: Plasmid Inheritance
Plasmids: Small, circular DNA molecules found in bacteria and some eukaryotes.
Features: Can carry antibiotic resistance genes and can be transferred between cells through processes like conjugation.
Significance: Important in biotechnology for gene cloning and genetic engineering.
Slide 6: Mechanisms of Extrachromosomal Inheritance
Non-Mendelian Patterns: Do not follow Mendel’s laws of inheritance.
Cytoplasmic Segregation: During cell division, organelles like mitochondria and chloroplasts are randomly distributed to daughter cells.
Heteroplasmy: Presence of more than one type of organellar genome within a cell, leading to variation in expression.
Slide 7: Examples of Extrachromosomal Inheritance
Four O’clock Plant (Mirabilis jalapa): Shows variegated leaves due to different cpDNA in leaf cells.
Petite Mutants in Yeast: Result from mutations in mitochondrial DNA affecting respiration.
Slide 8: Importance of Extrachromosomal Inheritance
Evolution: Provides insight into the evolution of eukaryotic cells.
Medicine: Understanding mitochondrial inheritance helps in diagnosing and treating mitochondrial diseases.
Agriculture: Chloroplast inheritance can be used in plant breeding and genetic modification.
Slide 9: Recent Research and Advances
Gene Editing: Techniques like CRISPR-Cas9 are being used to edit mitochondrial and chloroplast DNA.
Therapies: Development of mitochondrial replacement therapy (MRT) for preventing mitochondrial diseases.
Slide 10: Conclusion
Summary: Extrachromosomal inheritance involves the transmission of genetic material outside the nucleus and plays a crucial role in genetics, medicine, and biotechnology.
Future Directions: Continued research and technological advancements hold promise for new treatments and applications.
Slide 11: Questions and Discussion
Invite Audience: Open the floor for any questions or further discussion on the topic.
(May 29th, 2024) Advancements in Intravital Microscopy- Insights for Preclini...Scintica Instrumentation
Intravital microscopy (IVM) is a powerful tool utilized to study cellular behavior over time and space in vivo. Much of our understanding of cell biology has been accomplished using various in vitro and ex vivo methods; however, these studies do not necessarily reflect the natural dynamics of biological processes. Unlike traditional cell culture or fixed tissue imaging, IVM allows for the ultra-fast high-resolution imaging of cellular processes over time and space and were studied in its natural environment. Real-time visualization of biological processes in the context of an intact organism helps maintain physiological relevance and provide insights into the progression of disease, response to treatments or developmental processes.
In this webinar we give an overview of advanced applications of the IVM system in preclinical research. IVIM technology is a provider of all-in-one intravital microscopy systems and solutions optimized for in vivo imaging of live animal models at sub-micron resolution. The system’s unique features and user-friendly software enables researchers to probe fast dynamic biological processes such as immune cell tracking, cell-cell interaction as well as vascularization and tumor metastasis with exceptional detail. This webinar will also give an overview of IVM being utilized in drug development, offering a view into the intricate interaction between drugs/nanoparticles and tissues in vivo and allows for the evaluation of therapeutic intervention in a variety of tissues and organs. This interdisciplinary collaboration continues to drive the advancements of novel therapeutic strategies.
What is greenhouse gasses and how many gasses are there to affect the Earth.moosaasad1975
What are greenhouse gasses how they affect the earth and its environment what is the future of the environment and earth how the weather and the climate effects.
2. Biogeochemical Cycle
Importance of Phosphorous
Global cycling of Phosphorous
Human Impacts on Phosphorous cycle
Eutrophication
3. Energy flow through an ecosystem and is dissipated as heat, but chemical
elements are recycled.
The ways in which an element or compound such as water moves between its
various living and non-living forms and locations in the biosphere is called a
biogeochemical cycle
Biogeochemical cycles important to living organisms includes –
Water, Carbon, Nitrogen, Sulphur and Phosphorus
6. Phosphorous contributes about 0.1% weight percent and Cosmic
abundance of about 1 atom per 100 silicon atom;
Phosphorus is an essential nutrient for living organisms
It’s a key part of nucleic acid, like DNA and of phospholipids that
form our cell membranes.
As calcium phosphate , it also makes up the supportive components
of our bones
7. In nature , Phosphorus is often the Limiting nutrient- in other words, the
nutrient that’s in shortest supply and puts a limit on growth –and this is
particularly true for aquatic and fresh water ecosystem.
8. The phosphorus cycle is slow compared to other biogeochemical cycles such as the
water, carbon, and nitrogen cycles.
In nature, phosphorus is found mostly in the form of phosphate ions PO43-
9.
10. Phosphate compounds are found in sedimentary rocks, and as the rocks
weather—wear down over long time periods—the phosphorus they contain slowly
leaches into surface water and soils. Volcanic ash, aerosols, and mineral dust can
also be significant phosphate sources, though phosphorus has no real gas phase,
unlike other elements such as carbon, nitrogen, and sulfur.
Phosphate compounds in the soil can be taken up by plants and, from there,
transferred to animals that eat the plants. When plants and animals excrete
wastes or die, phosphates may be taken up by detritivores or returned to the soil.
Phosphorus-containing compounds may also be carried in surface runoff to rivers,
lakes, and oceans, where they are taken up by aquatic organisms.
When phosphorus-containing compounds from the bodies or wastes of marine
organisms sink to the floor of the ocean, they form new sedimentary layers. Over
long periods of time, phosphorus-containing sedimentary rock may be moved
from the ocean to the land by a geological process called uplift. However, this
process is very slow, and the average phosphate ion has an oceanic residence
time—time in the ocean—of 20,000 to 100,000 years.
11. Initially, phosphate weathers from rocks. The small losses in a terrestrial system
caused by leaching through the action of rain are balanced in the gains from
weathering rocks.
In soil, phosphate is absorbed on clay surfaces and organic matter particles and
becomes incorporated (immobilized). Plants dissolve ionized forms of phosphate.
The dead remains of plants get decayed and supplies Phosphate back to the soil
12. The ecosystem phase of the phosphorus cycle moves faster than the sediment
phase.
All organisms require phosphorus for synthesizing phospholipids, NADPH, ATP,
nucleic acids, and other compounds. Plants absorb phosphorus very quickly, and
then herbivores get phosphorus by eat plants. Then carnivores get phosphorus by
eating herbivores.
Eventually both of these organisms will excrete phosphorus as a waste. This
decomposition will release phosphorus into the soil. Plants absorb the phosphorus
from the soil and they recycle it within the ecosystem.
13. Unlike the other cycles, there is no volatile phosphorus-containing product to
return phosphorus to the atmosphere in the way carbon dioxide, nitrogen gas, and
sulfur dioxide are returned.
Therefore, phosphorus tends to accumulate in the seas. It can be retrieved by
mining the above-ground sediments of ancient seas, mostly as deposits of calcium
phosphate.
Seabirds also mine phosphorus from the sea by eating phosphorus-containing fish
and depositing it as guano (bird droppings).
Certain small islands inhabited by such birds have long been mined for these
deposits as a source of phosphorus for fertilizers.
14. The availability of phosphorus in an ecosystem is restricted by the rate of release
of this element during weathering.
The release of phosphorus from apatite dissolution is a key control on ecosystem
productivity.
The primary mineral with significant phosphorus content, apatite [Ca5(PO4)3OH]
undergoes carbonation.
Little of this released phosphorus is taken by biota (organic form) whereas, large
proportion reacts with other soil minerals leading to precipitation in unavailable
forms.
15.
16. Humans have greatly influenced the Phosphorus cycle by mining Phosphorous,
converting it to fertilizer, and by shipping fertilizer and products around the globe.
Transporting Phosphorus rich food from farms to cities has made a major change
in the global Phosphorous cycle.
Waters are enriched in Phosphorous from farms run off, and from effluents that
are inadequately treated before get discharged into water bodies.
Like nitrogen, increased use of fertilizers increases phosphorus runoff into our
waterways .Which contributes to eutrophication
17. Natural eutrophication is a process by which lakes gradually age and become
more productive and may take thousands of years to progress.
Cultural or anthropogenic eutrophication, however, is water pollution caused by
excessive plant nutrients, which results in excessive growth in algae population..
18. Some algae make water taste or smell bad or produce toxic compounds. Also, when all of
those algae die and are decomposed by microbes, large amounts of oxygen are used up as
their bodies are broken down. This spike in oxygen usage can sharply lower dissolved
oxygen levels in the water and may lead to death by hypoxia—lack of oxygen—for other
aquatic organisms, such as shellfish and finfish.
Regions of lakes and oceans that are depleted of oxygen due to a nutrient influx are called
dead zones. The number of dead zones has increased for several years, and more than 400
of these zones existed in 2008. One of the worst dead zones is off the coast of the United
States in the Gulf of Mexico. Fertilizer runoff from the Mississippi River Basin created a
dead zone of over 8,463 square miles. As you can see in the figure below, dead zones are
found in areas of high industrialization and population density around the world.
19. "Biogeochemical cycles" by Robert Bear, David Rintoul, Bruce Snyder, Martha
Smith-Caldas, Christopher Herren, and Eva Horne
Biogeochemical Cycles by Robert Bear
https://www.khanacademy.org/science/biology/ecology/biogeochemical-cycles/a/the-
phosphorous-cycle
https://www.slideshare.net/redbloodcelz/phosphorus-cycle?from_m_app=android